期刊论文

Wu, Guoyao

英文

Amino Acids

0939-4451

2009

37

1

143-152

10.1007/s00726-009-0268-1

This work was supported by grants from the National Research Initiative Competitive Grant of the USDA Cooperative State Research, Education, and Extension Service (#2008-35206-18764), Texas AgriLife Research (#H-8200), National Natural Science Foundation of China (#30528006 and 30600434 and 30871801), the Program for Innovative Research Groups of Hubei Provincial Natural Science Foundation (#2007ABC009), and The Chinese Academy of Sciences (#2005-1-4).

本校为其他机构

This study was conducted using the piglet model to test the hypothesis that mucosal cells of the neonatal small intestine can degrade nutritionally essential amino acids (EAA). Enterocytes were isolated from the jejunum of 0-, 7-, 14-, and 21-day-old pigs, and incubated for 45 min in Krebs buffer containing plasma concentrations of amino acids and one of the following L-[1-14C]- or L-[U-14C]-amino acids plus unlabeled tracees at 0.5, 2, or 5 mM: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine....