基于香气指纹图谱和多元化学计量法对黄金茶2号等级的判别分析
作者:
巢瑾;周令欣;银飞燕;罗茜;赵萌萌;...
期刊:
食品科学 ,2023年44(4):321-328 ISSN:1002-6630
作者机构:
湖南农业大学食品科学技术学院,,国家茶叶加工技术研发分中心,,湖南省茶业集团股份有限公司;国家茶叶加工技术研发分中心,,湖南省茶业集团股份有限公司;[蒋立文; 李跑] 湖南农业大学食品科学技术学院;[周令欣; 赵萌萌; 袁勇; 罗茜; 吴浩人; 银飞燕] 湖南省茶叶种植与加工工程技术研发中心<&wdkj&>湖南省茶业集团股份有限公司;[巢瑾] 湖南农业大学食品科学技术学院<&wdkj&>湖南省茶叶种植与加工工程技术研发中心<&wdkj&>湖南省茶业集团股份有限公司
关键词:
黄金茶;顶空固相微萃取;香气指纹图谱;等级判别;化学计量学
摘要:
本文以黄金茶2号为研究对象,采用顶空固相微萃取与气相色谱-质谱联用技术(headspace solid-phase micro extraction/gas chromatography-mass spectrometry, HS-SPME/GC-MS),建立不同等级黄金茶香气成分GC-MS指纹图谱,并通过化学计量法对两个等级的黄金茶进行判别分析。研究表明,2个等级的黄金茶中鉴定出81种香气组分,其中41种为共有香气组分。基于共有香气组分的正交偏最小二乘方判别分析(orthogonal partial least squares discrimination analysis, OPLS-DA)可以区分2个等级黄金茶,并筛选出9种差异香气组分,分别是庚醛、柠檬烯、反式-β-罗勒烯、1-辛醇、反式-4,8-二甲基-1,3,7-壬三烯、反-橙花叔醇、1,10-Diepicubenol、Epicubenol、十六酸甲酯。以9种差异香气组分为变量建立的线性判别方程对2个等级的黄金茶进行等级判别,自身验证和交叉验证正确率为100%。本文为黄金茶的分级提供了客观量化的参考依据。
语种:
中文
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菜籽油加工技术研究进展
作者:
蒋璐;廖卢艳;吴卫国
期刊:
中国粮油学报 ,2023年38(3):179-187 ISSN:1003-0174
作者机构:
[吴卫国; 蒋璐; 廖卢艳] 湖南农业大学食品科学技术学院
关键词:
菜籽油;油料预处理技术;精炼
摘要:
菜籽油是我国主要食用油之一,富含油酸、亚油酸、维生素E、植物甾醇、胡萝卜素和菜籽多酚等功能性物质,营养价值高,因其独特的风味备受欢迎,但不同的加工技术对菜籽油品质具有较大影响。本文综述了国内外常用的菜籽油加工技术研究进展,着重阐述了加工技术对菜籽油品质的影响机制,旨在为进一步优化菜籽油加工技术,提高油品质量和促进菜籽油的适度加工提供一定的参考。
语种:
中文
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Pine pollen: A review of its chemical composition, health effects, processing, and food applications
作者:
Cheng, Yong;Wang, Zhenyu;Quan, Wei;Xue, Chaoyi;Qu, Tao;...
期刊:
Trends in Food Science & Technology ,2023年138:599-614 ISSN:0924-2244
通讯作者:
Chen, Jie;He, ZY
作者机构:
[Chen, Qiuming; He, Zhiyong; Cheng, Yong; Zeng, Maomao; Chen, Jie; Wang, Zhaojun; Qin, Fang; Xue, Chaoyi; Wang, Zhenyu] Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Jiangsu, Peoples R China.;[Chen, Qiuming; He, Zhiyong; Cheng, Yong; Zeng, Maomao; Chen, Jie; Wang, Zhaojun; Qin, Fang; Xue, Chaoyi; Wang, Zhenyu] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Jiangsu, Peoples R China.;[Quan, Wei] Hunan Agr Univ, Sch Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Wang, Tong; Qu, Tao] Yantai New Era Hlth Ind Co Ltd, Yantai 264000, Shandong, Peoples R China.;[He, ZY; Chen, J] Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Jiangsu, Peoples R China.
通讯机构:
[Chen, J; He, ZY ] ;Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Jiangsu, Peoples R China.
关键词:
Pine pollen;Nutraceutical;Health benefit;Processing;Storage;Food application
摘要:
Background:Pine pollen, well-known as a "natural micro-nutrient bank," is the male spore of Pinus massoniana Lamb., Pinus tabuliformis Carr., and other plants of the same genus. Pine pollen has a long history of use in China as a traditional medicinal and edible material due to its rich nutrients, bioactive compounds, and health potential. Scope and approach:This review summarizes the published findings related to chemical compositions, health effects, processing and storage, and food applications of pine pollen. The possible mechanisms behind the beneficial properties of pine pollen, and the future direction for research and development are also underlined. Key findings and conclusions:Pine pollen is a rich source of macronutrients and bioactive compounds that have demonstrated various health benefits, including antioxidant, immunomodulatory, anti-inflammatory, glucose and lipid metabolism regulatory, antimicrobial and antiviral, antitumor and anticancer, hepatoprotective, gastrointestinal modulatory, anti-aging, and other biological properties. The nutritional quality and beneficial effects of pine pollen are influenced by post-harvest processing methods. Pine pollen has diverse applications in the food industry, such as in beverages, pastries, dairy products, delivery systems, and as sorbents for separation and purification of polar analytes. Further in vivo experiments and clinical trials are needed to deepen our understanding of its health benefits. Exploring combinations of multiple processing methods could optimize the utilization of pine pollen as a nutritional food resource.
语种:
英文
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植物源性食品中多农药残留GC-MS高通量快速检测技术研究进展
作者:
雷紫依;苏光林;李跑;刘洋
期刊:
分析测试学报 ,2023年42(10):1370-1380 ISSN:1004-4957
作者机构:
[苏光林; 刘洋; 雷紫依] 湖南农业大学食品科学技术学院食品科学与生物技术湖南省重点实验室;湖南省农业科学院湖南省农产品加工研究所;[李跑] 湖南农业大学食品科学技术学院食品科学与生物技术湖南省重点实验室<&wdkj&>湖南省农业科学院湖南省农产品加工研究所
关键词:
气相色谱-质谱联用(GC-MS);多农药残留;高通量检测;样品前处理;化学计量学
摘要:
植物源性食品基质复杂、农药种类繁多、农药残留限量标准严格,因此建立植物源性食品中多农药残留高通量快速检测技术十分重要。气相色谱-质谱联用(GC-MS)技术是多农药残留高通量检测领域应用最为广泛的一种技术。然而现阶段植物源性食品中多农药残留GC-MS高通量检测仍存在问题。国内外科研工作者对其进行了大量研究,在现有设备的基础上开发前处理新技术以及复杂信号高通量解析新算法是提高该检测水平的关键。该文对近年来国内外发展的基于GC-MS的植物源性食品中多农药残留高通量快速检测技术及相关文献进行了归纳和总结,重点从适合于不同种类农药提取的新型样品前处理技术、基于“数学分离”的复杂信号高通量解析新算法等方面阐述了植物源性食品中多农药残留高通量快速检测的最新进展,并对其存在的问题提出了相关建议,旨在为食品多农药残留高通量快速检测提供参考。
语种:
中文
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A novel strategy to construct stable fat globules with all major milk fat globule membrane proteins to mimic breast milk fat emulsions at the protein level
作者:
Wang, Yi;Guo, Mengyuan;Ren, Fazheng;Wang, Pengjie;Li, Hongjuan;...
期刊:
Food Research International ,2023年173(Pt 1):113351 ISSN:0963-9969
通讯作者:
Yu, JH;Li, YX;Luo, J
作者机构:
[Wang, Yi; Li, Hongbo; Yu, Jinghua; Li, Hongjuan; Yu, JH] Tianjin Univ Sci & Technol, Coll Food Sci & Engn, Tianjin 300457, Peoples R China.;[Wang, Yi; Li, Yixuan; Li, YX; Guo, Mengyuan; Wang, Pengjie; Ren, Fazheng] China Agr Univ, Dept Nutr & Hlth, Beijing 100083, Peoples R China.;[Guo, Mengyuan; Ren, Fazheng] China Agr Univ, Coll Food Sci & Nutr Engn, Key Lab Funct Dairy Coconstructed, Minist Educ & Beijing Govt, Beijing 100083, Peoples R China.;[Luo, Jie] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410114, Peoples R China.
通讯机构:
[Luo, J ] H;[Li, YX ] C;[Yu, JH ] T;Tianjin Univ Sci & Technol, Coll Food Sci & Engn, Tianjin 300457, Peoples R China.;China Agr Univ, Dept Nutr & Hlth, Beijing 100083, Peoples R China.
关键词:
Cream;Emulsion;Label-free proteomic approach;MFGM proteins;Oil/water interface
摘要:
Milk fat globule membrane (MFGM) proteins have several biological functions and maintain the fat globule structure. However, the major MFGM protein compositions in simulated human milk emulsions are different from those in human milk due to the composition loss in the isolation process of MFGM materials. To overcome this limitation, we developed a novel strategy, namely, the solution enriched with MFGM was homogenized with cream separated from the milk rich in large-sized fat globules. The results of physicochemical properties and the interfacial protein coverage of the emulsions showed that the emulsions prepared by the new method had a smaller particle size, higher stability, and more interfacial protein coverage when the ratio of fat to protein was 1:3. In addition, proteome differences in interfacial proteins between the new emulsions and simulated infant formula emulsions were investigated, and the results revealed that the interface of the emulsions prepared by the new method contained all major MFGM proteins and unique GO annotations and KEGG pathways. However, only four MFGM proteins (XO, ADPH, PAS 6/7) were quantified at the interface of the emulsions prepared by the common method. Furthermore, the protein number and the total relative abundance of major MFGM proteins were approximately 2-fold and 475-fold higher at the interface of the emulsions prepared by the new method compared to the common method. Overall, the study modulated the interfacial protein composition of fat globules by screening the sources of lipid and homogenization methods and revealed its potential effect on processing stability and biological properties.
语种:
英文
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基于氨丙基SiO2微球固相萃取-高效液相色谱法测定花生中白藜芦醇
作者:
周千;喻理;马飞;范伟;覃静萍;...
期刊:
食品安全质量检测学报 ,2023年14(06):160-166 ISSN:2095-0381
作者机构:
[周千; 郭时印; 范伟; 唐忠海] 湖南农业大学食品科学技术学院;[喻理; 马飞] 中国农业科学院油料作物研究所农业农村部油料作物生物学与遗传育种重点实验室农业农村部油料及制品质量监督检验测试中心农业农村部油料产品质量安全风险评估实验室(武汉);[覃静萍] 湖南农业大学生物科学技术学院;[肖航] 麻省大学阿莫斯特分校食品科学系;湖北省洪山实验室
关键词:
花生;白藜芦醇;固相萃取;高效液相色谱法
摘要:
目的 建立一种固相萃取-高效液相色谱法测定花生中白藜芦醇含量的分析方法.方法 样品用 85%乙醇提取,用氨丙基SiO2 微球材料填充固相萃取净化小柱富集,乙醇作为淋洗溶液,吹干后用 0.2 mL甲醇定容检测.采用 C18 色谱柱,以乙腈和水为流动相等度洗脱分离,检测波长为 306 nm 检测.结果 该方法在0.1~10.0 μg/g的范围内,白藜芦醇线性关系良好(r2>0.999),检出限为0.017 μg/g,定量限为0.056 μg/g;加标回收率为 90.4%~107.2%,相对标准偏差为 1.3%~8.4%.运用该方法对 30 份花生样品检测,其白藜芦醇含量范围在 73.24~548.51 μg/kg之间.结论 该方法灵敏度高、重复性好、成本低廉,且操作简单、快速,满足花生中白藜芦醇定量分析的要求.
语种:
中文
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Acteoside palliates D-galactose induced cognitive impairment by regulating intestinal homeostasis
作者:
Li, Maiquan;Zhu, Mingzhi;Quan, Wei;Huang, Weisu;Liu, Xia;...
期刊:
Food Chemistry ,2023年421:135978 ISSN:0308-8146
通讯作者:
Liu, ZH;Xiao, X
作者机构:
[Quan, Wei; Li, Maiquan; Zhang, Can; Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Peoples R China.;[Liu, Zhonghua; Li, Maiquan; Zhu, Mingzhi] Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Key Lab Tea Sci Minist Educ, Changsha 410128, Peoples R China.;[Xiao, X; Xiao, Xing] Hunan Prov Peoples Hosp, Changsha 410128, Peoples R China.;[Huang, Weisu] Zhejiang Econ & Trade Polytech, Dept Appl Technol, Hangzhou 310018, Peoples R China.;[Lu, Baiyi] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China.
通讯机构:
[Xiao, X ; Liu, ZH ] H;Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Key Lab Tea Sci Minist Educ, Changsha 410128, Peoples R China.;Hunan Prov Peoples Hosp, Changsha 410128, Peoples R China.
关键词:
Acteoside;Antiaging;Cognitive impairment;Intestinal homeostasis
摘要:
Acteoside, an important phenylethanol glycoside, is the main active component in Osmanthus fragrans flower. Our previous study found that acteoside showed high antiaging effect but its absorption rate was low. We speculated acteoside palliated aging-related cognitive impairment before being absorbed, that was intestinal homeostasis underlie the antiaging effect of acteoside. In this study, acteoside was confirmed to palliate cognitive impairment in D-galactose induced aging mice. Acteoside treatment dramatically reduced oxidative stress, alleviated intestinal inflammation, restored intestinal mucosal barrier, rebuilt gut microbiome structure and upregulated gut microbiome metabolites short-chain fatty acids (SCFAs) and amino acids (AAs). Furthermore, antibiotic treatment revealed that the antiaging ability of acteoside was abolished in microbiota depleted mice, which offered direct evidence for the essential role of gut microbiota in the attenuation of cognitive impairment of acteoside. Together, our study indicated that acteoside palliated cognitive impairment by regulating intestinal homeostasisand acteoside intake might be a promising nutritional intervention in prevention of neurodegenerative diseases. © 2023
语种:
英文
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分散泛菌胞内脱乙酰酶在大肠杆菌中重组表达条件优化及酶学特性
作者:
杨文韬;庞立;田红;陈晓;易有金;...
期刊:
食品安全质量检测学报 ,2023年14(22):190-199 ISSN:2095-0381
作者机构:
湖南农业大学食品科学技术学院, 长沙 410000;湖南农业大学园艺园林学院, 长沙 410000;[夏菠; 杨文韬; 易有金; 田红; 陈晓; 庞立] 湖南农业大学
关键词:
分散泛菌;N-乙酰氨基葡萄糖脱乙酰酶;重组表达;酶学特性
摘要:
目的 对分散泛菌的胞内脱乙酰酶(Pantoea dispersa N-acetylglucosamine deacetylase,Pd-nagA)进行表达条件优化及酶学性质分析.方法 本研究使用聚合酶链式反应(polymerase chain reaction,PCR)技术对分散泛菌全基因组中Pd-nagA基因序列进行全长扩增,将含有Pd-nagA基因的质粒pET-28a(+)转化进入大肠杆菌BL21(DE3)中诱导表达,同时采用单因素实验,优化诱导温度、诱导前菌体生物量、诱导时间以及乳糖类似物异丙基硫代半乳糖苷(isopropyl β-D-1-thiogalactopyranoside,IPTG)的浓度,采用 Ni2+-次氮基三乙酸(nitrilotriacetic acid,NTA)进行重组酶的纯化,再对其酶学特性进行测定.结果 以pET-28a(+)质粒作为表达载体,大肠杆菌BL21(DE3)成功表达了可溶形式的Pd-nagA蛋白,分子量约为 40 kDa.当诱导温度为 28℃,诱导前菌液OD600 值为 1.2,IPTG浓度为 0.6 mmol/L,诱导时间为 20 h时,Pd-nagA蛋白得到了大量表达.通过薄层层析法分析表明Pd-nagA重组蛋白能够在体外转化N-乙酰氨基葡萄糖生成氨基葡萄糖,Pd-nagA重组蛋白能够在高盐溶液中保持一半的相对酶活性,该酶 4℃储存 10 d的相对活性仍然保持在 90%以上,具有良好的储存稳定性,催化N-乙酰氨基葡萄糖生成氨基葡萄糖反应条件简单快捷.结论 本研究成功异源表达了具有催化活性的 Pd-nagA 酶,并对表达条件进行优化及酶学特性探究,可为工业上单酶催化制备氨基葡萄糖提供一定的理论参考.
语种:
中文
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Vitamin B3 inhibits apoptosis and promotes autophagy of islet β cells under high glucose stress
作者:
Zhang, Y. U.;Zhou, Xian;Zhang, Chunyan;Lai, Dengni;Liu, Dengbo;...
期刊:
生物细胞(英文) ,2023年47(4):859-868 ISSN:0327-9545
通讯作者:
Wu, Y.
作者机构:
[Liu, Dengbo; Zhang, Chunyan; Zhou, Xian; Wu, Yanyang; Zhang, Y. U.] Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Changsha 410128, Peoples R China.;[Zhang, Chunyan; Zhou, Xian; Wu, Yanyang; Zhang, Y. U.] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Peoples R China.;[Liu, Dengbo; Wu, Yanyang] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Peoples R China.;[Liu, Dengbo; Wu, Yanyang] State Key Lab Subhlth Intervent Technol, Changsha 410128, Peoples R China.;[Lai, Dengni] Hunan Agr Prod Proc Inst, Hunan Food Test & Anal Ctr, Changsha 410004, Peoples R China.
通讯机构:
[Wu, Y.] K;Key Laboratory for Food Science and Biotechnology of Hunan Province, China
关键词:
Abbreviations;Vitamin B3;High glucose;Autophagy;Apoptosis
摘要:
Background: Hyperglycemia is a typical symptom of diabetes. High glucose induces apoptosis of islet β cells. While autophagy functions in cytoprotection and autophagic cell death. The interaction between autophagy and apoptosis is important in the modulation of the function of islet β cells. Vitamin B3 can induce autophagy and inhibit islet β apoptosis. Method: The mechanism of vitamin B3-mediated protective effect on the function of islet β cells was explored by the method of western blot, immunofluorescence and flow cytometry. Results: In the present study, high glucose stress increased the apoptosis rate, while vitamin B3 reduced the apoptosis rate. The effect of vitamin B3 on autophagy flux under normal and high glucose stress was also investigated. Vitamin B3 increased the number of autophagosomes and increased the light chain (LC)3-II/LC3-I ratio. In contrast, vitamin B3 decreased sequestosome 1 (SQSTM1)/p62 protein expression and inhibited the phosphorylation of mammalian ribosomal protein S6 kinase β-1 (p70S6K/S6K1), which was a substrate of mammalian target of rapamycin (mTOR) under normal and high glucose stress. To further verify the protective effect of vitamin B3 on apoptosis, we treated islet β cell RIN-m5F with autophagy inhibitor 3-methyladenine (3-MA). Vitamin B3 decreased the apoptosis rate under high glucose stress, while the inhibition of apoptosis by vitamin B3 was blocked after adding 3-MA. Conclusion: Our data suggested that vitamin B3 reduced the apoptosis rate of β cells, possibly through inducing autophagy under high glucose stress. © 2023 Centro Regional de Invest. Cientif. y Tecn.. All rights reserved.
语种:
英文
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Extraction of Ursolic Acid from Apple Peel with Hydrophobic Deep Eutectic Solvents: Comparison between Response Surface Methodology and Artificial Neural Networks
作者:
Li, Haiyan;Liu, Yugang;Guo, Shiyin;Shi, Meng;Qin, Si;...
期刊:
Foods ,2023年12(2):310- ISSN:2304-8158
通讯作者:
Chaoxi Zeng
作者机构:
[Zeng, Chaoxi; Li, Haiyan; Qin, Si; Shi, Meng; Liu, Yugang; Guo, Shiyin] Hunan Agr Univ, Coll Food Sci & Technol, Dept Biol & Med, 1 Nongda Rd, Changsha 410128, Peoples R China.
通讯机构:
[Chaoxi Zeng] A;Author to whom correspondence should be addressed.<&wdkj&>Department of Biology and Medicine, College of Food Science and Technology, Hunan Agricultural University, No. 1 Nongda Road, Furong District, Changsha 410128, China
关键词:
apple peel;ultrasonic-assisted extraction;response surface methodology;artificial neural network
摘要:
Extracting ursolic acid (UA) from plant resources using organic solvents is incompatible with food applications. To address this, in this study, 15 edible hydrophobic deep eutectic solvents (HDESs) were prepared to extract UA from apple peel, the extraction conditions were optimized, and the optimization strategies were compared. It was found that the solubility of UA in the HDESs can be 9 times higher than the traditional solvent such as ethanol. The response surface optimization concluded that temperature had the greatest effect on the extraction and the optimized test conditions obtained as follows: temperature of 49 °C, time of 32 min, solid–liquid ratio of 1:16.5 g/mL, respectively. Comparing the response surface methodology (RSM) and artificial neural networks (ANN), it was concluded that ANN has more accurate prediction ability than RSM. Overall, the HDESs are more effective and environmentally friendly than conventional organic solvents to extract UA. The results of this study will facilitate the further exploration of HDES in various food and pharmaceutical applications. © 2023 by the authors.
语种:
英文
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Phytochemical activators of Nrf2: a review of therapeutic strategies in diabetes
作者:
Qin, Si;Chen, Zhong;Wen, Yiling;Yi, Yuhang;Lv, Chenghao;...
期刊:
生物化学与生物物理学报 ,2023年55(1):11-22 ISSN:1672-9145
通讯作者:
Shi, M.;Chen, L.;Qin, S.
作者机构:
[Zeng, Chaoxi; Wen, Yiling; Yi, Yuhang; Qin, Si; Chen, Zhong; Shi, Meng] Hunan Agr Univ, Coll Food Sci & Technol, Lab Food Funct & Nutrigen, Changsha 410128, Peoples R China.;[Qin, Si] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Peoples R China.;[Chen, Lanming] Shanghai Ocean Univ, Coll Food Sci & Technol, Key Lab Qual & Safety Risk Assessment Aquat Prod, Minist Agr & Rural Affairs Peoples Republ China, Shanghai 201306, Peoples R China.
通讯机构:
[Qin, S.; Shi, M.] L;[Chen, L.] K;Lab of Food Function and Nutrigenomics, China;Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China
关键词:
phytochemical;Nrf2-ARE;insulin resistance;diabetes;chemoprevention;gut microbiota
摘要:
Insulin resistance (IR) is fundamental to the development of type 2 diabetes (T2D), and altered mitochondrial function and abnormal lipid distribution are closely associated with IR or T2D. Excess oxidative stress-induced mitochondrial damage leads to an imbalance in redox homeostasis, which is considered the major contributor to the progression of diabetes. A key cellular defense mechanism, namely, the nuclear factor-E2 p45-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway, plays an essential protective role in combating excess oxidative stress. A series of phytochemicals are reported to improve IR and restore mitochondrial function against excess oxidative stress by activating the Nrf2-ARE signaling pathway to maintain cellular reactive oxygen species (ROS) homeostasis. The present review focuses on key knowledge gaps in the Nrf2-ARE system targeted by phytochemicals and its correlation to diabetes both in the in vitro and in vivo models and recent achievements in human clinical trials to evaluate its efficiency and safety. In addition, we provide an overview of recent research progress in nutrigenomics, precision nutrition and the interactions occurring in gut microbiota associated with the Nrf2-ARE signaling pathway and diabetes chemoprevention by phytochemicals and finally propose a future research strategy for regulating redox and microbiota balance via the Nrf2-ARE pathway. The present review aims to help us comprehensively understand the critical chemopreventive role of the Nrf2-ARE pathway targeted by phytochemicals in diabetes.
语种:
英文
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蒸汽爆破预处理油菜籽的多酚得率及抗氧化活性
作者:
丰程凤;崔文玉;夏智慧;罗凯云;程安玮
期刊:
湖南农业大学学报(自然科学版) ,2023年49(05):609-615 ISSN:1007-1032
作者机构:
[丰程凤; 崔文玉; 夏智慧; 罗凯云; 程安玮] 湖南农业大学食品科学技术学院
关键词:
油菜籽;菜籽饼;菜籽油;蒸汽爆破;多酚;抗氧化活性
摘要:
油菜籽经0.4、0.7、1.0 MPa等3 种压力及维压1、3 min 的蒸汽爆破预处理后,采用低温压榨法分离菜籽饼及油,分别测定其多酚得率、组成及抗氧化活性,探究蒸汽爆破预处理对其的影响.结果表明:相同维压时间下提高爆破压力或相同的压力下延长维压时间,制备的菜籽油颜色逐渐加深;经蒸汽爆破处理后菜籽饼多酚得率为7.12~8.42 g/(100 g),菜籽油多酚得率显著提高,1.0 MPa下维压3 min 时,菜籽油多酚得率最高,为0.56 g/(100 g);菜籽饼中生成了2,6-二甲氧基4乙烯基苯酚(canolol)、儿茶素、咖啡酸等物质,其中canolol 的转化生成量最大,且一部分迁移到菜籽油中,0.7 MPa下维压3 min 时,菜籽饼和油中canolol含量达到最大,分别为12 120.00、4680.00 mg/kg;DPPH、ABTS 和FRAP 法测定的菜籽油提取物的抗氧化能力显著提升,但菜籽饼的抗氧化能力变化幅度较小;多酚得率与提取物的抗氧化能力之间存在显著或极显著的正相关.可见,蒸汽爆破可作为提高菜籽油中多酚含量及抗氧化活性的一种有效预处理技术,蒸汽压力0.7 MPa、维压3 min 可作为一个较优的汽爆条件用于油菜籽的加工处理.
语种:
中文
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“新医科”建设背景下“临床药物治疗学”新型教学体系建设
作者:
郑郁;刘英姿;冯星;李跑
期刊:
科技风 ,2023年(07):119-121,127 ISSN:1671-7341
作者机构:
湖南师范大学医学院 湖南长沙 410006;湖南农业大学食品科学技术学院 湖南长沙 410128;[李跑] 湖南农业大学;[郑郁; 冯星; 刘英姿] 湖南师范大学
关键词:
临床药物治疗学;全程互动式案例教学;新医科;教学体系
摘要:
"新医科"建设背景下,传统的"临床药物治疗学"教学体系已经不能满足复合型***医学拔尖创新人才培养需求.采用"全程互动式案例教学"模式,通过改革课前准备、课堂教学和课后评价等环节,建立"临床药物治疗学"新型教学体系,利用丰富生动的案例将"新医科"落到实处,以案例为导向、学生为中心,改革了原有的单向输出式教学方式,激发了学生主动学习的能力和热情,为构建"临床药物治疗学"新型教学体系提供一条科学有效的新路径.
语种:
中文
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高压电场杀菌技术研究进展
作者:
邝金艳;林颖;王丽;李宗军
期刊:
肉类研究 ,2023年37(09):52-59 ISSN:1001-8123
作者机构:
湖南农业大学食品科学技术学院,湖南 长沙 410128;[李宗军; 林颖; 王丽; 邝金艳] 湖南农业大学
关键词:
高压脉冲电场;高压静电场;杀菌机理;食品杀菌
摘要:
高压电场杀菌技术具有良好的应用前景,已在食品、卫生等领域受到越来越多的关注.本文介绍2 种常见的高压电场杀菌技术的设备、杀菌机理和影响杀菌的相关因素,综述高压电场杀菌技术在不同电场条件下的杀菌效果和高压电场技术在食品(肉类和水产品)杀菌中的应用,并探讨高压电场技术的发展前景和高压电场技术联用其他杀菌技术的杀菌效果,旨在为高压电场技术在食品杀菌领域中的应用提供参考.
语种:
中文
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采后晾晒及碱液处理对黄精质量和抗氧化活性的影响
作者:
朱晓慧;王锋;苏小军;李清明;郭红英;...
期刊:
食品研究与开发 ,2023年44(08):38-45 ISSN:1005-6521
作者机构:
[王锋; 朱晓慧; 郭红英] 湖南农业大学食品科学技术学院,湖南长沙410128;[王锋; 朱晓慧; 郭红英] 新化县绿源农林科技有限公司,湖南新化417600;[邓文艺] 湖南坤乾食品有限公司,湖南邵东422800;[孙翟翎] 新化县绿源农林科技有限公司;[苏小军; 李清明] 湖南农业大学
关键词:
黄精;采后晾晒;碱液处理;抗氧化;抗糖基化
摘要:
以多花黄精为试材,比较碱液处理及晾晒不同时间(0、3、6、9 d)对黄精质量及抗氧化和抗糖基化活性的影响。主要测定黄精呼吸速率、薯蓣皂苷元、醇浸出物、多糖、黄酮、多酚含量,对黄精提取物进行体外抗氧化活性评价,并测定其对体外糖基化终产物(advanced glycation end-products,AGEs)形成的抑制能力。结果表明,黄精水提物抗氧化和抗糖基化能力显著强于醇提物。采后晾晒导致黄精醇浸出物含量、黄酮、多酚含量下降,多糖含量下降但效果不显著,晾晒9 d后多糖、醇浸出物含量下降了14.4%,醇提物黄酮含量下降了64.6%,多酚含量下降了36.4%,水提物黄酮含量下降了17.1%,多酚含量下降了27.2%,伴随着其提取物抗氧化及抗糖基化能力的下降。碱液处理操作简便,根须脱除效果好且对黄精质量无显著影响,具有应用价值。
语种:
中文
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蕨菜提取液活性物质和抗氧化特性研究
作者:
张茜璐;肖愈;侯爱香
期刊:
中国食物与营养 ,2023年29(06):45-50 ISSN:1006-9577
作者机构:
[张茜璐] 湖南农业大学食品科学技术学院;国家植物功能成分利用工程技术研究中心;[肖愈; 侯爱香] 湖南农业大学食品科学技术学院<&wdkj&>国家植物功能成分利用工程技术研究中心
关键词:
蕨菜;活性物质;抗氧化;相关性
摘要:
目的:比较蕨菜提取物的抗氧化特性,探究不同活性成分与蕨菜提取物抗氧化能力的相关性。方法:以蕨菜水提物和醇提物为原料,测定了其总黄酮、总酚和可溶性糖的含量,同时测定了蕨菜提取液的DPPH·、ABTS+·、·OH的清除能力和总还原能力。结果:试验浓度0.2~1.0 g/mL范围内,在同浓度下,蕨菜水提液的抗氧化综合评价值均大于蕨菜醇提液,预示蕨菜水提液抗氧化能力大于蕨菜醇提液。蕨菜提取液抗氧化综合评价值与蕨菜提取液中总黄酮含量的相关系数为0.911(P<0.01)、与蕨菜提取液中总酚含量的相关系数为0.730(P<0.05)、与蕨菜提取液中可溶性糖含量的相关系数为-0.456。结论:总黄酮含量、总酚含量和蕨菜提取液的抗氧化能力相关性更强,蕨菜中的黄酮物质与酚类物质在抗氧化方面起到协同作用,其中黄酮类物质对蕨菜提取液的抗氧化性有较多贡献,酚类物质次之。
语种:
中文
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挤压高膳食纤维重组米品质评价模型的建立
作者:
何彪;袁书鑫;吴卫国;廖卢艳;邱展英;...
期刊:
北京农学院学报 ,2023年38(04):110-115 ISSN:1002-3186
作者机构:
[何彪; 袁书鑫; 吴卫国; 廖卢艳; 邱展英; 张喻] 湖南农业大学食品科学技术学院
关键词:
膳食纤维;重组米;主成分分析;品质评价模型
摘要:
【目的】为准确评价高膳食纤维重组米品质。【方法】设计了以碎米为主料,大豆膳食纤维和抗性糊精为辅料的16种高膳食纤维重组米配方,采用双螺杆挤压技术制得重组米后测定其蒸煮品质、色泽特性、质构特性共计11项指标,并用感官评价法进行食用品质评价。通过主成分分析建立高膳食纤维重组米品质评价模型,并用该模型对16种高膳食纤维重组米品质进行评价。【结果】结果表明:16种挤压高膳食纤维重组米各项指标变异系数差异大,各品质指标之间存在不同程度的相关性。由主成分分析可将选取的11项指标归纳为3个主成分,其累计方差贡献率为84.155%。【结论】用高膳食纤维重组米的感官评分对建立的模型进行检验,两者具有很好的一致性,表明所建立的模型可为高膳食纤维重组米品质评价提供科学依据。
语种:
中文
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Effect and mechanism of edible oil co-digestion on the bioaccessibility and bioavailability of ursolic acid
作者:
Liu, Yugang;Xia, Huiping;Guo, Shiyin;Li, Peiwang;Qin, Si;...
期刊:
Food Chemistry ,2023年423:136220 ISSN:0308-8146
通讯作者:
Shi, Meng;Zeng, CX
作者机构:
[Zeng, Chaoxi; Zeng, CX; Xia, Huiping; Qin, Si; Shi, Meng; Liu, Yugang; Guo, Shiyin] Hunan Agr Univ, Coll Food Sci & Technol, Dept Food Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Zeng, Chaoxi; Guo, Shiyin] Hunan Agr Univ, Hunan Rapeseed Oil Nutr Hlth & Deep Dev Engn Techn, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Li, Peiwang] Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, 658 Shaoshan South Rd, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Zeng, CX ; Shi, M] H;Hunan Agr Univ, Coll Food Sci & Technol, Dept Food Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词:
Bioaccessibility;Bioavailability;Caco-2 cell monolayer;Simulated digestion in vitro;Ursolic acid
摘要:
Ursolic acid (UA), a pentacyclic triterpenoid, has gained attentions due to its various health-promoting benefits, but exhibits poor bioavailability. This could be enhanced by changing the food matrix of UA in which it is present. In this study, several UA systems were constructed to investigate the bioaccessibility and bioavailability of UA in combination with in vitro simulated digestion and Caco-2 cell models. The results showed that the bioaccessibility of UA was significantly improved after adding rapeseed oil. Caco-2 cell models showed that the UA-oil blend was more advantageous than UA emulsion in total absorption. The results indicate that the location of UA distribution in oil determines the ease of UA release into the mixed micellar phase. This paper brings a new research idea and basis for the design of improving the bioavailability of hydrophobic compounds. © 2023 Elsevier Ltd
语种:
英文
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Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides [辐 射 诱 变 贝 莱 斯 芽 孢 杆 菌 及 其 对 辣 椒 炭 疽 病 抑 菌 效 果]
作者:
谢慧;刘安;陈亮;武小芬;齐慧;...
期刊:
辐射研究与辐射工艺学报 ,2023年41(3):93-103 ISSN:1000-3436
通讯作者:
Liu, S.
作者机构:
[谢慧; 刘素纯] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410125, China;[武小芬; 陈亮; 王克勤; 邓明; 齐慧; 王振; 张祺玲; 刘安] Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha, 410125, China
通讯机构:
[Liu, S.] C;College of Food Science and Technology, China
关键词:
贝莱斯芽孢杆菌;辣椒炭疽病菌;60Co-γ射线辐射诱变;电子束辐射诱变;抑菌
摘要:
采用60Co-γ射线和电子束辐射诱变贝莱斯芽孢杆菌选育遗传稳定的突变菌株,用平板对峙法及四点法对突变菌株进行初筛和复筛,研究其对辣椒炭疽病菌的抑菌效果。结果表明:菌悬液经60C...展开更多 采用60Co-γ射线和电子束辐射诱变贝莱斯芽孢杆菌选育遗传稳定的突变菌株,用平板对峙法及四点法对突变菌株进行初筛和复筛,研究其对辣椒炭疽病菌的抑菌效果。结果表明:菌悬液经60Co-γ射线辐照,贝莱斯芽孢杆菌D10值为2366 Gy,在100~2000 Gy诱变剂量范围内芽孢杆菌致死率随着辐照吸收剂量的增加而升高。在2000 Gy辐照吸收剂量下,贝莱斯芽孢杆菌的致死率为81.8%,并筛选出5株(B004、B112、B114、B117、B118)突变菌株,其对辣椒炭疽病菌抑制率由出发菌株的51.2%分别提高到57.5%、58.0%、57.0%、57.7%和59.9%;贝莱斯芽孢杆菌菌悬液经电子束(10 MeV)辐照,贝莱斯芽孢杆菌D10值为499 Gy,在100~600 Gy诱变剂量范围内,芽孢杆菌致死率随着辐照吸收剂量的增加而升高,在400 Gy辐照吸收剂量下,贝莱斯芽孢杆菌致死率为91.8%,筛选出2株(D115和D243)突变菌株,其对辣椒炭疽病菌抑制率由出发菌株的55.2%分别提高到58.4%和58.1%;B004、B112、B117、B118贝莱斯芽孢杆菌诱变菌株对辣椒炭疽病菌抑菌效果在6代内遗传稳定。研究结果表明:γ射线和电子束辐照在贝莱斯芽孢杆菌的诱变育种中具有潜在的应用价值,本研究可为贝莱斯芽孢杆菌的诱变育种提供基础,为辣椒炭疽病病害的生物防治提供理论参考。收起
语种:
中文
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Properties investigations of rape stalks fermented by different salt concentration: Effect of volatile compounds and physicochemical indexes
作者:
Zhang, Sijie;Li, Congcong;Wu, Junling;Peng, Simin;Wu, Weiguo* ;...
期刊:
Food Chemistry: X ,2023年18:100746 ISSN:2590-1575
通讯作者:
Wu, Weiguo;Liao, LY
作者机构:
[Wu, Junling; Zhang, Sijie; Li, Congcong; Wu, Weiguo; Liao, Luyan; Peng, Simin] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[Wu, Weiguo; Liao, Luyan] 1, Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Wu, WG; Liao, Luyan] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[Wu, WG; Liao, Luyan] 1, Nongda Rd, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wu, WG; Liao, LY ] ;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;1, Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词:
Fermented vegetables;Natural fermentation;Antioxidant components;Free amino acids;Characteristic volatile components;Cluster analysis
摘要:
In order to find out the effect of salt concentration on fermented rape stalks, the physicochemical quality and volatile components was investigated using high performance liquid chromatography (HPLC) and headspace solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC–MS). The results showed that there were abundant kinds of free amino acids (FAAs) in all samples, mainly presenting sweet, umami and bitter taste. Through taste activity value (TAV), His, Glu, and Ala contributed significantly to the taste of the sample. 51 volatile components were identified, of which the relative contents of ketones and alcohols were high. By the relative odor activity value (ROAV) analysis, the main components that had a great impact on the flavor were phenylacetaldehyde, β-Ionone, ethyl palmitate and furanone. Adjusting the appropriate salt concentration for fermentation could improve the comprehensive quality of fermented rape stalks and promote the development and utilization of rape products. © 2023 The Author(s)
语种:
英文
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