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Characterization of a RacGTPase up-regulated in the large yellow croaker Pseudosciaena crocea immunity

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成果类型:
期刊论文
作者:
Han, Fang;Wang, Xiaoqing;Yang, Qilian;Cai, Mingyi;Wang, Zhi Yong*
通讯作者:
Wang, Zhi Yong
作者机构:
[Han, Fang; Yang, Qilian; Wang, Zhi Yong; Cai, Mingyi] Jimei Univ, Coll Fisheries, Key Lab Sci & Technol Aquaculture & Food Safety, Xiamen 361021, Peoples R China.
[Wang, Xiaoqing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wang, Zhi Yong] J
Jimei Univ, Coll Fisheries, Key Lab Sci & Technol Aquaculture & Food Safety, Xiamen 361021, Peoples R China.
语种:
英文
关键词:
GTP-binding activity;Immunization;Large yellow croaker;RacGTPase;Up-regulated expression
期刊:
Fish & Shellfish Immunology
ISSN:
1050-4648
年:
2011
卷:
30
期:
2
页码:
501-508
基金类别:
National High-Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2006AA10A405]; Foundation for Innovation Research Team of Jimei University
机构署名:
本校为其他机构
院系归属:
动物科学技术学院
摘要:
The Rac proteins are members of the Rho family of small G proteins and are implicated in the regulation of several pathways, including those leading to cytoskeleton reorganization, gene expression, cell proliferation, cell adhesion and cell migration and survival. In this investigation, a Rac gene (named as LycRac gene) was obtained from the large yellow croaker and it was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-LycRac). Moreover, the GTP-binding assay showed that the LycRac protein had GTP-binding activit...

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