摘要：
Uncaria rhynchophylla is an evergreen vine plant, belonging to the Rubiaceae family, that is rich in terpenoid indole alkaloids (TIAs) that have therapeutic effects on hypertension and Alzheimer's disease. GATA transcription factors (TF) are a class of transcription regulators that participate in the light response regulation, chlorophyll synthesis, and metabolism, with the capability to bind to GATA cis-acting elements in the promoter region of target genes. Currently the charactertics of GATA TFs in U. rhynchophylla and how different light qualities affect the expression of GATA and key enzyme genes, thereby affecting the changes in U. rhynchophylla alkaloids have not been investigated. In this study, 25 UrGATA genes belonging to four subgroups were identified based on genome-wide analysis. Intraspecific collinearity analysis revealed that only segmental duplications were identified among the UrGATA gene family. Collinearity analysis of GATA genes between U. rhynchophylla and four representative plant species, Arabidopsis thaliana, Oryza sativa, Coffea Canephora, and Catharanthus roseus was also performed. U. rhynchophylla seedlings grown in either red lights or under reduced light intensity had altered TIAs content after 21 days. Gene expression analysis reveal a complex pattern of expression from the 25 UrGATA genes as well as a number of key TIA enzyme genes. UrGATA7 and UrGATA8 were found to have similar expression profiles to key enzyme TIA genes in response to altered light treatments, implying that they may be involved in the regulation TIA content. In this research, we comprehensively analyzed the UrGATA TFs, and offered insight into the involvement of UrGATA TFs from U. rhynchophylla in TIAs biosynthesis.
摘要：
Understanding the influencing effect of meteorological factors and air pollutants in the campus plot and the relationship between them is an important topic in the planning and design of campus green space. The changes of pollutant concentrations and meteorological factors in campus green space have certain patterns and specific influencing factors. In this study, we selected four sample plots in Nanjing Forestry University as the research objects, and collected various environmental parameters of the four plots on July 25, 2022. The results showed that the main influences of meteorological factors are the type of the underlying surface of the site, the degree of plant canopy density and the shade coverage area of the building. These factors mainly have a great influence on the value of temperature and humidity. The comprehensive influencing factors can be concluded that the cooling and humidifying effect of the site is ranked as follows: forest > lawn > asphalt road > concrete Square. The main influencing factors of pollutants are: illumination, wind speed, temperature and relative humidity. Among them, illumination and temperature have a negative correlation with PM(2.5), wind speed and relative humidity have a positive correlation with PM(2.5). Our research shows that the adjustment of campus green space factors can reduce the concentration of pollutants by changing the meteorological factors.
摘要：
Caffeine, a primary flavor component in tea, has been the subject of intense research. With the goal of shedding light on the complex regulatory processes governing caffeine biosynthesis in tea plants, liquid chromatography coupled with mass spectrometry (LC-MS), transcriptomics, and small RNA analyses were employed on diverse tea cultivars such as 'Jianghua Kucha' [including 'Xianghong 3' (XH3H) and 'Kucha 3' (KC3H)], 'Fuding Dabaicha' (FDDB), 'Yaoshan Xiulv' (YSXL), and 'Bixiangzao' (BXZ). The results showed that the caffeine level in 'Jianghua Kucha' was significantly higher than that in other tea plant cultivars. In addition, weighted gene co-expression network analysis indicated that that the CsbHLH1 gene might play a pivotal role as a potential hub gene related to the regulation of caffeine biosynthesis. Subcellular localization analysis showed that the CsbHLH1 protein was localized in the nucleus of the cells. Moreover, CsbHLH1 suppresses the transcription of TCS1 by binding to the TCS1 promoter, as evidenced by a yeast one-hybrid assay, an electrophoretic mobility shift assay, and dual luciferase analysis. In addition, a microRNA, miR1446a, was identified that directly cleaves CsbHLH1, leading to an increase in caffeine levels. Therefore, our findings imply that CsbHLH1 binds to the TCS1 promoter (-971 to -1019 bp) to reduce its expression, thereby negatively regulating caffeine biosynthesis. On the other hand, miR1446a enhances the biosynthesis of caffeine by suppressing the expression of CsbHLH1. This work enhances our understanding of the molecular mechanisms of caffeine biosynthesis in tea plants and offers potential directions for manipulating caffeine levels in future tea cultivation.
摘要：
Nelumbo nucifera, or sacred lotus, has been valuable for us to use as vegetable, functional food, and herb medicine for over 2,000 years. The purpose of this article is to systematically review the traditional/modern uses, chemical compositions and pharmacological activities on different parts of N. nucifera. Traditionally, this plant has been used to treat chronic dyspepsia, hematuria, insomnia, nervous disorders, cardiovascular diseases, and hyperlipidemia. Now, phytochemical investigations on different parts of N. nucifera have indicated a wide spectrum of at least 255 constituents belonging to different chemical groups, including proteins, amino acids, polysaccharides, starch, flavonoids, alkaloids, essential oils, triterpenoids, steroids, and glycosides. Meanwhile, its pharmacological activities, including anti-obesity, antioxidant, anti-inflammatory, cardiovascular, hepatoprotective, hypoglycemic, hypolipidemic, antitumor, memory-improving and antiviral activities, have also been reviewed, together with its applications in health food industry and clinic uses of its single plant or herbal formulae. Herein, this review will provide state-of-the-art overview on its traditional and modern uses in food industry and medicines, together with the comprehensive profiles of phytochemicals, and health promoting bioactivities of this valuable plant. In addition, the new perspectives and future challenges in the research on lotus are also outlined.
关键词：
gut microbiota;mechanism;metabolic diseases;tea polysaccharide
摘要：
Tea polysaccharides (TPS) are regarded as some of the main bioactive constituents of tea made from the leaves and buds of the tea plant (Camellia sinensis L.). An increasing number of studies have demonstrated that TPS can reduce the risk of type 2 diabetes, obesity, and other metabolic diseases. However, the potential mechanisms responsible for antidiabetic and antiobesogenic activities of TPS remain unclear. Therefore, the cellular and physiological mechanisms that underlie the antidiabetic and antiobesogenic effects, including antioxidant and anti-inflammation effects, inhibition of digestive enzymes, prevention of macronutrient absorption, and expression of gene and protein, were summarized in this review. Furthermore, the gastrointestinal functions of TPS and the role of gut microbiota in the prevention and treatment of metabolic diseases were discussed. It is expected that the present review will be helpful for enhancing our knowledge about the health-promoting effects of TPS on metabolic diseases and stimulating further works on TPS.
摘要：
For better exploring of ecological characteristic of traditional plant landscape, after investigation and analysis of plants species and plants space, this paper found out there are 54 cultivars of main plants in Yuelu Academy. Nowadays, they show stronger ecological adaptability. The academy's plants have significant seasonal landscape aspect and profound cultural meaning. They function in constructing the unique academy space and can meet modern city's leisure need. It should be adopted and protected for lasting landscape with national characteristics.
摘要：
The water environmental capacity is not only the basic foundation in objective management of water environment, but also the constraint condition for the planning of water environment and its surrounding environment. Dongjiang Lake is the location of strategic water resources reserves in Xiangjiang River Basin urban agglomeration, and the Dongjiang Lake Scenic Area is a national scenic area that combines the ecological and tourism functions. Based on the statistics of water environment pollution in Dongjiang Lake Scenic Area, this paper conducts quantitative research and analysis of its water environmental capacity, hoping to conduct phased control of target volume of pollution load into Dongjiang Lake Scenic Area and provide data support to various follow-up special plans in the scenic area.
会议名称：
29th International Horticultural Congress on Horticulture - Sustaining Lives, Livelihoods and Landscapes (IHC) / International Symposia on Water, Eco-Efficiency and Transformation of Organic Waste in Horticultural Production
摘要：
Citrus is one of the most popular fruit crops in south part of China. Water deficit is a serious environmental stress that affects citrus growth, fruit production and quality. The objective of this study was to quantify the effects of water deficit stress on proline, soluble sugars content and jasmine acid (JA) biosynthesis and correlative gene expression in endogenous JA biosynthesis in 'Yamasitaka' (Citrus unshiu Marc) and 'Newhall' navel orange (Citrus sinensis (L.) Osbeck). Results show that accumulation of JA, content of proline and soluble sugar were significantly increased by water stress, however, content of chlorophyll decreased with increasing water stress. Real-time quantitative PCR, used to analyze correlative gene expression in endogenous JA biosynthesis, showed that CitAOS expression gradually increased with water stress. There was a significant positive relationship between content of JA and CitAOS, which indicated that CitAOS may play a more important role than the genes involved in JA biosynthesis in citrus.
摘要：
Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents an efficient strategy to confer genetic resistance against a broad range of phytopathogenic fungi. In lemon (Citrus limon (L.) Burm. f.), fungal diseases cause economic losses in the field as well as in postharvest management. In this study, fruits collected from two different transgenic lemon clones (E23 and E24) expressing a chitinase gene (chit42) from Trichoderma harzianum were tested to investigate the level of resistance against Penicillium digitatum, P. italicum, Colletotrichum gloesporioides and Botrytis cinerea. Fruits were inoculated with conidial suspensions of these fungi, incubated in a climatic chamber and symptom development was evaluated at 6, 14 and 21 days after inoculation. While fruits from either transgenic clone inoculated with C. gloesporioides and B. cinerea did not show mould symptoms during the whole evaluation period, similar fruits inoculated with P. digitatum or P. italicum showed reduced lesion area (50%) in comparison with the non-transgenic control fruits after 6 days and no difference after 14 days. The transgenic lemon clones, obtained from a highly appreciated lemon cultivar, did not show any difference in morphological and qualitative fruit traits compared to the non-transgenic control. These results suggest that their use could represent an alternative for sustainable management of lemon postharvest diseases.
摘要：
The genetic structure of the Citrus tristeza virus (CTV) population in the Hunan Province, China, was studied by capillary electrophoresis-single strand conformation polymorphism (CE-SSCP), multiple molecular markers (MMM), and bioindexing. A total of 17 samples collected in Yizhang and Yong Xing counties were tested by tissue print-ELISA, and 10 were found CTV infected. CE-SSCP analysis showed different profiles some of them indicating composite patterns. MMM analysis revealed the presence of the VT and T3 genotypes, either individually or in combination. One more isolate from Chenzou (HU-PSTS) showing the most complex CE-SSCP profile and a T36 + VT + T30 genotype was further investigated by graft inoculation on indicator plants and phylogenetic analysis of the p25 gene. Bioindexing revealed a very severe seedling yellows reaction and an unusual stem pitting on sour orange. Three p25 clones obtained from this isolate, showed different CE-SSCP profiles and were phylogenetically close to recombinant Hawaiian and Indian strains.
关键词：
pomegranate water stress;growth;nitrogen metabolism
摘要：
Water deficit is one of the serious environmental stresses that affect pomegranate growth, fruit production and quality. Pomegranate is one of the most valuable fruit tree crops in China. The objective of this study was to quantify the effects of water-deficit stress on leaf and shoot growth and leaf concentrations of arginine, praline, polyamines, and related metabolites in pomegranate compared with non-water-deficit treated pomegranate seedlings. Replicate pots, each containing one young seedling in 2 L of soil, were subjected to water deficit by withholding water or were maintained under well-watered (control) conditions. Water stress reduced both total and shoot dry matter (DW), but increased root DW and the value of root/shoot, compared to the control. Plant height and stem diameter were reduced by 26.53 and 34.93% respectively. Water stress decreased protein concentration in leaves of pomegranate, under well watered condition, leaves protein content of pomegranate was 16.72 mg/g fresh weight. However, when soil water was withheld to 20% water content treatment, leaves protein content was 7.19 mg/g fresh weight, decreased by 52.69%. Water stress increased leaf concentration of NH3-NH4 +, arginine and proline. Under well watered condition, NH3-NH4 +, Arginine and Proline concentrations of young, fully expanded leaves of pomegranate were the lowest, and these values were the highest at 20% water content treatment. Compared with control plants, concentrations of NH3-NH4 + in pomegranate leaves at 20% water content treatment increased 149.93%; free arginine concentration increased 79.96%; and concentration of proline increased 171.62%.
摘要：
To establish a green fluorescent protein(GFP)-based cellular model for screening GLP-1 receptor ligands from Traditional Chinese Medicinal herbs. A eukaryotic expression vector pC MV6-GLP-1R-GFP was c
摘要：
Seasonal drought is the main environmental stress that affected yield and quality of Citrus fruits in China. The effects of water deficit stress on abscisic acid (ABA), jasmonic acid (JA) and physiological characteristic were examined for 2 years old potted Newhall Orange [C. sinensis (L) Osbeck] and Yamasitaka [C. unshin Marc] mandarin seedlings on Poncirus trifoliata (L) rootstocks. The result showed: ABA and JA content was significantly affected by water stress in Yamasitaka and Newhall orange. Compared with CK, the content of ABA under 7% soil water treatment was over 2-fold. The content of ABA in Yamasitaka showed no significant different among CK, 21% and 14% water treatments, however, the ABA content upgrade to 10.42 ng/g under 7% water treatment. The content of JA under 7% soil water treatment in Newhall orange was approximate 300% more than that of CK. There was a similar trend of JA content in Yamasitaka, the content of JA under well water condition was only 63.2% of that under 7% soil water treatment. Regardless of treatment, the content of JA in Yamasitaka is much less than that in Newhall orange. Water stress could induce proline and reductive sugar accumulation in both two cultivars, the content of proline and reductive sugar reach its maximum value under 7% soil water treatment.
摘要：
Cultural landscape as extremely valuable historical and cultural resources are not renewable, minority folk, folk culture resources in Wuling Mountain Area as the important content of the development, in the new rural planning and construction process, we should pay attention to the value of traditional folk culture resources, insist on the principle of combining the protection and utilization. Make construction and development of the folk culture of the ethical region and multicultural harmonious coexistence. This paper taking Zhangjiajie Tujia characteristics of village planning as a case, investigate its humanitarian landscape resource and conservation value, according to the principle of the protection of the first and the development of the second under the premise of the protection and restoration of the original buildings and folk culture resources, using eco-planning and design innovative mode. With the rapid development of economic construction, China's urbanization speed. According to the 2010 census data, China's urban population surge by about 30% in 2000 to about 50% in 2010. According to "China's rural poverty alleviation and development program (2011-2020), and the wuling mountain area regional development and poverty alleviation works planning (2011-2020), The state will increase the strength of the western development, China will further accelerate the new rural construction. In the construction of new countryside, however, some planning designers in city planning theory will be big demolition to build rural, One-sided pursuit of formal beauty or some kind of design technique in the construction of new rural waste of resources and damage to the ecosystem. Humanities landscape, is artificially created, merge with the natural landscape around, there's a certain cultural characteristics and various cultural phenomenon, the fluctuation in five thousand of the Chinese nation in the social practice and profound created the material wealth and spiritual wealth, and many become the human society civilization achievements with distinctive features. Urbanization process had different degrees of increase of people's living standard, but the national characteristic culture, urbanization, the ancestral culture of valuable material and spiritual wealth or development of the folk culture of ethnic minority areas and cultural diversity coexist status remain, and embodies human harmony with the natural environment, social economic and cultural sustainable development is the main research contents in this paper. In ethnic minority areas zhangjiajie yongding district Shi Yan ping village as example, the study of conservation and utilization of people embraced, wuling mountain area for regional development and poverty alleviation attack planning implementation process to provide theoretical reference.
通讯机构：
[He, Li Bo] H;Hunan Agr Univ, Coll Hort & Landscape Architecture, Changsha 410128, Hunan, Peoples R China.
会议名称：
International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2012)
会议时间：
AUG 10-12, 2012
会议地点：
Zhangjiajie, PEOPLES R CHINA
会议主办单位：
[He, Li Bo;Xiong, Xing Yao] Hunan Agr Univ, Coll Hort & Landscape Architecture, Changsha 410128, Hunan, Peoples R China.
会议论文集名称：
Applied Mechanics and Materials
关键词：
Eight scenes of yuelu academy;Landscape restoration;Yuelu academy
摘要：
Yuelu Academy is one of the four most prestigious academies in the history, its architecture part had been reconstructed in 1980's, but the garden landscape lacked unified design. Nowadays, the garden landscape of academy is losing its poetic imagery gradually. Under the principle of respecting history and spreading garden tradition, the conception of improving landscape axis for the academy and restoring Eight Scenes of Yuelu Academy is proposed for the overall restoration of the academy landscape. It is meaningful for setting a good example for the Chinese classical academy's garden and replenishing the traditional garden art.
会议名称：
28th Int Horticultural Congress on Science and Horticulture for People (IHC) / Int Symposium on Genomics and Genetic Transformation of Horticultural Crops
摘要：
Antibiotic or herbicide resistant selective genes are widely used as markers in transformation experimentation for the selection of transgenic regenerants from wild type tissues. However, the potential hazards on ecological environment and human health of such marker genes remaining in the transgenic plants have caused common social worries and concerns. In the present study, a chemical-inducible auto-excision marker free vector system with Cre/loxP site-specific recombination system and an inducible expression system XVE was used in citrus. The result indicated that marker gene excision in this vector could occur in citrus transformants. When regenerated buds reached to 2-4 mm with 1-2 mm explants, they were cut and transformed to chemical-inducible medium (MS+ 1mg/L 6-BA + 4 mu M beta-estradiol). After reaching 1 cm, shoots were cut and subcultured on chemical-inducible medium for another 30 days. Molecular detection and fluorescence observation indicated that different gene recombination and partially excisions existed in various transgenic citrus clones. The complete excision of marker genes was detected in 2 of the 80 transgenic clones. The suitable conditions for the induction of removing marker genes were discussed.
会议名称：
28th Int Horticultural Congress on Science and Horticulture for People (IHC) / Int Symposium on Citrus, Bananas and Other Tropical Fruits Under Subtropical Conditions
会议时间：
AUG 22-27, 2010
会议地点：
Lisbon, PORTUGAL
会议主办单位：
[Xie, Shenxi;Liu, Qiang;Xiong, Xingyao] Hunan Agr Univ, Coll Hort, Changsha 410128, Hunan, Peoples R China.^[Xie, Shenxi] Nat Ctr Citrus Improvement, Changsha Subcenter, Changsha 410128, Peoples R China.^[Lovatt, C. J.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA.
摘要：
In south China, rainfall is unequally distributed in different seasons; therefore, water deficit is one of the serious environmental stresses that affect citrus growth, fruit production and quality. It is important for citrus production to understand the relationship between water stress and photosynthetic characteristics. In this research, effect of water stress on citrus photosynthesis characteristics was systematically studied using Yamasitaka, Newhall orange and Sha-tian pomelo as materials Chlorophyll content of citrus was significantly decreased by water stress. There was a positive relationship between relative water content of soil and the volume of chlorophyll a and chlorophyll b. Compared with chlorophyll b, chlorophyll a was more easily damaged by water stress. Under the greatest drought stress treatment, 20% of field capacity (FC), the net photosynthetic rates (Pn) of Sha-tian pomelo, Newhall orange, Yamasitaka were 3.44, 1.21, and 1.47 mu mol m(-2) s(-1), respectively, which were decreased over 90% compared with the well-watered control. There were positive relationships between relative water content of soil, Pn, Transpiration rate (Tr), stomatal conductance (Gs). Under well water conditions, the diurnal variation of Pn of Sha-tian pomelo, Yamasitaka and Newhall orange had bimodal curve, but the value and apparent time of peak rates differed among three cultivars. There was an obvious "noon break" or midday depression phenomenon. Under water stress, the diurnal Pn of Sha-tian pomelo had one peak while Yamasitaka, Newhall orange had present two peaks. The diurnal Pn of Yamasitaka was bimodal curve, under well water, 60 and 40% FC treatments. However under 20% FC treatment, Yamasitaka had a single curve.
