Cell surface display of organophosphorus hydrolase for sensitive spectrophotometric detection of p-nitrophenol substituted organophosphates

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成果类型：

期刊论文

作者：

Tang, Xiangjiang;Liang, Bo;Yi, Tuyong;Manco, Giuseppe;Palchetti, Ilaria;...

通讯作者：

Liu, Aihua

作者机构：

[Tang, Xiangjiang; Liang, Bo; Liu, Aihua] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China.

[Tang, Xiangjiang; Liang, Bo; Liu, Aihua] Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China.

[Tang, Xiangjiang; Liu, Aihua] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.

[Yi, Tuyong] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Pests, Changsha 410128, Hunan, Peoples R China.

[Manco, Giuseppe] Italian Natl Res Council, Inst Prot Biochem, I-80131 Naples, Italy.

通讯机构：

[Liu, Aihua] C

Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China.

语种：

英文

关键词：

Bacterial surface display;Ice nucleation protein;Organophosphorus hydrolase;Spectrophotometric detection of organophosphates;p-Nitrophenol

期刊：

Enzyme and Microbial Technology

ISSN：

0141-0229

年：

2014

卷：

页码：

107-112

DOI：

10.1016/j.enzmictec.2013.10.006

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91227116, 31200598, 21275152]; Hundred-Talent-ProjectChinese Academy of Sciences [KSCX2-YW-BR-7]; Knowledge Innovation Project in Biotechnology, Chinese Academy of Sciences [KSCX2-EW-J-10-6]

机构署名：

本校为其他机构

院系归属：

植物保护学院

摘要：

Organophosphates (OPs) widely exist in ecosystem as toxic substances, for which sensitive and rapid analytical methods are highly requested. In the present work, by using N-terminal of ice nucleation protein (INP) as anchoring motif, a genetically engineered Escherichia coli (E. coli) strain surface displayed mutant organophosphorus hydrolase (OPH) (S5) with improved enzyme activity was successfully constructed. The surface location of INP-OPH fusion was confirmed by SDS-PAGE analysis and enzyme activity assays. The OPH-displayed bacteria facil...

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Cell surface display of organophosphorus hydrolase for sensitive spectrophotometric detection of p-nitrophenol substituted organophosphates

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