Exploring Valid Reference Genes for Quantitative Real-time PCR Analysis in Plutella xylostella (Lepidoptera: Plutellidae)

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成果类型：

期刊论文

作者：

Fu, Wei;Xie, Wen;Zhang, Zhuo;Wang, Shaoli;Wu, Qingjun;...

通讯作者：

Zhou, Xuguo

作者机构：

[Zhou, Xiaomao; Fu, Wei] Hunan Agr Univ, Inst Pesticide Sci, Changsha 410128, Hunan, Peoples R China.

[Xie, Wen; Zhang, Youjun; Wang, Shaoli; Wu, Qingjun; Fu, Wei] Chinese Acad Agr Sci, Dept Plant Protect, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.

[Zhang, Zhuo; Liu, Yong; Fu, Wei] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China.

[Zhang, Zhuo; Liu, Yong] Key Lab Integrated Management Pests & Dis Hort Cr, Changsha 410125, Hunan, Peoples R China.

[Zhou, Xuguo] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.

通讯机构：

[Zhou, Xuguo] U

Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.

语种：

英文

关键词：

Plutella xylostella;abiotic factor.;biotic factor;qRT-PCR analysis;reference gene

期刊：

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES

ISSN：

1449-2288

年：

2013

卷：

期：

页码：

792-802

DOI：

10.7150/ijbs.5862

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071709, 31171876]; 863 ProgramNational High Technology Research and Development Program of China [2012AA101502]; Special Fund for Agro-scientific Research in the Public Interest [201103021]; Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables

机构署名：

本校为第一机构

院系归属：

植物保护学院

摘要：

Quantitative real-time PCR (qRT-PCR), a primary tool in gene expression analysis, requires an appropriate normalization strategy to control for variation among samples. The best option is to compare the mRNA level of a target gene with that of reference gene(s) whose expression level is stable across various experimental conditions. In this study, expression profiles of eight candidate reference genes from the diamondback moth, Plutella xylostella, were evaluated under diverse experimental conditions. RefFinder, a web-based analysis tool, integ...

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Exploring Valid Reference Genes for Quantitative Real-time PCR Analysis in Plutella xylostella (Lepidoptera: Plutellidae)

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