摘要：
本文运用RT-PCR方法克隆了黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)湖南邵阳株系的基因组MP(CGMMV-HuNSY movement protein)片段,测序并进行了分析。结果显示,片段全长共有803bp(KC684977),编码由264个氨基酸组成的蛋白质,推测分子量约28.87kD,理论等电点pI为9.06,ProtParam预测显示为不稳定蛋白,与已报道的辽宁分离物病毒的MP作比较,核苷酸相似性为99.6%,氨基酸相似性为98.9%;湖南邵阳株系CGMMVMP蛋白无高度卷曲螺旋部位,有跨膜结构区域,该部位表现为疏水性,可能为蛋白互作位点;磷酸化位点均匀分布于整个多肽链中,存在5个主要的B细胞抗原表位预测位点;对烟草花叶病毒属病毒MP氨基酸序列进行了motif查找,发现了该属病毒氨基酸序列的3个保守区段,还进行了密码子偏向性分析。此外,发现了1个酰胺化位点和1个依赖于cAMP和cGMP的蛋白激酶磷酸化位点,可能与病毒的侵染机制有关。
摘要：
Southern rice black-streaked dwarf virus (SRBSDV) is a recently described member of the genus Fijivirus, family Reoviridae. The roles of the proteins encoded by the SRBSDV genome have rarely been studied. In a yeast two-hybrid (YTH) assay in which SRBSDV P6, a putatively multifunctional protein, was used as bait and an SRBSDV cDNA library was used as prey, there was a strong interaction between the P6 and P5-1 proteins. The interaction was confirmed by bimolecular fluorescence complement (BiFC) assay in plant cells. YTH analysis using truncated mutants showed that the N-terminal region (amino acids 9-231) of P5-1 is necessary for binding P5-1 to P6 and that the N-terminal fragment (amino acids 1-93) of P6 is necessary for its interaction with P5-1. SRBSDV P5-1 formed granules positioned at the cell periphery in Nicotiana benthamiana leaves; P6 was present in both the cytoplasm and the nucleus and formed punctate bodies associated with the cell periphery. Immunogold labeling showed that both P6 and P5-1 localized within viroplasms in infected cells of rice plants. These results suggest that the interaction between P5-1 and P6 of SRBSDV may be involved in the formation of viroplasms.
作者机构：
[DING Zhong; Wang Shuinan; NAMPHUENG Janthathang] College of Plant Protection, Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests, Hunan Agricultural University, Changsha 410128, China;[Peng Deliang; Huang Wenkun] The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, Beijing 100193, China
会议名称：
第四届国际禾谷类线虫病害学术研讨会(The 4th International Cereal Nematode Initiative Workshop)
会议时间：
2013-8-22
会议地点：
北京
会议主办单位：
中国植物病理学会
会议论文集名称：
第四届国际禾谷类线虫病害学术研讨会(The 4th International Cereal Nematode Initiative Workshop)论文集
摘要：
Weeds are important in the ecology of field crops, and when crops are harvested, weeds often become the main hosts for plant viruses and their insect vectors. Few studies, however, have examined the relationships between plant viruses, vectors, and weeds. Here, we investigated how infection of the weed Datura stramonium L. by tomato yellow leaf curl virus (TYLCV) affects the host preference and performance of the TYLCV vector, Bemisia tabaci (Gennadius) Q. The results of a choice experiment indicated that B. tabaci Q preferentially settled and oviposited on TYLCV-infected plants rather than on healthy plants. In addition, B. tabaci Q performed better on TYLCV-infected plants than on healthy plants. These results demonstrate that TYLCV is indirectly mutualistic to B. tabaci Q. The mutually beneficial interaction between TYLCV and B. tabaci Q may help explain the concurrent outbreaks of TYLCV and B. tabaci Q in China.
作者机构：
[陈军; 靳亚峰; 张裕平] Institute of Allied Chemistry, Henan Institute of Science and Technology, Xinxiang 453003, China;[柏连阳] Pesticide Research Institute, Hunan Agricultural University, Changsha 410128, China;[Chen J.; 刘润强] Institute of Allied Chemistry, Henan Institute of Science and Technology, Xinxiang 453003, China, Pesticide Research Institute, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Zhang, Y.] I;Institute of Allied Chemistry, Henan Institute of Science and Technology, China
摘要：
Molecularly imprinted polymer (MIP) was synthesized by bulk polymerization, using olivetol as tem- plate molecule, methyl acrylic acid (MAA) as monomer, ethylene glycol dimethacrylate (EDMA) as crosslink- er, toluene and dodecanol as solvents. The resulted MIP was characterized by the equilibrium binding experi- ments, scanning electron microscope (SEM) and Fourier transform infrared spectrometer (FTIR). The poly- mer was then applied to solid phase extraction (SPE) of olivetol from spiked wheat bran samples. From the equilibrium binding experiments, it was showed that MIP had a better recognizability for the template molecule. Scatchard analysis showed that MIP had specific adsorption to olivetol with two classes of binding sites. The high and low binding sites dissociation constants were 0. 021 and 1. 002 mmol/L. The corresponding maximum binding capacities were 18.74 and 135.9 μmol/g, respectively. Under the optimum condition of SPE, the recoveries of olivetol on MIP cartridge were in the range of 97. 896-98. 896. The relative standard deviations (RSDs) were 2. 896-4. 296. The linearity range was between 0. 1 and 100 mg/L. The limit of detection (S/JV = 3) was 0. 062 mg/L. MIP cartridge showed stronger selectivity, higher recovery and purified the sample more drastically compared with non-imprinted polymer (NIP) cartridge and commercial poly (styrene/divinyl- benzene) (PLS) cartridge.
作者机构：
[S.W.Wang; B.D.Gao] Hunan University Key Laboratory for Control and Utilization of Plant Diseases,Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,College of Bio-Safety Science & Technology,Hunan Agricultural University
会议名称：
第十届国际植物病理学大会暨中国植物病理学会2013年学术年会
会议时间：
2013-08-25
会议地点：
中国北京
会议论文集名称：
Abstracts of ICPP 2013 10th International Congress of Plant Pathology
摘要：
<正>A new rice disease was found in Fenghuang county, Hunan province,in August 2011.Diseased rice plants showed stem-node rot from the top node to lower node, resulting dead head symptom but leaf sheaths intact, which is unique on rice.In order to find out the causal agent,we used microscope check,microbe isolation, pathogenicity test and molecular identification.Color-
摘要：
Background Rice-duck cultivation is the essence of Chinese traditional agriculture. A scientific assessment of the mechanism and its capacity is of theoretical significance and practical value in improving modern agricultural technology. Results The duck's secretions, excreta and their treading, pecking and predation decrease the occurrence of plant diseases, pests and weeds, enrich species diversity and improve the field environment. The rice-duck intergrowth system effectively prevents rice planthoppers and rice leafhoppers. The control effects can be up to 98.47% and 100% respectively; it also has effects on the control of Chilo suppressalis, Tryporyza incertulas and the rice leafrollers. Notable control results are found on sheath blight, while the effects on other diseases are about 50%. Harm from weeds is placed under primary control; prevention of weeds is sequenced by broadleaf weeds>sedge weeds>Gramineae weeds. Contents of soil organic matter, N, P and K are improved by the system; nutrient utilization is accelerated, resulting in decreased fertilizer application. Greenhouse gas emissions are reduced by 1-2% and duck fodder is saved in this system. There is also an obvious economic benefit. Conclusion Compared to conventional rice cultivation, rice-duck cultivation shows great benefits to ecologic cost and economic income. (c) 2013 The Authors. Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry
