摘要:
Graphical Abstract and Lay Summary Members of the GRAS gene family play important roles in regulating plant growth and development. In this study, the authors analyzed the GRAS family by bioinformatics and experimentally authenticated a member of this gene family (GhGRAS55) by using the VIGS technology, which demonstrated that the GhGRAS55 gene may have the function of regulating early maturation in cotton. This work has positively contributed to the in‐depth understanding of the molecular mechanisms regulating early maturing in cotton. Abstract The members of the GRAS gene family play important roles in regulating plant growth and development, but their functions in regulating early plant maturity traits are still unknown. In this study, we used a series of bioinformatics tools to identify GRAS gene family members and investigate the function of the gene family (GhGRAS55) using a genome‐wide database of upland cotton samples. A total of 58 members of the GRAS gene family were identified and screened, which were distributed on 21 chromosomes within the whole cotton genome. The results of the phylogenetic analysis showed that the genes of upland cotton, island cotton, African cotton, Raymond cotton, and Arabidopsis were distributed in subfamilies I–VIII, although subfamily II did not contain any upland cotton or Arabidopsis GRAS family members. The structures and other characteristics of the genes in this family were clarified using bioinformatics technology. The transcriptomic sequencing results for early and late maturing cotton species showed that the expression of most GRAS family genes, such as GhGRAS10, GhGRAS5511, and GhGRAS55, was lower in early maturing species than late maturing species. We also found that cotton plants with GhGRAS55 genes that were silenced by virus‐induced gene silencing (VIGS) technology showed early bud emergence phenotypes, so it could be speculated that the GhGRAS55 gene has the function of regulating early maturity in cotton.
通讯机构:
[Wang, YH ] Z;[Zhang, NL ] B;Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China.;Beijing Forestry Univ, State Key Lab Efficient Prod Forest Resources, Beijing 100083, Peoples R China.;Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing 100083, Peoples R China.
关键词:
salt spray;arbuscular mycorrhizal fungi;root trait;salinity tolerance;mycorrhizal colonization
摘要:
Salt spray is a major environmental issue in coastal areas. Cinnamomum camphora is an economically important tree species that grows in the coastal areas of southern China. Arbuscular mycorrhizal fungi (AMF) can alleviate the detrimental effects of abiotic stress on host plants. However, the mechanism by which AMF mitigates the adverse effects of salt spray on C. camphora remains unclear. A pot experiment was conducted in a greenhouse, where C. camphora seedlings were exposed to four AMF regimes (inoculation with sterilized fungi, with Glomus tortuosum, Funneliformis mosseae, either alone or in combination) and three salt spray regimes (applied with distilled water, 7, and 14 mg NaCl cm(-2)) in order to investigate the influence on root functional traits and plant growth. The results showed that higher salt spray significantly decreased the K+ uptake, K+/Na+ ratio, N/P ratio, total dry weight, and salinity tolerance of non-mycorrhizal plants by 37.9%, 71%, 27.4%, 12.7%, and 221.3%, respectively, when compared with control plants grown under non-salinity conditions. Mycorrhizal inoculation, particularly with a combination of G. tortuosum and F. mosseae, greatly improved the P uptake, total dry weight, and salinity tolerance of plants grown under higher salt spray conditions by 51.0%, 36.7%, and 130.9%, respectively, when compared with their counterparts. The results show that AMF can alleviate the detrimental effects of salt spray on C. camphora seedlings. Moreover, an enhanced uptake of K+ and P accounted for the resistance of the plants to salt spray. Therefore, pre-inoculation with a combination of G. tortuosum and F. mosseae to improve nutrient acquisition is a potential method of protecting C. camphora plants against salt spray stress in coastal areas.
作者机构:
[Zongyan Li] School of Biological Science and Technology, Hunan Agricultural University, Changsha, Hunan, China;[Huahai Chen; Aijie Hou; Yeshi Yin] Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, Hunan, China
通讯机构:
[Yeshi Yin] K;Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, Hunan, China
关键词:
Salmonella pullorum;Sanguinarine
摘要:
This work was performed on commercially purchased Salmonella pullorum CVCC519 originally isolated from chicken intestinal content. The Sanguinarine-resistant strain XM3104 was isolated from Sanguinarine-induced CVCC519. To identify possible mechanisms underlying resistance, the complete genomes of CVCC519 and XM3104 were sequenced using PromethION and next-generation sequencing.
摘要:
Lily Fusarium wilt disease caused by Fusarium spp. spreads rapidly and is highly destructive, leading to a severe reduction in yield. In this study, lily (Lilium brownii var. viridulum) bulbs were irrigated after planting with suspensions of two Bacillus strains that effectively control lily Fusarium wilt disease to assess their effects on the rhizosphere soil properties and microbial community. A high-throughput sequencing of microorganisms in the rhizosphere soil was performed and the soil physicochemical properties were measured. The FunGuild and Tax4Fun tools were used for a functional profile prediction. The results showed that Bacillus amyloliquefaciens BF1 and B. subtilis Y37 controlled lily Fusarium wilt disease with control efficacies of 58.74% and 68.93%, respectively, and effectively colonized the rhizosphere soil. BF1 and Y37 increased the bacterial diversity and richness of the rhizosphere soil and improved the physicochemical properties of the soil, thereby favoring the proliferation of beneficial microbes. The relative abundance of beneficial bacteria was increased and that of pathogenic bacteria was decreased. Bacillus abundance in the rhizosphere was positively correlated with most soil physicochemical properties, whereas Fusarium abundance was negatively correlated with most physicochemical properties. Functional prediction revealed that irrigation with BF1 and Y37 significantly upregulated glycolysis/gluconeogenesis among metabolism and absorption pathways. This study provides insights into the mechanism by which two Bacillus strains with antifungal activity, BF1 and Y37, antagonize plant pathogenic fungi and lays the foundation for their effective application as biocontrol agents.
摘要:
The complete mitochondrial genome of Brachyrhynchus triangulus Bergroth, 1889 was sequenced and annotated in the present study. It was a typical circular DNA molecule of 15,170 bp, with 37 genes and a control region. The percentages of A, C, G, and T nucleotides in the genome of B. triangulus were 41.1%, 17.4%, 11.9%, and 29.4%, respectively. Thirteen protein-coding genes (PCGs) start with a ATN codon or a TTG codon and terminate with a TAA codon or a TAG codon or a single T residue. With the exception of tRNA(Ser(AGN)) , each of the 22 tRNA genes had a clover-leaf structure and ranged in length from 62 to 69 bp. The length of lrRNA and srRNA was 1241 bp and 828 bp, respectively. The control region had a length of 708 bp and an A + T content of 74.6%. The sister relationship between B. triangulus and Brachyrhynchus hsiaoi is supported by the phylogenetic tree. Additionally, it proved the sister relationship between Mezirinae and Aneurinae, supporting the classical taxonomy of the Aradidae.
期刊:
Journal of Hazardous Materials,2023年454:131535 ISSN:0304-3894
通讯作者:
Zou, Q;Zou, Qi;Wu, C
作者机构:
[Pan, Wei-song; Xiong, Xiao-ran] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Zou, Qi] MEE, South China Inst Environm Sci, Guangzhou 510535, Peoples R China.;[Wu, Chuan; Wu, C; Qi, Yan-ting; Zou, Qi] Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.;[Hu, Min] Guangdong Acad Sci, Inst Eco Environm & Soil Sci, Guangdong Key Lab Integrated Agro Environm Pollut, Guangzhou 510520, Peoples R China.;[Li, Wai-chin] Educ Univ Hong Kong, Dept Sci & Environm Studies, Hong Kong, Peoples R China.
通讯机构:
[Zou, Q ] M;[Wu, C ; Zou, Q] C;MEE, South China Inst Environm Sci, Guangzhou 510535, Peoples R China.;Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.
摘要:
In the current study, a typical Sb mine was selected to explore the microbial community composition and assembly driven by the cocontamination of As/Sb with geographic distance. Our results showed that environmental parameters, especially pH, TOC, nitrate, total and bioavailable As/Sb contents largely affected the microbial community diversity and composition. The total and bioavailable As/Sb levels were significantly positively correlated with the relative abundance of Zavarzinella, Thermosporothrix and Holophaga, while the pH presented a significant negative correlation with the three genera, potentially implying they are important taxonomic groups in acid-mining soils. The cooccurrence network analysis indicated the environmental stress dominated by pH and As/Sb co-contamination affected the microbial modularity and interaction. Meanwhile, Homogeneous selection (HoS, 26.4-49.3%), and drift and others (DR, 27.1∼40.2%) were the most important assembly processes for soil bacterial, and the importance of HoS decreased and the DR increased with geographic distance to the contamination source respectively. Soil pH, nutrient availability, total and bioavailable As/Sb contents significantly affected the HoS and DR processes. This study provides theoretical support for microbial remediation in metal(loid)-contaminated soils.