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Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds

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成果类型:
期刊论文
作者:
Peng, Qi;Hu, Yan;Wei, Ran;Zhang, Yuan;Guan, Chunyun;...
通讯作者:
Liu, Chunlin
作者机构:
[Guan, Chunyun; Peng, Qi; Wei, Ran; Hu, Yan; Liu, Chunlin] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Inst Oil Crops, Changsha 410128, Hunan, Peoples R China.
[Peng, Qi; Ruan, Ying; Zhang, Yuan] Hunan Agr Univ, Dept Dev Biol, Life Sci & Technol Coll, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Chunlin] H
Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Inst Oil Crops, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Brassica napus;Double-gene;Fusion fragment;RNAi;Fatty acid;Modification
期刊:
Plant Cell Reports
ISSN:
0721-7714
年:
2010
卷:
29
期:
4
页码:
317-325
基金类别:
National High Technology and Development Program of ChinaNational High Technology Research and Development Program of China [2008AA10Z152]; Development Plan of the State Key Fundamental Research of ChinaState Key Development Program for Basic Research of China [2006CB101603]
机构署名:
本校为第一且通讯机构
院系归属:
农学院
生物科学技术学院
摘要:
The fatty acid composition in the seed oil was significantly modified following the introduction of transgenes. To further enhance the desirable characteristics of rapeseed oil, it would be beneficial to develop a new approach for the simultaneous silencing of two or more target genes. Our goals in the current study were to (1) increase oleic acid to more than 75%, (2) reduce polyunsaturated fatty acids (PUFA) to about 10% and erucic acid to zero, and (3) accomplish these changes in a single-transformation event. In a single transformation, two...

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