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Electrochemical Immunosensor Based on the Chitosan-Magnetic Nanoparticles for Detection of Tetracycline

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成果类型:
期刊论文
作者:
Liu, Xia*;Zheng, Shu;Hu, Yuxin;Li, Zongjun;Luo, Fang;...
通讯作者:
Liu, Xia
作者机构:
[He, Zao; Li, Zongjun; Hu, Yuxin; Luo, Fang; Liu, Xia; Zheng, Shu] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
[He, Zao; Li, Zongjun; Hu, Yuxin; Luo, Fang; Liu, Xia; Zheng, Shu] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredien, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Xia] H
Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
Hunan Coinnovat Ctr Utilizat Bot Funct Ingredien, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Chitosan;Electrochemical immunosensor;Fe 3 O 4 magnetic nanoparticle;Tetracycline
期刊:
Food Analytical Methods
ISSN:
1936-9751
年:
2016
卷:
9
期:
10
页码:
2972-2978
基金类别:
This work was financially supported by the Hunan Provincial Natural Science Foundation of China (No. 2015JJ3077), Special Fund for Agro-Scientific Research in the Public Interest (No. 201303084), and “1515” talent cultivation plan of Hunan Agricultural University.
机构署名:
本校为第一且通讯机构
院系归属:
食品科学技术学院
摘要:
In this paper, a low-cost, simple, and highly sensitive electrochemical immunosensor was fabricated for the tetracycline detection based on gold electrode-modified carboxyl-Fe3O4 nanoparticle (MNPs) by chitosan (CS) as linker. The anti-tetracycline monoclonal antibody (Ab) was immobilized on the modified electrode surface. The binding of tetracycline to Ab was analyzed by differential pulse voltammetry. Here, MNPs were used as the signal amplifier to improve the sensitivity of the immunosensor. The stepwise assembly process of the electrochemic...

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