通讯机构：
[Bai, LY; Bai, LY ; Pan, L ] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;Hunan Agr Biotechnol Res Inst, Hunan Acad Agr Sci, Changsha, Peoples R China.
关键词：
Acetolactate synthase (ALS);Mesosulfuron-methyl;gene mutation;homology modelling and docking;Bromus japonicus
摘要：
Introduction Bromus japonicus is one of the most notorious agricultural weeds in China. The long-term use of ALS-inhibiting herbicides has led to rapid evolution of herbicide resistance in B. japonicus. B. japonicus population (BJ-R) surviving mesosulfuron-methyl treatment was collected from wheatland. Here, we aimed to confirm the resistance mechanisms in this putative resistant population. Methods The dose-reponse tests were used to test the resistance level of the B. japonicus to ALS-inhibiting herbicides. Pretreatment with P450 and GST inhibitors and GST activity assays were used to determine whether P450 or GST was involved in the resistance of the BJ-R population. Sanger sequencing was used to analyse the ALS mutation of the BJ-R population. RT-qPCR was used to confirm the the expression levels of the ALS gene in mesosulfuron-methyl -resistant (BJ-R) and-susceptible (BJ-S) B. japonicus. An in vitro ALS activity assay was used to determine the ALS activity of the BJ-R and BJ-S populations. Homology modelling and docking were used to determine the binding energy of the BJ-R and BJ-S populations with ALS-inhibiting herbicides. Results B. japonicus population (BJ-R) was confirmed to be 454- and 2.7-fold resistant to the SU herbicides mesosulfuron-methyl and nicosulfuron, and 7.3-, 2.3-, 1.1- and 10.8-fold resistant to the IMI herbicide imazamox, the TP herbicide penoxsulam, the PTB herbicide pyribenzoxim and the SCT herbicide flucarbazone-sodium, respectively, compared with its susceptible counterpart (BJ-S). Neither a P450 inhibitor nor a GST inhibitor could reverse the level of resistance to mesosulfuron-methyl in BJ-R. In addition, no significant differences in GST activity were found between the BJ-R and BJ-S. ALS gene sequencing revealed a Pro-197-Thr mutation in BJ-R, and the gene expression had no significant differences between the BJ-R and BJ-S. The ALS activity of BJ-R was 106-fold more tolerant to mesosulfuron-methyl than that of BJ-S. Molecular docking showed that the binding energy of the ALS active site and mesosulfuron-methyl was changed from -6.67 to -4.57 kcal mol(-1) due to the mutation at position 197. Discussion These results suggested that the Pro-197-Thr mutation was the main reason for the high resistance level of BJ-R to mesosulfuron-methyl. Unlike previous reports of the cross-resistance pattern conferred by this mutation, we firstly documented that the Pro-197-Thr mutation confers broad cross-resistance spectrums to ALS-inhibiting herbicides in B. japonicus.
作者机构：
[Tangyu Yuan] Yantai Academy of Agricultural Science, No. 26 Gangcheng West Street, Fushan District, Yantai City 265500, China;College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;[Ying Liu; Yingjie Zhao] State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China;Authors to whom correspondence should be addressed.;These authors contributed equally to this work.
通讯机构：
[Wei Li; Jiaqian Liu] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
摘要：
Glycosylation, a dynamic modification prevalent in viruses and higher eukaryotes, is principally regulated by uridine diphosphate (UDP)-glycosyltransferases (UGTs) in plants. Although UGTs are involved in plant defense responses, their responses to most pathogens, especially plant viruses, remain unclear. Here, we aimed to identify UGTs in the whole genome of Nicotiana benthamiana (N. benthamiana) and to analyze their function in Chinese wheat mosaic virus (CWMV) infection. A total of 147 NbUGTs were identified in N. benthamiana. To conduct a phylogenetic analysis, the UGT protein sequences of N. benthamiana and Arabidopsis thaliana were aligned. The gene structure and conserved motifs of the UGTs were also analyzed. Additionally, the physicochemical properties and predictable subcellular localization were examined in detail. Analysis of cis-acting elements in the putative promoter revealed that NbUGTs were involved in temperature, defense, and hormone responses. The expression levels of 20 NbUGTs containing defense-related cis-acting elements were assessed in CWMV-infected N. benthamiana, revealing a significant upregulation of 8 NbUGTs. Subcellular localization analysis of three NbUGTs (NbUGT12, NbUGT16 and NbUGT17) revealed their predominant localization in the cytoplasm of N. benthamiana leaves, and NbUGT12 was also distributed in the chloroplasts. CWMV infection did not alter the subcellular localization of NbUGT12, NbUGT16, and NbUGT17. Transient overexpression of NbUGT12, NbUGT16, and NbUGT17 enhanced CWMV infection, whereas the knockdown of NbUGT12, NbUGT16 and NbUGT17 inhibited CWMV infection in N. benthamiana. These NbUGTs could serve as potential susceptibility genes to facilitate CWMV infection. Overall, the findings throw light on the evolution and function of NbUGTs.
作者机构：
College of Horticulture, Hunan Agricultural University , Changsha, China , 410128;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education , Changsha, China , 410128;Key Laboratory for Vegetable Biology of Hunan Province , Changsha, China , 410128;[Jiahui Zhang; Yunsheng Wang] Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests, Hunan Agricultural University , Changsha, China , 410128;[Qiuyun Wu; Shuxiang Mao; Huiping Huang; Juan Liu; Xuan Chen; Linghui Hou; Yuxiao Tian; Junwei Wang; Ke Huang] College of Horticulture, Hunan Agricultural University , Changsha, China , 410128<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education , Changsha, China , 410128<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province , Changsha, China , 410128
通讯机构：
[Junwei Wang; Ke Huang] C;[Yunsheng Wang] H;College of Horticulture, Hunan Agricultural University , Changsha, China , 410128<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education , Changsha, China , 410128<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province , Changsha, China , 410128<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests, Hunan Agricultural University , Changsha, China , 410128
摘要：
Broccoli (Brassica oleracea var. italica Plenck) is an important vegetable crop, as it is rich in health-beneficial glucosinolates (GSLs). However, the genetic basis of the GSL diversity in Brassicaceae remains unclear. Here, we report a chromosome-level genome assembly of broccoli generated using PacBio HiFi reads and Hi-C technology. The final genome assembly is 613.79 Mb in size, with a contig N50 of 14.70 Mb. The GSL profile and content analysis of different Brassicas oleracea varieties, combined with a phylogenetic tree analysis, sequence alignment, and the construction of a three-dimensional model of the methylthioalkylmalate synthase 1 (MAM1) protein, which revealed that the gene copy number and amino acid sequence variation both contributed to the diversity of GSL biosynthesis in Brassica oleracea. The overexpression of BoMAM1 (BolI0108790) in broccoli resulted in high accumulation and a high ratio of C4-GSLs, demonstrating that BoMAM1 is the key enzyme in C4-GSLs biosynthesis. These results provide valuable insights for future genetic studies and nutritive component applications of Brassica crops.
通讯机构：
[Youzhi Li] H;Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Chilo suppressalis is one of the most damaging rice pests in China's rice-growing regions. Chemical pesticides are the primary method for pest control; the excessive use of insecticides has resulted in pesticide resistance. C. suppressalis is highly susceptible to cyproflanilide, a novel pesticide with high efficacy. However, the acute toxicity and detoxification mechanisms remain unclear. We carried out a bioassay experiment with C. suppressalis larvae and found that the LD10, LD30 and LD50 of cyproflanilide for 3rd instar larvae was 1.7 ng/per larvae, 6.62 ng/per larvae and 16.92 ng/per larvae, respectively. Moreover, our field trial results showed that cyproflanilide had a 91.24% control efficiency against C. suppressalis. We investigated the effect of cyproflanilide (LD30) treatment on the transcriptome profiles of C. suppressalis larvae and found that 483 genes were up-regulated and 305 genes were down-regulated in response to cyproflanilide exposure, with significantly higher CYP4G90 and CYP4AU10 expression in the treatment group. The RNA interference knockdown of CYP4G90 and CYP4AU10 increased mortality by 20% and 18%, respectively, compared to the control. Our results indicate that cyproflanilide has effective insecticidal toxicological activity, and that the CYP4G90 and CYP4AU10 genes are involved in detoxification metabolism. These findings provide an insight into the toxicological basis of cyproflanilide and the means to develop efficient resistance management tools for C. suppressalis.
通讯机构：
[Guo-Hua Huang] C;College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road, Furong District, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
通讯机构：
[Huan Yu; Gong Chen; Huan Yu Huan Yu Huan Yu; Gong Chen Gong Chen Gong Chen] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, People's Republic of China<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, People's Republic of China
摘要：
In recent years, carbon-based materials catalyzing peroxymonosulfate (PMS) for green degradation of persistent or-ganic pollutants have attracted increasing attention. However, PMS activation by hydrochar composite (e.g. hydrochar-montomorillonite) has rarely been investigated. Herein, a simple preparation, low-cost and eco-friendly catalyst of hydrochar-montmorillonite composite (HC-Mt) was prepared to firstly catalyze PMS for the degradation of dicamba (DIC). The as-prepared HC-Mt showed a remarkably better catalyzing performance for PMS than pure hydrochar (HC) due to its good physicochemical characteristics and abundant oxygen-containing groups. Further-more, the electron spin resonance (ESR) and quenching tests revealed that active species such as SO4 center dot-, center dot OH and O2 center dot- all participated in the degradation process. DIC sites on C6, Cl 10, and O15 exhibited higher reactivity according to the density functional theory (DFT) calculation, which were easily attacked by active species. The DIC degradation mainly occurred via hydroxyl substitution, decarboxylation, oxidation and ring-cleavage and finally most of the inter-mediates were mineralized into CO2 and H2O. Finally, the phytotoxicity assessment was measured by the germination growth situation of tobacco and mung beans in the presence of DIC (with or without treatment by HC-Mt/PMS). The result showed that HC-Mt/PMS could significantly reduce the phytotoxicity of DIC to crops, suggesting that catalyzing PMS using HC-Mt was environmentally friendly. Therefore, this work did not only provide a novel catalyzing PMS strategy using hydrochar composite for wastewater treatment, but also give a new idea for herbicide phytotoxicity management.
通讯机构：
[Lianyang Bai; Lang Pan] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Plant Protection, Hunan Agricultural University, No. 1, Nongda Road, Furong District, Changsha 410128, China
摘要：
Asia minor bluegrass (Polypogon fugax) is a common and problematic weed throughout China. P. fugax that is often controlled by acetyl-CoA carboxylase (ACCase) inhibitors in canola fields. Herein, we confirmed a P. fugax population (R) showing resistance to all ACCase inhibitors tested with resistance indexes ranging from 5.4–18.4. We further investigated the resistance mechanisms of this R population. Molecular analyses revealed that an amino acid mutation (Asp-2078-Gly) was present in the R population by comparing ACCase gene sequences of the sensitive population (S). In addition, differences in susceptibility between the R and S population were unlikely to be related to herbicide metabolism. Furthermore, a new derived cleaved amplified polymorphic sequence (dCAPS) method was developed for detecting the Asp-2078-Gly mutation in P. fugax efficiently. We found that 93.75% of plants in the R population carried the Asp-2078-Gly mutation, and all the herbicide-resistant phenotype of this R population is inseparable from this mutation. This is the first report of cross resistance to ACCase inhibitors conferred by the Asp-2078-Gly target-site mutation in P. fugax. The research suggested the urgent need to improve the diversity of weed management practices to prevent the widespread evolution of herbicide resistance in P. fugax in China.
通讯机构：
[Lin Qiu; Lin Qiu Lin Qiu Lin Qiu] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China
关键词：
Surface ion imprinting;support;heavy metal ions;adsorption
摘要：
Surface ion-imprinting technology (SIIT) is a novel and effective alternative to conventional methods of preparing ion-imprinted polymers. SIIT involves the coating of support materials with a polymeric layer that selectively binds with imprinted metal ions, particularly heavy metal ions. Given its specificity, predictability, and stability, SIIT has been quickly adopted in ion detection, separation, enrichment, and sensing. Here, building on the latest advances in this emerging new technology, we carried out a systematic review to summarize the 1) principles and basic components of surface ion-imprinted polymers (SIIPs); 2) preparations, properties, advantages, and disadvantages of three types of SIIP supporting materials, including inorganic (minerals, carbonaceous materials, metal oxides), organic, and composite (organic/organic, organic/inorganic, inorganic/inorganic) supports; 3) current applications of SIIT, and 4) future challenges and opportunities related to SIIT. Finally, perspectives and future research are discussed to address the pressing need for the development of SIIT for heavy metal ion treatment.
期刊：
Zoological Journal of the Linnean Society,2023年198(2):476-493 ISSN：0024-4082
通讯作者：
Xing Wang<&wdkj&>Guo-Hua Huang
作者机构：
[Liao, Cheng-Qing; Chen, Qi; Huang, Guo-Hua; Chen, Lu; Wang, Xing] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Liao, Cheng-Qing; Chen, Qi; Huang, Guo-Hua; Chen, Lu] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.;[Yagi, Sadahisa; Hirowatari, Toshiya] Kyushu Univ, Fac Agr, Entomol Lab, 744 Motooka,Nishi Ku, Fukuoka 8190395, Japan.;[Wang, Xing] Qiongtai Normal Univ, Coll Sci, Haikou 571100, Hainan, Peoples R China.;[Wang, Xing] South China Agr Univ, Dept Entomol, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Xing Wang; Guo-Hua Huang] C;College of Plant Protection, Hunan Agricultural University , Changsha, Hunan 410128 , China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University , Nongda Road 1, Furong District, Changsha, Hunan 410128 , China<&wdkj&>College of Plant Protection, Hunan Agricultural University , Changsha, Hunan 410128 , China<&wdkj&>College of Science, Qiongtai Normal University , Haikou, Hainan 571100 , China
摘要：
As one of the largest single-radiation sources of phytophagous insects, the higher level phylogeny of lepidopteran lineages has been debated for decades, especially the early divergences of nonditrysia. Here, we use 74 mitochondrial genomes representing seven nonditrysian superfamilies, including the newly sequenced mitochondrial genomes of eight families and 30 species. Four datasets were constructed and used for a phylogenetic analysis based on maximum likelihood (ML) and Bayesian inference (BI) methods. The results reveal that (1) nonditrysian lineages presented typically nested relationships with the family Micropterigidae as the sister-group to the remaining Lepidoptera, (2) monophyly of major clades in Lepidoptera, including Glossata, Heteroneura, Eulepidoptera and Ditrysia, was strongly supported, and (3) Neopseustoidea formed the sister of the remaining Glossata. Divergence-time analysis estimated that Lepidoptera originated c. 222 Mya during the Middle Triassic and that most superfamilies/families of nonditrysia originated from the Early Cretaceous to the Early Palaeogene, which largely coincided with the radiation of angiosperms. Additionally, three novel gene rearrangements in the gene cluster 'MIQ' and 'TP' were found in nonditrysian groups. Further mitochondrial genome data will help us to better understand the evolution of early nonditrysian lineages.
摘要：
Rice blast is a globally devastating fungal disease that affects the production of rice (Oryza sativa), and the screening of excellent biocontrol strains is an important direction for the biological control of the fungus that causes rice blast disease (Magnaporthe oryzae). The objectives were to obtain strains that were highly antagonistic to rice blast, analyze the genetic information of the antagonistic bacterium YN-917, and explore the resources of its antagonistic gene cluster. The antagonistic bacteria were isolated and identified by the plate confrontation method, morphological observation, physiological and biochemical identification, and molecular biology methods. In addition, the strains were subjected to whole-genome sequencing, and their sequences were analyzed. Strain YN-917 was screened from healthy rice plants of the variety Xiangzaoxian 24, which is susceptible to rice blast, and it inhibited M. oryzae by 72.63% +/- 1.30%. Additionally, the strain had different degrees of inhibitory effects on various plant pathogenic fungi and was highly resistant to stress. The morphological observation, analysis of physiological and biochemical features, 16S rRNA homology analysis, and wholegenome sequencing analysis revealed that the strain YN-917 was Bacillus cereus (GenBank No.: PRJNA687285). The total length of its whole genome was 5326162 bp, and its average G + C content was 35.37%. It was composed of one circular chromosome and one endoplasmic plasmid. There were 5483 genes encoded on average. They included 105 tRNA genes, 42 self-replicating RNA (sRNA), 178 tandem repeat sequences, three prophages, and nine genomic islands. The prediction of antagonistic gene cluster demonstrated that the genome sequence of YN-917 had six secondary metabolic gene biosynthetic clusters, including those for bacteriocins, siderophores, non-ribosomal peptide synthetases (NRPs), and terpene. This study provides a theoretical basis to further explore microbial resources and their metabolic gene clusters for agricultural biological control.
作者机构：
[Huan Yu; Hong Chen; Ni Li; Hua-Yan Xiao; Gong Chen; Guo-Hua Huang] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China;Agriculture and Rural Bureau of Xinhuang Dong Autonomous County, Huaihua, Hunan, China;[Chang-Jin Yang] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Agriculture and Rural Bureau of Xinhuang Dong Autonomous County, Huaihua, Hunan, China
通讯机构：
[Gong Chen; Guo-Hua Huang] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China
关键词：
Heliothis virescens ascovirus 3h (HvAV-3h);ascovirus;insect virus;pathogenicity;structural protein
摘要：
Different pathogenic processes of a virus in different hosts are related to the host individual differences, which makes the virus undergoes different survival pressures. Here, we found that the virions of an insect virus, Heliothis virescens ascovirus 3h (HvAV-3h), had different protein composition when they were purified from different host larval species. These "adaptive changes" of the virions were analyzed in detail in this study, which mainly included the differences of the protein composition of virions and the differences in affinity between virions and different host proteins. The results of this study revealed the flexible changes of viruses to help themselves adapt to different hosts. Also, these interesting findings can provide new insights to improve our understanding of virus adaptability and virulence differentiation caused by the adaptation process.
期刊：
Frontiers in Sustainable Food Systems,2023年7:1261745 ISSN：2571-581X
作者机构：
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, China;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, China;State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of Sciences, China;College of Plant Protection, Hunan Agricultural University, China
关键词：
Pest Control;Pollination;biological control;bioactive volatiles;Beneficial nematodes
摘要：
of organisms play critical roles in maintaining the balance of ecosystems and influencing agricultural productivity. Crops, Microbes, bioactive volatiles, nematodes, natural enemy insects, and native pollinators are all key players that impact pests and diseases, biological control, and pollination. Each of these organisms contributes to the intricate web of interactions that shape our environment and influence food production. Exploring the intricate relationships between microbial agents, bioactive volatiles, nematodes, insect-mediated biological control, and pollinators offers a wealth of potential in pest and disease management, promoting sustainable agriculture, and maintaining ecosystem health. In this Research Topic we explore research that harnesses these natural interactions to create more resilient and environmentally friendly approaches to meeting our agricultural and ecological challenges.Microbes such as bacteria and fungi hold immense potential in controlling pests and diseases (da Silva Folli-Pereira et al., 2022). These strains produce natural substances that can act as deterrents, toxins, or even attractants for harmful insects or pathogens. Harnessing these microbial properties offers a sustainable and environmentally friendly alternative to traditional chemical pesticides, reducing the dependence on synthetic compounds and minimizing negative impacts on ecosystems.Rhodopseudomonas palustris belongs to Photosynthetic bacteria, which can decompose various ...
通讯机构：
[Ding, Z ] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;Hunan Prov Engn & Technol Res Ctr Biopesticide & F, Changsha 410128, Peoples R China.
关键词：
Meloidogyne graminicola;population density;agroecosystem;soil moisture;root gall
摘要：
The rice root-knot nematode Meloidogyne graminicola is increasingly widely distributed in China and has had a severe incidence in Hunan Province. It is thus necessary to investigate its population dynamics in paddy fields. This study was conducted to ascertain the effect of direct-seeded rice agroecosystems on the population dynamics of M. graminicola and root gall development in rice. The results indicated that the population density of M. graminicola in soil was markedly influenced by the agroecosystem, rainfall and temperature. The population density of M. graminicola J2, and eggs in the soil and root galls, were significantly larger in the dry aerobic rice agroecosystem and in the rain-fed upland agroecosystem than in the lowland double-rice cropping sequence agroecosystem. As it can affect soil moisture rainfall was the key factor affecting the density of nematodes in both the rain-fed upland agroecosystem and the dry aerobic rice agroecosystem. Field flooding was still an effective way to reduce the population density of M. graminicola. In addition, we observed that M. graminicola can lay eggs outside rice roots under laboratory conditions. Therefore, we propose a hypothesis that M. graminicola lays egg masses within roots when the soil moisture is high, but lays eggs outside when the soil moisture is suitable. By clarifying the population dynamics of M. graminicola in different types of direct-seeded rice agroecosystems, this study is conducive to controlling rice root-knot nematodes.
