摘要：
The invention discloses a method for cyclically degrading nitrobenzene in wastewater by utilizing extracellular polymeric substances to reinforce nano zero-valent iron. The method comprises the following steps of adjusting the pH value of the nitrobenzene-containing wastewater to 6.5-7.5, adding nano zero-valent iron and an extracellular polymeric substance, carrying out a stirring reaction, adding the nitrobenzene-containing wastewater again, adjusting the pH value to 6.5-7.5, adding the nano zero-valent iron and the extracellular polymeric substance, carrying out a stirring reaction, and repeating the above steps to realize cyclic degradation. According to the method, precipitation and aggregation of the nano zero-valent iron particles can be reduced through the extracellular polymeric substance, ferric oxide on the surfaces of the nano zero-valent iron particles is reduced, surface active sites are increased, the efficiency in the nitrobenzene cyclic degradation process is improved,the service life of the nano zero-valent iron is prolonged, and the method is easy to operate and suitable for industrial application.
摘要：
The invention relates to an absolute quantitative PCR method for rapidly determining the titer of ascoviruses. The absolute quantitative PCR method comprises the following steps: ultrasonically crushing a to-be-detected sample containing the ascoviruses, carrying out diluting and filtering, allowing the ascoviruses to infect cells in a logarithmic phase for 1 hour, collecting a cell suspension, calculating the number of the cells, and extracting total DNA; carrying out qPCR detection on the DNA, and drawing a standard curve of absolute quantitative PCR detection by taking a pGEM-T vector containing the fragment of the DNA as a standard substance; calculating the copy number of a virus genome contained in each sample according to the Ct value of each sample; and according to an infection complex number formula, calculating the average number of virus particles infecting each cell, namely the ratio of the number of viruses to the number of the cells during infection so as to calculate the titer of the viruses. The absolute quantitative PCR method provided by the invention is used for measuring the titer of the ascoviruses; the detection time is only 4-6 hours when the concentration of a sample is 1 * 10 to 1 * 10 virus gene copy/mL, so the consumed time is short; compared with a traditional virus titer detection method, the absolute quantitative PCR method provided by theinvention has simple and rapid operation, is accurate, greatly improves the detection efficiency of the titer of the ascoviruses, and is especially applicable to rapid determination of the titer of the ascoviruses.
