Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa

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成果类型：

期刊论文

作者：

Zeng, Jianguo;Liu, Yisong;Liu, Wei;Liu, Xiubing;Liu, Fuqing;...

通讯作者：

Guo, An-Yuan

作者机构：

[Liu, Xiubing; Liu, Yisong; Chen, Jinjun; Zeng, Jianguo; Xiong, Xingyao; Zhu, Pengcheng] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha, Hunan, Peoples R China.

[Liu, Xiubing; Liu, Yisong; Chen, Jinjun; Zeng, Jianguo; Xiong, Xingyao; Zhu, Pengcheng] Hunan Agr Univ, Natl Chinese Med Herbs Hunan Technol Ctr, Changsha, Hunan, Peoples R China.

[Zhang, Hong-Mei; Gong, Jing; Liu, Wei; Guo, An-Yuan; Liao, Yifang] Huazhong Univ Sci & Technol, Dept Biomed Engn, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.

[Wang, Depeng; Zhang, Hong-Mei; Gong, Jing; Liu, Wei; Liao, Yifang] Next Biosci Co Ltd, Wuhan, Peoples R China.

[Shi, Mingming; Guo, Fang] BGI Shenzhen, Shenzhen, Peoples R China.

通讯机构：

[Guo, An-Yuan] H

Huazhong Univ Sci & Technol, Dept Biomed Engn, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.

语种：

英文

关键词：

Alkaloids;Biosynthesis;Leaves;Gene expression;Transcriptome analysis;Fruits;Proteomes;Metabolic pathways

期刊：

PLOS ONE

ISSN：

1932-6203

年：

2013

卷：

期：

页码：

e53409-

DOI：

10.1371/journal.pone.0053409

基金类别：

China-Belgium Cooperation Research on Plants Medicine Utilization & Crop Pests Forecasting and Control Technology [2010DFA32810]; National Key technology R&D Program for demonstration of neotype Chinese herbal veterinary drugsNational Key Technology R&D Program [2011BAD34B02]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31171271]; Young Teachers' Fund for Doctor Stations; Ministry of Education of ChinaMinistry of Education, China [20110142120042]

机构署名：

本校为第一机构

院系归属：

园艺园林学院

摘要：

BACKGROUND: The Macleaya spp., including Macleaya cordata and Macleaya microcarpa, are traditional anti-virus, inflammation eliminating, and insecticide herb medicines for their isoquinoline alkaloids. They are also known as the basis of the popular natural animal food addictive in Europe. However, few studies especially at genomics level were conducted on them. Hence, we performed the Macleaya spp. transcriptome and integrated it with iTRAQ proteome analysis in order to identify potential genes involved in alkaloids biosynthesis. METHODOLOGY AND PRINCIPAL FINDINGS: We elaborately designed the...

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Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa

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