期刊论文

中文

园艺学报

0513-353X

2007

34

2

513-516

10.16420/j.issn.0513-353x.2007.02.051

本校为第一机构

以编号为B1 、N1 、Z1的瓜叶菊增殖期的愈伤组织和丛生芽为材料,用高温胁迫和羟脯氨酸 (HYP)分别作为直接和间接选择压,进行了瓜叶菊耐热无性系的离体筛选研究.结果表明:(1)离体直接筛选的适宜选择压为40℃16～20 h,HYP间接筛选的适宜浓度为30 mmmol*L-1;(2)经过直接选择、HYP与高温胁迫结合多次筛选获得了Z1-1-1和 N1-2-1 两个耐热无性系;(3) Z1-1-1 与对照母株Z1在叶片厚度、气孔指数、栅栏组织厚度/海绵组织厚度(TPT/TST)方面存在显著差异,成年植株Z1-1-1 和Z1在花的育性上也差异明显,Z1结实率85%以上,而Z1-1-1在人工授粉的情况下结实率也仅1.0%;N1-2-1和其对照母株N1在形态和花的育性上没有...

Using high temperature and HYP as direct and indirect selection stress, in vitro selection of heat-tolerant clonal lines from tussock plantlets and callus of senecio cruentus was carried out. The results showed that ： （ 1 ） 40℃ for 16 - 20 h and 30 mmol· L^-1 HYP were optimal direct and indirect selection stress pressure, respectively; （2） Two heat-tolerant clones （Z 1-1-1 and N 1-2-1 ） were obtained by using high temperature direct selection, HYP and high temperature selection; （3） The regenerated plants of Z 1-1-1 were different si...