摘要：
The color of the seed coat has great diversity and is regarded as a biomarker of metabolic variations. Here we isolated a soybean variant (BLK) from a population of recombinant inbred lines with a black seed coat, while its sibling plants have yellow seed coats (YL). The BLK and YL plants showed no obvious differences in vegetative growth and seed weight. However, the BLK seeds had higher anthocyanins and flavonoids level and showed tolerance to various abiotic stresses including herbicide, oxidation, salt, and alkalinity during germination. Integrated metabolomic and transcriptomic analyses revealed that the upregulation of biosynthetic genes probably contributed to the overaccumulation of flavonoids in BLK seeds. The transient expression of those biosynthetic genes in soybean root hairs increased the levels of total flavonoids or anthocyanins. Our study revealed the molecular basis of flavonoid accumulation in soybean seeds, leveraging genetic engineering for both nutritious and stress-tolerant soybean germplasm.
关键词：
Spent mushroom compost;Nitrogen loss;Microbial community structure;Core microorganisms;Nitrogen metabolism;Chicken manure
摘要：
Due to the rapid development of animal husbandry, the associated environmental problems cannot be ignored, with the management of livestock and poultry manure emerging as the most prominent issue. Composting technology has been widely used in livestock and poultry manure management. A deeper understanding of the nitrogen conversion process during composting offers a theoretical foundation for selecting compost substrates. In this study, the effects of sawdust (CK) and spent mushroom compost (T1) as auxiliary materials on nitrogen as well as microbial structure in the composting process when composted with chicken manure were investigated. At the end of composting, the nitrogen loss of T1 was reduced by 17.18% relative to CK. When used as a compost substrate, spent mushroom compost accelerates the succession of microbial communities within the compost pile and alters the core microbial communities within the microbial community. Bacterial genera capable of cellulose degradation (Fibrobacter, Herbinix) are new core microorganisms that influence the assimilation of nitrate reduction during compost maturation. Using spent mushroom compost as a composting substrate increased the enzyme activity of nitrogen assimilation while decreasing the enzyme activity of the denitrification pathway.
通讯机构：
[Wangqiu Deng] S;State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute ofMicrobiology, Guangzhou 510070, China
会议名称：
19th World Congress of the International-Society-on-Toxinology (IST) / 11th Asia Pacific Congress of the International-Society-on-Toxinology (IST) / 13th Chinese Conference on Toxins and Medical Applications
会议时间：
OCT 24-31, 2017
会议地点：
Haikou, PEOPLES R CHINA
关键词：
Amanita;mushroom poisining;cyclopeptide;amanitin;phallacidin;gene family
通讯机构：
[Jinjun Chen] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R. China
会议名称：
19th World Congress of the International-Society-on-Toxinology (IST) / 11th Asia Pacific Congress of the International-Society-on-Toxinology (IST) / 13th Chinese Conference on Toxins and Medical Applications
通讯机构：
[Jinjun Chen] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China
会议名称：
19th World Congress of the International-Society-on-Toxinology (IST) / 11th Asia Pacific Congress of the International-Society-on-Toxinology (IST) / 13th Chinese Conference on Toxins and Medical Applications
摘要：
The effects of three surfactants (Tween 80, saponin and monorhamnolipid) on the hydrolysis of alkali pretreated rice straw by low dosage of cellulase were studied. The results indicated that with a relatively low cellulase dosage (4 FPU g(-1) substrate), all surfactants were able to enhance the enzymatic hydrolysis, while the biosurfactant monorhamnolipid was demonstrated to be more active than Tween 80 and saponin. Monorhamnolipid at 0.006% increased reducing sugar yield by 23.15%, and the maximal sugar yield was obtained within half of the time compare to the control required. The cellulase losed 67% of its activity in the control, while the activity lost ratios were 31%similar to 43% in the presence of the surfactants. The surfactants addition did not affect the pH of the hydrolysis system. This study provides an opportunity of decreasing enzyme dosage in hydrolysis of agricultural solid waste rich in cellulose.
摘要：
Scorpion venom is usually utilized to induce cancer cell death via apoptosis pathways. Our study focuses on the effects of the Heterometrus liangi scorpion venom on the proliferation of KYSE-510 cells (human esophageal cancer cells) and its related molecular mechanism. Marked morphological changes in KYSE-510 cells after treatment with scorpion venom were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell apoptosis were determined using flow cytometry (FCM). Moreover, RT-PCR and Western blot analysis was used to investigate the different levels of p21 and caspase-3 expression. Our results showed that scorpion venom at concentrations of 50-100 ug/mL caused dose-dependent inhibition of KYSE-510 cell proliferation. And, the ratio of apoptosis and necrosis increased. Besides, the effect of venom treated in KYSE-510 cells in p21 gene expression was reinforced, but the caspase-3 gene expression had indistinctive effect. The levels of p21 and caspase-3 protein expression were up-regulated after scorpion venom treatment. Therefore, scorpion venom may increase the expressions of p21 and caspase-3 protein to inhibite KYSE-510 proliferation and induce cell apoptosis selectively.
摘要：
Pig production plays an important role in farming systems worldwide, especially in subtropical areas. The past few decades have seen significant changes in swine production in such regions. However, there are regional differences in pig production, and some of these are associated with serious problems which impact production systems, the environment and human health. This review introduces the pig breeds, crops and challenge of pig production that faces subtropical areas. A detailed analysis focuses on the control of production problems that are affected by limitations in management and nutritional strategies. Then, factors that drive the major changes in the pig industry in this area are examined in detail, and some insight into pig production directions is provided. Copyright (c) 2011 Society of Chemical Industry
摘要：
Ancient pyrometallurgical zinc generated large amounts of slags. Natural leaching from ancient pyrometallurgical zinc sites has contaminated the soil closed to the deposits. Electrokinetic removal technology was adopted to treat this slags. But the generation of metal hydroxide precipitation near the cathode during the electrokinetic removal process could be the most serious factor that influenced the removal rate. In this study, cation exchange membrane was used in electrokinetic removal process to overcome this major drawbacks. The final results showed that cation exchange membrane could effectively condition pH of the whole electrokinetic removal system resulted in the dissolution of the precipitated heavy metals near the cathode. In other words, the enhanced treatment have achieved positive effect to some extent. But the leak tightness of experiment setup might not be perfect. Moreover，some operation parameters of the electrokinetic removal technique must be further optimized.
摘要：
A piezoelectric quartz crystal impedance technique has been applied for in situ monitoring of the whole process of the proteolytic enzyme-catalyzed hydrolyzation of casein and the effect of pesticide dimehypo (formula: C5H11NNa2O6S4) on the proteolytic enzyme activity in the midgut of the spider Pardosa pseudoannulata, based on its real-time reponses to the density-viscosity change of the tested solution due to the hydrolyzation occurring on the casein molecules. The results indicated that the hydrolyzation was significantly increased in the presence of low-dose dimehypo, where significantly inhibited in the presence of high-dose dimehypo, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of dimehypo, with a significant frequency response observed even at a 2.88 x 10-4 volume ratio of dimehypo to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose dimehypo pesticide and quick detection of proteolytic enzyme activity.
通讯机构：
[Zhang, Weiwei] S;Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China.
会议名称：
International Conference on Contaminated Sites Remediation 2011 International Forum (RCST 2011)
会议时间：
OCT 25-27, 2011
会议地点：
Environm Sci Soc Chongqing, Chongqing, PEOPLES R CHINA
会议主办单位：
Environm Sci Soc Chongqing
会议论文集名称：
Advanced Materials Research
关键词：
Bioleaching;Pyritic Mineral Processing Solid Waste;Thiobacillus Ferrooxidans;Thiobacillus Thiooxidans
摘要：
The produced and extraction process of pyritic would generate a large amount of pyritic mineral processing solid waste. This kind of waste can lead to land occupation, and contaminate the local water, air and soil. Therefore, in this paper, Thiobacillus ferrooxidans and Thiobacillus thiooxidans are used to deal with the pyritic mineral processing solid waste. The particles, catalysts and bioleaching products between microorganism and pyritic mineral processing solid wast were studied. It is indicated that the microorganism growth and the heavy metals leaching efficiency involve biological and chemical interactions. The experimental results suggested that the T.t was best to bioleach the pyritic mineral processing solid waste, and the efficiency of T.f was worse than that of T.t. The leaching effect of mixed bacterias of T.t and T.f fell in between, the leaching rate would decrease when the pulp density increased. The micro-morphology of bacteria was observed by SEM detector, the single cellular dimension of bioleaching bactreia was about (1 similar to 2) mu mx (0.2 similar to 0.5) mu m.
摘要：
This paper includes the analysis of total content of Cd element of 102 samples in the surface soil of farmland around the slag field. We evaluated the contamination of Cd of each samples and the space distribution with the potential ecological risk index method based on ArcGIS soft. The results showed that: average of potential ecological risk index of Cd is 241.4, Moderate ecological risk area accounted for 2.7% of the study area, strong ecological risk area of the region accounted for 30.1% of the study area, 22.2% of the study area had the considerable ecological risk, and the rest 39% of the study area had the extremely strong ecological risk. Mineral resources provides important energy to national economy and our daily life, but with the development and utilization of mineral resources significantly increasing, the harm to the environment of heavy metals in smelting wastes gets more and more attention. Cd is a kind of hazardous element, if we intake cadmium-containing substances for a long while, we may Suffer from "Itai-Itai Disease" and other diseases or even die. There are lots of international and domestic researches about the ecological hazards of heavy metals on environment in the surrounding area of slag field in recent years. Pazi I has evaluated heavy metals in surface sediments of Candarli Gulf with the potential ecological risk index<sup>[1]</sup>. Zhao Xinna has done the research of the ecological pollution in the part of soil in Shanghai<sup>[2]</sup>. Wu Di has evaluated the potential ecological risk in the sediment of Wujiang River<sup>[3]</sup>. This paper takes the Cd element in the farmland near slag field as example, and evaluates the pollution caused by Cd element with the potential ecological risk index.
摘要：
This paper focuses on the effect of the venom of the scorpion Heterometrus liangi on the proliferation of human KYSE-510 cell line and the related molecular mechanism. Marked morphological changes in
摘要：
This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela cell line and the related molecular mechanism. MTT test showed that
