作者机构：
湖南农业大学动物科学技术学院,湖南畜禽安全生产协同创新中心,长沙410128;中国科学院亚热带农业生态研究所,中国科学院亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,动物营养生理与代谢过程湖南省重点实验室,长沙410125;[蒋线吉; 印遇龙] Hunan Co⁃Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, 410128, China, Key Laboratory of Animal Nutritional Physiology and Metabolic Process of Hunan, National Engineering Laboratory for Poultry Breeding Pollution Control and Resource Technology, Key Laboratory of Agro⁃Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China;[李瑞; 冯淦熠] Key Laboratory of Animal Nutritional Physiology and Metabolic Process of Hunan, National Engineering Laboratory for Poultry Breeding Pollution Control and Resource Technology, Key Laboratory of Agro⁃Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China
通讯机构：
[Li, R.] K;Key Laboratory of Animal Nutritional Physiology and Metabolic Process of Hunan, China
关键词：
iron homeostasis;mitochondrial dysfunction;diseases
摘要：
Iron is a trace element necessary for cell growth, development, and cellular homeostasis, but insufficient or excessive level of iron is toxic. Intracellularly, sufficient amounts of iron are required for mitochondria (the center of iron utilization) to maintain their normal physiologic function. Iron deficiency impairs mitochondrial metabolism and respiratory activity, while mitochondrial iron overload promotes ROS production during mitochondrial electron transport, thus promoting potential disease development. This review provides an overview of iron homeostasis, mitochondrial iron metabolism, and how mitochondrial iron imbalances-induced mitochondrial dysfunction contribute to diseases.
摘要：
The purpose of this experiment was to investigate the effects of Litsea cubeba essential oil (LCO) on the growth performance, blood antioxidation, immune function, apparent digestibility of nutrients, and fecal microflora in fattening pigs. A total of 120 pigs were randomly assigned to five groups, with six replicate pens per treatment and four pigs per pen, and they were fed basal diet, chlortetracycline (CTC), and low-, medium-, and high-concentration LCO. The results of the study showed that compared with the control treatment and CTC addition treatment of the basic diet, the catalase level in the serum of the pigs treated with 500 mg/kg LCO in the diet of finishing pigs was significantly increased (p < 0.05). The apparent digestibility of crude protein, crude ash, and calcium in pigs with different levels of LCO was significantly increased compared with the control treatments fed the basal diet (p < 0.05). In addition, compared with the control treatment fed the basal diet and the treatment with CTC, the apparent digestibility of ether extract in pigs treated with medium-dose LCO was significantly increased (p < 0.05), and the apparent digestibility of pigs was significantly increased after the addition of low-dose LCO (p < 0.05). Among the genera, the percentage abundance of SMB53 (p < 0.05) was decreased in the feces of the CTC group when compared to that in the medium-LCO group. At the same time, the relative abundance of L7A_E11 was markedly decreased in the feces of the control and medium- and high-concentration LCO group than that in the CTC group (p < 0.05). In conclusion, adding the level of 250 mg/kg LCO in the diet of pig could improve the growth performance and blood physiological and biochemical indicators of pigs, improve the antioxidant level of body and the efficiency of digestion and absorption of nutrients, and show the potential to replace CTC.
摘要：
The purpose of this study was to evaluate the effects of limiting nutrients and the N: P ratios on the growth of phytoplankton. In this study, we used the nutrient enrichment bioassays (NEB), Multivariate analysis of variance, and regression analysis. The results showed that the affected change of phytoplankton assemblages growth was the concentrations of nitrogen and phosphorous. During the same period, the response time of phytoplankton to the input of external nutrients was similar. The growth rates of phytoplankton were not persistent. This is related to the differences in environmental conditions, dominant species composition, and phytoplankton growth strategies. Different dominant species also had different response mechanisms to nutrient addition. The growth effect of phytoplankton in the preliminary experiment in the laboratory was more significant than that in the field experiment. Water temperature and light were also important environmental factors affecting the growth of phytoplankton. Multivariate analysis of variance showed that phosphorus addition alone had a more significant stimulation on phytoplankton growth than that of nitrogen, especially for Synedra. But addition of both nitrogen and phosphorus had the most stimulating effect on Cyanophyta and Chlorophyta. The phytoplankton had the maximum growth in TN: TP = 19.87, phytoplankton growth was promoted with 0.9 & SIM; 13.59 mg/L TN and 0.02 & SIM; 0.684 mg/L TP. When the total phosphorus in summer was greater than 0.04 mg/L, the possibility of algal bloom and will be greater. The linear regression fitted chlorophyll a and phosphorus well, and the regression equation was as follows: Log(10)(Chl.a) = 2.154 + 0.5103 x Log(10)(TP). The binary regression equation of chlorophyll a and nitrogen and phosphorus was as follows: Chl.a = 33.84 + 1.769TN + 123.9TP.
作者：
Pu, Xuan Xuan;Zhang, Xiu Min;Li, Qiu Shuang;Wang, Rong;Zhang, Min;...
期刊：
Frontiers in Microbiology,2023年13:1079056 ISSN：1664-302X
通讯作者：
Tan, Z.L.;Wang, M.
作者机构：
[Tan, Zhi Liang; Tan, Bie; Pu, Xuan Xuan] Univ Hunan Agr Univ, Dept Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Tan, Zhi Liang; Zhang, Xiu Min; Wang, Rong; Pu, Xuan Xuan; Zhang, Shi Zhe; Li, Qiu Shuang; Wang, Min] Chinese Acad Sci, Inst Subtrop Agr, CAS Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Live, Changsha, Hunan, Peoples R China.;[Lin, Bo; Zhang, Min] Univ Guangxi, Dept Anim Sci & Technol, Nanning, Guangxi, Peoples R China.
通讯机构：
[Tan, Z.L.] D;[Wang, M.] C;CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan, China;Department of Animal Science and Technology, Hunan, China
通讯机构：
[Yulong Yin; Jiashun Chen] A;Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China<&wdkj&>CAS Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Changsha, Hunan, 410125, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China<&wdkj&>CAS Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Changsha, Hunan, 410125, China
摘要：
This paper was to determine the effects of dietary Litsea cubeba essential oil (LEO) supplementation on growth performance, immune function, antioxidant level, intestinal morphology and microbial composition in weaned piglets. One hundred and ninety-two piglets (Duroc x [Large White x Landrace]) with 6.85 +/- 0.22 kg mean body weight weaned at 21 d of age were randomly assigned to 4 treatment groups with 8 replicates and were fed with a basal diet (CON) or CON diet containing 100 (LLEO), 200 (MLEO) and 400 (HLEO) mg/kg LEO. The results revealed that HLEO supplementation (P < 0.05) increased the average daily gain on d 28 compared with CON. MLEO and HLEO supplementation decreased (P < 0.05) feed conversion ratio. LEO-containing diets had a lower (P < 0.05) diarrhea rate. Supple-mentation with HLEO increased (P < 0.05) total antioxidant capacity (T-AOC) both in the serum and liver. Meanwhile, the supplementation of MLEO and HLEO resulted in higher (P < 0.05) glutathione peroxidase (GPx) activities both in serum and liver. Supplementation of HLEO increased (P < 0.05) serum immu-noglobulin A, immunoglobulin G and interleukin-10, whereas supplementation with MLEO and HLEO decreased (P < 0.05) tumor necrosis factor-a. Villus height in the duodenum or jejunum was increased (P < 0.05) in the HLEO group, and the villus height to crypt depth ratio in the jejunum was also improved (P < 0.05) in the MLEO group. The addition of LEO increased (P < 0.05) the richness and diversity of the microbial community in the cecum, which mainly increased the relative abundance of Oscillospir-aceae_UCG-005, Faecalibacterium, Blautia and Coprococcus. Piglets supplemented with HLEO increased (P < 0.05) the concentration of short chain fatty acids (SCFA), including acetic acid in the cecum and propionic acid in the colon. In conclusion, these findings indicated that LEO supplementation improved growth performance and intestinal health in weaned piglets. (c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
通讯机构：
[Dingfu Xiao; Xiaokang Ma] A;Authors to whom correspondence should be addressed.<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
期刊：
FRONTIERS IN IMMUNOLOGY,2023年14:1320443 ISSN：1664-3224
作者机构：
State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, China;College of Animal Science and Technology, Hunan Agricultural University, China;Department of Pulmonary and Critical Care Medicine Third Affiliated Hospital of Sun Yat-sen University, China;Faculty of Biosciences and Aquaculture, Nord University, Norway
摘要：
Immune cells undergo changes during infection, serving as defense cells, conducting microbial surveillance, and maintaining homeostasis. These processes are supported by metabolic regulations that provide the necessary energy and nutrients. Immune processes and metabolic regulation are highly integrated, but information on their interdependency is limited, which is a key discussion in this research topic. This editorial's summary reveals a diverse collection of articles, covering topics such as nutritional regulation of immune responses, the gut microbiota-immune axis, virulence reduction using metabolites, development of attenuated live vaccines through the deletion of bacterial transcription factor genes, host reprogramming using metabolites as a protective measure against microbial infections, and the identification of endogenous compounds in host cells with potential as drug candidates against microbial infections.Hu et al reviewed the interaction between dietary fiber (DF) and gut microbiota in pigs. They underscored the pivotal role of DF in microbial fermentation, generating short-chain fatty acids that serve as the primary energy source. Additionally, DF plays a vital role in upholding normal intestinal function, homeostasis, and immunomodulation, thus mitigating infection and inflammation. Their assertions were substantiated by referencing pertinent studies [1,2] fructose with a live Edwasiella tarda vaccine significantly increased the survival rates of vaccinate...
作者机构：
[Shan, Yanke; Lu, Yu-Nan; Yi, Weijie; Wang, Bin; Li, Jiahao; Guo, Jiajing; Wang, Shouyu; Liu, Fei] Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Jiangsu, Nanjing;210095, China;[Li, Wenzhi] Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, Xi'an;710000, China;College of Animal Science and Technology, Hunan Agricultural University, Hunan, Changsha, China
通讯机构：
[Yulong Yin] C;[Fei Liu] J;College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China<&wdkj&>Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China
摘要：
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial regulatory roles in many biological processes, including the growth and development of skeletal muscle. miRNA-100-5p is often associated with tumor cell proliferation and migration. This study aimed to uncover the regulatory mechanism of miRNA-100-5p in myogenesis. In our study, we found that the miRNA-100-5p expression level was significantly higher in muscle tissue than in other tissues in pigs. Functionally, this study shows that miR-100-5p overexpression significantly promotes the proliferation and inhibits the differentiation of C2C12 myoblasts, whereas miR-100-5p inhibition results in the opposite effects. Bioinformatic analysis predicted that Trib2 has potential binding sites for miR-100-5p at the 3'UTR region. A dual-luciferase assay, qRT-qPCR, and Western blot confirmed that Trib2 is a target gene of miR-100-5p. We further explored the function of Trib2 in myogenesis and found that Trib2 knockdown markedly facilitated proliferation but suppressed the differentiation of C2C12 myoblasts, which is contrary to the effects of miR-100-5p. In addition, co-transfection experiments demonstrated that Trib2 knockdown could attenuate the effects of miR-100-5p inhibition on C2C12 myoblasts differentiation. In terms of the molecular mechanism, miR-100-5p suppressed C2C12 myoblasts differentiation by inactivating the mTOR/S6K signaling pathway. Taken together, our study results indicate that miR-100-5p regulates skeletal muscle myogenesis through the Trib2/mTOR/S6K signaling pathway.
作者：
Dan CHU;Bin CHEN;Bo WENG;Sai-na YAN;Yan-fei YIN;...
期刊：
农业科学学报(英文),2023年 ISSN：2095-3119
作者机构：
[Dan CHU; Bin CHEN; Sai-na YAN; Yan-fei YIN; Xiang-wei TANG; Mao-liang RAN] College of Animal Science and Technology, Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Hunan Agricultural University, Changsha 410128, P.R.China;Xiangxi Vocational and Technical College for Nationalities, Xiangxi 416007, P.R.China;[Bo WENG] College of Animal Science and Technology, Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Hunan Agricultural University, Changsha 410128, P.R.China<&wdkj&>Xiangxi Vocational and Technical College for Nationalities, Xiangxi 416007, P.R.China
摘要：
Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility. Although a huge number of long non-coding RNAs (lncRNAs) are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis, their functions and regulatory mechanisms are still in infancy. Herein, we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells. The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells. lncFPFSC overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis, whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects. Mechanistically, lncFPFSC acted as a sponge for miR-326. Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis, which further abolished the effects of lncFPFSC overexpression. The euchromatic histone-lysine N-methyltransferase 2 (EHMT2) gene was directly targeted by miR-326, and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells. Then, siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition. Collectively, lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis. This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells, and the competing endogenous RNA (ceRNA) network provided new molecular markers to treat Sertoli cell disorder induced male infertility.
