期刊论文

Identification of Sweet Potato Leaf Curl Virus by Small RNA Deep Sequencing in Sweet Potato Germplasm

中文

分子植物育种

1672-416X

2019

17

11

3641-3649

10.13271/j.mpb.017.003641

国家现代农业产业技术体系(CARS-11-C-16) 湖南省自然科学基金(2016JJ6070) 湖南省农业科技创新资金(2017XC13) 长沙市甘薯工程技术研究中心(KQ1701024) 湖南省甘薯工程技术研究中心(2018TP2032)共同资助.

本校为第一机构

本研究利用Small RNA深度测序对长沙、永州、邵阳等地采集的甘薯种质资源病毒症状样品进行鉴定,发现样品中含有与甘薯曲叶病毒同源的系列.设计引物进行PCR扩增,鉴定出24个SPLCV-A C1基因片段序列,经NCBI-Blast比对,与10个不同株系SPLCV同源性达96％以上.为进一步获得SPLCV全长序列,设计背靠背引物,经PCR扩增、鉴定,获得3个SPLCV全长序列.系列进化分析显示,这3个SPLCV分离物聚为同一分支,其中SPLCV (76)序列大小2 774bp,与甘薯乔治亚曲叶病毒中国分离物、甘薯金脉病毒具有较高同源性;SPLCV (47)序列大小2 777 bp,与甘薯河南曲叶病毒分离物具有较高同源性;SPLCV (68)序列大小2 832 bp,与甘薯广西曲...

In this study, the virus symptom samples of sweet potato germplasm resources collected from Changsha, Yongzhou and Shaoyang were identified by small RNA deep sequencing, and it was found that the samples contained a series of homology sequences with sweet potato leaf curl virus. 24 SPLCV-AC1 gene fragments sequences were identified after amplified by PCR with designed primers, which had over 96% homology with 10 different lines SPLCV by NCBI-Blast comparison. In order to further obtain the full-length sequence of SPLCV, back-to-back primers were designed, and then three full-length sequences o...