摘要:
Simple Summary: In this study, we conducted a comprehensive investigation of the fatty acid composition in Ningxiang pigs using a genome-wide association study. Our findings revealed a combination of previously reported and novel candidate genes associated with saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs). Notably, we identified significant single-nucleotide polymorphisms (SNPs) that are closely linked to specific fatty acids, and some of these genes explained substantial phenotypic variance. These noteworthy discoveries have the potential to significantly improve meat quality and fat deposition in Ningxiang pigs through targeted breeding approaches. Our research provides valuable insights into the intricate composition of fatty acids, thus offering practical implications for elevating meat quality and ultimately benefiting both the pig industry and consumers. The significance of this study is underscored by its potential to drive positive changes in society by promoting healthier and superior-quality pork products. Ningxiang pigs exhibit a diverse array of fatty acids, making them an intriguing model for exploring the genetic underpinnings of fatty acid metabolism. We conducted a genome-wide association study using a dataset comprising 50,697 single-nucleotide polymorphisms (SNPs) and samples from over 600 Ningxiang pigs. Our investigation yielded novel candidate genes linked to five saturated fatty acids (SFAs), four monounsaturated fatty acids (MUFAs), and five polyunsaturated fatty acids (PUFAs). Significant associations with SFAs, MUFAs, and PUFAs were found for 37, 21, and 16 SNPs, respectively. Notably, some SNPs have significant PVE, such as ALGA0047587, which can explain 89.85% variation in Arachidic acid (C20:0); H3GA0046208 and DRGA0016063 can explain a total of 76.76% variation in Elaidic Acid (C18:1n-9(t)), and the significant SNP ALGA0031262 of Arachidonic acid (C20:4n-6) can explain 31.76% of the variation. Several significant SNPs were positioned proximally to previously reported genes. In total, we identified 11 candidate genes (hnRNPU, CEPT1, ATP1B1, DPT, DKK1, PRKG1, EXT2, MEF2C, IL17RA, ITGA1 and ALOX5), six candidate genes (ALOX5AP, MEDAG, ISL1, RXRB, CRY1, and CDKAL1), and five candidate genes (NDUFA4L2, SLC16A7, OTUB1, EIF4E and ROBO2) associated with SFAs, MUFAs, and PUFAs, respectively. These findings hold great promise for advancing breeding strategies aimed at optimizing meat quality and enhancing lipid metabolism within the intramuscular fat (IMF) of Ningxiang pigs.
摘要:
Highly immunogenic nucleotide fragments from 3 genes of Mycoplasma hyopneumoniae strain 232 were selected using information software technology. After repeating each fragment three times, a total of 9 nucleotide fragments were joined together to form a new nucleotide sequence called Mhp2321092bp. Mhp2321092bp was directly synthesized and cloned into a pET100 vector and expressed in Escherichia coli. After purification, the proteins were successfully validated by SDS-PAGE and Western blotting using mouse His-tag antibody and pig anti-Mhp serum. BALB/c mice were intraperitoneally injected with purified proteins in the high-dose (100 & mu;g), medium-dose group (50 & mu;g) and low-dose (10 & mu;g) groups. Mice in each group were injected on day 1, day 8 and day 15 of feeding, respectively. Serum samples were collected from all mice on the day before immunization and on day 22 after immunization. The antibody level in the mouse serum was detected using western blotting using purified expressed proteins as antigens. IL-2, TNF-& alpha; and IFN-& gamma; were simultaneously detected in the mouse serum by ELISA. The results showed that the 60 kDa protein was successfully expressed and reacted specifically with the specific serum Mhp His-Tag mouse monoclonal antibody and pig anti-Mhp serum. From day 0 to day 22 of immunization, IFN-& gamma; increased from 269.52 to 467.74 pg/mL, IL-2 increased from 14.03 to 145.16 pg/mL, and TNF-& alpha; increased from 6.86 to 12.37 pg/mL. The IgG antibody in mice increased significantly from 0 day to day 22 after immunization. This study suggests that the expressed recombinant protein may serve as one of the novel vaccine candidates for Mhp.
通讯机构:
[Guanghui Chen] T;[Yue Wang] D;The Key Laboratory of Crop Germplasm Innovation and Resource Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Department of Agronomy, College of Agronomy, Hunan Agricultural University, Changsha 410128, China
摘要:
Abstract: Rice noodles are one of southern people’s favorite foods in China, so it is important to find the suitable raw rice for rice noodle making. To study the effects of different rice varieties on the quality of fresh wet rice noodles and to explore the relationship between the quality of the rice and the quality of the fresh wet rice noodles, this study to compare the 12 hybrid rice varieties as raw materials analyzed the differences in the cooking quality, texture index, and sensory score of fresh wet rice noodles using the principal component analysis, membership function, and cluster analysis. The results showed that the quality of fresh wet rice noodles prepared from different hybrid rice materials differed significantly. The fresh wet rice noodles made from Liangyou 5836 are of good quality, and they are mainly characterized by a low rate of broken noodles and spit pulp value, high rice noodle hardness, good rice noodle elasticity, strong rice noodle chewiness, and low adhesiveness. Moreover, its sensory evaluation is also better than that of other varieties. The comprehensive evaluation of 12 hybrid rice varieties by subordinate function analysis also showed that Liangyou 5836 was the best. In addition, through principal component analysis and gray analysis, it was found that 14 related indicators of rice quality and fresh wet rice noodle quality were concentrated into four categories, among which gel consistency best reflects the quality of rice and fresh wet rice noodles. Through comprehensive analysis, it was found that an amylose content of about 22% and a gel consistency of less than 40 mm can be used as core indicators to screen varieties suitable for making rice noodles. This study is of great significance for the selection of hybrid rice for both rice quality and fresh wet rice noodle quality. Keywords: diverse rice hybrids; fresh wet rice noodles; physicochemical in rice noodles; amylose content; gel consistency
作者机构:
[Guan, Chunyun; Zhang, Yin; Chen, Can; Huang, Huang; Xu, Ying] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;[Guan, Chunyun; Zhang, Yin; Chen, Can; Huang, Huang] Hunan Engn Res Ctr Rice Field Ecol Planting & Bree, Changsha 410128, Peoples R China.;[Li, Zhengyou] Fisheries Res Inst Guizhou Prov, Guiyang 550025, Peoples R China.;[Luo, Jun] Congjiang Agr & Rural Bur Guizhou Prov, Congjiang 557400, Peoples R China.;[Ren, Bo] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.
通讯机构:
[Huang Huang] C;College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Engineering Research Center of Rice Field Ecological Planting and Breeding, Changsha 410128, China
关键词:
rice-fish-duck symbiosis system;globally important agricultural heritage system;value;technology;challenge;countermeasure
摘要:
Abstract: The Chinese rice–fish–duck symbiosis system is one of the globally important agricultural heritage systems, with a history of nearly a thousand years. It embodies the wisdom of the Chinese ancestors and has huge comprehensive value, which has extremely important references for the sustainable development of agriculture. The technological core for the rice–fish–duck symbiosis system lies in the selection of rice, fish, and duck varieties and the coupling of agricultural operation time. In the process of modern agriculture, many varieties with high comprehensive benefits have also been applied to the rice–fish–duck system, and the rice–fish–duck model has been further developed. What is worrying is that the transfer of rural labor, the promotion of modern agricultural technology, the low degree of marketization and industrialization, the weakening of cultural awareness, and the change in climate have posed huge threats and challenges to the traditional Chinese rice–fish–duck system. The protection and development of the system are urgently needed. Therefore, we recommend delimiting active protection areas, establishing scientific research bases, improving relevant systems, developing rural tourism markets, and innovating farming technology, which also provide guidance for protecting and developing other agricultural cultural heritages. Keywords: rice–fish–duck symbiosis system; globally important agricultural heritage system; value; technology; challenge; countermeasure
作者机构:
[Liu, Shengjun; Ma, Longteng] Jiangxi Haida Feed Co Ltd, Nanchang 331700, Peoples R China.;[Zhang, Zixi; Ma, Longteng] Hunan Agr Univ, Coll Anim Sci & Technol, Anim Nutr Genome & Germplasm Innovat Res Ctr, Changsha 410128, Peoples R China.
通讯机构:
[Longteng Ma] J;Jiangxi Haida Feed Co., Ltd., Nanchang 331700, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要:
Abstract: Simple Summary Neonates are highly susceptible to intestinal infections; this has been generally ascribed to the immaturity of the immune system, but other factors might contribute. The composition of the gut microbiota is a key factor, as the microbiota protects the host against colonization by pathogens. The neonatal gut microbiota is less diverse and lacks two taxa that are dominant in older intestines: members of the orders Clostridiales and Bacteroidales. Microorganisms are closely related to the body’s physiological activities and growth and development of the body, and participate in many physiological metabolic activities. Analysis of the structure and source of early colonizing bacteria in the intestinal tract of humans and rodents shows that early colonizing bacteria in the intestinal tract of mammals have solid maternal characteristics, and maternal microbes play an essential role in the formation of progeny intestinal flora. Abstract Microorganisms are closely related to the body’s physiological activities and growth and development of the body, and participate in many physiological metabolic activities. Analysis of the structure and source of early colonizing bacteria in the intestinal tract of humans and rodents shows that early colonizing bacteria in the intestinal tract of mammals have solid maternal characteristics, and maternal microbes play an essential role in the formation of progeny intestinal flora. The placental microbiome, maternal microbiome and breast milk microbiome are currently hot topics in the field of life science. This paper discusses the vertical transmission and endogenous sources of the mother-to-piglet microbiome through these three pathways, aiming to provide a new research idea for intervention in the intestinal microbiome in young piglets. Keywords: microorganisms; vertical transmission; placental microbiome; maternal microbiome; breast milk microbiome
摘要:
The objective was to determine effects of cellulase, xylanase, and commercial fibrolytic enzymes on fermentation quality, aerobic stability, bacterial community, and in vitro degradation of mixed silages. Mixtures of alfalfa, wheat bran, and rice straw [80:15:5 on a fresh matter (FM) basis] were ensiled for 1, 3, 5, 7, 15, 30, and 45 d after treatment with: distilled water (control, C); cellulase (E); xylanase (X); or commercial fibrolytic enzymes (EX), with all enzyme preparations applied at 100 U/g FM. The 45-day silages were subjected to an in vitro degradation test. Each of the three enzyme-treated groups enriched relative abundance (RA) of Lactobacillus, Weissella, and Stenotrophomonas maltophilia, increased water soluble carbohydrate (WSC) concentrations, and extended aerobic stability over 384 h, but concurrently inhibited growth of undesirable microbes (i.e., Acinetobacter sp, Lelliottia amnigena, and Sphingomonas sp), reducing pH and concentrations of ammonia nitrogen (AN), butyric acid (BA) and propionic acid (PA). Compared to C, adding X or EX increased the RA of L. paralimentarius and L. parabrevis, enhanced accumulation of acetic acid (AA) and crude protein (CP), and reduced hemicellulose content. Furthermore, E group silage had the highest abundance of W. cibaria. In addition, EX enriched RA of Bacillus velezensis, reduced AN concentration, increased DM degradability, total VFA production, and gas production during in vitro incubation. In conclusion, addition of X or EX enhanced ensiling by enhancing concentrations of AA; however, EX was the most promising enzyme, based on reducing AN concentration and increasing DM content and DM degradability.
摘要:
Fruit cracking seriously affects the commercial value of table grapes. To explore whether cell wall disassembly influences grape berry cracking, first, the differences in the cell wall metabolism were compared between cracking-resistant "Shennongjinhuanghou" (SN) and cracking-susceptible "Xiangfei" (XF) varieties. Our results showed that cell wall disassembly events were extremely different between "SN" and "XF." The cracking-resistant "SN" had a higher pectinmethylesterase activity in the early stage and lower polygalacturonase, β-galactosidase, pectate lyase, and cellulase activities from veraison, cooperatively yielding higher ionically bound pectin, covalently bound pectin, hemicellulose, and lower water-soluble pectin, leading to a stronger skin break force and elasticity and conferring "SN" with higher cracking resistance. Furthermore, the function of the VvPL1 gene in fruit cracking was verified by heterologously transforming tomatoes. The transgenic experiment showed that overexpressed fruits had a higher activity of pectate lyase from the breaking stage and a lower level of covalently bound pectin, ionically bound pectin, cellulose, and hemicellulose and a higher level of water-soluble pectin at the red ripe stage, which resulted in a significantly reduced skin break force and flesh firmness and increased fruit cracking incidences. In conclusion, our results demonstrated that the cracking susceptibility of the grape berry is closely related to cell wall disassembly events and VvPL1 plays an important role in fruit cracking.
通讯机构:
[Songlin Sun] C;[Bo Hu] H;College of Mechanical and Electrical Engineering, Hunan Agriculture University, Changsha 410012, China<&wdkj&>Hunan Provincial Key Laboratory of Intelligent Manufacturing Technology for High-Performance Mechanical Equipment, Changsha 410114, China<&wdkj&>College of Automotive and Mechanical Engineering, Changsha University of Science and Technology, Changsha 410114, China
摘要:
Abstract: Bearings and other revolute pairs in gear drives will form clearances due to wear after working for a long time, which will aggravate the vibration and noise of the gear. Therefore, taking the gear drives with revolute pair clearance as the research object, the calculation formulas of the backlash and contact ratio is derived, and the time-varying mesh stiffness model considering the clearance is presented in this work. Then, a nonlinear dynamic model for the gear drives with revolute pair clearance is proposed by combining the backlash, contact ratio and the time-varying mesh stiffness. The effects of the clearance on the meshing stiffness, load sharing factor and nonlinear behavior are investigated. Results indicate that the revolute pair clearance weakens the meshing stiffness and increases working hours in single teeth contact area, thereby increasing the risk of premature failure of the gear drive. In addition, the revolute pair clearance has negligible effect on the nonlinear behavior of gear pair in low-speed running, but it can obviously change the bifurcation behavior and aggravate the vibration of the gear drive. Keywords: revolute pair clearance; gear drives; nonlinear dynamics; time-varying mesh stiffness
摘要:
The accurate measurement of soil organic matter (SOM) is vital for maintaining soil quality. We present an innovative model for SOM prediction by integrating spectral and profile features. We use PCA, Lasso, and SCARS methods to extract important spectral features and combine them with profile data. This hybrid approach significantly improves SOM prediction across various models, including Random Forest, ExtraTrees, and XGBoost, boosting the coefficient of determination (R2) by up to 26%. Notably, the ExtraTrees model, enriched with PCA-extracted features, achieves the highest accuracy with an R2 of 0.931 and an RMSE of 0.068. Compared with single-feature models, this approach improves the R2 by 17% and 26% for PCA features of full-band spectra and profile features, respectively. Our findings highlight the potential of feature integration, especially the ExtraTrees model with PCA-extracted features and profile features, as a stable and accurate tool for SOM prediction in extensive study areas.
摘要:
BACKGROUND: Diquat is a common environmental pollutant, which can cause oxidative stress in humans and animals. Diquat exposure cause growth retardation and intestinal damage. Therefore, this study was performed to investigate the effects of melatonin on diquat challenged piglets. RESULTS: The result showed that dietary supplementation with 2 mg/ kg melatonin significantly increased the average daily gain (ADG), and feed conversion rate in piglets. Melatonin increased antioxidants capacity, and improves intestinal epithelial barrier function of duodenum and jejunum in piglets. Moreover, melatonin also regulates the expression of immune and antioxidant related genes. Furtherly, melatonin also alleviates diquat induced growth retardation, and anorexia in diquat-challenged piglets. Moreover, melatonin also increased antioxidants capacity, and ameliorate diquat induced intestinal epithelial barrier injury. Melatonin regulated the expression of MnSOD in intestinal, and immune related genes. CONCLUSION: In conclusion, dietary supplementary with 2 mg/kg melatonin increased antioxidant capacity to ameliorate diquat-induced oxidative stress, alleviates intestinal epithelial barrier injury, and increased growth performance in weaned piglets. This article is protected by copyright. All rights reserved.
通讯机构:
[Luo, S ] H;[Wei, ZS ; Luo, S] A;Aarhus Univ, Ctr Water Technol WATEC, Univ Byen 36, DK-8000 Aarhus C, Denmark.;Aarhus Univ, Dept Biol & Chem Engn, Univ Byen 36, DK-8000 Aarhus C, Denmark.;Hunan Agr Univ, Coll Environm & Ecol, Changsha 410128, Peoples R China.
关键词:
Micropollutants;O(2) adsorption and activation;Oxygen vacancies;Visible light;Z-scheme
摘要:
Visible light photocatalysis is widely considered a sustainable approach to break down micropollutants without chemical addition. To promote the output of photogenerated carriers under visible light, a Z-scheme plasmonic photocatalyst Bi-CeO(2)/Ag(0)/BiO(2) was designed and fabricated to activate dissolved oxygen in water for micropollutant degradation. The doped Bi not only improved the separation of electron-hole, but also narrowed the band gap of CeO(2) to enhance its absorption of visible light. Notably, metallic silver (Ag(0)) works as an electronic transmission vehicle from Bi-CeO(2) to BiO(2) in a Z-scheme mechanism. Likewise, the surface plasmon resonance effect of Ag(0) also enhanced the absorption of visible light. Furthermore, the Bi doping induced abundant surface oxygen vacancies on CeO(2) for enhanced capability and selectivity towards O(2) adsorption and activation, which favored the generation of O(2)(•-) by photogenerated electrons to degrade sulfamethoxazole, enrofloxacin, and bisphenol A. Theoretical calculation results also confirmed the O(2)(•-)-driven degradation pathway for sulfamethoxazole. Therefore, the Z-scheme Bi-CeO(2)/Ag(0)/BiO(2) not only extends the photocatalytic reactivity of CeO(2)-based catalysts to the visible light range, but also provides a chemical-free method to effectively degrade micropollutants.
作者机构:
[Chen, Yinke; Peng, Yan; Teng, Zhenning; Duan, Meijuan; Ye, Nenghui; Qin, Zhonge; Liu, Bohan; Yu, Huihui; Ye, NH; Meng, Shuan; Lv, Jiahan; Duan, MJ] Hunan Agr Univ, Coll Agron, Hunan Prov Key Lab Rice Stress Biol, Changsha 410128, Peoples R China.;[Teng, Zhenning; Zhang, Jianhua; Yu, Huihui] Chinese Univ Hong Kong, Sch Life Sci, Hong Kong 999077, Peoples R China.;[Teng, Zhenning; Zhang, Jianhua; Yu, Huihui] Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong 999077, Peoples R China.;[He, YC; He, Yuchi] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430000, Peoples R China.;[Zhang, Jianhua; Ye, Nenghui; Ye, NH] Hong Kong Baptist Univ, Dept Biol, Hong Kong 999077, Peoples R China.
通讯机构:
[He, YC ; Zhang, JH; Ye, NH ; Duan, MJ] H;[Zhang, JH ] C;Hunan Agr Univ, Coll Agron, Hunan Prov Key Lab Rice Stress Biol, Changsha 410128, Peoples R China.;Chinese Univ Hong Kong, Sch Life Sci, Hong Kong 999077, Peoples R China.;Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong 999077, Peoples R China.
摘要:
Low-temperature germination (LTG) is an important agronomic trait for direct-seeding cultivation of rice (Oryza sativa). Both OsMYB30 and OsTPP1 regulate the cold stress response in rice, but the function of OsMYB30 and OsTPP1 in regulating LTG and the underlying molecular mechanism remains unknown. Employing transcriptomics and functional studies revealed a sugar signaling pathway that regulates seed germination in response to low temperature (LT). Expression of OsMYB30 and OsTPP1 was induced by LT during seed germination, and overexpressing either OsMYB30 or OsTPP1 delayed seed germination and increased sensitivity to LT during seed germination. Transcriptomics and qPCR revealed that expression of OsTPP1 was upregulated in OsMYB30-overexpressing lines but downregulated in OsMYB30-knockout lines. In vitro and in vivo experiments revealed that OsMYB30 bound to the promoter of OsTPP1 and regulated the abundance of OsTPP1 transcripts. Overaccumulation of trehalose (Tre) was found in both OsMYB30- and OsTPP1-overexpressing lines, resulting in inhibition of alpha-amylase 1a (OsAMY1a) gene during seed germination. Both LT and exogenous Tre treatments suppressed the expression of OsAMY1a, and the osamy1a mutant was not sensitive to exogenous Tre during seed germination. Overall, we concluded that OsMYB30 expression was induced by LT to activate the expression of OsTPP1 and increase Tre content, which thus inhibited alpha-amylase activity and seed germination. This study identified a phytohormone-independent pathway that integrates environmental cues with internal factors to control seed germination. Low temperature increases the abundance of a transcription factor, which activates the biosynthesis of trehalose and consequently inhibits seed germination by impeding alpha-amylase activity in rice.
摘要:
INTRODUCTION: The Tibetan Plateau is characterized by low temperature and hypoxia. N-carbamylglutamic acid (NCG) can increase blood oxygen saturation, and have the potential to be used to prevent the high-altitude hypoxia stress state of cows. However, its beneficial effect on the rumen microbiota of Holstein dairy cows remains unclear. METHODS: Hence, the experiments 12 multiparous (parity ranged from 2 to 7) Holstein dairy cows (413.0 ± 42 kg) were randomly assigned to 2 treatments with 6 replicates in each treatment: basal diet (CON, control group) and basal diet plus 20 g/d/cow of NCG (NCG, experiment group), respectively. To study the effects of dietary NCG supplementation on rumen microbiota of Holstein dairy cows in Tibet. The experiment lasted for 45 days, with 15 days of pre-feeding and 30 days of formal trail period. RESULTS: The results showed that ruminal NH(3)-N concentration in NCG group was lower (p < 0.05) than that in the CON group, while molar proportion of acetic acid and total volatile fatty acid (VFA) concentration were increased (p < 0.05) with the addition of NCG. Microbial diversity increased (p < 0.05) in NCG group, with Bacteroidetes, Firmicutes, and Patescibacteria as the most abundant phyla. The KEGG pathway analysis showed that the potential function of ruminal bacteria was mainly enriched in metabolism (carbohydrates, amino acids, lipids, energy, and nucleotides) and genetic information processing (replication, repair, and translation). CONCLUSION: In conclusion, NCG can improve rumen nitrogen utilization, total VFA and acetic acid production, and increase rumen microbial diversity, all of which could make the introduced Holstein dairy cows to better adapt to the harsh environment in Tibet and improve their production performance.
摘要:
The present study was conducted to evaluate the regulatory actions and underlying mechanisms of butyrate on the inflammatory response and tight junction (TJ) disruption in bovine mammary epithelial cells (BMECs). Results showed that butyrate declined histone deacetylase 3 (HDAC3) expression, blocked NF-kappa B activation, and thus suppressed inflammatory cytokine production in gamma-d-glutamyl-meso-diaminopimelic acid (iE-DAP)-triggered BMECs. Butyrate also depressed the protein abundance of myosin light chain kinase (MLCK), elevated the expression of TJ proteins, and restored the cellular distribution of TJ proteins and the barrier function of epithelial cells. HDAC3 overexpression abolished the protective effects of butyrate. In conclusion, butyrate alleviated the iE-DAP-induced inflammatory response and TJ injury by blocking NF-kappa B activation and decreasing inflammatory cytokine production and MLCK expression in a HDAC3-dependent manner. Our finding provides a mechanistic basis for further exploring the regulatory effects of butyrate on the mammary inflammatory response.
通讯机构:
[Chunyun Guan; Mei Guan] C;College of Agriculture, Hunan Agricultural University, Hunan Branch of National Oilseed Crops Improvement Center, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha 410128, China
关键词:
high-oleic-acid rapeseed;lncRNA-mRNA;lipid metabolism;seed development
摘要:
Abstract: A high oleic acid content is considered an essential characteristic in the breeding of high-quality rapeseed in China. Long-chain non-coding RNA (lncRNA) molecules play an important role in the plant’s growth and its response to stress. To better understand the role of lncRNAs in regulating plant reproductive development, we analyzed whole-transcriptome and physiological data to characterize the dynamic changes in lncRNA expression during the four representative times of seed development of high- and low-oleic-acid rapeseed in three regions. We identified 21 and 14 lncRNA and mRNA modules, respectively. These modules were divided into three types related to region, development stages, and material. Next, we analyzed the key modules related to the oil content and the oleic acid, linoleic acid, and linolenic acid contents with physiological data and constructed the key functional network analysis on this basis. Genes related to lipid metabolism, such as 3-ketoacyl-CoA synthase 16 (KCS16) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), were present in the co-expression network, suggesting that the effect of these genes on lipid metabolism might be embodied by the expression of these lncRNAs. Our results provide a fresh insight into region-, development-stage-, and material-biased changes in lncRNA expression in the seeds of Brassica napus. Some of these lncRNAs may participate in the regulatory network of lipid accumulation and metabolism, together with regulated genes. These results may help elucidate the regulatory system of lncRNAs in the lipid metabolism of high-oleic-acid rapeseed seeds. Keywords: high-oleic-acid rapeseed; lncRNA–mRNA; lipid metabolism; seed development
通讯机构:
[Jiangchi Fei] C;College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, China
关键词:
Plastic residue;Crop type;Irrigation regime;Plastisphere;Bacterial community
摘要:
Rice plants are highly sensitive to high-temperature stress, posing challenges to grain yield and quality. However, the impact of high temperatures on the quality of high-quality hybrid rice during the booting stage, as well as the differing effects of the booting and grain-filling stages on grain quality, are currently not well-known. Therefore, four high-quality hybrid rice were subjected to control (CK) and high-temperature stress during the booting (HT1) and grain-filling stages (HT2). Compared to the control, HT1 significantly reduced the spikelets panicle(-1) (16.1%), seed setting rate (67.5%), and grain weight (7.4%), while HT2 significantly reduced the seed setting rate (6.0%) and grain weight (7.4%). In terms of quality, both HT1 and HT2 significantly increased chalkiness, chalky grain rate, gelatinization temperature, peak viscosity (PV), trough viscosity (TV), final viscosity (FV), and protein content in most varieties, and significantly decreased grain length, grain width, total starch content, and amylose content. However, a comparison between HT1 and HT2 revealed that the increase in chalkiness, chalky grain rate, PV, TV, and FV was greater under HT2. HT1 resulted in a greater decrease in grain length, grain width, total starch content, and amylose content, as well as an increase in protein content. Additionally, HT1 led to a significant decrease in amylopectin content, which was not observed under HT2. Therefore, future efforts in breeding and cultivating high-quality hybrid rice should carefully account for the effects of high temperatures at different stages on both yield and quality.
摘要:
The input of exogenous organic matter could affect the transformation of soil carbon (C) and nitrogen (N), and their C- and N-priming effects (CPE and NPE) play a key role in the balance of soil C and N. However, little is known about how the interaction effect between straw and straw biochar regulates CPE and NPE. Therefore, we conducted a 90-day incubation experiment, which included five treatments: no straw and straw biochar (CK), 1.5% straw (S), 0.53% straw biochar (B), 1.5% straw + 0.53% straw biochar (SB), and 1.5% straw + 1.06% straw biochar (SB1). Our findings revealed that cumulative soil CO2 emissions were increased by 95.52–216.53% through the short-term input of exogenous organic matter input; however, this trend gradually weakened with decreasing dissolved organic C (DOC) content. The cumulative NPE generated by the addition of exogenous organic matter was much smaller than the cumulative CPE. Under the B and S treatments, the cumulative CPE and NPE were negative throughout the entire incubation period. The SB treatment remarkably boosted the microbial biomass nitrogen (MBN) content; however, the SB1 treatment was more effective in inhibiting soil C and N mineralization processes than SB treatment. Moreover, the cumulative CPE and NPE were mainly regulated by N. We conclude that the combination of straw and straw biochar preferentially stimulated soil C mineralization, but that this effect decreased with time, which may be due to the consumption of labile DOC caused by the initial positive CPE, while soil N mineralization had a lag effect.