期刊论文

中文

水产学报

1000-0615

2006

30

6

837-842

10.3321/j.issn:1000-0615.2006.06.020

国家863计划中试项目（N0：101-01-05） 湖南省重大科技专项子课题（湘03-NKY-1001）

本校为第一机构

1998年,张学文等[1]通过分子重组,将人α干扰素(Hu-IFNα)基因编码序列克隆到鲤β肌动蛋白基因启动子下游,构建了能够在鱼体内表达的Hu-IFN-α基因重组分子,然后通过显微注射法将重组基因注射入草鱼1～2细胞期的受精卵内,获得转抗病基因草鱼.此后,该项目研究小组开展了一系列研究[2-6],对转Hu-IFN-α基因草鱼接种草鱼出血病病毒(GCRV991)的攻毒实验表明:转Hu-IFN-α基因草鱼对草鱼出血病病毒的抗性比普通草鱼提高了66%.但是,目前的转基因技术还无法使外源基因在鱼体内进行定点整合,目的基因在鱼体内的整合形式难以确定.

The Hu-IFN-α gene, which was transducted into downstream promoter of β-actin gene of common carp （ Cyprinus carpio ）, was recombined by DNA recombination technology. These recombined genes were injected into 1 - 2 cell fertilized eggs of grass carp （ Ctenophatyngodon idellus） by micro-injection technology, we gained transgenetic fish by molecular detection methods. In order to analyse the genetic expression of tran- Hu-IFN-α gene gynogensis F1, which male individualization were gained by raising methyl- testosterone, molecular genetic ma...