The enteric septicemia of channel catfish, caused by Edwardsiella ictaluri, is one of the most severe diseases in channel catfish worldwide. Currently, a safe and highly effective vaccine is urgently needed for the control of this disease. In this study, E. ictaluri ghosts (EIGs) were first generated by PhiX174 lysis gene E, and the hematological indices and relative percent of survival (RPS) were examined in channel catfish (Ictalurus punctatus) after immunization. Generation of ghosts in the transformant of E. ictaluri carrying the plasmid pB...