摘要：
Abstract Background and Objective Texture is critical in determining the eating quality of cooked rice and is dependent on physiochemical properties of rice grains. In this study, relationships between texture properties of cooked rice with grain amylose and protein content were evaluated across 25 high eating quality indica rice varieties. Findings Grain amylose and protein content ranged from 13.2% to 20.0% and from 5.37% to 7.77%, with means of 17.6% and 6.43%, respectively. The ranges of hardness, springiness, cohesiveness, resilience, and chewiness of cooked rice were 425–1074 g, 0.672–0.812, 0.447–0.632, 0.278–0.457, and 130–480 g, with means of 710 g, 0.768, 0.570, 0.394, and 320 g, respectively. Correlation plot analysis and partial correlation analysis showed that all five texture properties of cooked rice were significantly related to grain amylose content, whereas the relationships between each of the five texture properties and grain protein content were not significant or much less significant than the relationships between texture properties and grain amylose content. Conclusions High eating quality indica rice varieties have diverse texture properties when cooked. Selection of texture properties of cooked rice should focus on grain amylose content rather than grain protein content for high eating quality indica rice. Significance and Novelty This study identifies the critical physiochemical property determining cooked rice texture in high eating quality indica rice.
摘要：
The outstanding electrical conductivity of transition metal carbides Ti(3)C(2)T(x)(MXene) makes it as an excellent electron transfer medium for fabrication of efficient catalysts. However, the poor stability of MXene may restrict its application. Herein, a novel silver nanoparticles/reduced MXene nanocomposite (AgNPs/rMXene) catalyst was prepared by using L-arginine (L-Arg) as a green reducing agent. In the AgNPs/rMXene catalyst, the silver nanoparticles (AgNPs) and reduced MXene (rMXene) acted as catalytic active species and electron transfer medium, respectively. The composite catalyst exhibited superior catalytic activity in the conversion of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP), and the conversion frequency (TOF) was high up to 1109.4 h(-1). Notably, the composite catalyst also showed high stability due to the reduction of L-Arg (i.e. the repair of defect groups on MXene surface). The conversion efficiency for 4-NP reduction by AgNPs/rMXene was high up to 90% after five recycles. This present study offers a simple and green approach for the design and development of efficient MXene-based catalysts.
通讯机构：
[Yan, LH; Li, YL ; Yan, LH ] H;Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.;Hunan Bot Garden, Changsha 410128, Peoples R China.;Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Changsha 410005, Peoples R China.;Minist Educ, Engn Res Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.
关键词：
salt stress;Machilus faberi Hemsl;adaptability;plant growth response;physiological indicators;biochemical indicators
摘要：
Adversity stress is the main environmental factor limiting plant growth and development, including salt and other stress factors. This study delves into the adaptability and salt tolerance mechanisms of Machilus faberi Hemsl, a species with potential for cultivation in salinized areas. We subjected the plants to various salt concentrations to observe their growth responses and to assess key physiological and biochemical indicators. The results revealed that under high salt concentrations (500 and 700 mmol(-1)/L), symptoms such as leaf yellowing, wilting, and eventual death were observed. Notably, plant height and shoot growth ceased on the 14th day of exposure. Chlorophyll content (a, b, total a + b, and the a/b ratio) initially increased but subsequently decreased under varying levels of salt stress. Similarly, the net photosynthetic rate, stomatal conductance, leaf water content, and root activity significantly declined under these conditions. Moreover, we observed an increase in malondialdehyde levels and relative conductivity, indicative of cellular damage and stress. The activity of superoxide dismutase and ascorbate peroxidase initially increased and then diminished with prolonged stress, whereas peroxidase activity consistently increased. Levels of proline and soluble protein exhibited an upward trend, contrasting with the fluctuating pattern of soluble sugars, which decreased initially but increased subsequently. In conclusion, M. faberi exhibits a degree of tolerance to salt stress, albeit with growth limitations when concentrations exceed 300 mmol(-1)/L. These results shed light on the plant's mechanisms of responding to salt stress and provide a theoretical foundation for its cultivation and application in salt-affected regions.
关键词：
Clostridium butyricum;mycelium of Phellinus igniarius;colitis;intestinal flora;serum metabolome
摘要：
Clostridium butyricum (CB) and Phellinus igniarius (PI) have anti-inflammatory, immune regulation, anti-tumor, and other functions. This study aimed to explore the therapeutic effect of CB and mycelium of PI (MPI) alone and in combination on colitis mice induced by dextran sodium sulfate (DSS). Mice were randomly assigned to five groups: (1) control (CTRL), (2) DSS, (3) CB, (4) MPI, and (5) CB + MPI (CON). The weight of the mice was recorded daily during the experiment, and the length of the colon was measured on the last day of the experiment. The colons were collected for hematoxylin and eosin staining, colon contents were collected for intestinal flora analysis, and serum was collected for metabolite analysis. The results showed that compared with the DSS group, CB, MPI, and CON treatments inhibited the weight loss and colon length shortening caused by DSS, significantly increased the concentrations of interleukin (IL)-4, IL-10, and superoxide dismutase, and significantly decreased the concentrations of IL-6, tumor necrosis factor-α, and myeloperoxidase. Gene sequence analysis of 16S rRNA showed that CB, MPI, and CON treatments changed the composition and structure of intestinal microorganisms. Metabolome results showed that CB, MPI, and CON treatments changed serum metabolites in DSS-treated mice, including dodecenoylcarnitine, L-urobilinogen, and citric acid. In conclusion, CB, MPI, and CON treatments alleviated DSS-induced colitis in mice by regulating intestinal flora and metabolites, with the CON group having the best effect.
通讯机构：
[Zhang, XM ] H;Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.
关键词：
First principles;Precipitate/matrix interface;Diffusion;Vanadium alloys
摘要：
To gain insights into the nucleation of H and He bubbles at the precipitate/matrix interface, first-principles calculations have been performed to investigate the trapping and diffusion behaviors of H and He at the TiC/ V interface. As for the site preference, the calculation shows that H and He prefer to occupy the V-side tetrahedral site at the interface rather than the center of the interface. Considering the H and He clusters formation, H clusters are more likely to aggregate between the first and second slab, whereas He clusters tend to aggregate at the V matrix of the first slab. The diffusion of H and He across the TiC(110)/V(100) interface demonstrates that H and He atoms can reach the favorable segregation sites at the interface from the V bulk by overcoming energy barriers of 0.23 eV and 0.59 eV, respectively. Meanwhile, trapped H and He atoms are difficult to escape from the interface. H and He atoms are, however, highly susceptible to diffusion along the interface, which means that the diffusion path can act as a fast diffusion channel. Additionally, doping Ti can facilitate the trapping of H and He at the TiC/V interface, with no significant change in the diffusion barrier for H, while reducing the diffusion barrier for He to 0.45 eV.
摘要：
The close association of soil organic carbon (SOC) with Fe oxides is an important stabilization mechanism for soil organic matter (SOM) against biodegradation. Soil conditioners are of great importance in improving soil quality and soil health. Yet it remains unclear how different conditioners would affect the fractionation of SOC, particularly the Fe-bound organic carbon (Fe-OC). Field-based experiments were conducted in farmland to explore the fractionation of organic carbon (OC) and Fe oxides under the effects of three different soil conditioners (mineral, organic, and microbial conditioners). The results showed that all soil conditioners increased the total OC and Fe-OC contents, with the contribution of Fe-OC to total OC increasing from 1.57% to 2.99%. The low OC/Fe molar ratio indicated that surface adsorption played a crucial role in soil Fe-OC accumulation. Nuclear magnetic resonance (NMR) results suggested that soil conditioner altered the composition of SOM, accelerating O-alkyl C degradation and increasing recalcitrant alkyl C and aromatic C sequestration. Scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS) analysis indicated that all conditioners promoted the association of OC and Fe oxides. Furthermore, comprehensive analysis of 13C isotope and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy showed that the mineral conditioner enhanced the association of microbial-derived OC and Fe oxides, whereas the organic conditioner increased the association of plant-derived OC with Fe oxides. These findings provide important insights into the potential mechanisms through which soil conditioners regulate the stability of OC and guide agricultural management.
通讯机构：
[Ding, SH ] H;Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China.
关键词：
Active food packaging;Strawberry fruit;UV and microbial defense functions
摘要：
Multi-active food packaging was prepared for strawberry fruit preservation where epigallocatechin gallate (EGCG)-containing pectin matrix and natamycin (NATA)-containing chitosan (CS) matrix were utilized to complete LBL electrostatic self-assembly. The results showed that the physicochemical properties of the multi-active packaging were closely related to the addition of NATA and EGCG. It was found that NATA and EGCG were embedded in the CS/pectin matrix through intermolecular hydrogen bonding interactions. The CN/PE 15% multi-active films prepared based on the spectral stacking theory formed a barrier to UV light in the outer layer, exhibited excellent NATA protection under UV light exposure conditions at different times, and provided long-lasting and sustained bacterial inhibition in the inner layer. In addition, the CN/PE 15% multi-active packaging extended the shelf life of strawberry at room temperature compared with the control samples. In conclusion, the developed CN/PE 15% packaging provided potential applications for multi-active food packaging materials.
通讯机构：
[Hu, SW ] W;[Liu, G ] H;Wenzhou Med Univ, Peoples Hosp Wenling 1, Wenling Hosp, Orthopaed Ctr, Wenzhou, Zhejiang, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
关键词：
L. plantarum;dexamethasone;differential metabolites;food active ingredients;glucocorticoids;gut microbes;osteoporosis;probiotics
摘要：
INTRODUCTION: Osteoporosis, one of the most common non-communicable human diseases worldwide, is one of the most prevalent disease of the adult skeleton. Glucocorticoid-induced osteoporosis(GIOP) is the foremost form of secondary osteoporosis, extensively researched due to its prevalence.Probiotics constitute a primary bioactive component within numerous foods, offering promise as a potential biological intervention for preventing and treating osteoporosis. This study aimed to evaluate the beneficial effects of the probiotic Lactobacillus plantarum on bone health and its underlying mechanisms in a rat model of glucocorticoid dexamethasone-induced osteoporosis, using the osteoporosis treatment drug alendronate as a reference. METHODS: We examined the bone microstructure (Micro-CT and HE staining) and analyzed the gut microbiome and serum metabolome in rats. RESULTS AND DISCUSSION: The results revealed that L. plantarum treatment significantly restored parameters of bone microstructure, with elevated bone density, increased number and thickness of trabeculae, and decreased Tb.Sp. Gut microbiota sequencing results showed that probiotic treatment increased gut microbial diversity and the ratio of Firmicutes to Bacteroidota decreased. Beneficial bacteria abundance was significantly increased (Lachnospiraceae_NK4A136_group, Ruminococcus, UCG_005, Romboutsia, and Christensenellaceae_R_7_group), and harmful bacteria abundance was significantly decreased (Desulfovibrionaceae). According to the results of serum metabolomics, significant changes in serum metabolites occurred in different groups. These differential metabolites were predominantly enriched within the pathways of Pentose and Glucuronate Interconversions, as well as Propanoate Metabolism. Furthermore, treatment of L. plantarum significantly increased serum levels of Pyrazine and gamma-Glutamylcysteine, which were associated with inhibition of osteoclast formation and promoting osteoblast formation. Lactobacillus plantarum can protect rats from DEX-induced GIOP by mediating the "gut microbial-bone axis" promoting the production of beneficial bacteria and metabolites. Therefore L. plantarum is a potential candidate for the treatment of GIOP.
摘要：
Theobromine is an important quality component in tea plants (Camellia sinensis), which is produced from 7-methylxanthine by theobromine synthase (CsTbS), the key rate-limiting enzyme in theobromine biosynthetic pathway. Our transcriptomics and widely targeted metabolomics analyses suggested that CsMYB114 acted as a potential hub gene involved in the regulation of theobromine biosynthesis. The inhibition of CsMYB114 expression using antisense oligonucleotides (ASO) led to a 70.21% reduction of theobromine level in leaves of the tea plant, which verified the involvement of CsMYB114 in theobromine biosynthesis. Furthermore, we found that CsMYB114 was located in the nucleus of the cells and showed the characteristic of a transcription factor. The dual luciferase analysis, a yeast one-hybrid assay, and an electrophoretic mobility shift assay (EMSA) showed that CsMYB114 activated the transcription of CsTbS, through binding to CsTbS promoter. In addition, a microRNA, miR828a, was identified that directly cleaved the mRNA of CsMYB114. Therefore, we conclude that CsMYB114, as a transcription factor of CsTbS, promotes the production of theobromine, which is inhibited by miR828a through cleaving the mRNA of CsMYB114.
期刊：
Journal of Materials Chemistry C,2024年 ISSN：2050-7526
通讯作者：
Zhang, Sijin;Mao, X;Zhang, SJ
作者机构：
[Mao, Xia; Yin, Jiamai; Ouyang, Sha; Zhou, Zhi; Zhang, SJ; Su, Liujuan; Zhang, Sijin] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;[Zhang, SJ; Zhang, Sijin; Yao, Maohai; Wang, Guichao] Hunan Rare Earth Met Mat Res Inst Co Ltd, 108 Longyuan 2nd Rd,Longping High Tech Pk, Changsha, Hunan, Peoples R China.;[Mao, Xia; Yin, Jiamai; Ouyang, Sha; Zhou, Zhi; Su, Liujuan; Yang, Jiayi] Hunan Opt Agr Engn Technol Res Ctr, Changsha 410128, Peoples R China.;[Yang, Jiayi] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;[Molokeev, Maxim S.] Fed Res Ctr KSC SB, RAS, Lab Crystal Phys, Krasnoyarsk 660036, Russia.
通讯机构：
[Mao, X ; Zhang, SJ ; Zhang, SJ] H;Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;Hunan Rare Earth Met Mat Res Inst Co Ltd, 108 Longyuan 2nd Rd,Longping High Tech Pk, Changsha, Hunan, Peoples R China.;Hunan Opt Agr Engn Technol Res Ctr, Changsha 410128, Peoples R China.
摘要：
Phytochrome PFR plays a key role in plant photomorphogenesis, and its perception of far-red light is essential, but how to obtain an efficient far-red phosphor to achieve accurate light filling remains a huge challenge. In this study, Gd1-y-zAl3-x(BO3)4:xCr3+,yLu3+,zSm3+ (GAB:xCr3+,yLu3+,zSm3+) series phosphors were synthesized by a high-temperature solid-state method. By doping Lu3+, the emission intensity of Cr3+ could increase as high as 20%. With the introduction of Sm3+, the emission intensity of Cr3+ was further increased by 29%. Particularly, the emission spectra can be tuned by varying the concentration ratio of Sm3+ and Cr3+, more suitable for the absorption spectrum of PFR. Moreover, the internal quantum yield and external quantum yield of GL0.1AB:0.03Cr3+ and GL0.1AB:0.03Cr3+,0.003Sm3+ were 83.1% and 24.7% and 78.1% and 26.3%, respectively. There were high anti-thermal quenching properties in the prepared phosphors at 423 K, with 107.6% (GAB:0.03Cr3+), 103.1% (GL0.1AB:0.03Cr3+), and 102.7% (GL0.1AB:0.003Sm3+,0.03Cr3+). Finally, the phosphors were made into pc-LED devices, which can realize the adjustable orange-red and far-red luminescence and meet the needs of plant lighting applications. In the light-regulated plant growth experiment, compared with the control group, far-red light promoted root growth in plants, confirming the application potential of the prepared phosphors in indoor plant cultivation. Structural modification and spectral optimization of luminescence characteristics of far-red phosphors for plant growth.
摘要：
Emotional mimicry plays a vital role in understanding others’ emotions and has been found to be modulated by social contexts, especially group membership. However, the neural mechanisms underlying this modulation remain unclear. We explored whether and how group membership modulated emotional mimicry using a multimodal method combining facial electromyography (fEMG) and electroencephalography (EEG). We instructed participants to passively view dynamic emotional faces (happy vs. angry) of others (in-group vs. out-group) and simultaneously recorded their fEMG and EEG responses. Then, we conducted combined analyses of fEMG-EEG by splitting the EEG trials into two mimicry intensity categories (high-intensity mimicry vs. low-intensity mimicry) according to fEMG activity. The fEMG results confirmed the occurrence of emotional mimicry in the present study but failed to find a group membership effect. However, the EEG results showed that participants mimicked in-group happiness and anger more than out-group. Importantly, this in-group preference involved different neural mechanisms in happiness and anger mimicry. In-group preference for happiness mimicry occurred at multiple neural mechanisms such as N1 (at P7, Pz, and P8), P2 (at Pz and P8), N2 (at P8), and P3 (at P7, Pz, and P8); in-group preference for anger mimicry occurred at P1 (at P7) and P2 (at Pz). Our findings provide new neural evidence for the effect of group membership on emotional mimicry by uncovering the temporal dynamics of this effect.
摘要：
This study aimed to investigate the effects of supplementing laying hen diets with Radix Isatidis Polysaccharide (RIPS) on egg quality, immune function, and intestinal health. The research was conducted using 288 Hyland Brown hens, which were randomly assigned to four dietary treatments: control (without RIPS), low dose (200g/t), medium dose (500g/t), and high dose (1000g/t) of RIPS. Each dietary treatment was administered to eight replicates of nine hens for nine weeks. The results revealed that RIPS inclusion in diets significantly improved egg quality parameters such as egg shape index, yolk color, haugh unit, and protein height (P<0.05). Additionally, RIPS supplementation enhanced immune function as evidenced by an alteration in serum biochemical parameters, an increase in the spleen index, and a decrease in the liver index. Further, an evaluation of intestinal health showed that RIPS fortified the intestinal barrier, thus increasing the population of beneficial intestinal bacteria and reducing the abundance of harmful ones. Such mechanisms promoted intestinal health, digestion, and nutrient absorption, ultimately leading to enhanced egg quality. In conclusion, supplementing laying hen diets with RIPS has been demonstrated to improve egg quality by boosting immunity and optimizing intestinal digestion and absorption.
摘要：
An automatic, rapid and non-targeted detection method for multi-pesticides in plant-derived foods was developed by gas chromatography-mass spectrometry and chemometrics. In this method, a novel algorithm named moving window iterative target transformation factor analysis was proposed. Although there are challenges of peak overlapping and background interference, the retention time and corrected mass spectra of unknown pesticides can be automatically obtained through iteration calculation in the 'moving window' with reference to the pesticide mass spectral library. One mixed pesticide standard and nine varieties of plant-derived foods were investigated with the proposed method. By contrast, a fast temperature programme was used to shorten detection time compared to the standard temperature programme. For the mixed standard, the mass spectra and retention times of all 39 pesticides were successfully obtained from the overlapping signal. Furthermore, all spiked pesticides were successfully detected in plant-derived foods within 10min using a fast temperature programme.
摘要：
Understanding the influencing effect of meteorological factors and air pollutants in the campus plot and the relationship between them is an important topic in the planning and design of campus green space. The changes of pollutant concentrations and meteorological factors in campus green space have certain patterns and specific influencing factors. In this study, we selected four sample plots in Nanjing Forestry University as the research objects, and collected various environmental parameters of the four plots on July 25, 2022. The results showed that the main influences of meteorological factors are the type of the underlying surface of the site, the degree of plant canopy density and the shade coverage area of the building. These factors mainly have a great influence on the value of temperature and humidity. The comprehensive influencing factors can be concluded that the cooling and humidifying effect of the site is ranked as follows: forest > lawn > asphalt road > concrete Square. The main influencing factors of pollutants are: illumination, wind speed, temperature and relative humidity. Among them, illumination and temperature have a negative correlation with PM(2.5), wind speed and relative humidity have a positive correlation with PM(2.5). Our research shows that the adjustment of campus green space factors can reduce the concentration of pollutants by changing the meteorological factors.
期刊：
Journal of Environmental Management,2024年352:119851 ISSN：0301-4797
通讯作者：
Zhu, ML
作者机构：
[Xu, Junbing] Minjiang Univ, NewHuadu Business Sch, Fuzhou City, Fujian Province, Peoples R China.;[Zhu, Minling] Hunan Agr Univ, Econ Coll, Changsha City, Hunan Province, Peoples R China.;[Zhan, Shaoguo] Chinese Acad Social Sci, Inst Chinese Borderland Studies, Beijing City, Peoples R China.;[Xu, Junbing] Area 1,200,Xiyuangong Rd, Fuzhou City, Fujian Province, Peoples R China.;[Zhu, Minling] Hunan Agr Univ, Coll Agr, Room 219,North 10th Teaching Bldg,1 Yuanda Rd, Changsha City 410125, Hunan Province, Peoples R China.
通讯机构：
[Zhu, ML ] H;Hunan Agr Univ, Econ Coll, Changsha City, Hunan Province, Peoples R China.
摘要：
With global warming, cities are vulnerable to extreme weather, increasing the climate risk to cities worldwide. Although existing literature has examined the ex-post impacts of extreme weather, it is less clear how climate risk affects cites before extreme weather occur. To lower the risk of urban waterlogging, which is caused by extreme weather, and improve the ability of cities to adapt to extreme weather, China launched the Sponge City Project (SCP) in 2013 to manage the urban stormwater and waterlogging. Adopting the SCP pilot in China as a quasi-natural experiment, we examine the impact of the climate risk caused by urban stormwater and waterlogging on the house price with the difference-in-differences (DID) method. We find that after implementing the SCP pilot program, the house price in pilot cities increased significantly because of the improvement in cities' resilience to climate risk. Additionally, this effect was only demonstrated in cities with a high waterlogging risk. For SCP pilot cities with lower waterlogging risk, the house price is not significantly affected by SCP implementation. This indicates that the house price in China is sensitive to the climate risk caused by the urban stormwater and waterlogging. Our findings also contribute to the understanding of the significance of the climate risk management, and provided theoretical evidence for urban governance.
摘要：
This study designed surface flow constructed wetlands (SFCWs) with Myriophyllum aquaticum (M. aquaticum) to evaluate how different influent C/N ratios (0:1 (C0N), 5:1 (C5N), 10:1 (C10N), and 15:1 (C15N)) affect pollutant removal, greenhouse gas (GHG) emissions, and microbial communities. The results showed that effluent ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N), and total nitrogen (TN) concentrations decreased, but effluent chemical oxygen demand (COD) concentration increased with increasing influent C/N ratios. The highest removal rates of TN (73.17%) and COD (74.56%) were observed with C5N. Regarding GHG emissions, a few changes in CO2 fluxes were caused by the influent C/N ratio, whereas CH4 fluxes obviously increased with the increasing influent C/N ratio. The highest N2O emission occurred with C0N (211.03 +/- 44.38 mg-N<middle dot>m(-2)<middle dot>h(-1)), decreasing significantly with higher C/N ratios. High-throughput sequencing revealed that different influent C/N ratios directly influenced the microbial distribution and composition related to CH4 and N2O metabolism in SFCWs. The highest abundance (46.24%) of denitrifying bacteria (DNB) was observed with C5N, which helped to achieve efficient nitrogen removal with a simultaneous reduction in N2O emissions. Methanogen abundance rose with higher C/N ratios, whereas methanotrophs peaked under C5N and C10N conditions. Additionally, the random forest model identified influent C/N ratio and Rhodopseudomonas as primary factors influencing CH4 and N2O emissions, respectively. This highlights the importance of the influent C/N ratio in regulating both pollutant removal and GHG emissions in constructed wetlands.
摘要：
Cadmium (Cd) contamination is a widespread environmental issue. There is a lack of knowledge about the impacts of applying arbuscular mycorrhizal fungi (AMF) and biochar, either alone or in their combination, on alleviating Cd phytotoxicity in Ligustrum lucidum. Therefore, a pot experiment was conducted in a greenhouse, where L. lucidum seedlings were randomly subjected to four regimes of AMF treatments (inoculation with sterilized AMF, with Rhizophagus irregularis, Diversispora versiformis, alone or a mixture of these two fungi), and two regimes of biochar treatments (with or without rice-husk biochar), as well as three regimes of Cd treatments (0, 15, and 150mgkg(-1)), to examine the responses of growth, photosynthetic capabilities, soil enzymatic activities, nutritional concentrations, and Cd absorption of L. lucidum plants to the interactive effects of AMF, biochar, and Cd. The results demonstrated that under Cd contaminations, AMF alone significantly increased plant total dry weight, soil pH, and plant nitrogen (N) concentration by 84%, 3.2%, and 13.2%, respectively, and inhibited soil Cd transferring to plant shoot by 42.2%; biochar alone significantly enhanced net photosynthetic rate, soil pH, and soil catalase of non-mycorrhizal plants by 16.4%, 9%, and 11.9%, respectively, and reduced the soil Cd transferring to plant shoot by 44.7%; the additive effect between AMF and biochar greatly enhanced plant total dry weight by 101.9%, and reduced the soil Cd transferring to plant shoot by 51.6%. Furthermore, dual inoculation with D. versiformis and R. irregularis conferred more benefits on plants than the single fungal species did. Accordingly, amending Cd-contaminated soil with the combination of mixed-fungi inoculation and biochar application performed the best than either AMF or biochar alone. These responses may have been attributed to higher mycorrhizal colonization, soil pH, biomass accumulation, and biomass allocation to the roots, as well as photosynthetic capabilities. In conclusion, the combined use of mixed-fungi involving D. versiformis and R. irregularis and biochar addition had significant synergistic effects on enhancing plant performance and reducing Cd uptake of L. lucidum plants in Cd-contaminated soil.
关键词：
Flavor substances;Flavor-producing bacteria;Levilactobacillus spicheri;Microbial community structure;P-cresol;Sour bamboo shoots
摘要：
Identifying key flavor compounds and their producing bacteria in sour bamboo shoots is crucial for flavor stabilization and industrial production. This study analyzed 15 traditional sour bamboo shoot samples from northern Guangdong to determine key flavor substances and microbial community. Results showed key flavor substances were acetic acid (RTC >= 50% in 10 samples), lactic acid (RTC >= 50% in 5 samples), and p-cresol (ROC >= 93%). Lactobacillus (ARA: 54.62%) was the dominant genus, significantly correlated with p-cresol (r = 0.80, p <= 0.01). Levilactobacillus (ARA: 3.33%) was positively correlated with lactic acid and p-cresol (r = 0.78, p <= 0.01; r = 0.66, p <= 0.01). Lentilactobacillus (ARA: 4.29%) was positively correlated with acetic acid (r = 0.85, p <= 0.01). Levilactobacillus was isolated, screened, identified, and its ability to produce key flavor substances was tested. Four strains of Levilactobacillus spicheri and their mixed strains produced lactic acid (10.12-16.62 g/kg), acetic acid (10.21-21.60 g/kg), and p-cresol (25.67-143.87 mg/kg). This is the first report of Levilactobacillus spicheri producing p-cresol.
摘要：
A survey was conducted of the volatile organic compounds (VOCs) released from sources of solvent use, industry activities and vehicle emissions in Guiyang, a capital city of China. Samples were collected by canisters and analyzed by GC-MS-FID. The species profiles of VOCs emitted from sources were obtained. Results showed that xylenes, ethylbenzene, acetone and dichloromethane were the characteristics species for painting, 2-propanol and ethyl acetate for printing, a-pinene for solid wood furniture manufacturing, and 2-butanone for biscuit baking. These characteristics species could be as tracers for the sources respectively. In most of samples from the solvent use, the benzene/toluene (B/T) ratio was less than 0.3, indicating that the ratio could be as the indicator for tracing the solvent use related sources. The results also suggested that the toluene/xylene (T/X) ratio be as the indicator to distinguish the VOCs sources of painting ( < 2) from the printing ( > 2). Aromatics contributed the most to ozone formation potential (OFP) of most painting and non-paper printing sources, and oxygen-containing VOCs (OVOCs) were major species contributing to OFP of the sources from food production and paper printing. The OFP of the VOCs emissions from vehicle in tunnels and from other manufactures were dominated by both aromatics and alkenes. The a-pinene could explain 56.94% and 32.54% of total OFP of the VOCs sources from filing cabinet and solid wood furniture manufacturing, which was rarely been involved in previous studies of VOCs source profiles, indicating that the species of concern for VOCs sources are still insufficient at present.(c) 2023 The Research Center for Eco-Environmental Sciences, Chinese Academy of
摘要：
Previous research has shown that the pathogenicity and appressorium development of Magnaporthe oryzae can be inhibited by the ATP synthase subunit beta (Atp2) present in the photosynthetic bacterium Rhodopseudomonas palustris. In the present study, transgenic plants overexpressing the ATP2 gene were generated via genetic transformation in the Zhonghua11 (ZH11) genetic background. We compared the blast resistance and immune response of ATP2-overexpressing lines and wild-type plants. The expression of the Atp2 protein and the physiology, biochemistry, and growth traits of the mutant plants were also examined. The results showed that, compared with the wild-type plant ZH11, transgenic rice plants heterologously expressing ATP2 had no significant defects in agronomic traits, but the disease lesions caused by the rice blast fungus were significantly reduced. When infected by the rice blast fungus, the transgenic rice plants exhibited stronger antioxidant enzyme activity and a greater ratio of chlorophyll a to chlorophyll b. Furthermore, the immune response was triggered stronger in transgenic rice, especially the increase in reactive oxygen species (ROS), was more strongly triggered in plants. In summary, the expression of ATP2 as an antifungal protein in rice could improve the ability of rice to resist rice blast.
