Improving 2-Phenylethanol production via Ehrlich pathway using genetic engineered saccharomyces cerevisiae strains

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成果类型：

期刊论文

作者：

Yin, Sheng;Zhou, Hui;Xiao, Xiao;Lang, Tiandan;Liang, Jingru;...

通讯作者：

Wang, Chengtao

作者机构：

[Liang, Jingru; Xiao, Xiao; Wang, Chengtao; Yin, Sheng; Lang, Tiandan] Beijing Technol & Business Univ, Sch Food & Chem Engn, Beijing, Peoples R China.

[Wang, Chengtao; Yin, Sheng; Lang, Tiandan] Beijing Technol & Business Univ, Beijing Engn & Technol Res Ctr Food Addit, Beijing, Peoples R China.

[Xiao, Xiao; Wang, Chengtao; Yin, Sheng] Beijing Technol & Business Univ, Beijing Lab Food Qual & Safety, Beijing, Peoples R China.

[Liang, Jingru; Wang, Chengtao; Yin, Sheng] Beijing Technol & Business Univ, Beijing Key Lab Flavor Chem, Beijing 100048, Peoples R China.

[Zhou, Hui] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.

通讯机构：

[Wang, Chengtao] B

Beijing Technol & Business Univ, Beijing Key Lab Flavor Chem, 11 Fu Cheng Rd, Beijing 100048, Peoples R China.

语种：

英文

关键词：

Recombinant Strain;Wild Strain;Aminotransferase Activity;Decarboxylase Activity;Yeast Extract Peptone Dextrose

期刊：

Current Microbiology

ISSN：

0343-8651

年：

2015

卷：

期：

页码：

762-767

DOI：

10.1007/s00284-015-0785-y

基金类别：

Beijing Municipal Natural Science FoundationBeijing Natural Science Foundation [5122008]; National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31071593]; Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality [IDHT20130506]

机构署名：

本校为其他机构

院系归属：

食品科学技术学院

摘要：

2-phenylethanol (2-PE) is an important aromatic compound with a rose-like fragrance widely used in food industry and cosmetic manufacture. In order to obtain “natural” 2-PE, the genetically modified budding yeasts were developed and applied for the 2-PE production. The gene ARO8 encoding transaminase and the gene ARO10 encoding decarboxylase in the Ehrlich pathway were expressed in Saccharomyces cerevisiae S288c. The activities of transaminase and decarboxylase were both enhanced in the corresponding recombinant strains. Consequently, the 2-P...

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Improving 2-Phenylethanol production via Ehrlich pathway using genetic engineered saccharomyces cerevisiae strains

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