期刊论文

Construction of mutant library and research on phenotypic variation of mutant population in the pepper(Capsicum annuum L.)

中文

湖南农业大学学报. 自然科学版

Journal of Hunan Agricultural University(Natural Sciences)

1007-1032

2017

43

1

31-36

10.13331/j.cnki.jhau.2017.01.006

湖南省自然科学基金项目(2016JJ2076)； 农业部产业技术体系项目(CARS–25–A–8)；

本校为第一机构

选用辣椒自交系‘6421'作为构建突变体库的试验材料,分析甲基磺酸乙酯(EMS)处理对辣椒种子萌发和幼苗生理特性的影响,确定适宜的EMS诱变浓度,在此基础上构建辣椒突变体库,并对突变体库中突变群体的表型变异进行分析。结果表明:EMS质量分数为1.0%时,辣椒种子发芽率和幼苗成活率分别为41.01%和49.32%,接近半致死剂量,且该浓度下辣椒幼苗中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均达最大值,分别为246.45 U/g、33.99 U/g和158.10 U/(g·min),丙二醛(MDA)含量为对照的1.85倍;初步构建了含有562个家系辣椒突变体库,突变类型可归纳为叶、茎秆、果实、育性、生育期等5个类别,突变植株数分别占总突变植株数的12.73%、21.56%、6.92%、45.33%和13.46%。

In this study,the inbred line‘6421’was selected and treated with different concentrations of mutagen(ethyl methane sulfonate(EMS))to study the effect of EMS treatments on seeds germination and some biochemicalcharacteristics of seedlings,to construct mutant library and analyze the phenotypic traits.The results showed that treatedwith1.0%EMS,the germination rate and seedling survival rate were41.01%and49.32%,respectively,which were closeto those with LD50,the seedlings had the highest enzyme activities of SOD(246.45U/g),POD(33.99U/g)and CAT(158.10U/(g·min)),and the MDA content of seedlings was1.85times as much as the control.562mutant lines wereidentified and classified into five categories:leaf,stem,fruit,fertility and growth period mutations,which wereaccounting for12.73%,21.56%,6.92%,45.33%and13.46%of the total mutation respectively.