期刊:
Science of The Total Environment,2024年912:169005 ISSN:0048-9697
通讯作者:
Hu, Dongnan;Zhang, Manyun
作者机构:
[Liu, Yaohui; Fan, Haoqi; Zuo, Jing; Zhang, Ling; Zhang, Manyun] Jiangxi Provincial Key Laboratory of Silviculture, College of Forestry, Jiangxi Agricultural University, Nanchang 330000, China;[Xu, Zhihong; Bai, Shahla Hosseini] Centre for Planetary Health and Food Security, School of Environment and Science, Griffith University, Brisbane, Queensland 4111, Australia;[Hu, Dongnan] Jiangxi Provincial Key Laboratory of Silviculture, College of Forestry, Jiangxi Agricultural University, Nanchang 330000, China. Electronic address: dnhu98@jxau.edu.cn;[Zhang, Manyun] Centre for Planetary Health and Food Security, School of Environment and Science, Griffith University, Brisbane, Queensland 4111, Australia;[Zhang, Manyun] College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China. Electronic address: manyunzhang@126.com
通讯机构:
[Hu, Dongnan] J;[Zhang, Manyun] C;Jiangxi Provincial Key Laboratory of Silviculture, College of Forestry, Jiangxi Agricultural University, Nanchang 330000, China. Electronic address:;College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China. Electronic address:
摘要:
Biological nitrogen fixation and nitrification inhibitor applications contribute to improving soil nitrogen (N) availability, however, free-living N fixation affected by nitrification inhibitors has not been effectively evaluated in soils under different weed management methods. In this study, the effects of the nitrification inhibitors dicyandiamide (DCD) and 3, 4-dimethylpyrazole phosphate (DMPP) on the nitrogenase, nifH gene,and diazotrophic communities in soils under different weed management methods (AMB, weeds growth without mowing or glyphosate spraying; GS, glyphosate spraying; MSG, mowing and removing weeds and glyphosate spraying; and WM, mowing aboveground weeds) were investigated. Compared to the control counterparts, the DCD application decreased soil nitrogenase activity and nifH gene abundance by 4.5% and 37.9%, respectively, under the GS management method, and the DMPP application reduced soil nitrogenase activity by 20.4% and reduced the nifH gene abundance by 83.4% under the MSG management method. The application of nitrification inhibitors significantly elevated soil NH(4)(+)-N contents but decreased NO(3)(-)-N contents, which had adverse impacts on soil nifH gene abundance and nitrogenase activity. The nifH gene abundances were also negatively impacted by dissolved organic N and Geobacter but were positively affected by available phosphorus and diazotrophic community structures. Nitrification inhibitors significantly inhibited Methylocella but stimulated Rhizobiales and affected soil diazotrophic communities. The nitrification inhibitors DCD and DMPP significantly altered soil diazotrophic community structures, but weed management outweighed nitrification inhibitors in reshaping soil diazotrophic community structures. The non-targeted effects of the nitrification inhibitors DMPP and DCD on soil free-living N fixation were substantially influenced by the weed management methods.
摘要:
Previous epidemiological and animal studies have showed the lipid metabolic disruption of antimicrobial triclocarban (TCC) and triclosan (TCS). However, the present in vivo researches were mainly devoted to the hepatic lipid metabolism, while the evidence about the impacts of TCC/TCS on the adipose tissue is very limited and the potential mechanism is unclear, especially the molecular initiation events. Moreover, little is known about the toxic difference between TCC and TCS. This study aimed to demonstrate the differential adipogenic activity of TCC/TCS as well as the potential molecular mechanism via peroxisome proliferator-activated receptors (PPARα/β/γ). The in vitro experiment based on 3T3-L1 cells showed that TCC/TCS promoted the differentiation of preadipocytes into mature adipocytes at nanomolar to micromolar concentrations, which was approach to their human exposure levels. We revealed for the first time by reporter gene assay that TCC could activate three PPARs signaling pathways in a concentration-dependent manner, while TCS only activate PPARβ. The molecular docking strategy was applied to simulate the interactions of TCC/TCS with PPARs, which explained well the different PPARs activities between TCC and TCS. TCC up-regulated the mRNA expression of three PPARs, but TCS only up-regulated PPARβ and PPARγ significantly. Meanwhile, TCC/TCS also promoted the expression of adipogenic genes targeted by PPARs to different extent. The cellular and simulating studies demonstrated that TCC exerted higher adipogenic effects and PPARs activities than TCS. Our mice in vivo experiment showed that TCC could lead to adipocyte size increase, adipocyte lipid accumulation growing, fat weight and body weight gain at human-related exposure levels, and high fat diet exacerbated these effects. Moreover, male mice tended to be more susceptible to TCC induced obesogenic effect than female mice. This work highlights the potential obesogenic risks of TCC/TCS via PPARs signaling pathways, and TCC deserves more concerns for its higher activity.
作者机构:
[Zhao, Jihui; Tang, Shengsong; Zeng, Luting; Zhao, Xuhong; Ning, Qian; Huang, Ruilei; Yi, Yi] Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China;[Tang, Shengsong; Ning, Qian] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Tang, Shengsong] Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, and Institute of Pharmacy & Pharmacology, University of South China, Hengyang 421001, China. Electronic address: tangshengsong@hunau.edu.cn
通讯机构:
[Tang, Shengsong] H;Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, and Institute of Pharmacy & Pharmacology, University of South China, Hengyang 421001, China. Electronic address:
摘要:
Cancer immunotherapy, particularly with immune checkpoint inhibitors, has revolutionized the paradigm of cancer treatment. Nevertheless, the efficacy of cancer immunotherapy remains limited in most clinical settings due to the lack of a preexisting antitumor T-cell response in tumors. Therefore, the clinical outcomes of cancer immunotherapy must be improved crucially. With increased awareness of the importance of the innate immune response in the recruitment of T cells, as well as the onset and maintenance of the T cell response, great interest has been shown in activating the cGAS-STING signaling pathway to awaken the innate immune response, thereby orchestrating both innate and adaptive immune responses to induce tumor clearance. However, tumor cells have evolved to overexpress ectonucleotide pyrophosphate phosphodiesterase 1 (ENPP1), which degrades the immunotransmitter 2',3'-cGAMP and promotes the production of immune-suppressing adenosine, resulting in inhibition of the anticancer immune response in the tumor microenvironment. Clinically, ENPP1 overexpression is closely associated with poor prognosis in patients with cancer. Conversely, depleting or inhibiting ENPP1 has been verified to elevate extracellular 2',3'-cGAMP levels and inhibit the generation of adenosine, thereby reinvigorating the anticancer immune response for tumor elimination. A variety of ENPP1 inhibitors have recently been developed and have demonstrated significant promise for cancer immunotherapy. In this review, we provide an overview of ENPP1, dissect its immunosuppressive mechanisms, and discuss the development of ENPP1 inhibitors with the potential to further improve the efficacy of cancer immunotherapy.
摘要:
Cadmium (Cd) is a highly toxic heavy metal that can be readily absorbed by plants and enriched in human body. Rice (Oryza sativa L.) yield and grain quality are affected by excessive Cd in the soil. Therefore, understanding the mechanisms of Cd absorption, accumulation and detoxification in the root apex is crucial for developing low-Cd rice cultivars. After Cd treatment, Cd concentration in rice root tips (RT) was 1.4 times higher than that in basal roots (BR). To uncover the distinct molecular responses to Cd toxicity, we conducted transcriptomic, proteomic, and metabolomic analyses on the two root sections. The results revealed that the RT exhibited 1.2-2.0 fold higher transcript or protein abundance of several Cd-related transporters than the BR, including Nramp1, Nramp5, IRT1, and HMA3, thereby contributing to more Cd accumulation in the RT. Furthermore, multi-omics analysis unveiled that the RT had enhanced activity in 'phenylpropanoid metabolism', 'AsA-GSH cycle' and 'tryptophan metabolism', conferring the stronger antioxidant system. While the BR showed higher activation in 'cell wall remodeling' and 'terpenoid biosynthesis'. This comprehensive study provides insights into the regulatory network of genes, proteins and metabolites involved in the differential responses to Cd toxicity between rice root tips and mature zones.
期刊:
Journal of Hazardous Materials,2024年462:132740 ISSN:0304-3894
通讯作者:
Zhou, Nan
作者机构:
[Li, Shikai; Peng, Zhengjie; Wen, Yujiao; Zhou, Zhi; Su, Lezhu; Wang, Yifan; Liu, Meng] Hunan Engineering Research Center for Biochar, School of Chemistry and Materials Science, College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha 410128, China;[Zhou, Nan] Hunan Engineering Research Center for Biochar, School of Chemistry and Materials Science, College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha 410128, China. Electronic address: zhounan@hunau.edu.cn
通讯机构:
[Zhou, Nan] H;Hunan Engineering Research Center for Biochar, School of Chemistry and Materials Science, College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha 410128, China. Electronic address:
摘要:
Neither chemical nor physical adsorption play well in heavy metals remediation in acid soil due to the competing behavior of abundant protons, where stable chelators that can be reused are of significant demand. Herein, biochar with abundant nitro and carboxyl groups is prepared, which can be assembled into self-supporting electrode. Under the catalyzation of electricity, the surface decorated -NO(2) on the biochar can be in situ transformed into -NH(2). Combined with the carboxyl group that attached on the same carbon atom, a special α-amino acid-like structure modified biochar (α-AC@BC) can be successfully constructed. Due to the strong affinity between the α-amino acid-like ligand and heavy metals, this α-AC@BC exhibits high removal efficiencies of 83.41%, 80.94%, 92.54% and 77.05% for available copper, cadmium, lead and zinc respectively, even in a strong acid soil with low pH of 4. After four adsorption-desorption cycles, the α-AC@BC could still eliminate 83.88% of copper. The high adsorption energy among -NH(2), -COOH and heavy metals (-2.99eV for copper, -1.90eV for lead, -1.30eV for zinc and -0.91eV for cadmium) could form steady coordination structure to guarantee a highly practical application potential of α-AC@BC in strong acid soil. This study provides a novel concept for the decontamination of multiple heavy metal polluted acid soil.
期刊:
Frontiers in Ecology and Evolution,2024年11:1308310 ISSN:2296-701X
作者机构:
School of Marxism, Hunan University of Science and Technology, China;School of Marxism, Hunan University of Finance and Economics, China;Economic College, Hunan Agricultural University, China
关键词:
Dongting Lake area;Ecological environment;Zoning control;Influencing factors;ecological restoration
摘要:
Introduction: Protecting the ecosystem of the Dongting Lake area is of utmost importance for maintaining ecological balance and achieving human well-being.
摘要:
center dot Sugar transporter proteins (STPs) play critical roles in regulating plant stress tolerance, growth, and development. However, the role of STPs in regulating crop yield is poorly understood.center dot This study elucidates the mechanism by which knockout of the sugar transporter OsSTP15 enhances grain yield via increasing the tiller number in rice.center dot We found that OsSTP15 is specifically expressed in the shoot base and vascular bundle sheath of seedlings and encodes a plasma membrane-localized high-affinity glucose efflux transporter. OsSTP15 knockout enhanced sucrose and trehalose-6-phosphate (Tre6P) synthesis in leaves and improved sucrose transport to the shoot base by inducing the expression of sucrose transporters. Higher glucose, sucrose, and Tre6P contents were observed at the shoot base of stp15 plants. Transcriptome and metabolome analyses of the shoot base demonstrated that OsSTP15 knockout upregulated the expression of cytokinin (CK) synthesis-and signaling pathway-related genes and increased CK levels.center dot These findings suggest that OsSTP15 knockout represses glucose export from the cytoplasm and simultaneously enhances sugar transport from source leaves to the shoot base by promoting the synthesis of sucrose and Tre6P in leaves. Subsequent accumulation of glucose, sucrose, and Tre6P in the shoot base promotes tillering by stimulating the CK signaling pathway.
期刊:
Advanced Energy Materials,2024年 ISSN:1614-6832
通讯作者:
Fu, Lijun;Wu, YP;Ozoemena, KI
作者机构:
[Ye, Jilei; Liu, Lili; Yuan, Xuelong; Fu, Lijun; Yuan, Xinhai; Zhang, Shuaishuai; Wu, Yuping; Fu, LJ] Nanjing Tech Univ, Coll Chem Engn, Sch Energy Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China.;[Chen, Peng] Northeastern Univ, Minist Educ, Key Lab Ecol Met Multimet Minerals, Sch Met, Shenyang 110819, Peoples R China.;[Wu, Xiongwei] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;[Wang, Tao; Wu, Yuping] Southeast Univ, Sch Energy & Environm, Confucius Energy Storage Lab, Nanjing 210096, Jiangsu, Peoples R China.;[Ozoemena, Kenneth I.; Ozoemena, KI] Univ Witwatersrand, Mol Sci Inst, Sch Chem, Private Bag 3, ZA-2050 Johannesburg, South Africa.
通讯机构:
[Wu, YP ; Fu, LJ] N;[Ozoemena, KI ] U;Nanjing Tech Univ, Coll Chem Engn, Sch Energy Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China.;Southeast Univ, Sch Energy & Environm, Confucius Energy Storage Lab, Nanjing 210096, Jiangsu, Peoples R China.;Univ Witwatersrand, Mol Sci Inst, Sch Chem, Private Bag 3, ZA-2050 Johannesburg, South Africa.
关键词:
Al metal;aqueous rechargeable Al-ion battery;cobalt hexacyanoferrate;high energy density
摘要:
Like other aqueous batteries, aqueous rechargeable Al-ion batteries (ARAIBs) have attracted much attention due to their high safety and low cost. However, the low energy density of ARAIBs limits its popularization and application. In order to solve this problem, in addition to choosing Al metal as the negative electrode, it is also necessary to choose a suitable positive electrode material. Here, a cubic phase cobalt hexacyanoferrate (CoHCF) with excellent rate and cycling performance is used as the positive electrode material. Due to the reversible catalysis of the Cl-/Cl0 reaction at high potential in saturated AlCl3 solution, it has the characteristics of high capacity up to 103.5 mAh g-1. Combined with the Al metal as negative electrode, an ARAIB with an average discharge voltage of 1.56 V and an energy density of 155 Wh kg-1 is constructed, which shows outstanding cycling and rate performances. An aqueous rechargeable Al-ion battery (ARAIB) using a cubic phase cobalt hexacyanoferrate as positive electrode shows excellent rate and cycling performance together with an average discharge voltage of 1.56 V and an energy density of 155 Wh kg-1.image
摘要:
The high activity of water molecules results in a series of awful parasitic reaction, which seriously impede the development of aqueous zinc batteries. Herein, a new gel electrolyte with multiple molecular anchors is designed by employing natural biomaterials from chitosan and chlorophyll derivative. The gel electrolyte firmly anchors water molecules by ternary hydrogen bonding to reduce the activity of water molecules and inhibit hydrogen evolution reaction. Meanwhile, the multipolar charged functional groups realize the gradient induction and redistribution of Zn2+, which drives oriented Zn (002) plane deposition of Zn2+ and then achieves uniform Zn deposition and dendrite-free anode. As a result, it endows the Zn||Zn cell with over 1700 h stripping/plating processes and a high efficiency of 99.4% for the Zn||Cu cell. In addition, the Zn||V2O5 full cells also exhibit capacity retention of 81.7% after 600 cycles at 0.5 A g(-1) and excellent long-term stability over 1600 cycles at 2 A g(-1), and the flexible pouch cells can provide stable power for light-emitting diodes even after repeated bending. The gel electrolyte strategy provides a reference for reversible zinc anode and flexible wearable devices.
通讯机构:
[Zhou, N ] H;Hunan Agr Univ, Hunan Engn Res Ctr Biochar, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Peoples R China.
摘要:
Transition-metal-catalyzed reactive oxygen species (ROS) play an important role in existing bactericidal systems, but there are some problems, such as poor stability, ease of dissolution, and difficulty in reusing. To address the issue of stability, we have developed a sufficient electron-donating (SED) system. In the presence of the two most active transition metals with multiple valences, several as-formed redox pairs can provide sufficient electrons, and then effectively trigger the reduction of H2O/O-2 to produce O-1(2), <middle dot>OH and <middle dot>O-2(-) without any assistance from extra energy or other active oxidants. Consequently, the SED system showed 100% removal efficiency for Escherichia coli in 2 h, and also showed remarkable stability and reusability with 85% inactivation after 11 repetitions. It is worth noting that <middle dot>OH and O-1(2) play a major role in the bactericidal process. While <middle dot>O-2(-) mainly plays a role in promoting the formation of O-1(2) and <middle dot>OH.
摘要:
Abstract Existing studies on the environmental Kuznets curve (EKC) neglect the inverse effect of pollution transfer from environmental regulation interactions on pollution reduction from a risk analysis perspective. Based on the regional differentiated attitudes on the environmental regulation reached in risk communication by the risk awareness biases of multiple interest groups, this article clarifies the causality between risk communication and risk transfer based on multistakeholder engagement processes; furthermore, the article incorporates the simultaneous action of the technological innovation effect and pollution risk transfer effect to construct a spatial environmental hyperbolic model with a bidirectional correlation between pollution emissions and economic growth in different regions. To verify our model, we select the pollution from agricultural watersheds in China as a sample to examine the two inverse effects. The results demonstrate that (1) agricultural watershed pollution and economic growth show an inverted U‐shaped relation and a U‐shaped relation in the local region and adjacent regions, respectively; (2) the pollution reduction assessment of the classical EKC model can be largely attributed to pollution risk transfer behavior; and (3) the turning point of the U‐shaped curve appears earlier than that of the inverted U‐shaped curve in the spatial hyperbola model. The findings suggest that stakeholders should consider the risk awareness bias caused by the imbalance of regional economic development and the scenarios that provide a “haven” for pollution risk transfer. Moreover, our study expands the theoretical connotation of the classical EKC hypothesis and is more suitable for pollution reduction scenarios in developing countries.
摘要:
Lignin and cellulose are two essential elements of plant secondary cell walls that shape the mechanical characteristics of the culm to prevent lodging. However, how the regulation of the lignin and cellulose composition is combined to achieve optimal mechanical characteristics is unclear. Here, we show that increasing OsTCP19 expression in rice coordinately repressed lignin biosynthesis and promoted cellulose biosynthesis, resulting in enhanced lodging resistance. In contrast, repression of OsTCP19 coordinately promoted lignin biosynthesis and inhibited cellulose biosynthesis, leading to greater susceptibility to lodging. We found that OsTCP19 binds to the promoters of both MYB108 and MYB103L to increase their expression, with the former being responsible for repressing lignin biosynthesis and the latter for promoting cellulose biosynthesis. Moreover, up-regulation of OsTCP19 in fibers improved grain yield and lodging resistance. Thus, our results identify the OsTCP19-OsMYB108/OsMYB103L module as a key regulator of lignin and cellulose production in rice, and open up the possibility for precisely manipulating lignin-cellulose composition to improve culm mechanical properties for lodging resistance.
关键词:
Soil organic matter;Spatial prediction;Digital soil mapping;Sampling density optimization;Random forest
摘要:
Purpose Spatial prediction of soil organic matter (SOM) in cultivated land is crucial for evaluating soil productivity and its role in terrestrial carbon cycling. Cultivated soils in mountainous regions are commonly scattered on the footslope whereas those in the plain regions are continuously planar distributed; hence, they are quite different in the degree of variation in soil-forming factors and thereby the soil properties including SOM. Materials and methods In this study, we used the digital soil mapping approach (DSM) to predict SOM (0-20 cm) in cultivated soils in a hill-mountain region, Longshan County (LS), and a plain-platform region, Nanxian County (NX), which are both located at the same latitude in Southern China. By using 6746 and 9571 soil sampling points for LS and NX, respectively, together with 33 environmental covariates, the optimal spatial interpolation models and the reasonable sample strategy were carefully discussed. Results and discussion Descriptive statistical results showed that SOM in LS and NX were both moderate variations (coefficient variation, 0.34) and were approximately normal distribution. SOM in NX was strongly spatially dependent while SOM in LS was a moderate spatial dependence. The conditional Latin hypercube sampling (cLHS) was more appropriate compared with the Simple Random Sampling (SRS) as the sampling strategy. The optimal model for predicting cultivated land SOM was the Random Forest (RF) model for both LS and NX. The prediction accuracy was positively correlated with the sampling density. Specifically, to obtain a high prediction accuracy, the reasonable sampling density for SOM in LS should be controlled at >= 4.0 per km(2), higher than that in NX (>= 2.0 per km(2)). Conclusions The combination of cLHS and the RF model probably is the best choice for cultivated land SOM spatial prediction in different terrains. Therefore, our results provide a basis for future DSM of SOM in similar regions and help optimize soil sampling density.
作者机构:
[Huang, Jian-An; Liu, Zhong-Hua; Xiong, Li-Gui; Li, Juan; Huang, Wen-Feng] Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan, China;[Huang, Jian-An; Liu, Zhong-Hua; Xiong, Li-Gui; Li, Juan; Huang, Wen-Feng] National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan, China;[Huang, Jian-An; Liu, Zhong-Hua; Xiong, Li-Gui; Li, Juan; Huang, Wen-Feng] Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan, China;[Huang, Jian-An; Liu, Zhong-Hua; Li, Juan; Huang, Wen-Feng] Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, Hunan, China;[Xiong, Li-Gui] Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, Hunan, China. Electronic address: xiongligui@hunau.edu.cn
通讯机构:
[Xiong, Li-Gui] K;Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, Hunan, China. Electronic address:
摘要:
Phyllosphere microorganisms have recently attracted the attention of scientists studying plant microbiomes. The origin, diversity, functions, and interactions of phyllosphere microorganisms have been extensively explored. Many experiments have demonstrated seasonal cycles of phyllosphere microbes. However, a comprehensive comparison of these separate investigations to characterize seasonal trends in phyllosphere microbes of woody and herbaceous plants has not been conducted. In this review, we explored the dynamic changes of phyllosphere microorganisms in woody and non-woody plants with the passage of the season, sought to find the driving factors, summarized these texts, and thought about future research trends regarding the application of phyllosphere microorganisms in agricultural production. Seasonal trends in phyllosphere microorganisms of herbaceous and woody plants have similarities and differences, but extensive experimental validation is needed. Climate, insects, hosts, microbial interactions, and anthropogenic activities are the diverse factors that influence seasonal variation in phyllosphere microorganisms.
摘要:
Extruded yam flour was prepared at different feed moisture to improve its instant properties. The water solubility index (WSI) and water absorption index (WAI) were used to compare the instant properties of yam flour. Their chemical compositions, particle size distribution, crystalline structure, and microscopic forms were also analyzed to assess the effects of feed moisture on the instant properties of yam flour. We found that extrusion significantly improved the instant properties of yam flour, while the WSI value increased from 29.50% to 71.86% and the WAI value decreased from 387.88% to 228.06% with decreased feed moisture. Extrusion led to the degradation of total starch and amylopectin, and the contents of soluble substances increased markedly. Extrusion destroyed the granular and crystalline structures, which were reconstituted as amylose-lipid complexes with a significant decrease in relative crystallinity. Increasing the feed moisture was beneficial to the flow and color retention, while lower feed moisture was more favorable to enhance the instant properties.
摘要:
Cytochrome P450s play critical roles in the metabolic resistance of insecticides in insects. Previous findings showed that enhanced P450 activity was an important mechanism mediating indoxacarb resistance, and multiple P450 genes were upregulated in indoxacarb resistant strains of Spodoptera litura. However, the functions of these P450 genes in insecticide resistance remain unknown. Here, the P450 inhibitor PBO effectively decreased the resistance of S. litura to indoxacarb. Ten upregulated P450 genes were characterized, all of which were overexpressed in response to indoxacarb induction. Knockdown of nine P450 genes decreased cell viability against indoxacarb, and further silencing of three genes (CYP339A1, CYP340G2, CYP321A19) in larvae enhanced the sensitivity to indoxacarb. Transgenic overexpression of these three genes increased resistance to indoxacarb in Drosophila melanogaster. Moreover, molecular modeling and docking predicted that these three P450 proteins could bind tightly to indoxacarb and N-decarbomethoxylated metabolite (DCJW). Interestingly, these three P450 genes may also mediate cross-resistance to chlorantraniliprole, λ-cyhalothrin and imidacloprid. Additionally, heterologous expression and metabolic assays confirmed that three recombinant P450s could effectively metabolize indoxacarb and DCJW. This study strongly demonstrates that multiple overexpressed mitochondrial and microsomal P450 genes were involved in insecticide resistance in S. litura.
期刊:
Science of The Total Environment,2024年913:169601 ISSN:0048-9697
通讯作者:
Fu, Hui;Deng, Jianmin
作者机构:
[Fu, Hui] Ecology Department, College of Resources & Environments, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha 410128, PR China. Electronic address: huifu367@163.com;[Li, Youzhi; Wu, Aiping; Ge, Yili; Liu, Huanyao; Cai, Guojun; Yuan, Guixiang] Ecology Department, College of Resources & Environments, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha 410128, PR China;[Deng, Jianmin] Taihu Laboratory for Lake Ecosystem Research, State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, PR China. Electronic address: jmdeng@niglas.ac.cn;[Li, Wei] Research Institute of Ecology & Environmental Sciences, Nanchang Institute of Technology, Nanchang 330099, PR China;[Jeppesen, Erik] Department of Bioscience and Centre for Water Technology/WATEC, Aarhus University, Vejlsøvej 25, 8600 Silkeborg, Denmark
通讯机构:
[Deng, Jianmin] T;[Fu, Hui] E;Ecology Department, College of Resources & Environments, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha 410128, PR China. Electronic address:;Taihu Laboratory for Lake Ecosystem Research, State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, PR China. Electronic address:
摘要:
Understanding how phytoplankton interacts with local and regional drivers as well as their feedbacks is a great challenge, and quantitative analyses of the regulating role of human activities and climate changes on these feedback loops are also limited. By using monthly monitoring dataset (2000-2017) from Lake Taihu and empirical dynamic modelling to construct causal networks, we quantified the strengths of causal feedbacks among phytoplankton, local environments, zooplankton, meteorology as well as global climate oscillation. Prevalent bidirectional causal linkages between phytoplankton biomass (chlorophyll a) and the tested drivers were found, providing holistic and quantitative evidence of the ubiquitous feedback loops. Phytoplankton biomass exhibited the highest feedbacks with total inorganic nitrogen and ammonia and the lowest with nitrate. The feedbacks between phytoplankton biomass and environmental factors from 2000 to 2017 could be classified into two groups: the local environments (e.g., nutrients, pH, transparency, zooplankton biomass)-driven enhancement loops promoting the response of the phytoplankton biomass, and the climate (e.g., wind speed)-driven regulatory loops suppressing it. The two counterbalanced groups modified the emergent macroecological patterns. Our findings revealed that the causal feedback networks loosened significantly after 2007 following nutrient loading reduction and unsuccessful biomanipulation restoration attempts by stocking carp. The strength of enhancement loops underwent marked decreases leading to reduced phytoplankton responses to the tested drivers, while the climate (decreasing wind speed, warming winter)-driven regulatory loops increased- like a tug-of-war. To counteract the self-amplifying feedback loops, the present eutrophication mitigation efforts, especially nutrient reduction, should be continued, and introduction of alternative measures to indirectly regulate the critical components (e.g., pH, Secchi depth, zooplankton biomass) of the loops would be beneficial.