期刊论文

The expression and transmission of immune factors between generations in maternal Ctenopharyngodon idella after immunization with GCRV attenuated vaccine

Xiao, T.

中文

水产学报

1000-0615

2017

41

8

1308-1318

10.11964/jfc.20161210651

现代农业产业技术体系建设专项（CARS-46-42）~~；

本校为第一且通讯机构

为探究GCRV弱毒疫苗母源性免疫的草鱼母本及其子代免疫因子（IgM、C3、LSZ）表达特性及代间传递效应,采用ELISA、Rt-q PCR等方法检测了草鱼母本产前40 d接种疫苗后,母本血液、子代早期发育阶段及2月龄幼鱼3种免疫因子的蛋白活性及基因表达水平。结果显示,经GCRV弱毒疫苗免疫的草鱼母本血液、早期胚胎及幼鱼阶段IgM蛋白活性均显著高于对照组样品。子代各阶段中,28日龄的夏花样品IgM蛋白活性水平最高,而5日龄水花样品中蛋白活性最低;从受精卵发育至3日龄水花阶段,实验组样品免疫因子C3和LSZ的蛋白活性均显著高于对照组;2组鱼中IgM、C3和LSZ蛋白的活性水平随着发育进行,总体上呈先下降后升高的趋势...

In order to investigate the expression characteristics and transmission effects of immune factors between the female parent and the progeny of Ctenopharyngodon idella, after being vaccinated with GCRV attenuated vaccine to female grass carp (40 days old). the protein activity and gene expression levels of IgM, C3 and LSZ were detected in the blood of female parent during the early development embryos of progeny and juvenile fish. The activities of IgM protein in the blood, early embryos and juvenile stage of grass carp were significantly higher than those in the control group by GCRV attenuate...