关键词：
Dongting Lake area;Ecological environment;Zoning control;Influencing factors;ecological restoration
摘要：
IntroductionProtecting the ecosystem of the Dongting Lake area is of utmost importance for maintaining ecological balance and achieving human well-being.MethodsThis study identifies the key factors influencing the remote sensing based ecological index (RESI) in the Dongting Lake area based on the spatial and temporal evolution characteristics of the RESI and environmental changes and anthropogenic disturbance factors. The priority zoning for ecological restoration was delineated in combination with the anthropogenic composite index (ACI). By exploring the influence of anthropogenic disturbances on RESI, the zoning locations were determined using spatial statistics and linear regression methods.ResultsThe overall RESI of the Dongting Lake area showed a decline from 2001 to 2020, with the mean value decreasing from 0.52 to 0.48. High-quality zones were mainly located in mountainous and forested areas, while low-quality zones were mainly distributed in more developed cities in the east urban area. Anthropogenic factors were the main reasons for the decline in the ecological environment, while natural factors showed a positive correlation with RESI. Based on the RESI and ACI, four ecological control zones (H-H, H-L, L-H, and L-L) were delineated, which accounted for a total of 45.66% of the Dongting Lake area. Among them, 3.91% required immediate control and management, while 17.80% required artificial maintenance. This study explores the influencing factors and mechanisms of the ecological environment quality in the Dongting Lake area, and explores the effective spatial paths for the implementation of ecological restoration zoning control and differentiated restoration strategies in the Dongting Lake area.DiscussionThis study provides a scientific basis for mitigating ecological and environmental problems in the Dongting Lake area, and provides a reference for ecological restoration and regulation and the realization of sustainable development goals in China and global regions with complex environmental problems.
关键词：
Land green use efficiency;digital transformation;Resource-based cities;"Broadband China" policy;Stochastic frontier analysis
摘要：
Introduction: Improving land use efficiency is a necessary way to promote sustainable urban development. The objective of this study is to examine the issue of land green use efficiency (LGUE) in resource-based cities and analyze the impact of digital transformation on LGUE. The research utilizes data from 113 resource-based cities in China spanning from 2008 to 2020.Methodology: Firstly, using the stochastic frontier analysis (SFA) to measure LGUE in China cities, this paper investigates the spatial and temporal evolution of LGUE in different cities. Then, this paper explores the impact of digital transformation on the LGUE of resource-based cities based on the broadband China policy using the DID method.Findings: According to the data measured by the SFA, it is found that resource-based cities are 20.4% lower than non-resource-based cities. An in-depth study found that digital transformation significantly improves LGUE, and the effect is greater in resource-based cities, which is a powerful tool to solve the difficulty of LGUE in resource-based cities. Specifically, digital transformation helps to improve the LGUE of resource-based cities enhance by technological innovation and industrial structure upgrading, and alleviating land factor mismatch.Discussion: It is not only necessary to guide and encourage the in-depth integration of traditional industries with digital technology to enhance the digital transformation of resource-based cities, but also to continuously optimize the allocation of land resources between regions and narrow the gap in LGUE between cities.
关键词：
Agricultural green production technology adoption;Market incentives;Perceived benefits;Perceived risks;Perceived value;Policy subsidies
摘要：
Understanding the underlying reasons for farmers' AGPT adoption in the context of resource environment tightening and agricultural carbon reduction has become crucial to promote agriculture sustainable development. This paper uses multiple ordered regression model and probit model to test the impact of farmers' perceived value on their adoption of agricultural green production technologies (AGPT) by using the first-hand data from 613 rice farmers in Hunan, and explores the effect and mechanism of policy subsidies and market incentives on the adoption of AGPT, and investigates the differences in the impact of perceived value on the adoption of green technologies in different production stages. The results showed that, (1)farmers' AGPT adoption is significantly affected by their perceived value, in which perceived benefits significantly promotes farmers' AGPT adoption, while the perceived risks is the opposite. But farmers' AGPT adoption in different production stages is influenced by different perceived value. The adoption of soil testing and fertilization technology (STFT) in the pre-production stage is significantly influenced by the perceived benefits; adoption of green pest control technology (GPCT) in the mid-production stage is significantly influenced by both the perceived benefits and the perceived risks; and adoption of straw return technology (SRT) in the post-production stage is significantly influenced by the perceived risks. (2) Farmers' adoption of green technologies can be encouraged by policy subsidies, which also have the moderating effect of decreasing the negative influence of perceived risks on farmers' adoption behaviour. (3) Further analysis reveals that market incentives can compensate for the limitations of policy subsidies, greatly promote farmers' adoption of AGPT, and regulate farmers' perceived risks and perceived benefits to encourage them to use green technologies. Consequently, the Government should actively publicize and organize training on agriculture green technologies, and provide diversified subsidy programmes for different green technologies. And governments should also improve the quality certification system and the market price mechanism for agricultural products, so as to help farmers adopting green technologies to achieve an increase in their income.
通讯机构：
[Yan, LH; Li, YL ; Yan, LH ] H;Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.;Hunan Bot Garden, Changsha 410128, Peoples R China.;Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Changsha 410005, Peoples R China.;Minist Educ, Engn Res Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.
关键词：
salt stress;Machilus faberi Hemsl;adaptability;plant growth response;physiological indicators;biochemical indicators
摘要：
Adversity stress is the main environmental factor limiting plant growth and development, including salt and other stress factors. This study delves into the adaptability and salt tolerance mechanisms of Machilus faberi Hemsl, a species with potential for cultivation in salinized areas. We subjected the plants to various salt concentrations to observe their growth responses and to assess key physiological and biochemical indicators. The results revealed that under high salt concentrations (500 and 700 mmol(-1)/L), symptoms such as leaf yellowing, wilting, and eventual death were observed. Notably, plant height and shoot growth ceased on the 14th day of exposure. Chlorophyll content (a, b, total a + b, and the a/b ratio) initially increased but subsequently decreased under varying levels of salt stress. Similarly, the net photosynthetic rate, stomatal conductance, leaf water content, and root activity significantly declined under these conditions. Moreover, we observed an increase in malondialdehyde levels and relative conductivity, indicative of cellular damage and stress. The activity of superoxide dismutase and ascorbate peroxidase initially increased and then diminished with prolonged stress, whereas peroxidase activity consistently increased. Levels of proline and soluble protein exhibited an upward trend, contrasting with the fluctuating pattern of soluble sugars, which decreased initially but increased subsequently. In conclusion, M. faberi exhibits a degree of tolerance to salt stress, albeit with growth limitations when concentrations exceed 300 mmol(-1)/L. These results shed light on the plant's mechanisms of responding to salt stress and provide a theoretical foundation for its cultivation and application in salt-affected regions.
期刊：
Journal of Environmental Management,2024年352:119851 ISSN：0301-4797
通讯作者：
Zhu, ML
作者机构：
[Xu, Junbing] Minjiang Univ, NewHuadu Business Sch, Fuzhou City, Fujian Province, Peoples R China.;[Zhu, Minling] Hunan Agr Univ, Econ Coll, Changsha City, Hunan Province, Peoples R China.;[Zhan, Shaoguo] Chinese Acad Social Sci, Inst Chinese Borderland Studies, Beijing City, Peoples R China.;[Xu, Junbing] Area 1,200,Xiyuangong Rd, Fuzhou City, Fujian Province, Peoples R China.;[Zhu, Minling] Hunan Agr Univ, Coll Agr, Room 219,North 10th Teaching Bldg,1 Yuanda Rd, Changsha City 410125, Hunan Province, Peoples R China.
通讯机构：
[Zhu, ML ] H;Hunan Agr Univ, Econ Coll, Changsha City, Hunan Province, Peoples R China.
摘要：
With global warming, cities are vulnerable to extreme weather, increasing the climate risk to cities worldwide. Although existing literature has examined the ex-post impacts of extreme weather, it is less clear how climate risk affects cites before extreme weather occur. To lower the risk of urban waterlogging, which is caused by extreme weather, and improve the ability of cities to adapt to extreme weather, China launched the Sponge City Project (SCP) in 2013 to manage the urban stormwater and waterlogging. Adopting the SCP pilot in China as a quasi-natural experiment, we examine the impact of the climate risk caused by urban stormwater and waterlogging on the house price with the difference-in-differences (DID) method. We find that after implementing the SCP pilot program, the house price in pilot cities increased significantly because of the improvement in cities' resilience to climate risk. Additionally, this effect was only demonstrated in cities with a high waterlogging risk. For SCP pilot cities with lower waterlogging risk, the house price is not significantly affected by SCP implementation. This indicates that the house price in China is sensitive to the climate risk caused by the urban stormwater and waterlogging. Our findings also contribute to the understanding of the significance of the climate risk management, and provided theoretical evidence for urban governance.
期刊：
FRONTIERS IN ECOLOGY AND EVOLUTION,2024年11:1342766 ISSN：2296-701X
通讯作者：
Zeng, FS
作者机构：
[Zeng, Fusheng; Tan, Fenglian; Lu, Zhaoyan] Hunan Agr Univ, Econ Coll, Changsha, Peoples R China.;[Zeng, Fusheng; Tan, Fenglian; Lu, Zhaoyan] Changsha Univ, Malanshan New Media Coll, Changsha, Peoples R China.
通讯机构：
[Zeng, FS ] H;Hunan Agr Univ, Econ Coll, Changsha, Peoples R China.;Changsha Univ, Malanshan New Media Coll, Changsha, Peoples R China.
关键词：
ecosystem services;trade-offs effect;Geographical detector;Influencing factors;Hilly areas of southern China
摘要：
Introduction This study aims to investigate the factors influencing ecosystem service trade-offs/synergies (TOSs) in major agricultural production areas in the southern hilly region and to propose optimization strategies to promote ecosystem sustainability in agricultural areas.Methods The study used a geographical detector to analyze the determinants influencing the ecosystem service trade-offs as well as correlation analysis, geographically weighted regression (GWR), and a geographical detector to analyze the spatial and temporal evolution of ecosystem service and TOS relationships from 2000 to 2020 in Hunan Province.Results The results showed that the comprehensive value of ecosystem services in Hunan Province showed an increasing trend from 2000 to 2020. With spatial heterogeneity, the areas with high values were mainly distributed in the hilly areas in the west, south, and east of Hunan Province, and the areas with low values were mainly distributed in the Dongting Lake Plain and the Xiangzhong Hilly Basin. There was a trade-off relationship between food production (FP) and all other ecosystem services, of which FP has the strongest trade-off effect with habitat quality (HQ). The synergy effect between HQ, water yield (WY), carbon storage (CS), and soil conservation (SC) shows an increasing trend. Gross domestic product (GDP) and SLOPE are the dominant factors for the strength of trade-offs between food supply and other ecosystem services, and Digital Elevation Model (DEM) and Normalized Difference Vegetation Index (NDVI) are the dominant factors for the strength of synergy effects among ecosystem services. The strength of TOS effects of ecosystem services is determined by interactions or co-influences between the two services rather than by a single component.Discussion The results of this study can provide a reference basis for the enhancement of ecosystem services and the sustainable planning of agricultural landscapes in the southern hilly areas.
关键词：
agricultural green transformation;food security;sustainable development;qualitative comparative analysis
摘要：
In light of the increasing global food crisis, this study concentrated on the complex causality of sustainable food security in China. In the context of the agricultural green transformation, a comprehensive evaluation system of agricultural green development is constructed on China Yearbooks' economic data and agricultural greening indices from 2012 to 2020. In addition, the coupling coordination degree model and fuzzy-set quantitative analysis are used to describe the path evolution of sustainable food security development in China. The results revealed that: (1) the comprehensive assessments were increased in recent years, and high score regions changed apparently, from the periphery to midland; (2) China's green development and agricultural economic potential are currently in a transitional phase from basic to moderate synergy, and the higher coordinated degree is allocating to mid and southern areas during this period, and all of them keeps growing as well; and (3) under modern food security framework, the emphasis of China has gradually shifted from grain output and subsidy policies to high resources utilisation and human capital accumulation. Thus, China's agricultural green transformation and sustainable food security are mutually reinforcing.
期刊：
POLISH JOURNAL OF ENVIRONMENTAL STUDIES,2023年32(6):5333-5346 ISSN：1230-1485
通讯作者：
Zhou, FM
作者机构：
[Tang, Wang; Zhou, Faming; Zhou, FM] Hunan Agr Univ, Econ Coll, Changsha 410128, Hunan, Peoples R China.;[Tang, Wang; Huang, Kunpeng] Hunan Univ Humanities Sci & Technol, Loudi 417000, Hunan, Peoples R China.;[Zhou, Faming; Zhou, FM] Hunan First Normal Univ, Business Sch, Changsha 410205, Hunan, Peoples R China.
通讯机构：
[Zhou, FM ] H;Hunan Agr Univ, Econ Coll, Changsha 410128, Hunan, Peoples R China.;Hunan First Normal Univ, Business Sch, Changsha 410205, Hunan, Peoples R China.
关键词：
high-standard farmland construction policy;agricultural green development;difference-in-difference;PSM-DID;agricultural socialized services;land scale management;quasi-natural experiment
摘要：
Improving agricultural green development (AGD) has become an urgent choice for developing countries to achieve sustainable development and ensure food security. High standard farmland construction (HSFC) has improved agricultural production conditions and ecological environment through artificial improvement of farmland facilities. This study aims to evaluate the role of high standard farmland construction policy in improving agricultural green development. Based on data of rural areas in Hunan, China, this study explores quantitative impacts of HSFC on AGD by difference-in-difference model. The results are as follows: (1) The policy will significantly promote the AGD, and the results remain significant after a series of robustness tests, such as PSM-DID. (2) Mechanism analysis indicates that policies primarily promote AGD through two paths: promoting agricultural socialized services and land-scale management. (3) The results of the heterogeneity analysis indicate that differences in the driving effects of policies are present at different levels of AGD, and the policy effect is stronger in areas with high farmer income levels, large per capita arable land, and in non-grain-producing areas than in other areas. The conclusions of this study provide new empirical evidence and implementation path for developing countries to promote agricultural green development.
作者机构：
[Song, Fengmei; Tang, Xiaomin; Cao, Yi; Zhao, Weichao] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Huang, Chaobo] Nanjing Forestry Univ NFU, Coll Chem Engn, Nanjing 210037, Peoples R China.;[Dai, Xuyan] Hunan Agr Univ, Econ Coll, Changsha 410128, Peoples R China.
通讯机构：
[Yi Cao] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
通讯机构：
[Li, YL ] H;Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.;Hunan Acad Agr Sci, Hunan Hort Res Inst, Changsha 410125, Peoples R China.;Kunpeng Inst Modern Agr, Foshan 528226, Peoples R China.
关键词：
anthocyanins;flavonoid biosynthesis;flower coloring;Loropetalum chinense var. rubrum;metabolomic;transcriptomic
摘要：
The Loropetalum chinense and Loropetalum chinense var. rubrum are typical as well as traditional ornamental and Chinese herbal medicines in Asia; however, more information is needed on the mechanisms underlying their flower coloring. Here, we profiled the flavonoid metabolome and carried out full-length sequencing in addition to transcriptome analyses to investigate the flavonoid biosynthesis and global transcriptome changes among different petal coloring cultivars of L. chinense and L. chinense var. rubrum. The total anthocyanins in addition to the RHSCC values and CIE 1976 L*a*b* values of petals were highly consistent with petal color. Moreover, a total of 207 flavonoid components were identified. Of these, 13 flavonoid compounds were considered significantly different expression compounds highly consistent with color information in the 4 samples. Meanwhile, the first reference full-length transcriptome of L. chinense var. rubrum was built, which had 171,783 high-quality nonredundant transcripts with correcting with next-generation sequencing (NGS). Among them, 52,851 transcripts were annotated in the seven databases of NR, KOG, GO, NT, Pfam, Swiss-Port, and KEGG. Combined with NGS analyses, the DETs involved in flavonoids and anthocyanins contributed greatest to the flower coloring. Additionally, the different expressions of eight LcDFRs and four LcANS genes were positively correlated with flavonoid biosynthesis, and the four LcBZ1 as well as one Lc3Mat1 were positively correlated with the content of seven anthocyanins revealed by coupling with metabolomics and transcriptomics analyses. Together, these results were used to mine candidate genes by analyzing flower coloring changes at comprehensive metabolic and transcriptomic levels in L. chinense and L. chinense var. rubrum.
通讯机构：
[Zhou, F ] H;Hunan Agr Univ, Sch Econ, Changsha 410000, Peoples R China.;Suzhou Univ, Sch Management, Suzhou, Peoples R China.
关键词：
agricultural green development;evaluation framework;regional disparities;spatial dynamics;evolutionary trends
摘要：
Green development is a concept of sustainable development, aiming to protect the environment and ecosystems while meeting economic development needs. In the field of agriculture, green development has emerged as a crucial pathway for reconciling the conflicts between agricultural development and ecological conservation. To investigate the level of green development in Chinese agriculture, regional variations, and the evolutionary patterns, this paper is based on the framework of sustainable development theory. This study establishes a comprehensive evaluation system for agricultural green development and applies methods such as entropy-weighted TOPSIS, Dagum’s Gini coefficient, kernel density estimation, Moran’s I index, and Markov chains to analyze the level of agricultural green development, regional disparities, and dynamic evolution in China. The findings of this study reveal that: (1) The overall level of agricultural green development in China is steadily improving, with notable differences in the level of agricultural green development among different regions and provinces. There are significant disparities in agricultural green development between regions, and the overall disparities exhibit a fluctuating downward trend characterized by periods of increase followed by decrease. The regional disparities are identified as the primary cause of the overall disparities in agricultural green development in China. (2) The eight major economic regions in China are experiencing steady development in agricultural green practices, but there are varying degrees of polarization due to different development speeds. (3) This study also highlights a clear spatial positive correlation in the level of agricultural green development in China, with most provinces showing clustering in the first and third quadrants, indicating a “high–high” (H-H) and “low–low” (L-L) agglomeration pattern. (4) The study reveals that the level of agricultural green development in China exhibits a certain degree of stability. Over time, the probability of transitioning from lower-level regions to neighboring higher-level regions increases, and the agricultural green development level in neighboring regions can influence the spatial transfer probability within a given region. Therefore, agricultural green development demonstrates significant spatial dependence.
通讯机构：
[Lu, Shengrong] C;Center for Macroeconomic Research of Xiamen University, School of Economics, Xiamen University, Xiamen City, Fujian Province, 361005, China.
通讯机构：
[He, H ] H;Hunan Univ Sci & Engn, Res Inst Rural Revitalizat, Yongzhou 425199, Peoples R China.
关键词：
transportation infrastructure development;rural-urban income gap: urbanization;spatial inequality;spatial Dubin model;intermediary effect model
摘要：
The development of transportation infrastructure plays a pivotal role in the regional economy from multiple dimensions. The aim of this paper is to examine the relationship between transportation infrastructure development and income inequality in urban and rural areas of China. The study utilizes panel data from 30 provinces, spanning the years 2010 to 2020, and employs the spatial Dubin model to measure and test the impact of transportation infrastructure on the urban-rural income gap. Furthermore, an intermediary effect test method is used to investigate the potential mediating effect of urbanization in this relationship. The results indicate that transportation infrastructure has a significantly negative direct, indirect, and total effect on the urban-rural income gap, with the indirect effect being greater than the direct effect. This suggests that transportation infrastructure can effectively reduce income disparities, with a noticeable spatial spillover effect. The level of urbanization plays a significant intermediary effect on the effect of transportation infrastructure on the urban-rural income gap, highlighting the role of transportation infrastructure in improving urbanization and narrowing income disparities. These findings underscore the importance of enhancing both the level of urbanization and cooperation between neighbouring regions in order to maximize the benefits of transportation infrastructure development for reducing income disparities and promoting regional balance in China.
通讯机构：
[Huang, S ] H;Hunan Agr Univ, Coll Econ, Changsha 410128, Hunan, Peoples R China.;Hunan Univ Humanities Sci & Technol, Coll Math & Finance, Loudi 417000, Hunan, Peoples R China.
关键词：
energy saving;food productivity;virtual water;water use efficiency
摘要：
The management of water resources requires a correct understanding of the simultaneous management of food and energy resources. The framework of water–food–energy correlation with the approach of sustainability of resources and uses analyzes the combined management and exploitation of water, food, and energy resources with the help of scenario planning. In addition to sustainability concepts, environmental costs such as the emission of carbon dioxide from fossil fuels and its impact on the environment are also discussed. In this research, according to the five defined indicators and based on the potential of using solar energy and the possibility of exploiting renewable energy sources such as solar energy, various management scenarios have been developed. After examining the virtual water management model developed in the Hunan basin as a case study, the development of the water–food–energy nexus model and its calibration, and four scenarios including improving water use efficiency, energy saving, increasing food productivity, and nexus sustainability were developed. The results showed that the nexus strategy can provide sustainability goals according to the weight of each component. After the combined scenario, improving the efficiency of water consumption can be the component with the highest priority in the decision-making model in dry areas.
作者机构：
[Zhang, Donglin; Liu, Yang; Zhang, DL; Huo, Wenwen; Zhang, Yifan; Xu, Lu; Yang, Yujie; Yu, Xiaoying; Li, Yanlin; Xiong, Xingyao; Li, Yang; Xiang, Lili] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.;[Zhang, Donglin; Liu, Yang; Zhang, DL; Huo, Wenwen; Zhang, Yifan; Xu, Lu; Yang, Yujie; Yu, Xiaoying; Li, Yanlin; Li, Yang; Xiang, Lili] Minist Educ, Engn Res Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.;[Ling, Lin] Hunan Agr Univ, Sch Econ, Changsha 410128, Peoples R China.;[Li, Yanlin; Xiong, Xingyao] Kunpeng Inst Modern Agr, Foshan 528225, Peoples R China.;[Xiong, Xingyao] Chinese Acad Agr Sci, Agr Genom Inst Shenzheng, Shenzhen 518120, Peoples R China.
通讯机构：
[Zhang, DL; Yu, XY; Li, YL ] H;[Zhang, DL ] U;Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.;Minist Educ, Engn Res Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.;Kunpeng Inst Modern Agr, Foshan 528225, Peoples R China.
关键词：
Loropetalum chinense var. rubrum;light quality;leaf color;gene expression;anthocyanin
摘要：
Light quality is a vital environmental signal used to trigger growth and to develop structural differentiation in plants, and it influences morphological, physiological, and biochemical metabolites. In previous studies, different light qualities were found to regulate the synthesis of anthocyanin. However, the mechanism of the synthesis and accumulation of anthocyanins in leaves in response to light quality remains unclear. In this study, the Loropetalum chinense var. rubrum "Xiangnong Fendai" plant was treated with white light (WL), blue light (BL), ultraviolet-A light (UL), and blue light plus ultraviolet-A light (BL + UL), respectively. Under BL, the leaves were described as increasing in redness from "olive green" to "reddish-brown". The chlorophyll, carotenoid, anthocyanin, and total flavonoid content were significantly higher at 7 d than at 0 d. In addition, BL treatment also significantly increased the accumulation of soluble sugar and soluble protein. In contrast to BL, ultraviolet-A light increased the malondialdehyde (MDA) content and the activities of three antioxidant enzymes in the leaves, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), in varying degrees over time. Moreover, we also found that the CRY-like gene, HY5-like gene, BBX-like gene, MYB-like gene, CHS-like gene, DFR-like gene, ANS-like gene, and UFGT-like gene were significantly upregulated. Furthermore, the SOD-like, POD-like, and CAT-like gene expressions related to antioxidase synthesis were found under ultraviolet-A light conditions. In summary, BL is more conducive to reddening the leaves of "Xiangnong Fendai" and will not lead to excessive photooxidation. This provides an effective ecological strategy for light-induced leaf-color changes, thereby promoting the ornamental and economic value of L. chinense var. rubrum.
作者机构：
[Luo, Shuangcheng] Hunan Agr Univ, Sch Econ, Changsha 410128, Peoples R China.;[Yuan, Yangli] Beijing Foreign Studies Univ, Sch Int Relationships & Diplomacy, Beijing 100089, Peoples R China.
通讯机构：
[Shuangcheng Luo] S;School of Economics, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Energy conservation and emission reduction are important ways to cope with global warming. An analysis of energy conservation and emission reduction from the perspective of network infrastructure construction provides an important perspective for the study of sustainable development. Based on the research sample of 263 cities in China from 2006 to 2019, and taking the policy of “Broadband China” as a quasi-natural experiment, this paper uses the double difference model to evaluate the impact of network infrastructure construction on energy conservation and emission reduction. The results show that (1) the construction of network infrastructure can significantly improve the energy utilization rate and reduce carbon emissions intensity, which helps to promote energy conservation and emission reduction. (2) From the perspective of a functional mechanism, on the one hand, network infrastructure construction affects energy conservation and emission reduction through micro-mechanisms such as green technology innovation and energy efficiency. On the other hand, network infrastructure construction also drives the development of the Internet and the digital economy, and promotes energy conservation and emission reduction through macro-mechanisms such as industrial structure and financial development. (3) The heterogeneity analysis shows that network infrastructure construction in non-resource-based cities, eastern regions and low-carbon cities has a greater impact on energy conservation and emission reduction. This study provides a new perspective for achieving low-carbon development goals.
作者机构：
[Zhang, Donglin; Zhang, Yifan; Yu, Xiaoying; Li, Yanlin; Xiong, Xingyao; Liu, Yang; Li, YL] Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Engn Res,Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.;[Lin, Ling] Hunan Agr Univ, Sch Econ, Changsha 410128, Peoples R China.;[Xiong, Xingyao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Li, Yanlin; Xiong, Xingyao; Li, YL] Kunpeng Inst Modern Agr, Foshan 528225, Peoples R China.;[Zhang, Donglin] Univ Georgia, Dept Hort, Athens, GA 30602 USA.
通讯机构：
[Yu, XY; Li, YL ] H;Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Engn Res,Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.;Kunpeng Inst Modern Agr, Foshan 528225, Peoples R China.;Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore.
关键词：
Loropetalumchinense var. rubrum;WRKY;expression pattern;genome-wide analysis;light quality
摘要：
The WRKY gene family plays important roles in plant growth and development, as well as in the responses to biotic and abiotic stresses. Loropetalum chinense var. rubrum has high ornamental and medicinal value. However, few WRKY genes have been reported in this plant, and their functions remain unknown. To explore the roles that the WRKY genes play in L. chinense var. rubrum, we identified and characterized 79 LcWRKYs through BLAST homology analysis and renamed them (as LcWRKY1-79) based on their distribution on the chromosomes of L. chinense var. rubrum. In this way, according to their structural characteristics and phylogenetic analysis, they were divided into three groups containing 16 (Group I), 52 (Group II), and 11 (Group III) WRKYs, respectively. LcWRKYs in the same group have similar motifs and gene structures; for instance, Motifs 1, 2, 3, 4, and 10 constitute the WRKY domain and zinc-finger structure. The LcWRKY promoter region contains light response elements (ACE, G-box), stress response elements (TC-rich repeats), hormone response elements (TATC-box, TCA-element), and MYB binding sites (MBS, MBSI). Synteny analysis of LcWRKYs allowed us to establish orthologous relationships among the WRKY gene families of Arabidopsis thaliana, Oryza sativa, Solanum lycopersicum L., Vitis vinifera L., Oryza sativa L., and Zea mays L.; furthermore, analysis of the transcriptomes of mature leaves and flowers from different cultivars demonstrated the cultivar-specific LcWRKY gene expression. The expression levels of certain LcWRKY genes also presented responsive changes from young to mature leaves, based on an analysis of the transcriptome in leaves at different developmental stages. White light treatment led to a significant decrease in the expression of LcWRKY6, 18, 24, 34, 36, 44, 48, 61, 62, and 77 and a significant increase in the expression of LcWRKY41, blue light treatment led to a significant decrease in the expression of LcWRKY18, 34, 50, and 77 and a significant increase in the expression of LcWRKY36 and 48. These results enable a better understanding of LcWRKYs, facilitating the further exploration of their genetic functions and the molecular breeding of L. chinense var. rubrum.
通讯机构：
[Xie, XX ] H;Hunan Agr Univ, Econ Coll, Changsha 410128, Peoples R China.
关键词：
Environmental regulation;Plastic pollution;High-standard mulch film;Use and recycling behavior
摘要：
The widespread use of ultra-thin mulch films has been considered the root cause of China's difficulties in recycling waste mulch films and their severe residue and pollution. Therefore, environmental regulation to guide farmers to use and recycle high-standard mulch has become a key measure to prevent and control mulch pollution at the source. In this paper, we review environmental regulation measures for mulch pollution and their implementation characteristics in the northwest dry farming restoration region as a case study, and explore their impact on the use and recycling of high-standard mulch. The results show that China's environmental regulations on mulch use and recycling can be classified into three types: guidance, incentives, and restrictions. However, the measures implemented so far primarily consist of guidance, while the restrictive and incentive regulations are inadequate. The percentage of farmers using and recycling high-standard mulch under environmental regulations is relatively high (88.18% and 79.83%, respectively), but there is still room for improvement. The study also reveals that while environmental regulations promote the use of high-standard plastic film and its recycling, not all measures are equally effective. Specifically, the implementation of measures such as benefits publicity, price subsidies, technical training, behavior supervision, and punishment mechanisms can be effective in improving farmers' adoption of high-standard plastic mulch; publicizing the hazards of mulch residue, training on recycling techniques, establishing recycling sites, regulating recycling practices, and penalizing violations can effectively help increase the recycling rate of used mulch film. Finally, this study provides a valuable reference for improving regulatory policies for mulch pollution control.
作者机构：
[Qu, Feng; Huang, Yuanyu; Zhao, Yi; Yang, Ge; Zhang, Xiaomin] Beijing Inst Technol, Adv Res Inst Multidisciplinary Sci, Sch Life Sci, Key Lab Mol Med & Biotherapy,Key Lab Med Mol Sci &, Beijing 100081, Peoples R China.;[Yang, Ge] Chinese Acad Med Sci & Peking Union Med Coll, CAMS Key Lab Antiviral Drug Res, Beijing Key Lab Antimicrobial Agents, Inst Med Biotechnol,NHC Key Lab Biotechnol Antibio, Beijing 100050, Peoples R China.;[Dai, Xuyan] Hunan Agr Univ, Econ Coll, Changsha 410128, Peoples R China.;[Liu, Wenjing] Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Beijing Key Lab Drug Resistance TB Res, Beijing 101125, Peoples R China.
通讯机构：
[Yuanyu Huang; Ge Yang] A;Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory of Molecular Medicine and Biotherapy, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, School of Life Science, Advanced Research Institute of Multidisciplinary Science, Beijing Institute of Technology, Beijing 100081, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory of Molecular Medicine and Biotherapy, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, School of Life Science, Advanced Research Institute of Multidisciplinary Science, Beijing Institute of Technology, Beijing 100081, China<&wdkj&>CAMS Key Laboratory of Antiviral Drug Research, Beijing Key Laboratory of Antimicrobial Agents, NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
摘要：
As a type II transmembrane serine protease, fibroblast activation protein (FAP) is specifically expressed on the surface of fibroblasts associated with a variety of epithelial-derived malignancies such as pancreatic cancer, breast cancer, and colon cancer. It participates in the processes of tumorigenesis, progression, and immunosuppression. FAP constitutes an important target for tumor treatment; however, the current studies on FAP are mainly related to structural characteristics, enzymatic properties, and biological functions, and aptamers of FAP have not been investigated. In this work, by using recombinant human FAP as the target, five candidate aptamers, which are AptFAP-A1, AptFAP-A2, AptFAP-A3, AptFAP-A4, and AptFAP-A5, were selected by capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX), and their secondary structures were predicted to be mainly stem-loop. Moreover, the CE-laser-induced fluorescence (LIF) method was used to determine the equilibrium dissociation constant K-D values between the FAP protein and candidate aptamers, and the K-D value was in the low molar range. Finally, Cy5-labeled aptamers were co-incubated with human pancreatic cancer-associated fibroblasts highly expressing FAP protein, and confocal microscopy imaging showed that aptamer AptFAP-A4 had the highest affinities with the cells. The FAP aptamers screened in this study provide a promising direction for the development of rapid tumor diagnosis and targeted therapy.
