作者:
Dan CHU;Bin CHEN;Bo WENG;Sai-na YAN;Yan-fei YIN;...
期刊:
农业科学学报(英文),2023年 ISSN:2095-3119
作者机构:
[Dan CHU; Bin CHEN; Sai-na YAN; Yan-fei YIN; Xiang-wei TANG; Mao-liang RAN] College of Animal Science and Technology, Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Hunan Agricultural University, Changsha 410128, P.R.China;Xiangxi Vocational and Technical College for Nationalities, Xiangxi 416007, P.R.China;[Bo WENG] College of Animal Science and Technology, Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Hunan Agricultural University, Changsha 410128, P.R.China<&wdkj&>Xiangxi Vocational and Technical College for Nationalities, Xiangxi 416007, P.R.China
摘要:
Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility. Although a huge number of long non-coding RNAs (lncRNAs) are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis, their functions and regulatory mechanisms are still in infancy. Herein, we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells. The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells. lncFPFSC overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis, whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects. Mechanistically, lncFPFSC acted as a sponge for miR-326. Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis, which further abolished the effects of lncFPFSC overexpression. The euchromatic histone-lysine N-methyltransferase 2 (EHMT2) gene was directly targeted by miR-326, and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells. Then, siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition. Collectively, lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis. This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells, and the competing endogenous RNA (ceRNA) network provided new molecular markers to treat Sertoli cell disorder induced male infertility.
通讯机构:
[Yulong Yin; Jiashun Chen] A;Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China<&wdkj&>CAS Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Changsha, Hunan, 410125, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China<&wdkj&>CAS Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Changsha, Hunan, 410125, China
摘要:
This paper was to determine the effects of dietary Litsea cubeba essential oil (LEO) supplementation on growth performance, immune function, antioxidant level, intestinal morphology and microbial composition in weaned piglets. One hundred and ninety-two piglets (Duroc x [Large White x Landrace]) with 6.85 +/- 0.22 kg mean body weight weaned at 21 d of age were randomly assigned to 4 treatment groups with 8 replicates and were fed with a basal diet (CON) or CON diet containing 100 (LLEO), 200 (MLEO) and 400 (HLEO) mg/kg LEO. The results revealed that HLEO supplementation (P < 0.05) increased the average daily gain on d 28 compared with CON. MLEO and HLEO supplementation decreased (P < 0.05) feed conversion ratio. LEO-containing diets had a lower (P < 0.05) diarrhea rate. Supple-mentation with HLEO increased (P < 0.05) total antioxidant capacity (T-AOC) both in the serum and liver. Meanwhile, the supplementation of MLEO and HLEO resulted in higher (P < 0.05) glutathione peroxidase (GPx) activities both in serum and liver. Supplementation of HLEO increased (P < 0.05) serum immu-noglobulin A, immunoglobulin G and interleukin-10, whereas supplementation with MLEO and HLEO decreased (P < 0.05) tumor necrosis factor-a. Villus height in the duodenum or jejunum was increased (P < 0.05) in the HLEO group, and the villus height to crypt depth ratio in the jejunum was also improved (P < 0.05) in the MLEO group. The addition of LEO increased (P < 0.05) the richness and diversity of the microbial community in the cecum, which mainly increased the relative abundance of Oscillospir-aceae_UCG-005, Faecalibacterium, Blautia and Coprococcus. Piglets supplemented with HLEO increased (P < 0.05) the concentration of short chain fatty acids (SCFA), including acetic acid in the cecum and propionic acid in the colon. In conclusion, these findings indicated that LEO supplementation improved growth performance and intestinal health in weaned piglets. (c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
摘要:
Abstract: MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial regulatory roles in many biological processes, including the growth and development of skeletal muscle. miRNA-100-5p is often associated with tumor cell proliferation and migration. This study aimed to uncover the regulatory mechanism of miRNA-100-5p in myogenesis. In our study, we found that the miRNA-100-5p expression level was significantly higher in muscle tissue than in other tissues in pigs. Functionally, this study shows that miR-100-5p overexpression significantly promotes the proliferation and inhibits the differentiation of C2C12 myoblasts, whereas miR-100-5p inhibition results in the opposite effects. Bioinformatic analysis predicted that Trib2 has potential binding sites for miR-100-5p at the 3′UTR region. A dual-luciferase assay, qRT-qPCR, and Western blot confirmed that Trib2 is a target gene of miR-100-5p. We further explored the function of Trib2 in myogenesis and found that Trib2 knockdown markedly facilitated proliferation but suppressed the differentiation of C2C12 myoblasts, which is contrary to the effects of miR-100-5p. In addition, co-transfection experiments demonstrated that Trib2 knockdown could attenuate the effects of miR-100-5p inhibition on C2C12 myoblasts differentiation. In terms of the molecular mechanism, miR-100-5p suppressed C2C12 myoblasts differentiation by inactivating the mTOR/S6K signaling pathway. Taken together, our study results indicate that miR-100-5p regulates skeletal muscle myogenesis through the Trib2/mTOR/S6K signaling pathway. Keywords: miR-100-5p; proliferation; differentiation; Trib2; mTOR; C2C12 myoblast
作者机构:
[Yin, Jie; Li, Yunxia; Tian, Yu; Zhou, Feng; Ma, Jie; Xia, Siting; Yang, Tong; Liu, Gang; Huang, Xingguo] College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China;[Ma, Libao] College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China;Hunan Chuweixiang Agriculture and Animal Husbandry Co., Ltd., Ningxiang 410600, China;Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;[Zeng, Qinghua] College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China<&wdkj&>Hunan Chuweixiang Agriculture and Animal Husbandry Co., Ltd., Ningxiang 410600, China
通讯机构:
[Yulong Yin; Xingguo Huang] C;College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China<&wdkj&>College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China<&wdkj&>Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
摘要:
The gut microbiota consistently shows strong correlations with lipid metabolism in humans and animals, and whether the gut microbiota contributes to muscle fatty acid (FA) deposition and meat traits in farm animals has not been fully resolved. In this study, we aimed to unveil the microbial mechanisms underlying muscle FA deposition in pigs. First, we systematically revealed the correlation between the gut microbiome and muscle FA levels in 43 obese Ningxiang pigs and 50 lean Duroc Landrace Yorkshire (DLY) pigs. Mutual fecal microbial transplantation showed that the obese Ningxiang pig-derived microbiota increased the muscle FA content and improved meat quality by reshaping the gut microbial composition in lean DLY pigs. Lactobacillus reuteri has been identified as a potential microbial biomarker in obese Ningxiang pig-derived microbiota-challenged DLY pigs. A gavage experiment using lean DLY pigs confirmed that L. reuteri XL0930 isolated from obese Ningxiang pigs was the causal species that increased the muscle FA content. Mechanistically, SLC22A5, a carnitine transporter, was downregulated in L. reuteri XL0930-fed DLY pigs, resulting in reduced muscle carnitine levels. Muscle and intestinal L-carnitine levels, which correlated with the muscle FA content, impeded fat synthesis and FA accumulation in invitro and invivo models. In conclusion, we uncovered an unexpected and important role of the obese Ningxiang pig-derived microbiota in regulating muscle FA metabolism via the SLC22A5-mediated carnitine system.
摘要:
The purpose of this experiment was to use a single-factor design to investigate the effects of different supplementation levels of plant essential oil/palygorskite composite (EPO-Pal) on growth performance, blood parameters, and intestinal morphology of broiler chicken. A total of 960 one-day-old chicks were randomly assigned into 4 treatments with 6 replicates of 40 chicks each. The broilers were fed the basal diet supplemented with 0, 500, 750, and 1000 mg/kg EPO-Pal for 42 days, respectively. The results showed that the 500 or 750 mg/kg groups significantly increased the body weight, average daily feed intake and average daily gain of the broilers at 28 and 42 days (p < 0.05). Additionally, as compared to the control group, the 1000 mg/kg EPO-Pal group exhibited higher levels of immunoglobulins-A, immunoglobulins-G, immunoglobulins-M, and high-density lipoprotein cholesterol while having lower levels of tumour necrosis factor-alpha (p < 0.05). The content of interleukin-2 and the villus height-to-crypt depth ratio were significantly increased in the 750 mg/kg EPO-Pal group (p < 0.05). Moreover, the activity of total antioxidant capacity and superoxide dismutase were significantly enhanced in the 500 mg/kg EPO-Pal group (p < 0.05). The results are very promising, and EPO-Pal can be considered as an additive to promote the growth by enhancing immunity, antioxidant activity, and improving the intestinal morphology of broiler chickens.
摘要:
Myxobolus zhaltsanovae n. sp., is described from the gills of gibel carp Carassius gibelio found during a survey of myxozoans from the watershed of Lake Baikal, Russia, based on morphological and molecular characterizations. Plasmodia of M. zhaltsanovae n. sp. develop extravascularly and measure 500-1000 mu m long, 25-100 mu m wide. The myxospore is circular to oval, measuring 13.23 +/- 0.09 (11.3-14.8) mu m (mean +/- SD, range) in length, 10.19 +/- 0.07 (9.1-11.4) mu m in width, and 6.49 +/- 0.12 (5.4-7.2) mu m in thickness. Polar capsules are unequal and subspherical; measurements of polar capsules are: length 5.62 +/- 0.06 (4.7-6.7), width 3.44 +/- 0.04 (2.4-4.4) mu m and length 3.42 +/- 0.05 (2.5-4.1), width 1.94 +/- 0.04 (1.3-3.3) mu m. Phylogenetic analysis with the 18S rDNA gene shows M. zhaltsanovae n. sp. as a sister species of the subclade formed by M. musseliusae, M. tsangwuensis, and M. basilamellaris, which infect common carp Cyprinus carpio.
摘要:
Abstract This experiment was conducted to investigate the effects of magnolol on the oxidative parameters and jejunum injury induced by diquat in broiler chickens. This test adopts a 2 × 2 factors design, a total of 288 one‐day‐old male AA broiler chicks randomly allocated to four groups, consisting of six replicates of 12 birds each, which was then denoted as CON group, diquat (DIQ) group (16 mg/kg BW diquat was injected into birds at the age of 21 days), magnolol (MAG) group (basic bird diet supplemented with 300 mg/kg magnolol), and MAG + DIQ group. At 21 days of age, broilers in the DIQ group and the MAG + DIQ group were intraperitoneally injected with 16 mg/kg BW diquat. Results showed that diet supplementing with MAG could alleviate the decrease of ADG to a certain extent after exposure to DIQ. Addition of magnolol to the diet alleviated the decrease of ADG during injection, antioxidant enzymes, and gene expression and increased the markers of oxidative damage induced by diquat induction. Magnolol supplement reversed the increase of apoptotic cells in the diquat‐induced chicken jejunum. RNA sequencing showed that PI3K–Akt, calcium, and NF‐kappa B signaling pathways were the main enrichment pathways between the DIQ group and the MAG + DIQ group. Our findings revealed that magnolol may improve antioxidant enzyme activity and expression of related genes through the PI3K–Akt pathway to alleviate oxidative stress.
摘要:
Corrigendum: The Amino Acids Sensing and Utilization in Response to Dietary Aromatic Amino Acid Supplementation in LPS-Induced Inflammation Piglet ModelAffiliations of all authors as they appear in the published original version of the article (INSERT ONLY IF correcting affiliation(s) or adding affiliation(s). Insert the correct version of the affiliation(s))Qing Duanmu1 , Bie Tan1,2 *, Jing Wang1 , Bo Huang1,2, Jianjun Li 2 , Meng Kang1 , Ke Huang1 , Qiuchun Deng1 and Yulong Yin1,2 * Correspondence: bietan@hunau.edu.cnKeywords: same as original articleCorrigendum on: Qing Duanmu1 , Bie Tan2 *, Jing Wang1 , Bo Huang1,2, Jianjun Li 2 , Meng Kang1 , Ke Huang1 , Qiuchun Deng1 and Yulong Yin1,2 Additional Affiliation(s)In the published article, there was an error regarding the affiliation(s) for [Bie Tan]. As well as having affiliation(s) [2], they should also have [College of Animal Science and Technology, Hunan Agricultural University, Changsha, China,].The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.