Characterization of homologous and heterologous interactions between viroplasm proteins P6 and P9-1 of the fijivirus southern rice black-streaked dwarf virus
National Science and Technology Support Program [2012BAD19B03]; China 973 ProgramNational Basic Research Program of China [2010CB126203]; Special Fund for Agro-Scientific Research in the Public Interest of China [201003031]; 863 ProgramNational High Technology Research and Development Program of China [2007AA10Z414]; Zhejiang Provincial Science and Technology Project [2010C12027]; Zhejiang Provincial Foundation for Natural ScienceNatural Science Foundation of Zhejiang Province [LQ14C140003]
机构署名：
本校为其他机构
院系归属：
植物保护学院
摘要：
P6 of southern rice black-streaked dwarf virus (SRBSDV) is a multifunctional protein that is involved in the formation of viroplasms by interacting with P5-1. Here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between SRBSDV P6 and P9-1 in yeast and plant cells. Mutational analysis showed that the N-terminal region (residues 1-93) of P6 was necessary for the interaction between P6 and P9-1. Self-interactions only occurred between the full-length P6 or P9-1. P9-1 was able to form viroplasm-like incl...
