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Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation.

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WOS被引频次:188
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成果类型:
期刊论文
作者:
Wang, Junjun;Chen, Lixiang;Li, Peng;Li, Xilong;Zhou, Huaijun;Wang, Fenglai;Li, Defa;Yin, Yulong;Wu, Guoyao
通讯作者:
Wang, FL
作者机构:
[Chen, Lixiang; Li, Xilong; Wang, Junjun; Zhou, Huaijun; Li, Peng; Yin, Yulong] Texas A&M Univ Syst, Texas Agr Expt Stn, College Stn, TX 77843 USA.
[Chen, Lixiang] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
[Wang, Junjun; Wang, Fenglai; Wu, Guoyao; Li, Defa; Yin, Yulong] China Agr Univ, State Key Lab Anim Nutr, Beijing 100094, Peoples R China.
[Wu, Guoyao; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wang, Fenglai] China Agr Univ, State Key Lab Anim Nutr, Beijing 100094, Peoples R China.
语种:
英文
期刊:
The Journal of nutrition
ISSN:
0022-3166
年:
2008
卷:
138
期:
6
页码:
1025-1032
文献类别:
WOS:Article
所属学科:
ESI学科类别:农业科学;WOS学科类别:Nutrition & Dietetics
入藏号:
WOS:000255920900007;PMID:18492829
机构署名:
本校为其他机构
院系归属:
动物科学技术学院
摘要:
Dietary supplementation of glutamine prevents intestinal dysfunction and atrophy in weanling piglets, but the underlying mechanism(s) are largely unknown. This study was conducted to test the hypothesis that weaning or glutamine may modulate expression of genes that are crucial for intestinal metabolism and function. In Expt. 1, we obtained small intestine from 28-d-old pigs weaned at 21 d of age and from age-matched suckling piglets. In Expt. 2, piglets were weaned at 21 d of age and then had free access to diets supplemented with 1% L-glutamine (wt:wt) or isonitrogenous L-alanine (control). At d 28, we collected small intestine for biochemical and morphological measurements and microarray analysis of gene expression using the Operon Porcine Genome Oligo set. Early weaning resulted in increased (52-346%) expression of genes related to oxidative stress and immune activation but decreased (35-77%) expression of genes related to macronutrient metabolism and cell proliferation in the gut. Dietary glutamine supplementation increased intestinal expression (120-124%) of genes that are necessary for cell growth and removal of oxidants, while reducing (34-75%) expression of genes that promote oxidative stress and immune activation. Functionally, the glutamine treatment enhanced intestinal oxidative-defense capacity (indicated by a 29% increase in glutathione concentration), prevented jejunal atrophy, and promoted small intestine growth (+12%) and body weight gain (+19%) in weaned piglets. These findings reveal coordinate alterations of gene expression in response to weaning and aid in providing molecular mechanisms for the beneficial effect of dietary glutamine supplementation to improve nutrition status in young mammals.
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