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Identification and expression analysis of the gene associated with geosmin production in Lyngbya kuetzingii UTEX 1547 (cyanobacteria)

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成果类型:
期刊论文
作者:
Zhang, Ting*;Li, Deliang;Wang, Guiping;Song, Lirong;Li, Lin
通讯作者:
Zhang, Ting
作者机构:
[Zhang, Ting] Cent S Univ, Coll Chem & Chem Engn, Ctr Environm & Water Resources, Changsha 410083, Hunan, Peoples R China.
[Zhang, Ting; Wang, Guiping; Li, Deliang] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
[Li, Lin; Song, Lirong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
通讯机构:
[Zhang, Ting] C
Cent S Univ, Coll Chem & Chem Engn, Ctr Environm & Water Resources, Changsha 410083, Hunan, Peoples R China.
语种:
英文
关键词:
Biosynthesis;Geosmin;Geosmin synthase gene;Lyngbya kuetzingii
期刊:
Harmful Algae
ISSN:
1568-9883
年:
2014
卷:
39
页码:
127-133
基金类别:
This study was supported by the National Natural Science Foundation of China (Grant Nos. 31000183 and 31100282 ). The authors would like to thank Prof. Zhaoguang Yang and Xiaowan Li of Central South University for their valuable suggestions on the manuscript.[SS]
机构署名:
本校为其他机构
院系归属:
动物科学技术学院
摘要:
Cyanobacteria are the major producers of geosmin in natural waters. To identify a gene involved in geosmin biosynthesis in cyanobacteria, the polymerase chain reaction (PCR) was used to amplify a 2298-bp open reading frame (ORF) from the geosmin-producing cyanobacterium Lyngbya kuetzingii UTEX 1547. This ORF encoded a protein of 765 amino acids. Alignment of the deduced amino acid sequence demonstrated that geoL had high similarity to the corresponding genes of Oscillatoria sp. PCC 6506(100% identity), Calothrix sp. PCC 7507 (89%), Anabaena ucrainica CHAB 1432 (88%), A. ucrainica CHAB 2155 (87...

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