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De Novo Transcriptome of Brassica juncea Seed Coat and Identification of Genes for the Biosynthesis of Flavonoids

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成果类型:
期刊论文
作者:
Liu, Xianjun;Lu, Ying;Yuan, Yuhui;Liu, Shuyan;Guan, Chunyun;...
通讯作者:
Liu, Zhongsong
作者机构:
[Guan, Chunyun; Lu, Ying; Liu, Zhongsong; Liu, Xianjun; Liu, Shuyan; Chen, Sheyuan; Yuan, Yuhui] Hunan Agr Univ, Oilseed Crops Inst, Changsha, Hunan, Peoples R China.
通讯机构:
[Liu, Zhongsong] H
Hunan Agr Univ, Oilseed Crops Inst, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
Brassica;Seed coat;Biosynthesis;Transcriptome analysis;Seeds;Sequence alignment;Sequence assembly tools;RNA sequencing
期刊:
PLoS ONE
ISSN:
1932-6203
年:
2013
卷:
8
期:
8
页码:
e71110-
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31101176, 30971799, 31271762]
机构署名:
本校为第一且通讯机构
院系归属:
农学院
摘要:
Brassica juncea, a worldwide cultivated crop plant, produces seeds of different colors. Seed pigmentation is due to the deposition in endothelial cells of proanthocyanidins (PAs), end products from a branch of flavonoid biosynthetic pathway. To elucidate the gene regulatory network of seed pigmentation in B. juncea, transcriptomes in seed coat of a yellow-seeded inbred line and its brown-seeded near- isogenic line were sequenced using the next-generation sequencing platform Illumina/Solexa and de novo assembled. Over 116 million high-quality reads were assembled into 69,605 unigenes, of which ...

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