作者：
Wan, Chunpeng (Craig);Hu, Xiaomeng;Li, Mingxi;Rengasamy, Kannan R. R.;Cai, Yi;...
期刊：
Journal of Functional Foods,2023年104:105506 ISSN：1756-4646
通讯作者：
Li, MX;Cai, Y;Liu, ZH
作者机构：
[Li, Mingxi; Wan, Chunpeng (Craig)] Jiangxi Agr Univ, Coll Agron, Res Ctr Tea & Tea Culture, Nanchang 330045, Peoples R China.;[Hu, Xiaomeng] Yili Normal Univ, Univ & Coll Key Lab Nat Prod Chem & Applicat Xinji, Sch Chem & Environm Sci, Yining 835000, Peoples R China.;[Rengasamy, Kannan R. R.] Saveetha Inst Med & Tech Sci SIMATS, Saveetha Dent Coll, Dept Pharmacol, Lab Nat Prod & Med Chem LNPMC, Chennai 600077, India.;[Cai, Yi] Guangzhou Med Univ, Sch Pharmaceut Sci, Key Lab Mol Target & Clin Pharmacol, Guangzhou 511436, Peoples R China.;[Cai, Yi] Guangzhou Med Univ, Sch Pharmaceut Sci, State & NMPA Key Lab Resp Dis, Guangzhou 511436, Peoples R China.
通讯机构：
[Cai, Y ] G;[Li, MX ] J;[Liu, ZH ] H;Jiangxi Agr Univ, Coll Agron, Res Ctr Tea & Tea Culture, Nanchang 330045, Peoples R China.;Guangzhou Med Univ, Sch Pharmaceut Sci, Key Lab Mol Target & Clin Pharmacol, Guangzhou 511436, Peoples R China.
关键词：
Green tea;Dietary supplements;EGCG;Inflammation;Aging
摘要：
Green tea and its active extracts have been widely used in a variety of dietary supplements. However, to date, the interaction of these bioactive compounds in green tea and their protein targets are not well studied. We first used the bioinformatics analysis of the relevant diabetes-related targets with the green tea bioactive compounds target proteins to construct a protein interaction (PPI) network to screen the core targets. The results of in vitro experiments showed that EGCG, a major polyphenolic component of green tea, inhibited HG-induced cardiomyocyte hypertrophy, inflammation, oxidative stress response, and cellular senescence. It was found that the PPARG-dependent pathways were involved in the protective effects of EGCG against HG-induced myocardial injury and senescence. This study comprehensively explored the role of EGCG in high glucose-induced myocardial cell injury and aging.
摘要：
The intracellular polysaccharides of Aspergilluscristatus (IPSs) from Fuzhuan brick tea have beendemonstrated to improve immune function linked to modulating the gutmicrobiota. Herein, to further investigate the efficacy of IPSs tomaintain gut homeostasis, the protection of the purified fractionof IPSs (IPSs-2) on the mice with colitis induced by dextran sulfatesodium (DSS) and the underlying mechanisms were explored in this study.The results revealed that IPSs-2 alleviated the typical symptoms ofcolitis and suppressed the excessive inflammatory mediators, regulatingthe genes related to inflammatory responses in the colon at the mRNAlevel. Meanwhile, IPSs-2 treatment reinforced the intestinal barrierfunction by ameliorating the DSS-induced histological injury, facilitatingthe differentiation of goblet cells to enhance Mucin-2 generation,and enhancing the expression of tight junction proteins to alleviatecolitis. In addition, IPSs protected against colitis by promotingthe production of short-chain fatty acids (SCFAs), the activationof SCFAs receptors, and the leverage of the gut microbiota via the enrichment of Bacteroides, Parabacteroides, Faecalibacterium, Flavonifractor_plautii, and Butyricicoccus, linking with reducing inflammation and repairing intestinal barrierfunction. Overall, our research revealed the therapeutic potentialof IPSs-2 as a prebiotic for attenuating inflammatory bowel diseaseand provided a rationale for future investigation.
通讯机构：
[Yanlin Li; Xingyao Xiong] A;Authors to whom correspondence should be addressed.<&wdkj&>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha 410128, China<&wdkj&>Hunan Mid-Subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center, Changsha 410128, China<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Kunpeng Institute of Modern Agriculture, Foshan 528200, China<&wdkj&>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China
摘要：
This study employed a combination of ultraviolet spectrophotometry, LC-ESI-MS/MS system, and RNA-sequencing technology; the extracts and isolation of total RNA from the red and yellow leaf strains of red maple (Acer rubrum L.) at different developmental stages were subjected to an intercomparison of the dynamic content of chlorophyll and total anthocyanin, flavonoid metabolite fingerprinting, and gene expression. The metabonomic results indicated that one hundred and ninety-two flavonoids were identified, which could be classified into eight categories in the red maple leaves. Among them, 39% and 19% were flavones and flavonols, respectively. The metabolomic analysis identified 23, 32, 24, 24, 38, and 41 DAMs in the AR1018r vs. AR1031r comparison, the AR1018r vs. AR1119r comparison, the AR1031r vs. AR1119r comparison, the AR1018y vs. AR1031y comparison, the AR1018y vs. AR1119y comparison, and the AR1031y vs. AR1119y comparison, respectively. In total, 6003 and 8888 DEGs were identified in AR1018r vs. AR1031r comparison and in the AR1018y vs. AR1031y comparison, respectively. The GO and KEGG analyses showed that the DEGs were mainly involved in plant hormone signal transduction, flavonoid biosynthesis, and other metabolite metabolic processes. The comprehensive analysis revealed that caffeoyl-CoA 3-O-methyltransferase (Cluster-28704.45358 and Cluster-28704.50421) was up-regulated in the red strain but down-regulated in the yellow strain, while Peonidin 3-O-glucoside chloride and Pelargonidin 3-O-beta-D-glucoside were up-regulated in both the red and yellow strains. By successfully integrating the analyses on the behavior of pigment accumulation, dynamics of flavonoids, and differentially expressed genes with omics tools, the regulation mechanisms underlying leaf coloring in red maple at the transcriptomic and metabolomic levels were demonstrated, and the results provide valuable information for further research on gene function in red maple.
摘要：
The protein levels in a diet are correlated with immunity but the long-term intake of excessive protein can compromise various aspects of health. L-theanine regulates immunity and protein metabolism; however, how its regulatory immunity effects under a high-protein diet are unclear. We used proteomics, metabonomics, and western blotting to analyze the effects of diets with different protein levels on immune function in rats to determine the role of L-theanine in immunity under a high-protein diet. The long-term intake of high-protein diets (>= 40% protein) promoted oxidative imbalance and inflammation. These were alleviated by L-theanine. High-protein diets inhibited peroxisome proliferator-activated receptor (PPAR)alpha expression through the inter-leukin (IL)-6/signal transducer and activator of transcription (STAT)3 pathway and mediated inflammation. L-theanine downregulated anti-fatty acid-binding protein 5 (FABP5), inhibited the IL-6/STAT3 axis, and reduced high-protein diet-induced PPAR alpha inhibition. Therefore, L-theanine alleviates the adverse effects of high-protein diets via the FABP5/IL-6/STAT3/PPAR alpha pathway and regulates the immunity of normally fed rats through the epoxide hydrolase (EPHX)2/nuclear factor-kappa B inhibitor (I kappa B)alpha/triggering receptor expressed on myeloid cells (TREM)1 axis.
通讯机构：
[Ai-qing Zhao] S;[Zhong-hua Liu; Ai-ling Liu] N;National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi, China<&wdkj&>National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan, China
摘要：
Polyphenol oxidase (PPO) is a metalloenzyme with a type III copper core that is abundant in nature. As one of the most essential enzymes in the tea plant (Camellia sinensis), the further regulation of PPO is critical for enhancing defensive responses, cultivating high-quality germplasm resources of tea plants, and producing tea products that are both functional and sensory qualities. Due to their physiological and pharmacological values, the constituents from the oxidative polymerization of PPO in tea manufacturing may serve as functional foods to prevent and treat chronic non-communicable diseases. However, current knowledge of the utilization of PPO in the tea industry is only available from scattered sources, and a more comprehensive study is required to reveal the relationship between PPO and tea obviously. A more comprehensive review of the role of PPO in tea was reported for the first time, as its classification, catalytic mechanism, and utilization in modulating tea flavors, compositions, and nutrition, along with the relationships between PPO-mediated enzymatic reactions and the formation of functional constituents in tea, and the techniques for the modification and application of PPO based on modern enzymology and synthetic biology are summarized and suggested in this article.
关键词：
White tea;Rucheng Baimao;Drying treatment;Aroma
摘要：
Rucheng Baimao (Camellia pubescens) is an excellent raw material for manufacturing white tea. Drying is the last step in white tea processing and is essential for white tea's aroma. In this study, aroma sensory evaluation combined with volatile compound analysis reveals the characteristic aroma differences of Rucheng Baimao white tea with oven drying (OD), air drying (AD), and sun drying (SD) treatments. Sensory results showed that OD samples had a stronger floral and grassy aroma, AD samples exhibited a more pronounced fresh and pekoe aroma, and SD samples exhibited a unique sunshine odor. Thirty compounds were screened as differential aroma-active compounds of the aroma difference of Rucheng Baimao white tea with three drying treatments using multivariate statistical analysis and relative odor activity value (ROAV >= 1) analysis. The weighted correlation network analysis of the differential compounds revealed the potential association between volatiles and aroma. The results showed that 17 compounds (such as hexanal and p-cymene) were significantly positively correlated with the grassy aroma and 21 compounds (such as linalool and benzeneacetaldehyde) were significantly posi-tively correlated with the floral aroma. Most of these compounds were abundant in OD samples, contributed to its aroma characteristics.
通讯机构：
[Kunbo Wang; Juan Li] A;Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients & Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China
摘要：
Epigallocatechin gallate (EGCG) is an important contributor to bitterness and astringency in summer tea leaves; however, the transcriptional regulatory mechanisms of EGCG biosynthesis remain unclear. In this study, EGCG content was significantly decreased after foliar spraying with nano-Se fertilizers in tea leaves. A WRKY transcription factor (TF), CsWRKY70, was found to be positively related to EGCG content. The open reading frame of CsWRKY70 was 891 bp encoding 296 amino acids. CsWRKY70 is localized to the nucleus and has transcriptional activation activity. The electrophoretic mobility shift assay indicated that CsWRKY70 can directly bind to the promoters of CsLAR and CsUGT84A containing W-box (5 '-C/TTGACT/C-3 ') sequences. Dual-luciferase reporter experiment verified that CsWRKY70 activated CsLAR and CsUGT84A expressions in tobacco leaves. In summary, these results demonstrated that CsWRKY70 may reduce EGCG biosynthesis by inhibiting the CsLAR and CsUGT84A expressions under nano-Se treatment. Our findings provide new insight into the regulatory mechanism of WRKY TFs involved in catechin biosynthesis and offer a theoretical basis for breeding low or high EGCG content tea cultivars.
通讯机构：
[Shanhan Cheng; Jialei Ji] A;Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
摘要：
Cabbage (Brassica oleracea var. capitata) is a vegetable rich in glucosinolates (GSLs) that have proven health benefits. To gain insights into the synthesis of GSLs in cabbage, we systematically analyzed GSLs biosynthetic genes (GBGs) in the entire cabbage genome. In total, 193 cabbage GBGs were identified, which were homologous to 106 GBGs in Arabidopsis thaliana. Most GBGs in cabbage have undergone negative selection. Many homologous GBGs in cabbage and Chinese cabbage differed in expression patterns indicating the unique functions of these homologous GBGs. Spraying five exogenous hormones significantly altered expression levels of GBGs in cabbage. For example, MeJA significantly upregulated side chain extension genes BoIPMILSU1-1 and BoBCAT-3-1, and the expression of core structure construction genes BoCYP83A1 and BoST5C-1, while ETH significantly repressed the expression of side chain extension genes such as BoIPMILSU1-1, BoCYP79B2-1, and BoMAMI-1, and some transcription factors, namely BoMYB28-1, BoMYB34-1, BoMYB76-1, BoCYP79B2-1, and BoMAMI-1. Phylogenetically, the CYP83 family and CYP79B and CYP79F subfamilies may only be involved in GSL synthesis in cruciferous plants. Our unprecedented identification and analysis of GBGs in cabbage at the genome-wide level lays a foundation for the regulation of GSLs synthesis through gene editing and overexpression.
摘要：
The most important physiological processes in insects are those related to reproduction and development. Ecdysone is an essential hormone in insects that controls various physiological processes, including reproduction and development. E74A, a subtype of the essential ecdysone-induced transcription factor E74, affects the reproductive systems of many insects. Uncertainty exists regarding the molecular mechanism of E74A in non-model insect reproduction processes. Using bioinformatics analysis, we determined that Chilo suppressalis E74A shared the highest homology with E74 in Ostrinia furnacalis which belongs to the ETS superfamily. By characterizing the spatiotemporal expression profile of CsE74A from different developmental stages and tissues, we found that CsE74A expression levels were highest in female pupae on the 4th day and in the head of female pupa. Knockdown of CsE74A resulted in delayed oocyte maturation and reduced yolk deposition. Additionally, the expression level of vitellogenin (Vg), beta FTZ-F1, and E93, which are associated with vitellogenesis and the ecdysone pathway, were also downregulated in the E74A silencing group. Collectively, our findings demonstrate that CsE74A not only plays a critical role in the reproductive processes of C. suppressalis but may also participate in the transcriptional regulation of genes involved in the ecdysone pathway.
通讯机构：
[Sheng Zhang] N;National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, Hunan, China
关键词：
Obesity;Functional components of tea;Fat metabolism;Glucose metabolism;Liver function;Gut microbiome;Clinical research
摘要：
Obesity caused by poor eating habits has become a great challenge faced by public health organizations worldwide. Optimizing dietary intake and ingesting special foods containing biologically active substances (such as polyphenols, alkaloids, and terpenes) is a safe and effective dietary intervention to prevent the occurrence and development of obesity. Tea contains several active dietary factors, and daily tea consumption has been shown to have various health benefits, especially in regulating human metabolic diseases. Here, we reviewed recent advances in research on tea and its functional components in improving obesity-related metabolic dysfunction, and gut microbiota homeostasis and related clinical research. Furthermore, the potential mechanisms by which the functional components of tea could promote lipid-lowering and weight-loss effects by regulating fat synthesis/metabolism, glucose metabolism, gut microbial homeostasis, and liver function were summarized. The research results showing a "positive effect" or "no effect" objectively evaluates the lipid-lowering and weight-loss effects of the functional components of tea. This review provides a new scientific basis for further research on the functional ingredients of tea for lipid lowering and weight loss and the development of lipid-lowering and weight-loss functional foods and beverages derived from tea.(c) 2022 Elsevier Inc. All rights reserved.
摘要：
The leaf is an important plant organ and is closely related to agricultural yield. Photosynthesis plays a critical role in promoting plant growth and development. Understanding the mechanism of leaf photosynthesis regulation will help improve crop yield. In this study, the pepper yellowing mutant was used as the experimental material, and the photosynthetic changes of pepper leaves (yl1 and 6421) under different light intensities were analyzed by chlorophyll fluorimeter and photosynthesis meter. Changes in proteins and enrichment of phosphopeptides in pepper leaves were determined. The results showed that different light intensities had significant effects on the chlorophyll fluorescence and photosynthetic parameters of pepper leaves. The differentially expressed proteins (DEPs) and differentially expressed phosphorylated proteins (DEPPs) were mainly involved in photosynthesis, photosynthesis-antenna proteins, and carbon fixation in photosynthetic organisms. In yl1 leaves, the phosphorylation levels of photosynthesis and photosynthesis-antenna proteins LHCA2, LHCA3, PsbC, PsbO, and PsbP were lower under low light treatment, but significantly higher under high light intensity compared with wild-type leaves. In addition, many proteins involved in the carbon assimilation pathway, including TKT, Rubisco, and PGK, were phosphorylated, and this modification level was significantly higher in yl1 than in the wild type under high light intensity. These results provide a new perspective for studying the photosynthesis mechanism of pepper under different light intensities.
作者：
Chun Guo;Ying He;Xiyao Zeng;Xingyao Xiong;Ping Qiu;...
期刊：
中草药：英文版,2023年15(2):278-283 ISSN：1674-6384
通讯作者：
Huang, X.
作者机构：
[Chun Guo] Center for Medical Research and Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, China;[Ping Qiu] Hunan Zhengqing Pharmaceutical Group Co., Ltd., Huaihua 418000, China;[Xingyao Xiong] College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410125, China;[Xueshuang Huang] Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, Hunan University of Medicine, Huaihua 418000, China
通讯机构：
[Huang, X.] H;Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, China
关键词：
chloroplast DNA;genetic diversity;phylogeny;population variation;Sinomenium acutum (Thunb.) Rehd. et Wils
摘要：
Introduction: Lonicera japonica flos, a well-known herbal medicine in Asian countries, has been widely used for the treatment of type 2 diabetes (T2D). The gut microbiota and relevant metabolites have been shown to be involved in the development of T2D. This study was designed to investigate the effects of Lonicera japonica flos on fecal microbiota from humans with T2D using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) system. Methods: An aqueous extract of Lonicera japonica flos (LJE) was prepared. Colorimetry and high-performance liquid chromatography (HPLC) were used to analyze the chemical composition of LJE. Three colonic compartments of SHIME were inoculated with feces from T2D donors, and LJE was added to SHIME to simulate daily oral administration for 7 days. The microbial community and the concentrations of short-chain fatty acids (SCFAs) of colonic samples were analyzed by 16 S rRNA sequencing technology and gas chromatography, respectively. In addition, polyphenols in LJE before and after fermentation by fecal microbiota were measured using HPLC. Results: LJE is rich in polyphenols and polysaccharides and alters the microbial community, as indicated by the decreased relative abundance of the detrimental genera Shigella and Lactobacillus and the increased abundance of SCFA-producing bacteria, including the genera Prevotella and Bacteroides. LJE significantly promoted SCFA production, including acetic, propionic, and butyric acids, in each colonic compartment. Furthermore, chlorogenic acid in LJE was biotransformed by fecal microbiota. Conclusions: LJE exerted beneficial regulation on the T2D fecal microbial community. The regulatory effects of gut microbiota may partly contribute to the therapeutic action of Lonicera japonica flos on T2D.
摘要：
Scenting tea with Jasminum sambac is beneficial to forming a unique taste of jasmine tea, which is regulated by numerous compounds. To investigate the relationship between metabolites in jasmine and jasmine tea, as well as the impact of metabolites on the characteristic taste of jasmine tea, the liquid chromatography-mass spectrometry, sensory evaluation, and multivariate analysis were applied in this study. A total of 585 and 589 compounds were identified in jasmine tea and jasmine, respectively. After scented, jasmine tea added 70 compounds, which were believed to come from jasmine flowers. Furthermore, seventy-four compounds were identified as key characteristic compounds of jasmine tea, and twenty-two key differential metabolite compounds were believed to be used to distinguish jasmine tea scented differently and contribute to the taste of jasmine tea. Additionally, the relationship between taste compounds and aroma quality was also explored, and it was found that five compounds were positively correlated with the aroma properties of jasmine tea and seven compounds were negatively correlated with the aroma properties of jasmine tea. Overall, these findings provided insights into the future study of the mechanism of taste formation in jasmine tea and provided the theoretical basis for the production of jasmine tea.
