通讯机构:
[Cui, GX ; Liu, XY ] H;Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
关键词:
Cannabidiol;Electrochemical sensing;Gold nanoparticles;Carbon black
摘要:
Cannabidiol (CBD), a significant secondary metabolite of Cannabis sativa L., has pharmacological effects for the treatment of a variety of health conditions, including nerve protection, epilepsy, anti-inflammatory, anti-anxiety, and cancer, which have garnered increasing interest in recent years. Herein, an electrochemical sensing platform was constructed for ultrasensitive determination of CBD, on the base of a glassy carbon electrode (GCE) chemically modified with carbon black (CB) and gold nanoparticles (AuNPs) (CB/AuNPs/GCE). The morphology, structure and electrochemical performance of prepared CB/AuNPs/GCE were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), Ultraviolet-visible spectroscopy (UV-vis), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and electrochemical technologies. The effects of pH, scanning rate, enrichment time, enrichment potential and the speculated mechanism of cannabidiol reaction at modified electrode were studied in detail. Under the optimized conditions, the fabricated sensing platform exhibited a great linear response of 0.42 mu A mu M-1 in the concentration range of 0.25 similar to 50.0 mu M. The prepared CB/AuNPs/GCE showed long-term stability, good repeatability and high practicability in real samples of industrial hemp. The proposed sensor can be further enhanced for portable and rapid detection of plant secondary metabolites, providing a basis for food detection, agricultural development, and drug detection.
摘要:
Allelopathy has been considered a good explanation for the successful invasion of some invasive plants. However, the real latitudinal and longitudinal allelopathic effects on native species have rarely been documented since many exotics have spread widely. We conducted a Petri dish experiment to determine the latitudinal and longitudinal allelopathic patterns of an invasive alligator weed (Alternanthera philoxeroides) on a common crop (Lactuca sativa) in China, and find what determines the allelopathic intensity. The results showed that the allelopathic effects of A. philoxeroides increased with the latitude while decreased with the longitude. This indicated that A. philoxeroides used its allelopathy to gain competitive advantages more in its recent invaded communities than that in its early invaded ones as A. philoxeroides is expanding from southeast China to northwest China. Furthermore, we found that the allelopathic intensity of A. philoxeroide was negatively correlated to the leaf contents of soluble carbohydrate (SC), carbon (C) and nitrogen (N), but that was positively correlated to the leaf contents of soluble protein (SP), free amino acids (FAA), plant polyphenol (PP), phosphorus (P) and potassium (K). These results suggested that the allelopathic intensity of A. philoxeroide was more determined by the limited P and K nutrients as well as the intermediate allelochemicals (SP, FAA, PP) rather than the unlimited C, N and SC. Thus, we can speculate that the negative or positive effects of plant aqueous extracts are a function of not only the extract concentrations but also the trade-offs between inhibition and promotion of all components in the extracts. Then we could reduce the allelopathic effects of A. philoxeroide by controlling the component contents in the plant tissues, by fertilization or other managements, especially in the plant recent invaded communities.
关键词:
gut microbiota;dietary diet;metabolites;short-chain fatty acids;bile acids
摘要:
Type 2 diabetes (T2D) is a commonly diagnosed condition that has been extensively studied. The composition and activity of gut microbes, as well as the metabolites they produce (such as short-chain fatty acids, lipopolysaccharides, trimethylamine N-oxide, and bile acids) can significantly impact diabetes development. Treatment options, including medication, can enhance the gut microbiome and its metabolites, and even reverse intestinal epithelial dysfunction. Both animal and human studies have demonstrated the role of microbiota metabolites in influencing diabetes, as well as their complex chemical interactions with signaling molecules. This article focuses on the importance of microbiota metabolites in type 2 diabetes and provides an overview of various pharmacological and dietary components that can serve as therapeutic tools for reducing the risk of developing diabetes. A deeper understanding of the link between gut microbial metabolites and T2D will enhance our knowledge of the disease and may offer new treatment approaches. Although many animal studies have investigated the palliative and attenuating effects of gut microbial metabolites on T2D, few have established a complete cure. Therefore, conducting more systematic studies in the future is necessary.
通讯机构:
[Tongcheng Fu] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Engineering Laboratory of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, China<&wdkj&>National Energy R&D Center for Non-Food Biomass, Hunan Agricultural University, Changsha, China
关键词:
marginal land;soil aggregate;soil carbon sequestration;soil ecology;soil iron and aluminum oxide;soil microbe
摘要:
Long‐term cultivation of Miscanthus and Saccharum arundinaceum increased soil organic carbon stock in infertile red soils. Soil pH is the main factor affecting soil carbon stock in red soils. Soil copiotrophic and oligotrophic microbes significantly shaped by the cultivation of perennial grasses. Abstract Determining the effect of perennial energy crop (PEC) cultivation on soil organic carbon (SOC) in marginal land soil is vital for carbon neutrality and bioeconomy development. However, a comprehensive and systematic evaluation of the response of SOC content to different PECs and its underlying drivers is still lacking. We used soil data collected from infertile red topsoil (0–20 cm) after 10 years of cultivation with Miscanthus (MS), Panicum virgatum (SG), and Saccharum arundinaceum (SA) to explore the changes in SOC stock induced by PEC. The roles of physical, chemical, and microbiological factors driving the increase in the SOC stock were investigated. Results revealed that SA and MS enhanced SOC stock by 87.97% and 27.52% relative to the uncultivated control. Conversely, PEC increased the percentage of soil mega‐aggregates, geometric mean diameters, soil chelate iron (Fe), and aluminum (Al) oxides, and reduced soil acidity for the infertile red soils. In addition, fungal richness and diversity for PEC soils were enhanced compared to the unplanted soil. It is possible that PEC cultivation reduced the relative abundance of copiotrophic fungi but increased the relative abundance of oligotrophic fungi. Furthermore, variance partitioning analysis revealed that chemical and microbiological factors accounted for 80.54% of the total variation for the SOC stock. The partial least squares path model showed that PEC cultivation enhanced soil carbon (C) stock via soil deacidification and increased soil bacterial function. In conclusion, this study confirms the SOC sequestration potential of PEC cultivation in marginal land and the underlying mechanism driving SOC stock. The main positive factors controlling soil C sequestration included “pH,” while the negative factors were “bacterial community,” “fungal community,” and “bacterial function.” Our research may help encourage and support decision‐makers of wasted marginal land conversion to PEC cultivation.
作者机构:
[Sun, Wei; Gao, Shuai; Li, Shuzhen; Su, Guirong; He, Zhiguo] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Su, Guirong] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[He, ZG; He, Zhiguo] Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;[Zhong, Hui; Zhong, H] Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
通讯机构:
[He, ZG ] A;[Zhong, H ] C;Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
摘要:
Pine oil, commonly used as a flotation frother, could affect the bioleaching process. The attachment and biofilm development of acidophiles on metal sulfides were essential for bioleaching. This study focused on the role of pine oil in the early adhesion and biofilm development by Acidianus manzaensis YN-25 on the chalcopyrite surface. It was revealed that pine oil had a negative effect on the adhesion amount of A. manzaensis on the chalcopyrite surface. After having reached adhesion equilibrium, the percentage of attached cells decreased as the concentration of pine oil increased. The addition of pine oil was found to decrease the negative charge of chalcopyrite and cells, resulting in a higher attraction of acid-base interaction energy and total interaction force. This ultimately led to a stronger adhesion force between chalcopyrite and A. manzaensis. While the addition of pine oil initially delayed the formation of cell microcolonies during the first stage of biofilm formation, which ultimately resulted in larger biofilm coverage compared with the control group. The microcolonies on the mineral surface were also larger and the corrosion pits were bigger with the addition of pine oil. Furthermore, the addition of pine oil weakened the passivation effect and promoted the biological dissolution of chalcopyrite. This study offers a better understanding of the effect of pine oil on the bioleaching of chalcopyrite.
通讯机构:
[Hong-Yuan Zeng] H;[Ai-Ping Wu] E;Ecology Department, College of Resources and Environment, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Institute of Microbiology, Changsha 410009, China
摘要:
Abstract: Water level rise is considered an environmental filter for the growth and reproduction of aquatic plants in lakes. Some emergent macrophytes can form floating mats, enabling them to escape from the negative effects of deep water. However, an understanding of which species can be uprooted and form floating mats easily and what factors affect these tendencies remains greatly elusive. We conducted an experiment to determine whether the monodominance of Zizania latifolia in the emergent vegetation community in Lake Erhai was related to its floating mat formation ability and to try to find the reasons for its floating mat formation ability during the continuous increase in water level over the past few decades. Our results showed that both the frequency and biomass proportion of Z. latifolia were greater among the plants on the floating mats. Furthermore, Z. latifolia was more likely to be uprooted than the other three previously dominant emergent species due to its smaller angle between the plant and the horizontal plane, rather than the root:shoot or volume:mass ratios. The dominance of Z. latifolia in the emergent community in Lake Erhai is due to its easier ability to become uprooted, allowing it to outperform other emergent species and become the single dominant emergent species under the environmental filter of deep water. The ability to uproot and form floating mats may be a competitive survival strategy for emergent species under the conditions of continuous significant water level rise. Keywords: aquatic plant; floating mat; emergent macrophyte; uprooting; angle; environmental filter; deep water; survival strategy
通讯机构:
[Xia, M; Zhou, Z ] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Prov Engn Technol Res Ctr Opt Agr, Changsha 410128, Peoples R China.
关键词:
Phosphor;Luminescence;NIR emission;Bi3+
摘要:
Herein, the strategy of replacing Ge4+ with smaller Si4+ was adopted to realize the site-selective occupation of Bi3+ activator in the small ring and obtain a near-infrared light-emitting in Zn-2(Ge,Si)O-4. The designed phosphor exhibits a broad NIR emission with FWHM approximate to 104 nm in the 650-860 nm region, with a center emission wavelength of about 750 nm. Interestingly, the more sensitive four-member ring sites gradually replaced the six-member ring sites and realized a large-scope photoluminescence regulation from blue to NIR by just after the crystal field engineering. The possible reasons for this phenomenon can be interpreted by centroid shift (epsilon(c)) and crystal field splitting (epsilon(cfs)). This work not only provides new insights for the development of Bi3+-activated NIR-emitting phosphors, but also provides thoughts for revealing the potential NIR luminous mechanism of Bi3+.
摘要:
Chalkiness is a key determinant that directly affects the appearance and cooking quality of rice grains. Previously, Floury endosperm 2 (FLO2) was reported to be involved in the formation of rice chalkiness; however, its regulation mechanism is still unclear. Here, FLO2 interaction factor 3 (OsFIF3), a bHLH transcription factor, was identified and analyzed in Oryza sativa. A significant increase in chalkiness was observed in OsFIF3-overexpressed grains, coupled with a round, hollow filling of starch granules and reduced grain weight. OsFIF3 is evolutionarily conserved in monocotyledons, but variable in dicotyledons. Subcellular localization revealed the predominant localization of OsFIF3 in the nucleus. The DAP-seq (DNA affinity purification sequencing) results showed that OsFIF3 could affect the transcriptional accumulation of β-amylase 1, α-amylase isozyme 2A-like, pectinesterase 11, β-glucosidase 28 like, pectinesterase, sucrose transport protein 1 (SUT1), and FLO2 through the binding of the CACGTG motif on their promoters. Moreover, FLO2 and SUT1 with abundant OsFIF3 binding signals showed significant expression reduction in OsFIF3 overexpression lines, further confirming OsFIF3's role in starch metabolism regulation and energy material allocation. Taken together, these findings show that the overexpression of OsFIF3 inhibits the expression of FLO2 and SUT1, thereby increasing grain chalkiness and affecting grain weight.
作者机构:
[Chen, Zhiqun; Yan, Li; Feng, Guifang] Linyi Univ, Coll Life Sci, Linyi 276000, Peoples R China.;[Feng, Guifang] Philippine Christian Univ, Ctr Int Educ, Manila 1004, Philippines.;[Hao, Xiaodong; Zhu, Ping] Linyi Univ, Coll Resources & Environm, Linyi 276000, Peoples R China.;[Xiao, Yunhua] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构:
[Yunhua Xiao; Xiaodong Hao] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Resources and Environment, Linyi University, Linyi 276000, China
关键词:
Cd-contaminated soils;mixotrophic acidophiles;free and attached communities;microbial diversity;Cd removal
摘要:
Abstract: In this study, cadmium (Cd)-contaminated soils were bioremediated using the mixotrophic acidophiles in different scale-up cultivation stages. The free and attached bacterial and fungal diversities and community structures, and their effects on soil Cd removal were investigated. Percentages of dominant bacteria (Acidithiobacillus, Sulfobacillus, and Pseudomonas) and fungi (Acidomyces and Coniochaeta) between the free and attached communities exhibited the pronounced differences. The α-diversity of attached bacteria was higher than the free bacteria, while the distinct variations in attached and free fungi were not observed. However, the β-diversity between the free and attached bacteria and fungi was significantly changed. Solution pH and reduced glucose level affected the abundance patterns of Acidithiobacillus in free bacteria and Acidomyces in attached fungi, which had great contributions to the soil Cd removal efficiency. Keywords: Cd-contaminated soils; mixotrophic acidophiles; free and attached communities; microbial diversity; Cd removal
摘要:
Abstract: Ribosome assembly factors have been extensively studied in yeast, and their abnormalities may affect the assembly process of ribosomes and cause severe damage to cells. However, it is not clear whether mRNA turnover protein 4 (MRT4) functions in the fungal growth and pathogenicity in Sclerotinia sclerotiorum. Here, we identified the nucleus-located gene SsMRT4 using reverse genetics, and found that knockdown of SsMRT4 resulted in retard mycelia growth and complete loss of pathogenicity. Furthermore, mrt4 knockdown mutants showed almost no appressorium formation and oxalic acid production comparing to the wild-type and complementary strains. In addition, the abilities to ROS elimination and resistance to oxidative and osmotic stresses were also seriously compromised in mrt4 mutants. Overall, our study clarified the role of SsMRT4 in S. sclerotiorum, providing new insights into ribosome assembly in regulating pathogenicity and resistance to environmental stresses of fungi. Keywords: Sclerotinia sclerotiorum; SsMRT4; ribosome assembly; ROS; pathogenicity
摘要:
Microbial technology provides a feasible and effective means for removing tetracycline from water bodies. In this study, a novel tetracycline removal strain was isolated from pig manure and identified as an Alcaligenes sp. The effects of inoculation dose, initial tetracycline concentration, pH and temperature on the tetracycline removal efficiencies of the Alcaligenes sp. R3 strain were investigated. Response surface methodology (RSM) using a Box-Behnken design demonstrated that the optimal conditions for the temperature, pH, inoculation dose of Alcali-genes sp. R3 and initial tetracycline concentration were 32.55 degrees C, 8.83, 17.32% and 54.58 mg/L, respectively. Alcaligenes sp. R3 could remove 71.62% of tetracycline at most. To improve the ability of Alcaligenes sp. R3 to remove tetracycline, it was immobilized with carboxymethylcellulose (CMC) and polydopamine (PDA) to construct immobilized microbe pellets (IMPs); scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) of the IMPs showed that the CMC/PDA exhibited good adsorption ability and biocompatibility and could thus be used as a carrier of the living cells. The maximum tetracycline removal percentage of the IMPs was 91.16%, indicating that the ability of the IMPs to remove tetracycline was superior to that of free cells. Through immobilization with CMC/PDA, IMPs could remove tetracycline more effectively and had better environmental tolerance than free Alcaligenes sp. R3. The identification of possible biodegradation products indicated that the degradation pathway of strain R3 was novel and CMC/PDA only adsorbed tetracy-cline but not have degradation effect on it.
作者:
Joaquín Castro-Montoya;Yasir Iqbal;Moritz von Cossel*
期刊:
Agronomy,2023年13(11):2818- ISSN:2073-4395
通讯作者:
Moritz von Cossel
作者机构:
Biobased Resources in the Bioeconomy (340b), Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Germany;Author to whom correspondence should be addressed.;[Yasir Iqbal] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Joaquín Castro-Montoya] Institute of Agri-Food and Environmental Sciences, Faculty of Agricultural Sciences, University of El Salvador, San Salvador 01101, El Salvador;[Moritz von Cossel] Biobased Resources in the Bioeconomy (340b), Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Germany<&wdkj&>Author to whom correspondence should be addressed.
通讯机构:
[Moritz von Cossel] B;Biobased Resources in the Bioeconomy (340b), Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Germany<&wdkj&>Author to whom correspondence should be addressed.
摘要:
Planet Earth is facing numerous imminent challenges, from climate change to ecological dysfunction, which are largely attributed to anthropogenic activities [...]
摘要:
Cordycepin, a nucleoside compound with a variety of biological activities, has been extensively applied in the nutraceutical and pharmaceutical industries. The advancement of microbial cell factories using agro-industrial residues provides a sustainable pathway for cordycepin biosynthesis. Herein, the cordycepin production was enhanced by the modification of glycolysis and pentose phosphate pathway in engineered Yarrowia lipolytica. Then, cordycepin production based on economical and renewable substrates (sugarcane molasses, waste spent yeast, and diammonium hydrogen phosphate) was analyzed. Furthermore, the effects of C/N molar ratio and initial pH on cordycepin production were evaluated. Results indicated that the maximum cordycepin productivity of 656.27mg/L/d (72h) and cordycepin titer was 2286.04mg/L (120h) by engineered Y. lipolytica in the optimized medium, respectively. The cordycepin productivity in the optimized medium was increased by 28.81% compared with the original medium. This research establishes a promising way for efficient cordycepin production from agro-industrial residues.