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Selection of reference genes for quantitative real-time RT-PCR analysis in citrus

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成果类型:
期刊论文
作者:
Yan, Jiawen;Yuan, Feirong;Long, Guiyou;Qin, Lei;Deng, Ziniu*
通讯作者:
Deng, Ziniu
作者机构:
[Deng, Ziniu; Yuan, Feirong; Long, Guiyou; Qin, Lei; Yan, Jiawen] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.
[Deng, Ziniu; Yuan, Feirong; Long, Guiyou; Yan, Jiawen] Natl Ctr Citrus Improvement, Changsha 410128, Hunan, Peoples R China.
[Deng, Ziniu; Yuan, Feirong; Yan, Jiawen] Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Deng, Ziniu] H
Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Citrus;Reference genes;qPCR;Gene expression
期刊:
Molecular Biology Reports
ISSN:
0301-4851
年:
2012
卷:
39
期:
2
页码:
1831-1838
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30871702]; China Modern Agricultural Research System [CARS-27]
机构署名:
本校为第一且通讯机构
院系归属:
园艺园林学院
摘要:
Quantitative real-time reverse transcription polymerase chain reaction (qPCR) has become the preferred method for studying low-abundant mRNA expression. Appropriate application of qPCR in such studies requires the use of reference gene(s) as an internal control in order to normalize the mRNA levels between different samples for an exact comparison of gene expression levels. Expression of the reference gene should be independent from development stage, cell/tissue types, treatments and environmental conditions. Recognizing the importance of reference gene(s) in normalization of qPCR data, vario...

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