Effects of solid-state fermentation with Bacillus subtilis LK-1 on the volatile profile, catechins composition and antioxidant activity of dark teas
作者：
Xiao, Leike;Yang, Chenghongwang;Zhang, Xilu;Wang, Yuanliang;Li, Zongjun;...
期刊：
Food Chemistry: X ,2023年19:100811 ISSN：2590-1575
通讯作者：
Xiao, Y;Zhu, Mingzhi;Chen, YL
作者机构：
[Li, Zongjun; Xiao, Yu; Zhang, Xilu; Xiao, Y; Yang, Chenghongwang; Wang, Yuanliang; Xiao, Leike] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[Xiao, Yu; Liu, Zhonghua; Xiao, Y; Zhu, Mingzhi] Hunan Agr Univ, Coll Hort, Key Lab, Minist Educ Tea Sci, Changsha 410128, Peoples R China.;[Chen, Yulian] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.;[Liu, Zhonghua; Zhu, Mingzhi] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct Ing, Changsha 410128, Peoples R China.
通讯机构：
[Chen, YL ; Xiao, Y ; Zhu, MZ] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Hort, Key Lab, Minist Educ Tea Sci, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.
关键词：
Bacillus subtilis;Dark tea;Volatile organic compounds;Solid-state fermentation;Catechins composition;Multivariate statistical analysis
摘要：
In this study, the solid-state fermentation (SSF) of dark tea was carried out using Bacillus subtilis LK-1, which was isolated from Fu brick tea (FBT). The effects of SSF with B. subtilis on volatile organic compounds (VOCs), non-volatile metabolites, and antioxidant activities of dark tea was investigated. A total of 45 VOCs were identified, primarily consisting of ketones (18), hydrocarbons (8), aldehydes (7), and alcohols (6). Following fermentation, the content of key odor active substances such as linalool, β-ionone, and 3,5-octadiene-2-one significantly increased, resulting in an enhanced floral and fruity aroma of dark tea. Furthermore, new flavor substances like geranyl isovalerate and decanal were produced during SSF, enriching the aroma profile of dark tea. Non-ester catechins demonstrated a drastic increase, while ester catechins remarkably decreased after SSF. Furthermore, SSF led to a slight decrease in the total polyphenols content and antioxidant activity of dark tea. There is a close relationship between VOCs and the main non-volatile metabolites during SSF. Overall, this study highlighted the great impact of SSF with B. subtilis on the metabolites of dark tea and provided valuable insights into the role of bacteria in shaping the metabolite profile of FBT. © 2023 The Authors
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英文
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l-Theanine attenuates heat stress-induced proteotoxicity and alterations in carbohydrate and lipid metabolism via heat shock factor 1
作者：
Lin, Ling;Han, Shumin;Gong, Zhihua;Ding, Furong;Liu, Zhonghua* ;...
期刊：
FOOD & FUNCTION ,2023年14(13):6172-6186 ISSN：2042-6496
通讯作者：
Liu, Zhonghua;Xiao, WJ
作者机构：
[Han, Shumin; Gong, Zhihua; Liu, Zhonghua; Xiao, Wenjun; Xiao, WJ; Lin, Ling] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Han, Shumin; Gong, Zhihua; Liu, Zhonghua; Xiao, Wenjun; Xiao, WJ; Lin, Ling] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Han, Shumin; Gong, Zhihua; Liu, Zhonghua; Xiao, Wenjun; Xiao, WJ; Lin, Ling] Hunan Agr Univ, Coinnovat Ctr, Educ Minist Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Ding, Furong] Agr & Rural Bur Shimen Cty, Changde 415300, Hunan, Peoples R China.
通讯机构：
[Liu, ZH; Xiao, WJ ] H;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coinnovat Ctr, Educ Minist Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
摘要：
Extreme heat caused by global warming accelerated the frequency of heat stress (HS). Proteotoxic stress induced by the aggregation of misfolded proteins and metabolic stress triggered by alterations in the metabolism were observed during HS. The activation of heat shock factor 1 (Hsf1) and its interaction with adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) are critical in addressing proteotoxicity and metabolic stress in heat-stressed organisms. Previous studies have shown that l-theanine (LTA) can regulate nutrient metabolism through the AMPK pathway and can alleviate HS. Therefore, we hypothesize that LTA may help in restoring homeostasis by regulating nutrient metabolism under HS. Here, we investigated the effects of LTA on nutrient metabolism in heat-stressed rats and characterized the underlying mechanisms using RNA sequencing and metabonomics. The results showed that LTA alleviated HS-induced liver damage, promoted body weight gain, decreased serum cortisol and enhanced the total protein content. Besides, it regulated the expression of genes related to carbohydrate, lipid and amino acid metabolism and altered metabolite levels. Moreover, LTA inhibited the expression of Hsf1 and heat shock protein 70 (Hsp70), promoted AMPK phosphorylation and the expression of glucose-6-phosphatase catalytic subunit 1 (G6pc), and inhibited the phosphorylation of acetyl-CoA carboxylase 1 (ACC1) in heat-stressed rats. Mechanistically, LTA alleviated HS-induced proteotoxic stress by acting on Hsf1/Hsp70; simultaneously, it promoted AMPK phosphorylation by suppressing Hsf1 expression, which in turn inhibited fatty acid synthesis and hepatic gluconeogenesis, thus alleviating HS-induced metabolic stress. These results suggest that LTA regulates nutrient metabolism through Hsf1/AMPK and alleviates HS-induced proteotoxicity via Hsf1/Hsp70. © 2023 The Royal Society of Chemistry.
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英文
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l-Theanine alleviates heat stress-induced impairment of immune function by regulating the p38 MAPK signalling pathway in mice
作者：
Hu, Yuan;Lin, Ling;Liu, Kehong;Liu, Enshuo;Han, Shumin;...
期刊：
FOOD & FUNCTION ,2023年14(1):335-343 ISSN：2042-6496
通讯作者：
Gong, Zhihua(xiaowenjun88@sina.com);Xiao, Wenjun(xiaowenjun88@sina.com)
作者机构：
[Han, Shumin; Gong, Zhihua; Liu, Kehong; Xiao, Wenjun; Hu, Yuan; Liu, Enshuo; Lin, Ling] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Han, Shumin; Gong, Zhihua; Liu, Kehong; Xiao, Wenjun; Hu, Yuan; Liu, Enshuo; Lin, Ling] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.;[Han, Shumin; Gong, Zhihua; Liu, Kehong; Xiao, Wenjun; Hu, Yuan; Liu, Enshuo; Lin, Ling] Hunan Agr Univ, Coinnovat Ctr Educ Minist Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.;[Han, Shumin; Gong, Zhihua; Liu, Kehong; Xiao, Wenjun; Hu, Yuan; Liu, Enshuo; Lin, Ling] Hunan Agr Univ, Key Lab Evaluat & Utilizat Gene Resources Hort Cr, Minist Agr & Rural Affairs China, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhihua Gong; Wenjun Xiao] K;Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Hunan Agricultural University, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, China
摘要：
With the current trend of global warming, heat stress-induced impairment could seriously endanger human health. l-Theanine is a non-protein amino acid in tea with various biological activities, including immunoregulatory, anti-anxiety, and anti-oxidation. However, its effect on immune function under heat stress and the underlying mechanism are currently unclear. In this study, male BALB/c mice were used as experimental objects to explore the effect of l-theanine on heat stress-induced changes in immune function and its mechanism. Three doses of l-theanine were used: low (100 mg kg−1 d−1), medium (200 mg kg−1 d−1), and high (400 mg kg−1 d−1). Treatment with l-theanine could attenuate the heat stress-induced reductions in body weight and feed intake in mice, alleviate damage in the liver and jejunum, and inhibit the inflammatory factors IL-6, IL-1β, and TNF-α. Aspartate aminotransferase and alanine transaminase activity levels and the malondialdehyde content decreased, while the IgA, IgM, and IgG contents increased in response to l-theanine. It is possible that l-theanine affects the P38 signalling pathway and inhibits the increase in p-P65/P65 caused by the overexpression of HSP27 and regulation of PPAR-γ and Foxp3 proteins, thereby alleviating immune dysfunction caused by heat stress. © 2023 The Royal Society of Chemistry.
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英文
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Effects of long-term administration of theasinensin A on healthy C57BL/6J mice: Enhancing the function of epididymal white adipose tissue and regulating the colonic microenvironment
作者：
Xu, Weiqi;Huang, Yujie;Tao, Sainan;Zhou, Wangting;Peng, Yujia;...
期刊：
Food Chemistry ,2023年403 ISSN：0308-8146
通讯作者：
Zeng, Xiaoxiong(zengxx@njau.edu.cn)
作者机构：
[Chen, Guijie; Huang, Yujie; Xu, Weiqi; Tao, Sainan; Peng, Yujia; Zeng, Xiaoxiong; Kan, Xuhui; Zhou, Wangting; Dong, Wei] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Peoples R China.
通讯机构：
[Zeng, X.] C;College of Food Science and Technology, Jiangsu, China
关键词：
Adipocytic function;Akkermansia muciniphila;Intestinal microbiota;Tea;Theasinensin A
摘要：
The effects of theasinensin A (TSA) on healthy C57BL/6J mice were investigated. Results showed that long-term administration of 100 mg/kg body weight/day TSA might be non-toxic to healthy mice based on the unaltered basal biochemical indicators related to glucose and lipid metabolism, inflammatory factors and hepatic injury. On the contrary, TSA stimulated the rate of lipid turnover and browning of white adipose tissues, accelerated the adipocytic energy mobilization, and then reduced the white adipocytic size, ultimately enhancing resistance to obesity in healthy mice. Furthermore, TSA not only up-regulated the expression of mucin, tight junction protein, and short-chain fatty acids receptor, but also regulated the intestinal microbiota by enhancing the typical beneficial microbe Akkermansia muciniphila, thereby modulating the colonic microenvironment. These results suggested that TSA had a potential strengthening effect on the resistance of healthy mice to metabolic disorders, which provides a theoretical basis for the utilization of TSA. © 2022 Elsevier Ltd
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英文
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Characterization and modelling of odor-active compounds release behavior from Fu-brick tea during boiling-water extraction by molecular sensory science approach
作者：
Wang, Chao;Xu, Wazhen;Yuan, Yuqi;Zhai, Yuke;Hu, Tengfei;...
期刊：
Food Chemistry: X ,2023年17:100551 ISSN：2590-1575
通讯作者：
Li, Q
作者机构：
[Hu, Tengfei; Yuan, Yuqi; Zhai, Yuke; Wang, Chao; Xu, Wazhen; Liu, Zhonghua; Li, Qin; Huang, Jianan; Li, Q] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Wang, Chao; Liu, Zhonghua; Li, Qin; Huang, Jianan] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Changsha 410128, Hunan, Peoples R China.;[Wang, Chao; Liu, Zhonghua; Li, Qin; Huang, Jianan] Hunan Agr Univ, Coinnovat Ctr Educ Minist Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Li, Q ] H;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.
关键词：
Fu-brick tea;Odor-active compounds;Release behaviors;Sensory analysis;Instrumental analysis;Nonlinear curve fitting analysis
摘要：
The odor-active compounds in Fu-brick tea and their release behaviors during boiling-water were studied. Fifty-one odor-active compounds were identified, and their release behaviors were identified by continuously recovering 16 sections of condensed water combined with sensory, instrumental, and nonlinear curve fitting analysis. The odor intensities of condensed water and the concentrations of odor-active compounds could be significantly fitted (p<0.01) to power-function type curves. Hydrocarbons showed the fastest release rate, while organic acids showed the slowest. The release rates had very little correlation with their concentrations, molecular weights, and boiling points. Most odor-active compounds (≥70%) released need to evaporate more than 24% of the added water during boiling-water extraction. Meanwhile, on the basis of odor activity value (OAV) calculation, the aroma recombination experiments were performed to explore the odor-active compounds that made major contributions to the formation of the aroma profile of each condensed water.
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英文
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A comprehensive review of polyphenol oxidase in tea (Camellia sinensis): Physiological characteristics, oxidation manufacturing, and biosynthesis of functional constituents
作者：
Tang, Meng-ge;Zhang, Sheng;Xiong, Li-gui;Zhou, Jing-hui;Huang, Jian-an;...
期刊：
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY ,2023年22(3):2267-2291 ISSN：1541-4337
通讯作者：
Liu, Zhong-hua(larkin-liu@163.com);Liu, Ai-ling(liuailing@hunau.edu.cn);Zhao, Ai-qing(aqzhao@snnu.edu.cn)
作者机构：
[Huang, Jian-an; Liu, Zhong-hua; Xiong, Li-gui; Zhou, Jing-hui; Zhang, Sheng; Liu, Ai-ling; Tang, Meng-ge] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct Ing, Educ Minist Utilizat Bot Funct Ingredients, Coinnovat Ctr, Changsha, Hunan, Peoples R China.;[Huang, Jian-an; Liu, Zhong-hua; Xiong, Li-gui; Zhou, Jing-hui; Zhang, Sheng; Tang, Meng-ge] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha, Hunan, Peoples R China.;[Liu, Ai-ling] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Zhao, Ai-qing; Tang, Meng-ge] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Shaanxi Engn Lab Food Green Proc & Safety Control, Xian, Shaanxi, Peoples R China.;[Zhao, Ai-qing; Tang, Meng-ge] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Shaanxi Key Lab Hazard Factors Assessment Proc & S, Xian, Shaanxi, Peoples R China.
通讯机构：
[Ai-qing Zhao] S;[Zhong-hua Liu; Ai-ling Liu] N;National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi, China<&wdkj&>National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan, China
关键词：
polyphenol oxidase;PPO catalytic products;tea manufacturing
摘要：
Polyphenol oxidase (PPO) is a metalloenzyme with a type III copper core that is abundant in nature. As one of the most essential enzymes in the tea plant (Camellia sinensis), the further regulation of PPO is critical for enhancing defensive responses, cultivating high-quality germplasm resources of tea plants, and producing tea products that are both functional and sensory qualities. Due to their physiological and pharmacological values, the constituents from the oxidative polymerization of PPO in tea manufacturing may serve as functional foods to prevent and treat chronic non-communicable diseases. However, current knowledge of the utilization of PPO in the tea industry is only available from scattered sources, and a more comprehensive study is required to reveal the relationship between PPO and tea obviously. A more comprehensive review of the role of PPO in tea was reported for the first time, as its classification, catalytic mechanism, and utilization in modulating tea flavors, compositions, and nutrition, along with the relationships between PPO-mediated enzymatic reactions and the formation of functional constituents in tea, and the techniques for the modification and application of PPO based on modern enzymology and synthetic biology are summarized and suggested in this article.
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英文
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Acteoside palliates D-galactose induced cognitive impairment by regulating intestinal homeostasis
作者：
Li, Maiquan;Zhu, Mingzhi;Quan, Wei;Huang, Weisu;Liu, Xia;...
期刊：
Food Chemistry ,2023年421:135978 ISSN：0308-8146
通讯作者：
Liu, ZH;Xiao, X
作者机构：
[Quan, Wei; Li, Maiquan; Zhang, Can; Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Peoples R China.;[Liu, Zhonghua; Li, Maiquan; Zhu, Mingzhi] Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Key Lab Tea Sci Minist Educ, Changsha 410128, Peoples R China.;[Xiao, X; Xiao, Xing] Hunan Prov Peoples Hosp, Changsha 410128, Peoples R China.;[Huang, Weisu] Zhejiang Econ & Trade Polytech, Dept Appl Technol, Hangzhou 310018, Peoples R China.;[Lu, Baiyi] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China.
通讯机构：
[Xiao, X ; Liu, ZH ] H;Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Key Lab Tea Sci Minist Educ, Changsha 410128, Peoples R China.;Hunan Prov Peoples Hosp, Changsha 410128, Peoples R China.
关键词：
Acteoside;Antiaging;Cognitive impairment;Intestinal homeostasis
摘要：
Acteoside, an important phenylethanol glycoside, is the main active component in Osmanthus fragrans flower. Our previous study found that acteoside showed high antiaging effect but its absorption rate was low. We speculated acteoside palliated aging-related cognitive impairment before being absorbed, that was intestinal homeostasis underlie the antiaging effect of acteoside. In this study, acteoside was confirmed to palliate cognitive impairment in D-galactose induced aging mice. Acteoside treatment dramatically reduced oxidative stress, alleviated intestinal inflammation, restored intestinal mucosal barrier, rebuilt gut microbiome structure and upregulated gut microbiome metabolites short-chain fatty acids (SCFAs) and amino acids (AAs). Furthermore, antibiotic treatment revealed that the antiaging ability of acteoside was abolished in microbiota depleted mice, which offered direct evidence for the essential role of gut microbiota in the attenuation of cognitive impairment of acteoside. Together, our study indicated that acteoside palliated cognitive impairment by regulating intestinal homeostasisand acteoside intake might be a promising nutritional intervention in prevention of neurodegenerative diseases. © 2023
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英文
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Characterization of the key odor-active compounds in different aroma types of Fu brick tea using HS-SPME/GC-MSO combined with sensory-directed flavor analysis
作者：
Zheng, Xuexue;Hu, Tengfei;Xie, He;Ou, Xingchang;Huang, Jianan;...
期刊：
Food Chemistry ,2023年426:136527 ISSN：0308-8146
通讯作者：
Wang, Chao;Liu, Zhonghua;Li, Q
作者机构：
[Hu, Tengfei; Xie, He; Liu, Zhonghua; Ou, Xingchang; Wang, Chao; Zheng, Xuexue; Li, Qin; Huang, Jianan; Li, Q; Wang, C] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Ou, Xingchang; Wang, Chao; Zheng, Xuexue; Li, Qin; Huang, Jianan; Li, Q; Wang, C] Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Hu, Tengfei; Xie, He; Liu, Zhonghua; Wang, Chao; Li, Qin; Huang, Jianan; Li, Q; Wang, C] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, ZH; Li, Q ; Wang, C] H;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Changsha 410128, Hunan, Peoples R China.
关键词：
3,4-Dimethoxytoluene (PubChem CID: 68126);Aroma recombination and omission;Benzaldehyde (PubChem CID: 240);Cedrol (PubChem CID: 65575);Citral (PubChem CID: 638011);Different aroma types;Fu brick tea;Key odor-active compounds;Methyl salicylate (PubChem CID: 4133);Nonanal (PubChem CID: 31289);dodecanal (PubChem CID: 8194);geraniol (PubChem CID: 637566);phenylethyl alcohol (PubChem CID: 6054);α-Ionone (PubChem CID: 5282108)
摘要：
Fu brick tea (FBT) is popular for its unique ‘fungal flower’ aroma, however, its key odor-active compounds are essentially unknown. In this study, the odor-active compounds of 'stale-fungal' aroma (CJX), 'fresh-fungal' aroma (QJX), and 'fermentation-fungal' aroma (FJX) types FBT were extracted and examined by headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC–MS) and gas chromatographyolfactometry (GC-O). A total of 43 volatile and 38 odor-active compounds were identified by these methods. Among them, the content of dihydroactindiolide (4596–13189 µg/L), (E)-linalool oxide (2863–6627 µg/L), and benzyl alcohol (4992–6859 µg/L) were highest. Aroma recombination experiments further verified that these odor-active compounds could be simulated the overall aroma profile of FBT successfully. Furthermore, omission experiments confirmed that 15, 20, and 15 key odor-active compounds in CJX, QJX, and FJX FBT, respectively. This study will provide a theoretical basis for comprehensively understanding the formation of characteristic aromas in FBT. © 2023 Elsevier Ltd
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英文
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Lovastatin Production by Wild Eurotium cristatum Isolated from Fuzhuan Brick Tea Produced Using Forest Resources in Auhua
作者：
Li, Taotao;Liu, Zhanjun;Li, Jun;Zheng, Yajun;Liu, Zhonghua;...
期刊：
Forests ,2023年14(7):1409- ISSN：1999-4907
通讯作者：
Liu, ZH;Ling, PX
作者机构：
[Liu, Zhonghua; Li, Taotao] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Key Lab Educ, Minist Tea Sci, Changsha 410128, Peoples R China.;[Li, Jun; Ling, Peixue; Li, Taotao; Zheng, Yajun] Hunan Weichu Furuida Biotechnol LLC Co, Yiyang 413046, Peoples R China.;[Li, Jun; Liu, Zhanjun; Li, Taotao; Zheng, Yajun] Hunan City Univ, Hunan Prov Key Lab Dark Tea & Jin Hua, Yiyang 413000, Peoples R China.;[Ling, Peixue] Shandong Univ, Natl Glyceon Egineering Res Ctr, Jinan 250199, Peoples R China.
通讯机构：
[Ling, PX ; Liu, ZH ] H;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Key Lab Educ, Minist Tea Sci, Changsha 410128, Peoples R China.;Hunan Weichu Furuida Biotechnol LLC Co, Yiyang 413046, Peoples R China.;Shandong Univ, Natl Glyceon Egineering Res Ctr, Jinan 250199, Peoples R China.
关键词：
lovastatin;Eurotium cristatum;statins production;Anhua tea grove;Fuzhuan brick tea
摘要：
Fuzhuan brick tea is a dark tea produced using tea plant leaves, and its quality is strongly affected by Eurotium cristatum. The superior Eurotium cristatum strain could enhance functional components in Fuzhuan brick tea, improving its quality and economic value. To research the lipid-lowering substance of Eurotium cristatum in Fuzhuan brick tea from Anhua country tea production area, several wild-type Eurotium cristatum strains were screened for lovastatin production. Of all 20 wild-type strains, 6 Eurotium cristatum strains could produce lovastatin, and the H20 test strain could produce the highest lovastatin. The organ of lovastatin production was studied, and its yield in sexual propagule (ascospore and ascocarp) was the highest. Some fermentation parameters for lovastatin produced by the H20 strain were researched, and the optimum condition, such as PDA medium containing additional 8% soluble starch, 32 °C, and 120 r/min, are reported. These results are promising for developing one Fuzhuan brick tea with a high lipid-lowering function and expanding the scope of tea plant forest resource application. © 2023 by the authors.
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英文
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Oolong tea of different years protects high-fat diet-fed mice against obesity by regulating lipid metabolism and modulating the gut microbiota
作者：
Fang, Wen-wen;Wang, Kuo-fei;Zhou, Fang;Ou-yang, Jie;Zhang, Zi-ying;...
期刊：
FOOD & FUNCTION ,2023年14(6):2668-2683 ISSN：2042-6496
通讯作者：
Huang, Jian-An(jian7513@hunau.edu.cn);Liu, Zhong-Hua(zhonghua-liu-ms@hunau.edu.cn)
作者机构：
[Fang, Wen-wen; Huang, Jian-an; Liu, Zhong-hua; Wang, Kuo-fei; Zhang, Zi-ying; Ou-yang, Jie; Zeng, Hong-zhe; Liu, Chang-wei; Zhou, Fang] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct Ing, Coinnovat Ctr Educ Minist Utilizat Bot Funct Ingre, Key Lab Tea Sci,Minist Educ,Key Lab Evaluat & Util, Changsha 410128, Peoples R China.
通讯机构：
[Jian-an Huang; Zhong-hua Liu] K;Key Laboratory of Tea Science of Ministry of Education;National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients;Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients;Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, China
摘要：
Long-term stored oolong tea has recently attracted considerable attention concerning its salutary effect. In this study, the anti-obesity effect of different years’ oolong tea on high-fat diet-fed mice was compared. Wuyi rock tea of 2001, 2011, and 2020 were chosen to be the representative samples of oolong tea. The results showed that eight-week administration of 2001 Wuyi rock tea (WRT01), 2011 Wuyi rock tea (WRT11), and 2020 Wuyi rock tea (WRT20) extracts (400 mg per kg per d) significantly decreased the body weight and attenuated the obesity in high-fat diet-fed mice. 2001 and 2011 Wuyi rock teas reduced obesity mainly through regulating lipid metabolism and activating the AMPK/SREBP-1 pathway, downregulating the expression of SREBP-1, FAS, and ACC and upregulating CPT-1a expression; while the 2011 and 2020 Wuyi rock teas by moderating the gut microbiota dysbiosis, reshaping the gut microbiota, and promoting the growth of beneficial bacteria, especially Akkermansia. 2011 Wuyi rock tea was proven to be more effective in reducing body weight gain and liver oxidative stress than the others. Collectively, all three Wuyi rock teas of different years alleviated high-fat diet-induced obesity by regulating lipid metabolism and modulating gut microbiota, whereas the emphasis of their internal mechanism is different with different storage ages. © 2023 The Royal Society of Chemistry.
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Synergistic effects of l-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation
作者：
Xu, Wei;Song, Xianying;Qu, Qingyun;Gong, Zhihua;Xiao, Wenjun
期刊：
FOOD & FUNCTION ,2023年14(4):2059-2073 ISSN：2042-6496
通讯作者：
Gong, Zhihua(gzh041211@163.com);Xiao, Wenjun(xiaowenjun88@sina.com)
作者机构：
[Song, Xianying; Gong, Zhihua; Qu, Qingyun; Xiao, Wenjun; Xu, Wei] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Song, Xianying; Gong, Zhihua; Qu, Qingyun; Xiao, Wenjun; Xu, Wei] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Song, Xianying; Gong, Zhihua; Qu, Qingyun; Xiao, Wenjun; Xu, Wei] Coinnovat Ctr Educ Minist Utilizat Bot Funct Ingre, Changsha, Peoples R China.;[Song, Xianying; Gong, Zhihua; Qu, Qingyun; Xiao, Wenjun; Xu, Wei] Hunan Agr Univ, Hunan Collaborat Innovat Ctr Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhihua Gong; Wenjun Xiao] K;Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, China<&wdkj&>Hunan Agricultural University, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China
摘要：
Ovalbumin (OVA), a commonly consumed food protein, can cause severe allergies and intestinal immune disorders. l-Theanine (LTA) and epigallocatechin gallate (EGCG) regulate intestinal immunity. However, it is unclear whether an LTA and EGCG combined intervention can alleviate OVA allergy (OVA-A) by modulating intestinal-specific immunity, and it is unknown whether there is a synergistic effect between LTA and EGCG. Therefore, we treated BALB/c OVA-sensitized mice with LTA, EGCG, or a combination of both (LTA + EGCG) to investigate the effects of LTA and EGCG on intestinal-specific immunity regulation and underlying mechanisms. Female mice were intraperitoneally injected with OVA to establish OVA-sensitive mouse models. MLEO LTA + EGCG (20 mg kg−1 d−1 LTA + 80 mg kg−1 d−1 EGCG) and HLEO (30 mg kg−1 d−1 LTA + 120 mg kg−1 d−1 EGCG) exerted more beneficial effects on alleviating OVA-A (weight gain, allergy score, jejunum structure, mast cell [MC] degranulation, thymus and spleen indices) than LTA or EGCG alone (p < 0.01). Based on the alleviation of OVA-A by LTA + EGCG, we selected MLEO mice for 16S rDNA, flow cytometry, and western blot analyses. The 16S rDNA results showed that MLEO increased the abundance of Lactobacillaceae, Lachnospiraceae, and Ruminococcaceae, and decreased that of Helicobacteraceae (p < 0.01). The flow cytometry and western blotting results indicated that MLEO reduced the number of dendritic cells available to capture OVA, thereby lowering the Th2 immune response and decreasing the IL-4 and IL-13 levels. Meanwhile, the attenuation of the Th2 immune response inhibits the cross-linking of OVA and FcϵRI, thus reducing MC degranulation and decreasing the serum HIS and mMCPT-1 levels through the FcϵRI/Btk/PLCγ signaling pathway. LTA + EGCG also inhibits the Th2 immune response through the FcϵRI/Lyn/Syk/PI3K/AKT signaling pathway and decreases the serum IL-4 and IL-13 levels. Notably, LTA + EGCG promotes the Treg and Th1 immune responses and inhibits the Th17 immune response, altering the levels of the corresponding cytokines. Therefore, LTA + EGCG can synergistically alleviate OVA-A by regulating intestinal immunity through MC degranulation inhibition. © 2023 The Royal Society of Chemistry.
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L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice
作者：
Liu, Kehong;Liu, Enshuo;Lin, Ling;Hu, Yuan;Yuan, Yong* ;...
期刊：
FOOD & FUNCTION ,2022年13(4):2120-2130 ISSN：2042-6496
通讯作者：
Xiao, Wenjun;Yuan, Yong
作者机构：
[Liu, Kehong; Xiao, Wenjun; Hu, Yuan; Liu, Enshuo; Lin, Ling] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.;[Liu, Kehong; Xiao, Wenjun; Hu, Yuan; Liu, Enshuo; Lin, Ling] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Yuan, Yong] Hunan Tea Grp Co Ltd, Changsha 410128, Peoples R China.
通讯机构：
[Xiao, Wenjun; Yuan, Yong] H;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;Hunan Tea Grp Co Ltd, Changsha 410128, Peoples R China.
摘要：
l-Theanine, an active ingredient in the tea plant (Camellia sinensis) associated with calming, is widely used as a functional ingredient and dietary supplement. In this study, a heat stress mouse model was used to evaluate the anti-heat stress effect of l-theanine and its possible mechanism of action. Mice subjected to heat stress (40 °C) that were administered l-theanine at various doses (100, 200, and 400 mg kg-1 d-1) had reduced oxidative stress and inflammatory factors when l-theanine was administered both long-term and as a preventative treatment. Our l-theanine intervention countered the reduction in growth and feed intake of mice under heat stress and reversed liver and jejunum tissue damage. Moreover, l-theanine countered the increase in inflammatory factors TNF-α, IL-6, and IL-1β and antioxidant enzymes SOD and CAT; it also counteracted GSH-Px inactivation, the upregulation of AST and ALT enzyme activity, and MDA production. The mechanism of action may involve mediation of the P38 signaling pathway, inhibition of MK2 overexpression, and downregulation of p-P65/P65 caused by the overexpression of downstream HSP27. This would inhibit the heat stress-induced imbalance in oxidative stress and inflammatory responses. This journal is © The Royal Society of Chemistry.
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Fuzhuan brick tea polysaccharides serve as a promising candidate for remodeling the gut microbiota from colitis subjects in vitro: Fermentation characteristic and anti-inflammatory activity
作者：
Chen, Guijie;Wang, Mingjia;Zeng, Ziqi;Xie, Minhao;Xu, Weiqi;...
期刊：
Food Chemistry ,2022年391:133203 ISSN：0308-8146
通讯作者：
Zeng, Xiaoxiong(zengxx@njau.edu.cn)
作者机构：
[Chen, Guijie; Xu, Weiqi; Zeng, Ziqi; Peng, Yujia; Wang, Mingjia; Zeng, Xiaoxiong; Sun, Yi; Zhou, Wangting] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;[Xie, Minhao] Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Collaborat Innovat Ctr Modern Grain Circulat & Saf, Nanjing 210023, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Key Lab Minist Educ Tea Sci, Changsha 410128, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Peoples R China.
通讯机构：
[Zeng, X.] K;Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, China
关键词：
Colitis;Fuzhuan brick tea;Gut microbiota;Polysaccharides;Prebiotics;Short-chain fatty acids
摘要：
The purified fraction 3 of polysaccharides from Fuzhuan brick tea (FBTPS-3) could attenuate the colitis and modulate the gut microbiota. However, the relationship between anti-inflammatory effect of FBTPS-3 and the gut microbiota is still unknown. Thus, the anaerobic fermentation in vitro was used to investigate the potential mechanism. FBTPS-3 could be utilized and degraded by gut microbiota from inflammatory bowel disease (IBD) subjects. Furthermore, FBTPS-3 could modulate the composition and structure of IBD gut microbiota toward to that of healthy group. FBTPS-3 showed a superior modulated effect on IBD gut microbiota by increasing Bacteroides and decreasing Escherichia/Shigella. Furthermore, the fermentation solution rather than FBTPS-3 itself showed anti-inflammatory effects on lipopolysaccharide-treated RAW264.7 macrophages, which might be due to the metabolites such as short-chain fatty acids (SCFAs). Thus, FBTPS-3 can be expected as novel prebiotics for treatment of IBD via modulating gut microbiota, and promoting the production of SCFAs. © 2022 Elsevier Ltd
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Impact of prolonged withering on phenolic compounds and antioxidant capability in white tea using LC-MS-based metabolomics and HPLC analysis: Comparison with green tea
作者：
Zhou, Binxing* ;Wang, Zihao;Yin, Peng;Ma, Bingsong;Ma, Cunqiang* ;...
期刊：
Food Chemistry ,2022年368:130855 ISSN：0308-8146
通讯作者：
Zhou, Binxing;Ma, Cunqiang
作者机构：
[Ma, Cunqiang; Zhou, Binxing; Wang, Jiacai; Zhou, BX; Ma, CQ; Ma, Bingsong; Wang, Ziyu; Yin, Dingfang; Xu, Chengcheng] Yunnan Agr Univ, Coll Longrun Pu Erh Tea, Kunming 650201, Yunnan, Peoples R China.;[Wang, Zihao; Yin, Peng] Xinyang Agr & Forestry Univ, Tea Coll, Henan Key Lab Tea Comprehens Utilizat South Henan, Xinyang 464000, Henan, Peoples R China.;[Yin, Peng] Hunan Agr Univ, Coll Hort, Key Lab Tea Sci, Educ Minist, Changsha 410128, Hunan, Peoples R China.;[Xia, Tao] Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Hefei 230036, Peoples R China.
通讯机构：
[Zhou, BX; Ma, CQ] Y;Yunnan Agr Univ, Coll Longrun Pu Erh Tea, Kunming 650201, Yunnan, Peoples R China.
关键词：
(−)-Epigallocatechin gallate (PubChem Compound CID: 65064);1,3,7-Trimethyluric acid (PubChem Compound CID: 79437);Antioxidant activities;Caffeine (PubChem Compound CID: 2519);Flavonoids;Isotheaflavin 3'-gallate (PubChem Compound CID: 135704008);Kaempferol (PubChem Compound CID: 5280863);LC-MS metabonomics;Luteolin (PubChem Compound CID: 5280445);Quercetin (PubChem Compound CID: 5280343);Taxifolin (PubChem Compound CID: 24887336);Theaflavin (PubChem Compound CID: 4263901);Theaflavin 3,3'-digallate (PubChem Compound CID: 136277567);Theaflavins;White tea
摘要：
Contents of 20 bioactive compounds in 12 teas produced in Xinyang Region were determined by high performance liquid chromatography. Ultra-high performance liquid chromatography-quadrupole time of flight-mass spectrometry was developed for untargeted metabolomics analysis. Antioxidant activities were measured by 4 various assays. Those teas could be completely divided into green and white tea through principal component analysis, hierarchical cluster analysis and orthonormal partial least squares-discriminant analysis (R2Y = 0.996 and Q2 = 0.982, respectively). The prolonged withering generated 472 differentiated metabolites between white and green tea, prompted significant decreases (variable importance in the projection > 1.0, p-value < 0.05 and fold change > 1.50) of most catechins and 8 phenolic acids to form 4 theaflavins, and benefited for the accumulation of 17 flavonoids and flavonoid glycosides, 8 flavanone and their derivatives, 20 free amino acids, 12 sugars and 1 purine alkaloid. Additionally, kaempferol and taxifolin contributed to 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability of white tea. © 2021 Elsevier Ltd
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Improved yield of theaflavin-3,3ʹ-digallate from Bacillus megaterium tyrosinase via directed evolution
作者：
Zhou, Jinghui;Liu, Changwei;Zhao, Shimin;Liu, Ya;Zhang, Sheng;...
期刊：
Food Chemistry ,2022年375:131848 ISSN：0308-8146
通讯作者：
Liu, Zhonghua
作者机构：
[Liu, Changwei; Liu, Zhonghua; Zhang, Sheng; Zhou, Jinghui; Huang, Jianan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Peoples R China.;[Liu, Changwei; Liu, Zhonghua; Zhang, Sheng; Zhou, Jinghui; Huang, Jianan] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.;[Liu, Changwei; Liu, Zhonghua; Zhang, Sheng; Zhou, Jinghui; Huang, Jianan] Hunan Agr Univ, Educ Minist Utilizat Bot Funct Ingredients, Coinnovat Ctr, Changsha 410128, Peoples R China.;[Zhao, Qiang; Wang, Fen; Zhao, Shimin; Xu, Gang; Liu, Ya] Hunan Flag Biotechnol Co Ltd, Changsha 410100, Peoples R China.
通讯机构：
[Liu, Zhonghua] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Peoples R China.
关键词：
Directed evolution;Theaflavin-3,3ʹ-digallate;Theaflavins;Tyrosinase
摘要：
Theaflavin-3,3ʹ-digallate (TFDG) in black tea possesses several health benefits. However, low TFDG yields limit its application. Herein, tyrosinases from Bacillus megaterium (Bmtyrc) were used to synthesize TFDG. To improve the catalytic efficiency of tyrosinase, a directed evolution strategy and a high-throughput screening method was employed. Compared with the wild type, mutant Bmtyrc-3 (N205D/D166E/D167G/F197W) showed 6.46 and 4.91-folds higher specific activity and 51.97- and 1.95-folds higher Vmax values towards epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), respectively. Moreover, Bmtyrc-3 displayed significantly enhanced catalytic efficiencies, and the space–time yield of TFDG was 35.35 g L-1d-1. Bmtyrc-3 presents a broader substrate binding area, caused by a mutation (N205D) encompassing the active site. Changes in the potential of the substrate binding site and hydrogen bonds, and the electrostatic effect on the protein surface resulted in an increased activity of the substrates EGCG and ECG. © 2021 Elsevier Ltd
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Research on the Guidance of Youth Labor Education Based on the "combination of Education and Production Labor" Program Based on the Deep Learning Model
作者：
Xiao, Linyu;Liao, Xiaoyi
期刊：
Computational Intelligence and Neuroscience ,2022年2022 ISSN：1687-5265
通讯作者：
Liao, Xiaoyi(liaoxiaoyi@hunau.edu.cn)
作者机构：
[Xiao, Linyu] Hunan Agr Univ, Inst Marxism, Changsha 410128, Hunan, Peoples R China.;[Liao, Xiaoyi] Hunan Agr Univ, Coll Hort, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liao, X.] H;Hunan Agricultural University, Hunan, China
摘要：
At present, there is a lack of research on Marx's idea of "combining education and productive labor"and its guiding significance for youth labor education, and no effective teaching model has been formed. In response to this problem, this study proposes a semi-supervised deep learning model based on u-wordMixup (SD-uwM). When there is a shortage of labeled samples, semi-supervised learning uses a large number of unlabeled samples to solve the problem of labeling bottlenecks. However, since the unlabeled samples and labeled samples come from different fields, there may be quality problems in the unlabeled samples, which makes the generalization ability of the model worse., resulting in a decrease in classification accuracy. The model uses the u-wordMixup method to perform data augmentation on unlabeled samples. Under the constraints of supervised cross-entropy and unsupervised consistency loss, it can improve the quality of unlabeled samples and reduce overfitting. The comparative experimental results on the AGNews, THUCNews, and 20Newsgroups data sets show that the proposed method can improve the generalization ability of the model and also effectively improve the time performance. The study found that the SD-uwM model uses the u-wordMixup method to enhance the unlabeled samples and combines the idea of the Mean Teacher model, which can significantly improve the text classification performance. The SD-uwM model can improve the generalization ability and time performance of the model, respectively, 86.4 ± 1.3 and 90.5 ± 1.3. Therefore, the use of SD-uwM in Marx's program is of great practical significance for the guidance process of youth labor education. © 2022 Linyu Xiao and Xiaoyi Liao.
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英文
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Flavonoid-amyloid fibril hybrid hydrogels for obesity control via the construction of gut microbiota
作者：
Hu, Bing;Li, Min;He, Xiaoqian;Wang, Hongliang;Huang, Jianan;...
期刊：
BIOMATERIALS SCIENCE ,2022年10(13):3597-3611 ISSN：2047-4830
通讯作者：
Hu, Bing(hubing212002@njau.edu.cn);Liu, Zhonghua(zhonghua-liu@hunau.edu.cn)
作者机构：
[Li, Min; Hu, Bing; Wang, Hongliang; He, Xiaoqian] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China.;[Hu, Bing; Liu, Zhonghua; Huang, Jianan] Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Peoples R China.;[Hu, Bing; Liu, Zhonghua; Huang, Jianan] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.;[Mezzenga, Raffaele] Swiss Fed Inst Technol, Dept Hlth Sci & Technol, CH-8092 Zurich, Switzerland.
通讯机构：
[Bing Hu] C;[Zhonghua Liu] K;College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. China<&wdkj&>Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, China<&wdkj&>National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, China<&wdkj&>Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, China<&wdkj&>National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, China
摘要：
Innovative precise clinical approaches to protect humans from the alarming global growth of chronic disease epidemics, such as metabolic syndrome (MetS), are urgently needed. Here, we introduce protein hydrogels developed through the self-assembly of flavonoids and protein amyloid fibrils as a possible approach to mitigate obesity. After oral administration of the hydrogels, high-fat diet (HFD)-induced obesity was significantly prevented in mice, accompanied by downregulation of lipogenesis and pro-inflammatory genes in the liver and adipose tissue and upregulation of lipid metabolism genes. Additionally, gut microbiota dysbiosis caused by HFD-induced obesity was markedly ameliorated. Overexpression of the host intestinal lipid absorption genes CD36 and NFIL3 decreased significantly, while the inhibited expression of the gene encoding the tight junction protein Claudin-1 was reversed. Furthermore, transplantation of the gut microbiota educated by the hydrogels to germ-free mice showed a substantial prevention effect on HFD-induced obesity, accompanied by a distinct microbiota structure that resisted HFD-induced divergence in microbiota structure. The flavonoid-amyloid fibril hydrogels inhibited the core molecular links between gut microbes and host intestinal lipid absorption, enhanced intestinal barrier function and reduced the abundance of bacterial taxa generating pro-inflammatory products, providing a general concept to design edible biomaterials for obesity prevention by targeting host-microbiota crosstalk. © 2022 The Royal Society of Chemistry
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英文
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Dynamic changes in the diversity and function of bacterial community during black tea processing
作者：
Jia, Wen-bao;Zhao, Yi-qiao;Liao, Si-yu;Li, Pin-wu;Zou, Yao;...
期刊：
Food Research International ,2022年161:111856 ISSN：0963-9969
通讯作者：
Zhu, Ming-zhi(mzzhucn@hotmail.com)
作者机构：
[Liao, Si-yu; Chen, Sheng-xiang; Chen, Wei; Jia, Wen-bao; Zou, Yao; Zhao, Yi-qiao; Xu, Wei; Du, Xiao; Li, Pin-wu] Sichuan Agr Univ, Coll Hort, Tea Refining & Innovat Key Lab Sichuan Prov, Chengdu 611130, Peoples R China.;[Zhu, Ming-zhi] Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Key Lab Tea Sci Minist Educ, Changsha 410128, Peoples R China.
通讯机构：
[Ming-zhi Zhu] K;[Wei Xu] C;Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>College of Horticulture, Tea Refining and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
关键词：
Bacterial diversity;Black tea;Core functional bacteria;Functional prediction;Processing;Tea quality-related metabolites
摘要：
Among all types of tea, black tea is produced in the largest amount worldwide, and its consumption is still increasing. Enzymatic fermentation is considered majorly contribute to quality formation of black tea, and the information about the roles of bacterial community in black tea processing is scarce. This study aimed to analyze the dynamic changes in composition, structure, and function of microbial communities during black tea processing and reveal the roles of bacterial community in black tea processing. Results showed that the genera Sphingomonas and Variovorax were dominant throughout the processing of black tea. Prediction function analysis of bacterial community showed that the mean proportions of glucuronoarabinoxylan endo − 1,4 − beta − xylanase, aminopeptidase B, phosphoserine phosphatase, homoserine O-acetyltransferase, glycolysis related enzymes, pyruvate dehydrogenase, tricarboxylic acid cycle related enzymes, and glyoxylate bypass were significantly elevated in the rolling and fermentation stages. The contents of amino acids, soluble sugar, theaflavins, thearubigins, and theabrownins increased greatly during the rolling and fermentation processes. Redundancy and Pearson's correlation analyses showed that the relative abundance of bacteria was closely related to the contents of water extract, tea polyphenols, epigallocatechin, epicatechin gallate, catechin gallate, thearubigins, theaflavins, and theabrownins. Overall, the findings provided new insights into the variation of bacterial community during black tea processing and improved our understanding of the core functional bacteria involved in black tea processing. © 2022 Elsevier Ltd
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Characterization of key aroma compounds and relationship between aroma compounds and sensory attributes in different aroma types of Fu brick tea
作者：
Zheng Xuexue;Hong Xin;Jin Youlan;Wang Chao;Liu Zhonghua;...
期刊：
Food Chemistry: X ,2022年13:100248 ISSN：2590-1575
通讯作者：
Huang Jianan;Li, Q
作者机构：
[Liu Zhonghua; Huang Jianan; Li Qin; Hong Xin; Wang Chao; Zheng Xuexue; Jin Youlan; Li, Q] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Liu Zhonghua; Li Qin; Hong Xin; Huang Jianan; Zheng Xuexue] Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Liu Zhonghua; Li Qin; Jin Youlan] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Huang, JA; Li, Q ] H;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.
关键词：
Fu brick tea;Different aroma types;Key aroma compounds;Sensory attributes;Multivariate statistical analysis
摘要：
Aroma is one of the most important sensory properties of tea. Floral-fungal aroma type, ripe-fungal aroma type and fresh-fungal aroma type were the main aroma types of Fu brick tea by QDA. A total of 112 volatile compounds were identified and quantified in tea samples by HS-SPME/GC–MS analysis. Ten voaltiles in floral-fungal aroma type, eleven voaltiles in ripe-fungal aroma type, and eighteen voaltiles in fresh-fungal aroma type were identified as key aroma compounds for the aroma characteristics formation in three aroma types of Fu brick tea. In addition, PLS analysis revealed that 3,4-dehydro-β-ionone, dihydro-β-ionone, (+)-carotol and linalool oxide Ⅱ were the key contributors to the ‘floral and fruity’ attribute, α-terpineol contributed to ‘woody’ and ‘stale’ attributes, and thirteen aroma compounds related to ‘green’ attribute. Taken together, these findings will provide new insights into the formation mechanism of different aroma characteristics in Fu brick tea. © 2022 The Author(s)
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英文
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The metabolic regulation of Fuzhuan brick tea in high-fat diet-induced obese mice and the potential contribution of gut microbiota
作者：
Liu, Dongmin;Wang, Jianhui;Zeng, Hongliang;Zhou, Fang;Wen, Beibei;...
期刊：
FOOD & FUNCTION ,2022年13(1):356-374 ISSN：2042-6496
通讯作者：
Huang, Jianan;Liu, Zhonghua
作者机构：
[Liu, Dongmin; Wang, Jianhui] Changsha Univ Sci & Technol, Changsha 410114, Peoples R China.;[Wen, Beibei; Zhang, Xiangna; Wang, Jianhui; Liu, Zhonghua; Liu, Dongmin; Luo, Yong; Wu, Wenliang; Huang, Jianan; Zhou, Fang] Hunan Agr Univ, Key Lab, Minist Educ Tea Sci, Changsha 410128, Peoples R China.;[Wen, Beibei; Zhang, Xiangna; Wang, Jianhui; Liu, Zhonghua; Liu, Dongmin; Luo, Yong; Wu, Wenliang; Huang, Jianan; Zhou, Fang] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.;[Zeng, Hongliang] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Huang, JA; Liu, ZH] H;[Huang, JA; Liu, ZH] N;Hunan Agr Univ, Key Lab, Minist Educ Tea Sci, Changsha 410128, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.
摘要：
This study investigated the metabolic effects of Fuzhuan brick tea (FBT) in high-fat diet (HFD)-induced obese mice and the potential contribution of gut microbiota. The results showed that FBT ameliorated the HFD-induced glycerophospholipid metabolic aberrance, specifically increased the serum levels of phosphatidylcholines (PCs), lysophosphatidylcholines (LysoPCs), and the ratio of PC to phosphatidylethanolamines (PE). Besides, FBT increased the serum level of gut microbiota-derived aryl hydrocarbon receptor (AhR) ligand, 3-indole propionic acid, as well as the relative abundance of intestinal AhR-ligand producing bacteria such as Clostridiaceae, Bacteroidales_S24-7_group, and Lactobacillaceae. However, the metabolic benefits of FBT were weakened when the gut microbiota were depleted by antibiotic treatment, thereby suggesting that gut microbiota was required for FBT to regulate glycerophospholipid metabolism. Indeed, the metabolites regulated by FBT were significantly correlated with the AhR-ligand producing bacteria. The KEGG pathway enrichment analysis and expressions of AhR target genes indicated that FBT would improve the glycerophospholipid metabolism via the AhR-Pemt signal axis, in which the gut microbiota and their metabolites played pivotal mediators. Overall, FBT could be a functional beverage to improve HFD-induced metabolic disorders in a gut microbiota dependent manner. © 2022 The Royal Society of Chemistry.
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