期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2008

34

4

474-477

101-01-05:国家高技术研究发展计划（863计划）

本校为第一机构

为快速获得转Hu-IFN-a基因抗病草鱼纯系,从转Hu-IFN-a基因草鱼中,挑选9尾高表达水平的雌鱼作为亲本,经人工雌核发育,得到子一代水花89尾,与普通草鱼的雌核发育结果相比,两者孵化率差异不显著,但畸形率前者比后者高.从雌核发育子一代鱼苗中随机选取45尾鱼苗投喂含甲基睾丸素的饲料,进行生理转雄性试验,获得生理转雄性鱼苗21尾.

In order to get pure Transgenic Hu-IFN-a gene grass carp and maintain the character of disease, 9 high espressed transgenic Hu-IFN-a gene Ctenophatyngodon idellus were choiced to served as parent of artificially induce gynogenesis, 89 transgenic Hu-IFN-a gene grass carp gynogenesic F1 have higher malformation rate than that of the general grass carp gynogenesic F1.45 grass carp were choiced randomly, and raised with methy-testosterone, 21 male i...