通讯机构：
[Shitou Xia] H;Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Ribosome assembly factors have been extensively studied in yeast, and their abnormalities may affect the assembly process of ribosomes and cause severe damage to cells. However, it is not clear whether mRNA turnover protein 4 (MRT4) functions in the fungal growth and pathogenicity in Sclerotinia sclerotiorum. Here, we identified the nucleus-located gene SsMRT4 using reverse genetics, and found that knockdown of SsMRT4 resulted in retard mycelia growth and complete loss of pathogenicity. Furthermore, mrt4 knockdown mutants showed almost no appressorium formation and oxalic acid production comparing to the wild-type and complementary strains. In addition, the abilities to ROS elimination and resistance to oxidative and osmotic stresses were also seriously compromised in mrt4 mutants. Overall, our study clarified the role of SsMRT4 in S. sclerotiorum, providing new insights into ribosome assembly in regulating pathogenicity and resistance to environmental stresses of fungi.
摘要：
From the aerobic pond of the farm, the Pseudomonas sp. G16 was screened and isolated, which was confirmed to exhibit heterotrophic nitrification and aerobic denitrification. The removal rates of Ammonia (100mg/L), nitrate (120mg/L), and nitrite (100mg/L) by the strain were 94.13%, 92.62%, and 85.67%, and the nitrogen metabolism pathway of strain G16 was analyzed by whole genome sequencing combined with its nitrification-denitrification intermediate products, it was found that the strain had independent nitrification-denitrification ability and no nitrite accumulation. Under the conditions of carbon source of sodium succinate hexahydrate, C/N ratio of 15, pH of 7.5, temperature of 15°C, and DO of 210rpm, strain G16 showed excellent denitrification performance. Strain G16 was prepared into biochar-based immobilized bacterial particles, which successfully improved its nitrogen removal efficiency and stability. Therefore, the application of strain G16 in the field of real wastewater treatment has very necessary research value.
通讯机构：
[Tongcheng Fu] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Engineering Laboratory of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, China<&wdkj&>National Energy R&D Center for Non-Food Biomass, Hunan Agricultural University, Changsha, China
关键词：
marginal land;soil aggregate;soil carbon sequestration;soil ecology;soil iron and aluminum oxide;soil microbe
摘要：
Long‐term cultivation of Miscanthus and Saccharum arundinaceum increased soil organic carbon stock in infertile red soils. Soil pH is the main factor affecting soil carbon stock in red soils. Soil copiotrophic and oligotrophic microbes significantly shaped by the cultivation of perennial grasses. Abstract Determining the effect of perennial energy crop (PEC) cultivation on soil organic carbon (SOC) in marginal land soil is vital for carbon neutrality and bioeconomy development. However, a comprehensive and systematic evaluation of the response of SOC content to different PECs and its underlying drivers is still lacking. We used soil data collected from infertile red topsoil (0–20 cm) after 10 years of cultivation with Miscanthus (MS), Panicum virgatum (SG), and Saccharum arundinaceum (SA) to explore the changes in SOC stock induced by PEC. The roles of physical, chemical, and microbiological factors driving the increase in the SOC stock were investigated. Results revealed that SA and MS enhanced SOC stock by 87.97% and 27.52% relative to the uncultivated control. Conversely, PEC increased the percentage of soil mega‐aggregates, geometric mean diameters, soil chelate iron (Fe), and aluminum (Al) oxides, and reduced soil acidity for the infertile red soils. In addition, fungal richness and diversity for PEC soils were enhanced compared to the unplanted soil. It is possible that PEC cultivation reduced the relative abundance of copiotrophic fungi but increased the relative abundance of oligotrophic fungi. Furthermore, variance partitioning analysis revealed that chemical and microbiological factors accounted for 80.54% of the total variation for the SOC stock. The partial least squares path model showed that PEC cultivation enhanced soil carbon (C) stock via soil deacidification and increased soil bacterial function. In conclusion, this study confirms the SOC sequestration potential of PEC cultivation in marginal land and the underlying mechanism driving SOC stock. The main positive factors controlling soil C sequestration included “pH,” while the negative factors were “bacterial community,” “fungal community,” and “bacterial function.” Our research may help encourage and support decision‐makers of wasted marginal land conversion to PEC cultivation.
通讯机构：
[Wei Hou; Wei Hou Wei Hou Wei Hou] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, P.R. China
摘要：
I was invited to make a brief commentary on a recent article titled “Determining Spatially Varying Profit-Maximizing Management Practices for Miscanthus and Switchgrass Production in the Rainfed United States” published in GCBB by Zhang et al. (2022). In the work, they propose management practices to maximize profitability through economically optimal N fertilizer application, temporal and spatial variation, and optimal age rotation of two energy crops. This interesting and thoroughly investigated result would be instructive for the applications of perennial energy crops.
通讯机构：
[Tiean Zhou; Yanyang Wu] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Engineering Technology Research Center for Cell Mechanics and Function Analysis, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
作者：
Robert, Hélène S.;Heisler, Marcus G.;Xiao, Langtao;Landrein, Benoit
期刊：
FRONTIERS IN PLANT SCIENCE,2023年14:1319859 ISSN：1664-462X
作者机构：
Hormonal Crosstalk in Plant Development, Mendel Center for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University (CEITEC MU), Czechia;School of Life and Environmental Sciences, University of Sydney, Australia;College of Bioscience and Biotechnology, Hunan Agricultural University, China;Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon, CNRS, INRAE, INRIA, France
摘要：
IntroductionPhytohormones shape the plant above and below ground to enable grounding, interaction with the soil microbiome and with surrounding plants, water and nutrient uptake, organ production, photosynthesis optimization, and seed production. The plant aerial architecture results from the dynamic processes of shoot branching, leaf and flower production following phyllotaxis, and reproduction that originate from the post-embryonic activity of the shoot apical meristem. Hormones influence and coordinate plant development and physiology at the organism level. Different hormones regulate different aspects of shoot development, acting together or antagonistically. This Research Topic compiles seven articles, e.g., four original research and three review articles, dealing with the influence of hormones on shoot development, branching regulation, flower aging, and fruit maturation. They show that all these processes are influenced by many different hormones, such as ethylene, gibberellins, abscisic acid, auxin, cytokinin, and strigolactones.Shooting and Branching processes - A hub for hormonal crosstalkPost-embryonic shoot organogenesis depends on the activity of the shoot apical meristem (SAM). The SAM contains a pool of slow-growing stem cells in the central zone (CZ), surrounded by fast-growing cells in the peripherical zone (PZ), where organs are initiated. It is structured in three cell layers. The SAM is maintained through the WUSCHEL (WUS) – CLAVATA 3 (CLV3) module (Gr...
通讯机构：
[Zhi Liu] C;[Junhua Chen] N;National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, 510650, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China
摘要：
We successfully constructed several cascaded molecular logic gates (2INHIBIT-2AND, 2AND-2OR, and 2OR-2INHIBIT) using three different antibiotics as the inputs. In the presence of kanamycin (KAN), chloramphenicol (CHL), or oxytetracycline (OXY), the aptamer-antibiotic recognition will release the trigger DNA to active the hairpin DNA hybridization. Exonuclease III (Exo III)-mediated catalysis reaction was introduced in the logic system to generate Mg2+-dependent DNAzyme, which was used to cleave the fluorescence signal reporter probe. For input, the presence and absence of the antibiotic was defined as 1 and 0, respectively. For output, the fluorescence intensity higher or lower than the threshold value was defined as 1 and 0, respectively. In the 2INHIBIT-2AND logic circuit, the 101 input combination generates an output of 1 and other input combinations generate an output of 0. In the 2AND-2OR logic circuit, the input combinations of 001, 011, 110, 101, and 111 generate an output of 1 and other input combinations generate an output of 0. In the 2OR-2INHIBIT logic circuit, the input combinations of 010, 100, and 110 generate an output of 1 and other input combinations generate an output of 0. Our constructed logic system exhibits high selectivity and can work even in complex water samples. With the advantages of multiple biocomputation capabilities, high flexibility, and easy scalability, this logic gate system provides a new analytical method for the intelligent detection of different antibiotics.
摘要：
Microbial technology provides a feasible and effective means for removing tetracycline from water bodies. In this study, a novel tetracycline removal strain was isolated from pig manure and identified as an Alcaligenes sp. The effects of inoculation dose, initial tetracycline concentration, pH and temperature on the tetracycline removal efficiencies of the Alcaligenes sp. R3 strain were investigated. Response surface methodology (RSM) using a Box-Behnken design demonstrated that the optimal conditions for the temperature, pH, inoculation dose of Alcali-genes sp. R3 and initial tetracycline concentration were 32.55 degrees C, 8.83, 17.32% and 54.58 mg/L, respectively. Alcaligenes sp. R3 could remove 71.62% of tetracycline at most. To improve the ability of Alcaligenes sp. R3 to remove tetracycline, it was immobilized with carboxymethylcellulose (CMC) and polydopamine (PDA) to construct immobilized microbe pellets (IMPs); scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) of the IMPs showed that the CMC/PDA exhibited good adsorption ability and biocompatibility and could thus be used as a carrier of the living cells. The maximum tetracycline removal percentage of the IMPs was 91.16%, indicating that the ability of the IMPs to remove tetracycline was superior to that of free cells. Through immobilization with CMC/PDA, IMPs could remove tetracycline more effectively and had better environmental tolerance than free Alcaligenes sp. R3. The identification of possible biodegradation products indicated that the degradation pathway of strain R3 was novel and CMC/PDA only adsorbed tetracy-cline but not have degradation effect on it.
关键词：
gut microbiota;dietary diet;metabolites;short-chain fatty acids;bile acids
摘要：
Type 2 diabetes (T2D) is a commonly diagnosed condition that has been extensively studied. The composition and activity of gut microbes, as well as the metabolites they produce (such as short-chain fatty acids, lipopolysaccharides, trimethylamine N-oxide, and bile acids) can significantly impact diabetes development. Treatment options, including medication, can enhance the gut microbiome and its metabolites, and even reverse intestinal epithelial dysfunction. Both animal and human studies have demonstrated the role of microbiota metabolites in influencing diabetes, as well as their complex chemical interactions with signaling molecules. This article focuses on the importance of microbiota metabolites in type 2 diabetes and provides an overview of various pharmacological and dietary components that can serve as therapeutic tools for reducing the risk of developing diabetes. A deeper understanding of the link between gut microbial metabolites and T2D will enhance our knowledge of the disease and may offer new treatment approaches. Although many animal studies have investigated the palliative and attenuating effects of gut microbial metabolites on T2D, few have established a complete cure. Therefore, conducting more systematic studies in the future is necessary.
作者机构：
[Sun, Wei; Gao, Shuai; Li, Shuzhen; Su, Guirong; He, Zhiguo] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Su, Guirong] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[He, ZG; He, Zhiguo] Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;[Zhong, Hui; Zhong, H] Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
通讯机构：
[He, ZG ] A;[Zhong, H ] C;Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
摘要：
Pine oil, commonly used as a flotation frother, could affect the bioleaching process. The attachment and biofilm development of acidophiles on metal sulfides were essential for bioleaching. This study focused on the role of pine oil in the early adhesion and biofilm development by Acidianus manzaensis YN-25 on the chalcopyrite surface. It was revealed that pine oil had a negative effect on the adhesion amount of A. manzaensis on the chalcopyrite surface. After having reached adhesion equilibrium, the percentage of attached cells decreased as the concentration of pine oil increased. The addition of pine oil was found to decrease the negative charge of chalcopyrite and cells, resulting in a higher attraction of acid-base interaction energy and total interaction force. This ultimately led to a stronger adhesion force between chalcopyrite and A. manzaensis. While the addition of pine oil initially delayed the formation of cell microcolonies during the first stage of biofilm formation, which ultimately resulted in larger biofilm coverage compared with the control group. The microcolonies on the mineral surface were also larger and the corrosion pits were bigger with the addition of pine oil. Furthermore, the addition of pine oil weakened the passivation effect and promoted the biological dissolution of chalcopyrite. This study offers a better understanding of the effect of pine oil on the bioleaching of chalcopyrite.
通讯机构：
[Hong-Yuan Zeng; Ai-Ping Wu] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Institute of Microbiology, Changsha 410009, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Ecology Department, College of Resources and Environment, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha 410128, China
摘要：
Water level rise is considered an environmental filter for the growth and reproduction of aquatic plants in lakes. Some emergent macrophytes can form floating mats, enabling them to escape from the negative effects of deep water. However, an understanding of which species can be uprooted and form floating mats easily and what factors affect these tendencies remains greatly elusive. We conducted an experiment to determine whether the monodominance of Zizania latifolia in the emergent vegetation community in Lake Erhai was related to its floating mat formation ability and to try to find the reasons for its floating mat formation ability during the continuous increase in water level over the past few decades. Our results showed that both the frequency and biomass proportion of Z. latifolia were greater among the plants on the floating mats. Furthermore, Z. latifolia was more likely to be uprooted than the other three previously dominant emergent species due to its smaller angle between the plant and the horizontal plane, rather than the root:shoot or volume:mass ratios. The dominance of Z. latifolia in the emergent community in Lake Erhai is due to its easier ability to become uprooted, allowing it to outperform other emergent species and become the single dominant emergent species under the environmental filter of deep water. The ability to uproot and form floating mats may be a competitive survival strategy for emergent species under the conditions of continuous significant water level rise.
摘要：
Chalkiness is a key determinant that directly affects the appearance and cooking quality of rice grains. Previously, Floury endosperm 2 (FLO2) was reported to be involved in the formation of rice chalkiness; however, its regulation mechanism is still unclear. Here, FLO2 interaction factor 3 (OsFIF3), a bHLH transcription factor, was identified and analyzed in Oryza sativa. A significant increase in chalkiness was observed in OsFIF3-overexpressed grains, coupled with a round, hollow filling of starch granules and reduced grain weight. OsFIF3 is evolutionarily conserved in monocotyledons, but variable in dicotyledons. Subcellular localization revealed the predominant localization of OsFIF3 in the nucleus. The DAP-seq (DNA affinity purification sequencing) results showed that OsFIF3 could affect the transcriptional accumulation of β-amylase 1, α-amylase isozyme 2A-like, pectinesterase 11, β-glucosidase 28 like, pectinesterase, sucrose transport protein 1 (SUT1), and FLO2 through the binding of the CACGTG motif on their promoters. Moreover, FLO2 and SUT1 with abundant OsFIF3 binding signals showed significant expression reduction in OsFIF3 overexpression lines, further confirming OsFIF3's role in starch metabolism regulation and energy material allocation. Taken together, these findings show that the overexpression of OsFIF3 inhibits the expression of FLO2 and SUT1, thereby increasing grain chalkiness and affecting grain weight.
通讯机构：
[Cui, GX ; Liu, XY ] H;Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
关键词：
Cannabidiol;Electrochemical sensing;Gold nanoparticles;Carbon black
摘要：
Cannabidiol (CBD), a significant secondary metabolite of Cannabis sativa L., has pharmacological effects for the treatment of a variety of health conditions, including nerve protection, epilepsy, anti-inflammatory, anti-anxiety, and cancer, which have garnered increasing interest in recent years. Herein, an electrochemical sensing platform was constructed for ultrasensitive determination of CBD, on the base of a glassy carbon electrode (GCE) chemically modified with carbon black (CB) and gold nanoparticles (AuNPs) (CB/AuNPs/GCE). The morphology, structure and electrochemical performance of prepared CB/AuNPs/GCE were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), Ultraviolet-visible spectroscopy (UV-vis), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and electrochemical technologies. The effects of pH, scanning rate, enrichment time, enrichment potential and the speculated mechanism of cannabidiol reaction at modified electrode were studied in detail. Under the optimized conditions, the fabricated sensing platform exhibited a great linear response of 0.42 mu A mu M-1 in the concentration range of 0.25 similar to 50.0 mu M. The prepared CB/AuNPs/GCE showed long-term stability, good repeatability and high practicability in real samples of industrial hemp. The proposed sensor can be further enhanced for portable and rapid detection of plant secondary metabolites, providing a basis for food detection, agricultural development, and drug detection.
作者机构：
[Chen, Zhiqun; Yan, Li; Feng, Guifang] Linyi Univ, Coll Life Sci, Linyi 276000, Peoples R China.;[Feng, Guifang] Philippine Christian Univ, Ctr Int Educ, Manila 1004, Philippines.;[Hao, Xiaodong; Zhu, Ping] Linyi Univ, Coll Resources & Environm, Linyi 276000, Peoples R China.;[Xiao, Yunhua] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Yunhua Xiao; Xiaodong Hao] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Resources and Environment, Linyi University, Linyi 276000, China
关键词：
Cd-contaminated soils;mixotrophic acidophiles;free and attached communities;microbial diversity;Cd removal
摘要：
In this study, cadmium (Cd)-contaminated soils were bioremediated using the mixotrophic acidophiles in different scale-up cultivation stages. The free and attached bacterial and fungal diversities and community structures, and their effects on soil Cd removal were investigated. Percentages of dominant bacteria (Acidithiobacillus, Sulfobacillus, and Pseudomonas) and fungi (Acidomyces and Coniochaeta) between the free and attached communities exhibited the pronounced differences. The alpha-diversity of attached bacteria was higher than the free bacteria, while the distinct variations in attached and free fungi were not observed. However, the beta-diversity between the free and attached bacteria and fungi was significantly changed. Solution pH and reduced glucose level affected the abundance patterns of Acidithiobacillus in free bacteria and Acidomyces in attached fungi, which had great contributions to the soil Cd removal efficiency.
通讯机构：
[Xia, M; Zhou, Z ] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Prov Engn Technol Res Ctr Opt Agr, Changsha 410128, Peoples R China.
关键词：
Phosphor;Luminescence;NIR emission;Bi3+
摘要：
Herein, the strategy of replacing Ge4+ with smaller Si4+ was adopted to realize the site-selective occupation of Bi3+ activator in the small ring and obtain a near-infrared light-emitting in Zn-2(Ge,Si)O-4. The designed phosphor exhibits a broad NIR emission with FWHM approximate to 104 nm in the 650-860 nm region, with a center emission wavelength of about 750 nm. Interestingly, the more sensitive four-member ring sites gradually replaced the six-member ring sites and realized a large-scope photoluminescence regulation from blue to NIR by just after the crystal field engineering. The possible reasons for this phenomenon can be interpreted by centroid shift (epsilon(c)) and crystal field splitting (epsilon(cfs)). This work not only provides new insights for the development of Bi3+-activated NIR-emitting phosphors, but also provides thoughts for revealing the potential NIR luminous mechanism of Bi3+.
