作者机构：
[Chen, Yinke; Peng, Yan; Teng, Zhenning; Duan, Meijuan; Ye, Nenghui; Qin, Zhonge; Liu, Bohan; Yu, Huihui; Ye, NH; Meng, Shuan; Lv, Jiahan; Duan, MJ] Hunan Agr Univ, Coll Agron, Hunan Prov Key Lab Rice Stress Biol, Changsha 410128, Peoples R China.;[Teng, Zhenning; Zhang, Jianhua; Yu, Huihui] Chinese Univ Hong Kong, Sch Life Sci, Hong Kong 999077, Peoples R China.;[Teng, Zhenning; Zhang, Jianhua; Yu, Huihui] Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong 999077, Peoples R China.;[He, YC; He, Yuchi] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430000, Peoples R China.;[Zhang, Jianhua; Ye, Nenghui; Ye, NH] Hong Kong Baptist Univ, Dept Biol, Hong Kong 999077, Peoples R China.
通讯机构：
[He, YC ; Zhang, JH; Ye, NH ; Duan, MJ] H;[Zhang, JH ] C;Hunan Agr Univ, Coll Agron, Hunan Prov Key Lab Rice Stress Biol, Changsha 410128, Peoples R China.;Chinese Univ Hong Kong, Sch Life Sci, Hong Kong 999077, Peoples R China.;Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong 999077, Peoples R China.
摘要：
Low-temperature germination (LTG) is an important agronomic trait for direct-seeding cultivation of rice (Oryza sativa). Both OsMYB30 and OsTPP1 regulate the cold stress response in rice, but the function of OsMYB30 and OsTPP1 in regulating LTG and the underlying molecular mechanism remains unknown. Employing transcriptomics and functional studies revealed a sugar signaling pathway that regulates seed germination in response to low temperature (LT). Expression of OsMYB30 and OsTPP1 was induced by LT during seed germination, and overexpressing either OsMYB30 or OsTPP1 delayed seed germination and increased sensitivity to LT during seed germination. Transcriptomics and qPCR revealed that expression of OsTPP1 was upregulated in OsMYB30-overexpressing lines but downregulated in OsMYB30-knockout lines. In vitro and in vivo experiments revealed that OsMYB30 bound to the promoter of OsTPP1 and regulated the abundance of OsTPP1 transcripts. Overaccumulation of trehalose (Tre) was found in both OsMYB30- and OsTPP1-overexpressing lines, resulting in inhibition of alpha-amylase 1a (OsAMY1a) gene during seed germination. Both LT and exogenous Tre treatments suppressed the expression of OsAMY1a, and the osamy1a mutant was not sensitive to exogenous Tre during seed germination. Overall, we concluded that OsMYB30 expression was induced by LT to activate the expression of OsTPP1 and increase Tre content, which thus inhibited alpha-amylase activity and seed germination. This study identified a phytohormone-independent pathway that integrates environmental cues with internal factors to control seed germination. Low temperature increases the abundance of a transcription factor, which activates the biosynthesis of trehalose and consequently inhibits seed germination by impeding alpha-amylase activity in rice.
摘要：
Effects of steam explosion (SE) treatment (0.3-1.0 MPa, 30-90s) on the physicochemical and functional properties of dietary fiber (DF) extracted from tea residue were investigated. Results showed that SE led to the formation of porous and less compact microstructures of tea residues. Compared with the control sample (12.41 g/100 g), the content of soluble dietary fiber (SDF) was markedly increased by SE, reaching the maximum of 23.03 g/100 g when steam explored at 0.5 MPa for 90 s, due to the degradation of insoluble dietary fiber (IDF). The physicochemical and functional properties were significantly influenced by SE treatments. IDF exhibited decreased water holding capacity (WHC), swelling capacity (SC), oil holding capacity (OHC) after SE, whereas the physicochemical properties of SDF from SE-treated tea residues (0.3 MPa, 90s) were greatly improved. The glucose absorption capacity (GAC), cholesterol adsorption capacity (CAC), and nitrite ion adsorption capacity (NIAC) of both IDF and SDF showed trends of first increasing and then decreasing, indicating that excessive SE treatment resulted in poor properties of DF. These findings are of great value for the high-value utilization of agricultural by-product and development of functional foods.
摘要：
Cadmium (Cd) is a highly toxic heavy metal that can be readily absorbed by plants and enriched in human body. Rice (Oryza sativa L.) yield and grain quality are affected by excessive Cd in the soil. Therefore, understanding the mechanisms of Cd absorption, accumulation and detoxification in the root apex is crucial for developing low-Cd rice cultivars. After Cd treatment, Cd concentration in rice root tips (RT) was 1.4 times higher than that in basal roots (BR). To uncover the distinct molecular responses to Cd toxicity, we conducted transcriptomic, proteomic, and metabolomic analyses on the two root sections. The results revealed that the RT exhibited 1.2-2.0 fold higher transcript or protein abundance of several Cd-related transporters than the BR, including Nramp1, Nramp5, IRT1, and HMA3, thereby contributing to more Cd accumulation in the RT. Furthermore, multi-omics analysis unveiled that the RT had enhanced activity in 'phenylpropanoid metabolism', 'AsA-GSH cycle' and 'tryptophan metabolism', conferring the stronger antioxidant system. While the BR showed higher activation in 'cell wall remodeling' and 'terpenoid biosynthesis'. This comprehensive study provides insights into the regulatory network of genes, proteins and metabolites involved in the differential responses to Cd toxicity between rice root tips and mature zones.
摘要：
The species and contents of a-dicarbonyls in commercial black tea were examined, along with the effects of the manufacturing process and drying temperature on the formation of a-dicarbonyls. Ten a-dicarbonyls were quantified in commercial and in -process black tea samples by using UPLC-MS/MS and their derived quinoxalines. The a-dicarbonyls content in commercial black tea decreased significantly (p < 0.05) in the following order: 3deoxyglucosone > glucosone > 3-deoxypentosone = threosone > galactosone >= methylglyoxal = glyoxal >= 3deoxygalactosone = 3-deoxythreosone = diacetyl. Except for 3-deoxyglucosone and 3-deoxygalactosone, a further eight a-dicarbonyls were identified in all manufacturing steps of black tea. Except for the drying step, the rolling and fermenting played important roles in the formation of a-dicarbonyls. The total contents of a-dicarbonyls in black tea infusion ranged from 16.48 to 75.32 mu g/g based on our detected ten a-dicarbonyls.
作者机构：
[Le Huang; Huimin Zhang; Jiayi Tang; Na Li; Alessandra Gentile; Haojie Yin; Suming Dai; Yan Jin; Xiaopeng Lu; Hongbin Liu; Chouyu Miao] National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China;[Alessandra Gentile] Department of Agriculture and Food Science, University of Catania, Catania, 95123, Italy;[Ling Zhang] Agriculture and Rural Bureau of Mayang Miao Autonomous County, Huaihua, China;[Ling Sheng] National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China. shengling0629@163.com
通讯机构：
[Ling Sheng] N;National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, China
摘要：
Anthocyanins are the most important compounds for nutritional quality and economic values of blood orange. However, there are few reports on the pre-harvest treatment accelerating the accumulation of anthocyanins in postharvest blood orange fruit. Here, we performed a comparative transcriptome and metabolomics analysis to elucidate the underlying mechanism involved in seasonal drought (SD) treatment during the fruit expansion stage on anthocyanin accumulation in postharvest ‘Tarocco’ blood orange fruit. Our results showed that SD treatment slowed down the fruit enlargement and increased the sugar accumulation during the fruit development and maturation period. Obviously, under SD treatment, the accumulation of anthocyanin in blood orange fruit during postharvest storage was significantly accelerated and markedly higher than that in CK. Meanwhile, the total flavonoids and phenols content and antioxidant activity in SD treatment fruits were also sensibly increased during postharvest storage. Based on metabolome analysis, we found that substrates required for anthocyanin biosynthesis, such as amino acids and their derivatives, and phenolic acids, had significantly accumulated and were higher in SD treated mature fruits compared with that of CK. Furthermore, according to the results of the transcriptome data and weighted gene coexpression correlation network analysis (WGCNA) analysis, phenylalanine ammonia-lyase (PAL3) was considered a key structural gene. The qRT-PCR analysis verified that the PAL3 was highly expressed in SD treated postharvest stored fruits, and was significantly positively correlated with the anthocyanin content. Moreover, we found that other structural genes in the anthocyanin biosynthesis pathway were also upregulated under SD treatment, as evidenced by transcriptome data and qRT-PCR analysis. The findings suggest that SD treatment promotes the accumulation of substrates necessary for anthocyanin biosynthesis during the fruit ripening process, and activates the expression of anthocyanin biosynthesis pathway genes during the postharvest storage period. This is especially true for PAL3, which co-contributed to the rapid accumulation of anthocyanin. The present study provides a theoretical basis for the postharvest quality control and water-saving utilization of blood orange fruit.
摘要：
BACKGROUND: Dichlorvos (DDVP), as a highly effective insecticide, is widely used in agricultural production. However, DDVP residue in foodstuffs adversely affects human health. Conventional instrumental analysis can provide highly sensitive and accurate detection of DDVP, while the need of bulky and expensive equipment limits their application in resource-poor areas and on-site detection. Therefore, the development of easily portable sensing platforms for convenient, rapid and sensitive quantification of DDVP is very essential for ensuring food safety. RESULT: A portable colorimetric sensing platform for rapid and sensitive quantification of DDVP is developed based on nanozyme-participated highly efficient chromogenic catalysis. The Fe-Mn bimetallic oxide (FeMnOx) nanozyme possesses excellently oxidase-like activity and can efficiently catalyze oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB) into a blue oxide with a very low Michaelis constant (K(m)) of 0.0522mM. The nanozyme-catalyzed chromogenic reaction can be mediated by DDVP via inhibiting the acetylcholinesterase (AChE) activity. Thus, trace DDVP concentration-dependent color evolution is achieved and DDVP can be sensitively detected by spectrophotometry. Furthermore, a smartphone-integrated 3D-printed miniature lightbox is fabricated as the colorimetric signal acquisition and processing device. Based on the FeMnOx nanozyme and smartphone-integrated lightbox system, the portable colorimetric sensing platform of DDVP is obtained and it has a wide linear range from 1 to 3000ngmL(-1) with a low limit of detection (LOD) of 0.267ngmL(-1) for DDVP quantification. SIGNIFICANCE: This represents a new portable colorimetric sensing platform that can perform detection of DDVP in foodstuffs with simplicity, sensitivity, and low cost. The work not only offers an alternative to rapid and sensitive detection of DDVP, but also provides a new insight for the development of advanced sensors by the combination of nanozyme, 3D-printing and information technologies.
期刊：
Frontiers in Microbiology,2024年15:1374199 ISSN：1664-302X
作者机构：
College of Environment and Ecology of Hunan Agricultural University, China;Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, China
摘要：
The combined application of chemical and organic fertilizers has been recognized to enhance soil fertility and foster the soil microbial ecosystem. However, the optimal ratio of chemical and organic fertilizers in oilseed rape cultivation is still uncertain, and the role of rhizosphere effect is still unclear. Thus, this study aimed to elucidate the impacts of varying ratios of chemical and organic fertilizers on the structure and potential functionalities of rhizosphere and non-rhizosphere soil microbial communities. The interplay of microbial communities with soil properties and oilseed rape root exudates was investigated in controlled pot cultivations receiving varying ratios of chemical and organic fertilizers. Results indicated clear segregation in the soil bacterial community, influenced by both fertilization treatments and rhizosphere effects. The bacterial community structure significantly correlated with nitrate nitrogen, organic acids, and dissolved organic carbon (DOC) content. Rhizosphere effects led to increased bacteria abundance, reduced diversity, and decreased network stability. Notably, F3 treatment receiving 25% chemical and 75% organic fertilizers showed a significantly higher abundance at 1.43 × 1011 copies g−1 dry soil, accompanied by increased species and genetic diversity, and ecological network complexity. This treatment also yielded the highest aboveground biomass of oilseed rape. However, the application of organic fertilizers also increased the risk of plant pathogenicity. This study reveals the impact of fertilizers and rhizosphere effects on soil microbial community structure and function, shedding light on the establishment of more effective fertilization schemes for oilseed rape agriculture.
通讯作者：
Xin Long Xu<&wdkj&>Xin Long Xu Xin Long Xu Xin Long Xu
作者机构：
[Liu, Shun Jia] Hunan Agr Univ, Sch Business, Changsha, Peoples R China.;[Zhu, Xiaoqian; Li, Jianping] Univ Chinese Acad Sci, Sch Econ & Management, Beijing, Peoples R China.;[Zhu, Xiaoqian; Li, Jianping] UCAS, MOE Social Sci Lab Digital Econ Forecasts & Policy, Beijing, Peoples R China.;[Wu, Dengsheng] Chinese Acad Sci, Inst Sci & Dev, Beijing, Peoples R China.;[Xu, Xin Long] Hunan Normal Univ, Coll Tourism, Changsha, Peoples R China.
通讯机构：
[Xin Long Xu; Xin Long Xu Xin Long Xu Xin Long Xu] C;College of Tourism, Hunan Normal University, Changsha, China<&wdkj&>Institute of Interdisciplinary Studies, Hunan Normal University, Changsha, China
摘要：
Existing studies on the environmental Kuznets curve (EKC) neglect the inverse effect of pollution transfer from environmental regulation interactions on pollution reduction from a risk analysis perspective. Based on the regional differentiated attitudes on the environmental regulation reached in risk communication by the risk awareness biases of multiple interest groups, this article clarifies the causality between risk communication and risk transfer based on multistakeholder engagement processes; furthermore, the article incorporates the simultaneous action of the technological innovation effect and pollution risk transfer effect to construct a spatial environmental hyperbolic model with a bidirectional correlation between pollution emissions and economic growth in different regions. To verify our model, we select the pollution from agricultural watersheds in China as a sample to examine the two inverse effects. The results demonstrate that (1) agricultural watershed pollution and economic growth show an inverted U-shaped relation and a U-shaped relation in the local region and adjacent regions, respectively; (2) the pollution reduction assessment of the classical EKC model can be largely attributed to pollution risk transfer behavior; and (3) the turning point of the U-shaped curve appears earlier than that of the inverted U-shaped curve in the spatial hyperbola model. The findings suggest that stakeholders should consider the risk awareness bias caused by the imbalance of regional economic development and the scenarios that provide a "haven" for pollution risk transfer. Moreover, our study expands the theoretical connotation of the classical EKC hypothesis and is more suitable for pollution reduction scenarios in developing countries.
期刊：
FRONTIERS IN PLANT SCIENCE,2024年15:1364826 ISSN：1664-462X
作者机构：
[Xue, Shuai; Xiao, Liang; Li, Shicheng; Yi, Zili; Tang, Yanmei] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China;[Zheng, Xuying; Zerpa-Catanho, Dessireé] Department of Crop Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, United States;[Zhang, Zhihai] Institute for Sustainability, Energy, and Environment, University of Illinois at Urbana-Champaign, Champaign, IL, United States;[Yang, Sai] Orient Science & Technology College of Hunan Agricultural University, Changsha, Hunan, China;[Kuang, Xianyan] Department of Biological and Environmental Sciences, Alabama A&M University, Huntsville, AL, United States
摘要：
Marginal lands, such as those with saline soils, have potential as alternative resources for cultivating dedicated biomass crops used in the production of renewable energy and chemicals. Optimum utilization of marginal lands can not only alleviate the competition for arable land use with primary food crops, but also contribute to bioenergy products and soil improvement. Miscanthus sacchariflorus and M. lutarioriparius are prominent perennial plants suitable for sustainable bioenergy production in saline soils. However, their responses to salt stress remain largely unexplored. In this study, we utilized 318 genotypes of M. sacchariflorus and M. lutarioriparius to assess their salt tolerance levels under 150 mM NaCl using 14 traits, and subsequently established a mini-core elite collection for salt tolerance. Our results revealed substantial variation in salt tolerance among the evaluated genotypes. Salt-tolerant genotypes exhibited significantly lower Na(+) content, and K(+) content was positively correlated with Na(+) content. Interestingly, a few genotypes with higher Na(+) levels in shoots showed improved shoot growth characteristics. This observation suggests that M. sacchariflorus and M. lutarioriparius adapt to salt stress by regulating ion homeostasis, primarily through enhanced K(+) uptake, shoot Na(+) exclusion, and Na(+) sequestration in shoot vacuoles. To evaluate salt tolerance comprehensively, we developed an assessment value (D value) based on the membership function values of the 14 traits. We identified three highly salt-tolerant, 50 salt-tolerant, 127 moderately salt-tolerant, 117 salt-sensitive, and 21 highly salt-sensitive genotypes at the seedling stage by employing the D value. A mathematical evaluation model for salt tolerance was established for M. sacchariflorus and M. lutarioriparius at the seedling stage. Notably, the mini-core collection containing 64 genotypes developed using the Core Hunter algorithm effectively represented the overall variability of the entire collection. This mini-core collection serves as a valuable gene pool for future in-depth investigations of salt tolerance mechanisms in Miscanthus.
摘要：
Efforts to curtail the escalating health threat posed by methicillin-resistant Staphylococcus aureus (MRSA), a formidable superbug, necessitate the development of innovative treatment strategies. Leveraging potential compounds from natural sources in tandem with antibiotics has emerged as a promising approach against MRSA. These strategies should enhance the antibiotic efficacy, reduce dosage and toxicity, and bypass MRSA resistance. In this study, we used a checkerboard assay to illustrate the significant synergistic anti-MRSA effect of shikimic acid (SA), a naturally occurring compound, and ceftiofur (CF). Time-kill curves further revealed that a combination of 1/4 of the minimum inhibitory concentration (MIC) of SA and 1/8 MIC of the sodium CF eradicated MRSA within 2h, with no noticeable toxicity observed with these concentrations. In vivo experiments confirmed that this combination therapy demonstrated robust antimicrobial activity against MRSA-induced bacteremia in mice, significantly reducing bacterial loads in the kidneys, liver, and spleen, attenuating inflammatory cell infiltration, and alleviating pathological damage. This study not only offers a compelling strategy, capitalizing on the synergistic potential of SA and CF, to rapidly address antibiotic resistance but also contributes significantly to the refinement of antimicrobial therapeutic strategies.
摘要：
Unsupervised cross-modal hashing is a topic of considerable interest due to its advantages in terms of low storage costs and fast retrieval speed. Despite the impressive achievements of existing solutions, two challenges remain unaddressed: (1) Semantic similarity obtained without supervision is not accurate enough, and (2) the preservation of similarity structures lacks effectiveness due to the neglect of both global and local similarity. This paper introduces a new method, Multi-Grained Similarity Preserving and Updating (MGSPU), to tackle these challenges. To overcome the first challenge, MGSPU employs a newly designed strategy to update the semantic similarity matrix, effectively generating a high-confidence similarity matrix by eliminating noise in the original cross-modal features. For the second challenge, a novel multi-grained similarity preserving method is proposed, aiming to enhance cross-modal hash code learning by learning consistency in multi-grained similarity structures. Comprehensive experiments on two widely used datasets with nine state-of-the-art competitors validate the superior performance of our method in cross-modal hashing.
作者：
Yi Su;Xiaoyun Peng;Haiou Li;Wenzhen Ku;Ruozhong Wang;...
期刊：
Plants-Basel,2024年13(6):899- ISSN：2223-7747
通讯作者：
Haiou Li<&wdkj&>Langtao Xiao
作者机构：
Authors to whom correspondence should be addressed.;[Yi Su; Ruozhong Wang] Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China;Hunan Provincial Key Lab of Dark Tea and Jin-Hua, College of Materials and Chemical Engineering, Hunan City University, Yiyang 413000, China;[Xiaoyun Peng; Wenzhen Ku] Hunan Provincial Key Lab of Dark Tea and Jin-Hua, College of Materials and Chemical Engineering, Hunan City University, Yiyang 413000, China<&wdkj&>Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China;[Haiou Li; Langtao Xiao] Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Haiou Li; Langtao Xiao] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
摘要：
Axillary bud outgrowth, a key factor in ratoon rice yield formation, is regulated by several phytohormone signals. The regulatory mechanism of key genes underlying ratoon buds in response to phytohormones in ratoon rice has been less reported. In this study, GR24 (a strigolactone analogue) was used to analyze the ratooning characteristics in rice cultivar Huanghuazhan (HHZ). Results show that the elongation of the axillary buds in the first seasonal rice was significantly inhibited and the ratoon rate was reduced at most by up to 40% with GR24 treatment. Compared with the control, a significant reduction in the content of auxin and cytokinin in the second bud from the upper spike could be detected after GR24 treatment, especially 3 days after treatment. Transcriptome analysis suggested that there were at least 742 and 2877 differentially expressed genes (DEGs) within 6 h of GR24 treatment and 12 h of GR24 treatment, respectively. Further bioinformatics analysis revealed that GR24 treatment had a significant effect on the homeostasis and signal transduction of cytokinin and auxin. It is noteworthy that the gene expression levels of OsCKX1, OsCKX2, OsGH3.6, and OsGH3.8, which are involved in cytokinin or auxin metabolism, were enhanced by the 12 h GR24 treatment. Taken overall, this study showed the gene regulatory network of auxin and cytokinin homeostasis to be regulated by strigolactone in the axillary bud outgrowth of ratoon rice, which highlights the importance of these biological pathways in the regulation of axillary bud outgrowth in ratoon rice and would provide theoretical support for the molecular breeding of ratoon rice.
摘要：
Brassinosteroids (BRs) are a class of naturally occurring steroidal phytohormones mediating a wide range of pivotal developmental and physiological functions throughout plant's life cycle. Therefore, it is of great significance to determine the content and the distribution of BRs in plants.Regretfully, although a large number of quantitative methods for BRs by liquid chromatograph-mass spectrometry (LC-MS/MS) have been reported, the in planta distribution of BRs is still unclear because of their lower contents in plant tissues and the lack of effective ionizable groups in their chemical structures. In this study, we established a novel analytical method of BRs based on C18 cartridge solid-phase extraction (SPE) purification, 4-dimethylaminophenylboronic acid (DMAPBA) derivatization and online valve-switching system coupled with ultra-high performance liquid chromatography-electrospray ionization-triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS). This method has been used to quantify three structural types of BRs (epibrassinolide, epicastasterone and 6-deoxo-24-epicastasterone) in different organs of Brassica napus L. (rapeseed). We found that the contents of three BRs in plant 删除[赵畅宇妈妈]: flowers were the highest, followed by tender pods. The levels of three BRs all decreased during the maturation of the organs. Based on the organ-level distribution of BRs in rapeseed, the spatial distribution maps of three BRs in rapeseed were outlined for the first time. Our results provided useful information for the precise in-situ localization of BRs in plants and the metabolomic research of BRs in future work.
作者：
Li Kong;Xinyu Gao;Lijuan Zhu;Xing Lin;You Huang;...
期刊：
食品科学与人类健康,2024年13(3):1292-1302 ISSN：2213-4530
通讯作者：
Zhihang Yuan<&wdkj&>Jin’e Yi
作者机构：
[Li Kong; Xinyu Gao; Lijuan Zhu; Xing Lin; You Huang; Chunlin Huang; Wenjiang Yang; Yazhi Chen; Haoqiang Zhao; Jing Wu; Zhihang Yuan; Jin’e Yi] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Zhihang Yuan; Jin’e Yi] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
摘要：
F-2 toxin is an estrogenic mycotoxin that causes reproductive disorders in animals. Betulinic acid (BA) is a natural pentacyclic lupane-structure triterpenoid that has diverse pharmacological activities. In this study, the antioxidative and anti-inflammatory effects of BA and its underlying mechanism are explored in F-2 toxin-triggered mouse ovarian damage. We found that BA alleviated the F-2 toxin-induced ovarian impairment by stimulating follicle growth, reducing inflammatory cell infiltration, repairing damaged mitochondria and endoplasmic reticulum. Simultaneously, BA not only reversed F-2 toxin-induced reduction of follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels in the serum, but also restrained the protein expression of the estrogen receptors α (ERα) and ERβ. Moreover, BA restored the balance of F-2 toxin-induced ovarian redox system disorders. Subsequently, we found that 0.25 mg/kg BA played an anti-inflammatory role in the F-2 toxin-induced ovarian impairment by decreasing interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) mRNA expression, as well as inhibiting p38 protein expression. These data demonstrated that BA exerts its protective effect on F-2 toxin-induced ovarian oxidative impairment and inflammation by inhibiting p38 expression, which implies a natural product-based medicine to ameliorate F-2 toxin-caused female reproductive toxicity and provides a detoxifying method for food contaminated by mycotoxin.
