期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2006

32

3

292-295

10.3321/j.issn:1007-1032.2006.03.017

101-01-05:高比容电子铝箔的研究开发与应用项目

本校为第一机构

为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测．检测结果为：对1120尾转基因草鱼的PCR阳性检测，检出率为60％；258尾转基因草鱼杂交F1，阳性检测率为53．8％；36尾转基因草鱼雌核发育F1，阳性率为91．7％，说明Hu-IFN-α基因成功导入草鱼中，并能遗传给F1．

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp, PCR testing technology were employed to test the Hu-IFN-α gene＇s genetic expression in its cross F1 and gynogenesis F1 generation. The results indicated： 1） the rate of the PCR positive test for 1 120 transgene carps reached 60%; 2） the rate of the PCR positive test for 258 transgene carps＇ cross F1 generation reached 53.8%; 3） the rate of the PCR positive test for 36 transgene Carps＇ cytogenesis F1 generation reached 91.7%. It shows that ...