期刊论文

Effect of Age and Weanling on the Protegrin-1 Gene Expression in Femur Marrow of Piglets

中文

动物营养学报

1006-267X

2007

19

5

605-609

10.3969/j.issn.1006-267X.2007.05.014

湖南农业大学***基金项目（YJ0508） 湖南省自然科学基金项目（06JJ50070）资助

本校为第一机构

本文旨在研究不同日龄仔猪骨髓组织抗菌肽Protegrin-1（PG-1）mRNA表达的差异及断奶对其表达的影响。分别于0（出生当天）、3、14、23、28日龄随机屠宰杜长大公猪4头,采集股骨骨髓组织。以18S rRNA作内标,用一优化的RT-PCR（反转录-聚合酶链式反应）方法进行半定量分析。结果表明:不同日龄仔猪骨髓组织PG-1mRNA相对丰度有明显的差异（P

To study the Protegrin-1（PG-1） gene expression difference in femur marrow of piglets at different ages and effect of that by weaning, an optimized semi-quantitative RT-PCR was applied by using 18S rRNA as inner marker. Samples were taken at birth, 3, 14, 23, 28 days of age respectively. The results showed that age affected PG-1 mRNA levels in femur marrow significantly （P〈 0.05）, the expression of PG-1 mRNA was the lowest at birth, then increased remarkably. Weaning affected PG-1 mRNA levels significantly also （P〈0.05）. The PG-1 gene ex...