摘要：
China, being a major agricultural nation, employs aerobic composting as an efficient approach to handle agricultural solid waste. Nevertheless, the composting process is often accompanied by greenhouse gas emissions, which are known contributors to global warming. Therefore, it is urgent to control the formation and emission of greenhouse gases from composting. This study provides a comprehensive analysis of the mechanisms underlying the production of nitrous oxide, methane, and carbon dioxide during the composting process of agricultural wastes. Additionally, it proposes an overview of the variables that affect greenhouse gas emissions, including the types of agricultural wastes (straw, livestock manure), the specifications for compost (pile size, aeration). The key factors of greenhouse gas emissions during composting process like physicochemical parameters, additives, and specific composting techniques (reuse of mature compost products, ultra-high-temperature composting, and electric-field-assisted composting) are summarized. Finally, it suggests directions and perspectives for future research. This study establishes a theoretical foundation for achieving carbon neutrality and promoting environmentally-friendly composting practices.
作者机构：
[Yuan-Ping Deng; Rong Li; Xue-Ling Zhang; Xi-Long Yi; Guo-Hua Liu] Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
通讯机构：
[Guo-Hua Liu] R;Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
摘要：
Cattle ticks (Rhipicephalus microplus) are important economic ectoparasites causing direct and indirect damage to cattle and leading to severe economic losses in cattle husbandry. It is common knowledge that R. microplus is a species complex including five clades; however, the relationships within the R. microplus complex remain unresolved. In the present study, we assembled the complete mitochondrial genome of clade C by next-generation sequencing and proved its correctness based on long PCR amplification. It was 15,004 bp in length and consisted of 13 protein genes, 22 transfer genes, and two ribosomal genes located in the two strains. There were two copies of the repeat region (pseudo-nad1 and tRNA-Glu). Data revealed that cox1, cox2, and cox3 genes were conserved within R. microplus with small genetic differences. Ka/Ks ratios suggested that 12 protein genes (excluding nad6) may be neutral selection. The genetic and phylogenetic analyses indicated that clade C was greatly close to clade B. Findings in the current study provided more data for the identification and differentiation of the R. microplus complex and made up for the lack of information about R. microplus clade C.
摘要：
This study aimed to investigate the effects of supplementing laying hen diets with Radix Isatidis Polysaccharide (RIPS) on egg quality, immune function, and intestinal health. The research was conducted using 288 Hyland Brown hens, which were randomly assigned to four dietary treatments: control (without RIPS), low dose (200g/t), medium dose (500g/t), and high dose (1000g/t) of RIPS. Each dietary treatment was administered to eight replicates of nine hens for nine weeks. The results revealed that RIPS inclusion in diets significantly improved egg quality parameters such as egg shape index, yolk color, haugh unit, and protein height (P<0.05). Additionally, RIPS supplementation enhanced immune function as evidenced by an alteration in serum biochemical parameters, an increase in the spleen index, and a decrease in the liver index. Further, an evaluation of intestinal health showed that RIPS fortified the intestinal barrier, thus increasing the population of beneficial intestinal bacteria and reducing the abundance of harmful ones. Such mechanisms promoted intestinal health, digestion, and nutrient absorption, ultimately leading to enhanced egg quality. In conclusion, supplementing laying hen diets with RIPS has been demonstrated to improve egg quality by boosting immunity and optimizing intestinal digestion and absorption.
作者机构：
[Wang, Siqi; Li, Yuanyuan; Ning, Can; Fan, Hui; Xiao, Wenguang; Wu, You; Wang, Yongkang; Wu, Jing; Kong, Xiangyi; Yang, Chenglin; Wu, Aoao] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China;[Liang, Zengenni] Department of Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410128, PR China;[Sun, Zhiliang] Hunan Engineering Research Center of Veterinary Drugs, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China. Electronic address: zhiliangsun15@163.com;[Yuan, Zhihang] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China. Electronic address: zhyuan2016@hunau.edu.cn
通讯机构：
[Sun, Zhiliang; Yuan, Zhihang] H;Hunan Engineering Research Center of Veterinary Drugs, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China. Electronic address:;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China. Electronic address:
摘要：
Osteoarthritis (OA) is a degenerative joint disease, Increasingly, mitochondrial autophagy has been found to play an important regulatory role in the prevention and treatment of osteoarthritis. Koumine is a bioactive alkaloid extracted from the plant Gelsemium elegans. In previous research, Koumine was found to have potential in improving the progression of OA in rats. However, the specific mechanism of its action has not been fully explained. Therefore, the aim of this study was to investigate whether Koumine can alleviate OA in rats by influencing mitochondrial autophagy. In the in vitro study, rat chondrocytes (RCCS-1) were induced with IL-1β (10 ng/mL) to induce inflammation, and Koumine (50 μg/mL) was co-treated. In the in vivo study, a rat OA model was established by intra-articular injection of 2% papain, and Koumine was administered orally (1 mg/kg, once daily for two weeks). It was found that Koumine effectively reduced cartilage erosion in rats with osteoarthritis. Additionally, it decreased the levels of inflammatory factors such as IL-1β, IL-6, and extracellular matrix (ECM) components MMP13 and ADAMTS5 in chondrocytes and articular cartilage tissue, while increasing the level of Collagen II.Koumine inhibited the production of reactive oxygen species (ROS) in cartilage tissue and increased the number of autophagosomes in chondrocytes and articular cartilage tissue. Additionally, it upregulated the expression of mitochondrial autophagy proteins LC3Ⅱ/Ⅰ, PINK1, Parkin, and Drp1. The administration of Mdivi-1 (50 μM) reversed the enhanced effect of Koumine on mitochondrial autophagy, as well as its anti-inflammatory and anti-ECM degradation effects in rats with OA. These findings suggest that Koumine can alleviate chondrocyte inflammation and improve the progression of OA in rats by activating PINK1/Parkin-mediated mitochondrial autophagy.
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Viola yedoensis Makino (VYM) is a traditional Chinese herbal medicine widely distributed in China. It has many pharmacological effects such as anti-inflammatory, immune regulation and anti-oxidation. However, the protective effect of VYM on the spleen and thymus of broilers induced by heat stress has rarely been reported. AIM OF THE STUDY: We established a heat stress model of broilers to explore the protective effect of VYM on spleen and thymus of broilers. MATERIALS AND METHODS: In this experiment, a heat stress model was made by adjusting the feeding temperature of broilers. The protective effect of VYM on the spleen and thymus of heat-stressed broilers were evaluated by detecting immune organ coefficient, histological observation, Enzyme-Linked Immunosorbent Assay, production of antioxidant enzymes and peroxides, TUNEL Staining, Quantitative Real-time PCR. RESULTS: In this study, 60 healthy male AA broilers were divided into 6 groups: Control, 4.5% VYM, HS, HS + 0.5% VYM, HS + 1.5% VYM, HS + 4.5% VYM. After 42 days of feeding, serum, spleen and thymus were collected for detection and analysis. The study revealed that heat stress can lead to pathological damage in the spleen and thymus of broilers, reduce the content of immunoglobulin and newcastle disease (ND), infectious bursal disease (IBD) antibody levels, increase the expression of inflammatory factors IL-1β, INF-γ, heat shock 70kDa protein (HSP70), heat shock 90kDa protein (HSP90). Heat stress inhibits the activity of antioxidant enzymes CAT and SOD, promotes the production of MDA, and then lead to oxidative damage of the spleen and thymus. In addition, apoptotic cells and the ratio of Bax/Bcl-2 was increased. However, the addition of VYM to the feed can alleviate the adverse effects of heat stress on the spleen and thymus of broilers. CONCLUSIONS: This study showed that the addition of VYM to the diet could inhibit oxidative stress and apoptosis, and reduce the inflammatory damage of heat stress on the spleen and thymus of broilers. This study provides a basis for further exploring the regulatory role of VYM in heat stress-induced immune imbalance in broilers. In addition, this study also provides a theoretical basis for the development of VYM as a feed additive with immunomodulatory effects.
摘要：
Lilium is a commercially important genus of bulbous flowers, investigating the flowering molecular mechanisms is important for flowering regulation of lily. MADS-box SHORT VEGETATIVE PHASE (SVP) orthologs are involved in the flowering transition and floral organ differentiation in many plants. In this study, we identified an SVP ortholog from L. × formolongi (LfSVP), which was closely related to Arabidopsis SVP according to phylogenetic analysis. Tissue-specific expression patterns indicated that LfSVP expression levels peaked in the leaves and showed low expression levels in flowering tepals. Stage-dependent expression patterns of LfSVP showed high transcription level in the flowering induction stage under different photoperiods and exhibited transcription peak in the floral budding development stage under long days. Overexpressed LfSVP led to delayed flowering and floral organ defects in Arabidopsis independent of photoperiod. Tobacco rattle virus -induced gene silencing of LfSVP caused a strongly earlier flowering time and floral organ defects of L. × formolongi. Moreover, LfSVP can interact with L. × formolongi APETALA1 (AP1) in both yeast and tobacco cells, and the two may interact to regulate floral organ differentiation. In conclusion, LfSVP is a flowering repressor and may be involved in the regulation of floral organ differentiation. This study will be helpful for the molecular breeding of short-life-period and rich floral patterns lily varieties.
作者机构：
[Panpan Tong; Yueyi Dang; Juanjuan Pan; Ling Kuang] Xinjiang Key Laboratory of Herbivore Drug Research and Creation, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, China;College of Veterinary Medicine, Jilin University, Changchun, China;[Guangwei Song] Tecon Bio-Technology Co, LTD, Urumqi, China;[Lei Zhang] Aksu Regional Animal Disease Control and Diagnostic Center, Aksu, China;[Xiaozhen Song] People’s Government of Ziniquanzi Town, Ziniquanzi, China
通讯机构：
[Junhui Li] T;[Gang Lu] C;[Jinxin Xie] X;Tecon Bio-Technology Co, LTD, Urumqi, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha, China<&wdkj&>College of Veterinary Medicine, South China Agricultural University, Guangzhou, China<&wdkj&>Xinjiang Key Laboratory of Herbivore Drug Research and Creation, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, China<&wdkj&>Institute of Western Agriculture, The Chinese Academy of Agricultural Sciences, Changji, China
关键词：
Kirkovirus;Pigleta;Diarrheic disease
摘要：
Kirkovirus (kirV), a seemingly novel virus family, has been found in horses and donkeys. The study’s objectives are to investigate the presence of the virus in swine. In this study, donkey-like kirV was detected in rectal swabs of piglets with diarrhea, and the positive rate was found to be 100% (149/149). However, this virus was detected in only one of 261 clinically healthy piglets, which suggested a strong relationship between the kirV and the diarrheic disease. We obtained the whole-genome sequences of three kirVs (Cj-D5, Cj-D32, and Cj-D43), with a length of 3750 nucleotides (nt) and sharing 99.9% nt identity with donkey kirVs. Furthermore, the three viruses shared 88.5–100% and 23–51% of the Rep protein sequence, similar to available reference strains of Kirkoviridae and Circoviridae, respectively. Moreover, like horse and donkey kirVs, the RCR domain and P-loop NTPase domains of Rep protein and nonanucleotide motif (CAATATTAC) of the three viruses were similar to those of Circoviruses and Cycloviruses. Phylogenetic analysis showed that these viruses could be grouped with members in the proposed family Kirkoviridae. This is the first report to describe that kirV can circulate in piglets with diarrhea, and future studies are needed to determine the pathogenesis of this virus.
作者机构：
College of Horticulture, Shanxi Agricultural University, Taigu, China;[Zhen Wu, Zhen; Fu, Yongxia] Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan, China;College of Veterinary Medicine, Hunan Agricultural University, Changsha, China;[Qin, Nannan] Department of Development Planning and Cooperation, Shanxi Agricultural University, Taiyuan, China;[Wang, Haizhen; Zhao, Mengying] College of Horticulture, Shanxi Agricultural University, Taigu, China<&wdkj&>Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan, China
通讯机构：
[Yongxia Fu] S;[Shang Guo] C;Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan, China<&wdkj&>College of Horticulture, Shanxi Agricultural University, Taigu, China<&wdkj&>Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
摘要：
Daylily is a functional food with high nutritional value in China. Datong (DT) in Shanxi Province is one of the four main production areas of daylily. Therefore, Linfen (LF), Lvliang (LL), and Yangquan (YQ) in Shanxi Province have also introduced daylily from DT. However, geographical and climatic conditions and producing patterns cause variations in the daylily quality. In the present study, we found that the nutrient composition of daylilies from different producing areas of Shanxi Province varied. The key environmental factors affecting the nutrition of daylily in different regions were altitude and temperature. The widely targeted metabolomics results showed that 1642 metabolites were found in daylily. The differential metabolites between DT and YQ, LL and LF were 557, 667, and 359, respectively. Notably, 9 metabolic pathways and 59 metabolite markers were associated with daylily from different areas. This study provides a theoretical basis for the quality maintenance and health efficacy research of daylily.
摘要：
INTRODUCTION: Excessive intake of lactate caused by improper use of silage in animal husbandry has adverse effects on rumen fermentation, such as rumen acidosis. The speed of absorption and metabolism of D-lactate in rumen epithelial cells was slower than that of L-lactate, making D-lactate more prone to accumulate and induce rumen acidosis. Therefore, this study was conducted to explore the effects of dietary D-lactate levels on rumen fermentation of beef cattle and its mechanism in an in vitro system. METHODS: This experiment was adopted in single-factor random trial design, with 5 days for adaptation and 3 days for sample collection. Three treatments (n = 8/treatment) were used: (1) D-LA (0.3%), basal fermentation substrate with 0.3% (dry matter, DM basis) D-lactate; (2) D-LA (0.75%), basal fermentation substrate with 0.75% (DM basis) D-lactate; and (3) D-LA (1.2%), basal fermentation substrate with 1.2% (DM basis) D-lactate. RESULTS: With the dietary D-lactate levels increased, the daily production of total gas, hydrogen and methane, as well as the ruminal concentrations of acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, total volatile fatty acid and D-lactate increased (p < 0.05), but the ruminal pH and acetate/propionate ratios decreased (p < 0.05). Principle coordinate analysis based on Bray-Curtis distance showed that increasing dietary D-lactate levels could significantly affect the structure of rumen bacterial community (p < 0.05), but had no significant effect on the structure of rumen eukaryotic community (p > 0.05). NK4A214_group, Ruminococcus_gauvreauii_group, Eubacterium_oxidoreducens_group, Escherichia-Shigella, Marvinbryantia and Entodinium were enriched in D-LA (1.2%) group (p < 0.05), as well as WCHB1-41, vadinBE97, Clostridium_sensu_stricto_1, Anaeroplasma and Ruminococcus were enriched in D-LA (0.3%) group (p < 0.05). Changes in the composition of ruminal microorganisms affected rumen metabolism, mainly focus on the biosynthesis of glycosaminoglycans (p < 0.05). DISCUSSION: Overall, feeding whole-plant corn silage with high D-lactate content could not induce rumen acidosis, and the metabolization of dietary D-lactate into volatile fatty acids increased the energy supply of beef cattle. However, it also increased the ruminal CH(4) emissions and the relative abundance of opportunistic pathogen Escherichia-Shigella in beef cattle. The relative abundance of Verrucomicrobiota and Escherichia-Shigella may be influenced by glycosaminoglycans, reflecting the interaction between rumen microorganisms and metabolites.
期刊：
Brazilian Journal of Poultry Science,2024年26(1):1-16 ISSN：1516-635X
通讯作者：
Guo, S
作者机构：
[Zi, Q.; Guo, S.; Li, P.; Qu, X.; Lei, J.; He, C.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Zi, Q.; Guo, S.; Li, P.; Qu, X.; Lei, J.; He, C.] Hunan Agr Univ, Hunan Engn Res Ctr Poultry Prod Safety, Changsha 410128, Hunan, Peoples R China.;[Yao, Y.] Huaihua Ctr Anim Husb & Fisheries Affairs, Huaihua 418000, Hunan, Peoples R China.;[Yao, Y.] Hunan Yunfeifeng Agr Co Ltd, Res & Dev Ctr, Huaihua 418200, Hunan, Peoples R China.;[Zou, X.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Guo, S ] N;Nongda Rd 1, Changsha 410000, Hunan, Peoples R China.
关键词：
Blue eggshell;DNA methylation;eggshell glands;transcriptome;Xuefeng Black-bone chicken
摘要：
Blue eggshells have become economically valuable due to their higher popularity among consumers when compared with eggs of other colors, particularly in East Asia. However, eggshell colors vary widely and gradually become lighter with the increase of age. Therefore, to determine the association between DNA methylation and gene expression on the molecular mechanism of eggshell color variation in Xuefeng Black-bone chickens, we collected the shell glands from chickens that produced dark blue eggshells and light blue eggshells, respectively, and performed whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-Seq) on them. The results showed that in the context of CG, DNA methylation levels were the highest compared to CHG and CHH contexts, and were negatively correlated with gene expression levels in three different gene regions in all samples, while there was no significant correlation in the contexts of CHG and CHH. Furthermore, when disregarding the location of coding genes, a total of 55 genes will show differences not only in expression levels but also in DNA methylation levels, and among the majority of differential expression genes (n=50), the DNA methylation levels in the gene body region were the highest. GO analysis and KEGG pathway analysis were performed in these genes and the results showed that the genes NR1H4 and HEPHL1, ABCA4 and ABCA12, and the pathways, cell adhesion molecular, ABC transporters, ECM-receptor interaction, and neuroactive ligand-receptor interaction, may affect the color variation of the blue eggshell of Xuefeng Black-bone chickens.
作者机构：
[Wang, Yongsheng] College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;These authors contributed equally to this work.;[Pan, Chuanying; Bi, Yi; Yang, Yuanzhe; Li, Jie] College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;Authors to whom correspondence should be addressed.;College of Veterinary Medicine, Hunan Agricultural University, Changsha 410125, China
通讯机构：
[Shuilian Wang; Xianyong Lan] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410125, China
摘要：
The high-mobility group AT-hook 2(HMGA2) gene has been widely studied in the context of cancer and animal growth. However, recently, several studies have uncovered its critical role in cell proliferation. A genome-wide association study (GWAS) further suggests that the HMGA2 gene is a candidate gene in fertility, indicating its connection not only to growth traits but also to reproduction, specifically ovarian traits. Thus, this study aimed to analyze the distribution of the HMGA2 gene in 54 bovine breeds worldwide, identify important short fragment variants (indels), and investigate the relationship between HMGA2 and ovarian development. The dataset included genotypic information from a bovine population of 634 individuals (n = 634). After genotyping and analyzing four selected loci, we found that one out of four loci, rs133750033 (P4-D(22-bp)), was polymorphic. Our results also reveal that this indel of HMGA2 is significantly associated with certain ovarian traits (p < 0.05). Specifically, it has connection with ovarian length (p = 0.004) and ovarian height (p = 0.026) during diestrus. Additionally, we discovered a higher expression of the HMGA2 gene in Asian cattle breeds. In summary, this study suggests that HMGA2 has the potential to serve as an animal fertility testing marker gene. Moreover, these findings contribute to a more promising outlook for the bovine industry.
关键词：
TCM polysaccharides;salpingitis;immunity and antioxidants;gut microbiota;serum metabolome
摘要：
This paper aimed to evaluate the effect of 3 kinds of TCM polysaccharides instead of antibiotics in preventing salpingitis in laying hens. After feeding the laying hens with Lotus leaf polysaccharide, Poria polysaccharide, and Epimedium polysaccharide, mixed bacteria (E. coli and Staphylococcus aureus) were used to infect the oviduct to establish an inflammation model. Changes in antioxidant, serum immunity, anti-inflammatory, gut microbiota, and serum metabolites were evaluated. The results showed that the 3 TCM polysaccharides could increase the expression of antioxidant markers SOD, GSH, and CAT, and reduce the accumulation of MDA in the liver; the contents of IgA and IgM in serum were increased. Decreased the mRNA expression of TLR4, NFκB, TNF-α, IFN-γ, IL1β, IL6, and IL8, and increased the mRNA expression of anti-inflammatory factor IL5 in oviduct tissue. 16sRNA high-throughput sequencing revealed that the 3 TCM polysaccharides improved the intestinal flora disturbance caused by bacterial infection, increased the abundance of beneficial bacteria such as Bacteroides and Actinobacillus, and decreased the abundance of harmful bacteria such as Romboutsia, Turicibacter, and Streptococcus. Metabolomics showed that the 3 TCM polysaccharides could increase the content of metabolites such as 3-hydroxybutyric acid and isobutyl-L-carnitine, and these results could alleviate the further development of salpingitis. In conclusion, the present study has found that using TCM polysaccharides instead of antibiotics was a feasible way to prevent bacterial salpingitis in laying hens, which might make preventing this disease no longer an issue for breeding laying hens.
摘要：
BACKGROUND: Buffalo milk, constituting 15% of global production, has higher fatty acids content than Holstein milk.Fourier transform mid-infrared spectroscopy (FT-MIR) is widely used for dairy analysis, but its application to buffalo milk, with larger fat globules, remains understudied. The ultimate goal of this study is to develop machine learning models based on FT-MIR for predicting fatty acids in buffalo milk and to assess the accuracy of commercial milk analyzers. This research provides a convenient, fast, and environmentally friendly method for detecting the fatty acid composition in buffalo milk. RESULTS: We employed six machine learning algorithms to establish a detection model for 34 fatty acids in buffalo milk. The predictive models demonstrated robust capabilities for high-content fatty acids (C14:0, C15:0, C16:0, C17:0, C18:0, C18:1, SFA, MUFA), with errors within a 15% range. Traditional FT6000 detection methods exhibited limitations in measuring saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA). Implementing a mean difference correction of 0.21 for monounsaturated fatty acids and applying regression equations (SFA×1.0639+0.0705; PUFA×0.5472+0.0047) significantly improved measurement accuracy. CONCLUSION: This study successfully developed a predictive model for fatty acids in Mediterranean buffalo milk based on FT-MIR. Additionally, a correction was applied to the existing measurement device, FT-6000, enabling more accurate measurements of fatty acids in buffalo milk. The findings have practical implications for the food industry, offering a faster and more reliable approach to assess and monitor fatty acid composition in buffalo milk, potentially influencing product development and quality control processes. This article is protected by copyright. All rights reserved.
摘要：
Microplastics (MPs) are global environmental pollutants with potential toxicity concerns, and their effects on the reproductive system have attracted increasing attention. This study investigated the interaction between MPs and mammalian biomolecules, focusing on the relationship between the testosterone adsorption behavior of MPs and male reproductive health. The adsorption capacity of different types of MPs for testosterone was evaluated in vitro experiments. Polyamide (PA)-MPs exhibited stronger adsorption, while polymethyl methacrylate (PMMA)-MPs displayed the weakest adsorption. Sorption equilibrium between PA-MPs and testosterone was achieved within 6h, fitting the Pseudo-2nd-order model and Langmuir isotherm. The effects of MPs on male reproduction in mice was determined in vivo experiments. Male mice were treated with 0.1 and 0.5mg/d PA-MPs/PMMA-MPs by gavage once per day for 28 days. The results showed that only 0.5mg/d PA-MP exposure induced decreased serum testosterone levels, increased testicular testosterone levels compared to the control, and more severe damage to seminiferous tubule structure, sperm motility and sperm morphology compared to the PMMA-MPs group. Meanwhile, PA-MPs could reduce intracellular nuclear translocation of androgen receptor (AR) mediated by testosterone, while PMMA-MPs had no impact. The study revealed that PA-MP adsorption reduced testosterone bioavailability and caused sperm quality to decline, offering new insights into the combined toxicity mechanism of MPs in male mammals.
期刊：
Frontiers in Veterinary Science,2023年10:1314624 ISSN：2297-1769
通讯作者：
Wang, AB;Yao, J
作者机构：
[Tan, Lei] Yangtze Univ, Coll Anim Sci, Jingzhou, Peoples R China.;[Wang, Aibing; Wang, AB; Tan, Lei; Wang, Kaixin] Hunan Agr Univ, Coll Vet Med, Changsha, Peoples R China.;[Zhang, Shuo; Bai, Ping] Yunnan Southwest Agr & Anim Husb Grp Co Ltd, Kunming, Peoples R China.;[Zuo, Mengting] Changsha Med Univ, Hunan Prov Key Lab TCM Agr Biogenom, Changsha, Hunan, Peoples R China.;[Shu, Xianghua] Yunnan Agr Univ, Coll Anim Med, Kunming, Peoples R China.
通讯机构：
[Yao, J ] Y;[Wang, AB ] H;Hunan Agr Univ, Coll Vet Med, Changsha, Peoples R China.;Yunnan Anim Sci & Vet Inst, Yunnan Trop & Subtrop Anim Virus Dis Lab, Kunming, Peoples R China.
关键词：
antiviral strategies;cellular factors;involvement;pseudorabies virus;viral attachment and entry
摘要：
Pseudorabies virus (PRV) belongs to the Alphaherpesvirinae subfamily and serves as an exceptional animal model for investigating the infection mechanism of Herpes simplex virus type 1. Notably, PRV has the capability to infect a wide range of mammals, including humans, highlighting its potential as an overlooked zoonotic pathogen. The attachment and entry steps of PRV into host cells are crucial to accomplish its life cycle, which involve numerous cellular factors. In this mini review, we offer a comprehensive summary of current researches pertaining to the role of cellular factors in PRV attachment and entry stages, with the overarching goal of advancing the development of novel antiviral agents against this pathogen.
