摘要：
It has been reported that Arabidopsis chloroplast accD transcripts undergo RNA editing and that loss of accD-C794 RNA editing does not affect plant growth under normal conditions. To date, the exact biological role of accD-C794 editing has remained elusive. Here, we reveal an unexpected role for accD-C794 editing in response to heat stress. Loss of accD-C794 editing results in a yellow and dwarf phenotype with decreased chloroplast gene expression under heat stress, and artificial improvement of C794-edited accD gene expression enhances heat tolerance in Arabidopsis. These data suggest that accD-C794 editing confers heat tolerance in planta. We also found that treatment with the product of acetyl coenzyme A carboxylase (ACCase) could allay mutant phenotypic characteristics and showed that a mutation in theCAC3 gene for the α-subunit of ACCase was associated with dwarfism under heat stress. These observations indicate that defective accD-C794 editing may be intrinsic to reduced ACCase activity, thereby contributing to heat sensitivity. ACCase catalyzes the committed step of de novo fatty acid (FA) biosynthesis. FA content analysis revealed that unsaturated oleic (C18:1) and linoleic acids (C18:2) were low in the accD-C794 editing-defective mutant but high in the C794-edited accD-overexpressing plants compared with the wild type. Supplying exogenous C18:1 and C18:2 could rescue the mutant phenotype, suggesting that these FAs play an essential role in tolerance to heat stress. Transmission electron microscopy observations showed that heat stress seriously affected the membrane architecture in accD editing-defective mutants but not in accD-overexpressing plants. These results provide the first evidence that accD-C794 editing regulates FA biosynthesis for maintenance of membrane structural homeostasis under heat stress.
关键词：
gut microbiota;Lactiplantibacillus plantarum;faecal microbiota transplantation;serum metabolites
摘要：
Gut microbiota may have therapeutic effects on inflammatory bowel disease (IBD). Regulating intestinal microbiota through Lactiplantibacillus plantarum (L. plantarum) and faecal microbiota transplantation (FMT) is a novel approach to treating IBD. This study aimed to explore the effect of L. plantarum and FMT pretreatment in alleviating colitis in mice. Five groups of mice (n = 6 per group) were included: CON group, DSS group (dextran sodium sulphate-induced colitis mice), LP-DSS pretreatment group (colitis mice were given strain L. plantarum and 5% DSS), DSS-FMT group (mice pretreated with faecal microbiota transplantation were given 5% DSS), and LP-FMT pretreatment group (mice pretreated with faecal microbiota transplantation and L. plantarum were given 5% DSS). Serum metabolites and intestinal microbiota were analysed by 16S rRNA sequencing liquid chromatography-mass spectrometry (LC-MS). The results demonstrated that L. plantarum and FMT improved gut microbiota in mice by increasing Firmicutes and decreasing the Bacteroidetes. In the serum metabolomics analysis, there were 11 differential metabolites in the DSS-FMT and LP-FMT pretreatment groups, and these differential metabolites were mainly glycerophospholipids and sphingolipids. It is worth noting that Lachnospira and Lactobacillus were positively associated with 8 differential metabolites. These results suggest that L. plantarum and FMT can regulate intestinal microorganisms and serum metabolomics to alleviate inflammation.
通讯机构：
[Jun Zhang] K;[Yanxiu Zhao] A;Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Life Science College, Shandong Normal University, Jinan 250358, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Life Science College, Shandong Normal University, Jinan 250358, China
摘要：
ATP-binding cassette transporter G (ABCG) has been shown to be engaged in export of broad-spectrum compounds with structural differences, but little is known concerning its role in cutin formation of cotton (Gossypium spp.). In this study, we conduct a genome-wide survey and detected 69, 71, 124 and 131 ABCG genes within G. arboretum, G. raimondii, G. hirsutum and G. barbadense, separately. The above ABCGs could be divided into four groups (Ia, Ib, Ic, II). Some ABCG genes such as GhABCG15, whose homologous gene transports cuticular lipid in Arabidopsis, was preferentially expressed in the development of fiber. A weighted gene co-expression network analysis (WGCNA) demonstrated that GhABCG expression was significantly associated with the amount of 16-Hydroxypalmitate (a main component of cutin precursor) in cotton fibers. Further, silencing of GhABCG15 by virus-induced gene silencing (VIGS) in cotton generated brightened and crinkled leaves as well as reduced thickness of cuticle and increased permeability. Chemical composition analysis showed the cutin content in GhABCG15-silenced leaves had decreased while the wax content had increased. Our results provide an insight for better understanding of the role of the Gossypium ABCG family and revealed the essential role of GhABCGs in cotton cutin formation.
通讯机构：
[Wang, YH ] Z;[Zhang, NL ] B;Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China.;Beijing Forestry Univ, State Key Lab Efficient Prod Forest Resources, Beijing 100083, Peoples R China.;Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing 100083, Peoples R China.
关键词：
salt spray;arbuscular mycorrhizal fungi;root trait;salinity tolerance;mycorrhizal colonization
摘要：
Salt spray is a major environmental issue in coastal areas. Cinnamomum camphora is an economically important tree species that grows in the coastal areas of southern China. Arbuscular mycorrhizal fungi (AMF) can alleviate the detrimental effects of abiotic stress on host plants. However, the mechanism by which AMF mitigates the adverse effects of salt spray on C. camphora remains unclear. A pot experiment was conducted in a greenhouse, where C. camphora seedlings were exposed to four AMF regimes (inoculation with sterilized fungi, with Glomus tortuosum, Funneliformis mosseae, either alone or in combination) and three salt spray regimes (applied with distilled water, 7, and 14 mg NaCl cm(-2)) in order to investigate the influence on root functional traits and plant growth. The results showed that higher salt spray significantly decreased the K+ uptake, K+/Na+ ratio, N/P ratio, total dry weight, and salinity tolerance of non-mycorrhizal plants by 37.9%, 71%, 27.4%, 12.7%, and 221.3%, respectively, when compared with control plants grown under non-salinity conditions. Mycorrhizal inoculation, particularly with a combination of G. tortuosum and F. mosseae, greatly improved the P uptake, total dry weight, and salinity tolerance of plants grown under higher salt spray conditions by 51.0%, 36.7%, and 130.9%, respectively, when compared with their counterparts. The results show that AMF can alleviate the detrimental effects of salt spray on C. camphora seedlings. Moreover, an enhanced uptake of K+ and P accounted for the resistance of the plants to salt spray. Therefore, pre-inoculation with a combination of G. tortuosum and F. mosseae to improve nutrient acquisition is a potential method of protecting C. camphora plants against salt spray stress in coastal areas.
期刊：
npj Biofilms and Microbiomes,2023年9(1):62 ISSN：2055-5008
通讯作者：
Xiong, Xia;Yin, YL;Xiong, X;Yin, Jie
作者机构：
[Zhou, Xihong; He, Yiwen; Peng, Can; Yin, Yulong; Xiong, Xia; Liang, Jing; Yin, YL] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China.;[Zhou, Xihong; Yin, Yulong; Xiong, Xia; Liang, Jing; Yin, YL] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China.;[Zhou, Xihong; Xiong, Xia; Huang, Chunxia] Changsha Med Univ, Sch Stomatol, Changsha, Peoples R China.;[He, Yiwen] Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Anim Intestinal Funct & Regulat, Changsha, Peoples R China.;[Chen, Jingqing] Acad Mil Med Sci, Lab Anim Ctr, Beijing, Peoples R China.
通讯机构：
[Xiong, X; Xiong, X ; Yin, YL ] C;[Yin, J] H;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China.;Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China.;Changsha Med Univ, Sch Stomatol, Changsha, Peoples R China.
摘要：
Diarrhea is characterized by alterations in the gut microbiota, metabolites, and host response to these changes. Studies have focused on the role of commensal bacteria in diarrhea; however, the effect of fungi on its pathogenesis remains unexplored. Here, using post-weaned piglets with or without diarrhea, we found an unexpected decrease in the abundance of Candida tropicalis in diarrheal piglets. We also observed increased accumulation of reactive oxygen species (ROS) and the formation of neutrophil extracellular traps (NETs) in the colonic tissues of diarrheal piglets. Using dectin-1-knockout mice, we found that the over-accumulation of ROS killed C. tropicalis by promoting NET formation, which was dependent on dectin-1. The decreased abundance of C. tropicalis resulted in reduced phosphocholine consumption. Then, colonic phosphocholine accumulation drives water efflux by increasing cAMP levels by activating adenylyl cyclase, which promotes the clearance of pathogenic bacteria. Collectively, we demonstrated that phosphocholine is correlated with colonic C. tropicalis and promotes diarrhea and pathogen clearance. Our results suggest that mycobiota colonizing the colon might be involved in maintaining intestinal metabolic homeostasis through the consumption of certain metabolites.
摘要：
Enterococcus faecium (E. faecium) is widely used in foods and is known as a probiotic to treat or prevent diarrhea in pets and livestock. However, the poor resistance of E. faecium to high temperature processing procedures limits its use. Strain domestication is a low-cost and effective method to obtain high-temperature-resistant strains. In this study, heat treatment was performed from 45 & DEG;C to 70 & DEG;C and the temperature was gradually increased by 5 & DEG;C every 3 days. After domestication, the survival rates of the high temperature adaptation strain RS047-wl under 65 & DEG;C water bath for 40 min was 11.5 times higher than WT RS047. Moreover, the saturated fatty acid (SFA) contents in cell membrane and the cell volume significantly increased in the RS047-wl. The combined transcriptomic, metabolomic, and proteomics analysis results showed a significant enhancement of cell wall and membrane synthesis ability in the RS047-wl. In conclusion, one of the main factors contributing to the improved high temperature resistance of RS047-wl was its enhanced ability to synthesize cell wall and membrane, which helped maintain normal cell morphology. Developing a high-temperature-resistant strain and understanding its mechanism enables it to adapt to high temperatures. This lays the groundwork for its future development and application.
期刊：
Plant Physiology and Biochemistry,2023年196:793-806 ISSN：0981-9428
通讯作者：
Liqun Rao<&wdkj&>Qiming Wang
作者机构：
[Zhang, Jinhao; Chen, Rong; Chen, Yanchao; Peng, Guoping; Du, Lihua; Zhu, Qianfeng; Wang, Qiming; Xu, Nan; Rao, Liqun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Zhang, Jinhao; Chen, Rong; Chen, Yanchao; Peng, Guoping; Du, Lihua; Zhu, Qianfeng; Wang, Qiming; Xu, Nan; Rao, Liqun] Hunan Agr Univ, Hunan Engn Lab Good Agr Practice & Comprehens Util, Changsha 410128, Peoples R China.;[Li, Waichin] Educ Univ Hong Kong, Dept Sci & Environm Studies, Hong Kong, Peoples R China.;[Wu, Chuan] Cent South Univ, Sch Met & Environm, Changsha, Peoples R China.
通讯机构：
[Liqun Rao; Qiming Wang] C;College of Bioscience and Biotechnology Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Engineering Laboratory for Good Agricultural Practice and Comprehensive Utilization of Famous-Region Medicinal Plants, Hunan Agricultural University, Changsha, 410128, China
摘要：
Light has important effects on plant metabolism. However, the relationship between the chlorogenic acid (CGA) content and light in plants remains unclear. Here, we investigated the effects of shading treatment on gene expression and CGA content in Lonicera macranthoides Hand.-Mazz. (LM), a widely used medicinal plant. A total of 1891 differentially expressed genes (DEGs) were obtained in flower buds and 819 in leaves in response to light in shading treatment compared to the control sample by RNA-Seq. After shading treatment, the content of CGA in LM leaves decreased significantly by 1.78-fold, the carotenoid content increased, and the soluble sugar and starch contents significantly decreased. WGCNA and the expression of related genes verified by qRT-PCR revealed that CGA synthesis pathway enzyme genes form a co-expression network with genes for carbohydrate synthesis, photosynthesis, light signalling elements, and transcription factor genes (TFs) that affect the accu-mulation of CGA. Through a virus-induced gene silencing (VIGS) system and CGA assay in Nicotiana benthamiana (NB), we determined that downregulation of NbHY5 expression decreased the CGA content in NB leaves. In this study, we found that light provides energy and material for the accumulation of CGA in LM, and light affects the expression of CGA accumulation-related genes. Our results show that different light intensities have multiple effects on leaves and flower buds in LM and are able to coregulate LmHY5 expression and CGA synthesis.
通讯机构：
[Hui Fu] E;Ecology Department, College of Resources & Environments, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha, People’s Republic of China
摘要：
Functional traits of individuals and not just species can regulate ecological interactions with biotic and abiotic environments, yet how individual-level functional diversity (FDind) mediates the environmental effects on community properties (e.g., interaction patterns, connectivity and productivity) remains largely unexplored. Here, we assembled 4432 individuals from 30 macrophyte species across 26 lakes in south China and measured six functional traits -shoot height, specific leaf area, lamina thickness, leaf dry mass content, stem diameter and stem dry mass content-for each individual. We estimated FDind for macrophyte community in each plot using trait probability density framework. Path analysis revealed that functional richness promoted negative interactions (the absolute value of negative: positive cohesion as proxy of community interaction patterns) and thus community productivity at lower total phosphorus (TP) and shallower water, while functional divergence/redundancy reduced negative interaction and thus community productivity at shallower water, lower TP and higher altitude. Functional evenness decreased community productivity at higher TP and deeper water. Functional dissimilarity reduced community connectivity (total cohesion) and thus community productivity at deeper water and higher altitude. Our results highlight that FDind could modify the environmental effects (from local to regional) on the interaction patterns, network connectivity and productivity of macrophyte community.
摘要：
Synthetic red fluorescent protein (RFP) chromophores have emerged as valuable tools for biological imaging and therapeutic applications, but their application in the visualization of endogenous RNA G-quadruplexes (G4s) in living cells has been rarely reported so far. Here, by integrating the group of the excellent G4 dye ThT, we modulate RFP chromophores to create a novel fluorescent probe DEBIT with red emission. DEBIT selectively recognizes the G4 structure with the advantage of strong binding affinity, high selectivity, and excellent photostability. Using DEBIT as a fluorescent indicator, the real-time monitoring of RNA G4 in biological systems can be achieved. In summary, our work expands the application of synthetic RFP chromophores and provides an essential dye category to the classical G4 probes.
作者机构：
[Zeng, Chao-Xi; Qin, Si; Chen, Zhong; Shi, Meng; He, Wen-Jiang] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[He, Wen-Jiang] Infinitus China Co Ltd, Guangzhou 510665, Peoples R China.;[Lv, Cheng-Hao; Qin, Si] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Peoples R China.;[Hou, De-Xing] Kagoshima Univ, Fac Agr, United Grad Sch Agr Sci, Kagoshima 8900065, Japan.
通讯机构：
[Si Qin] C;College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Antibiotic-associated diarrhea (AAD) refers to diarrhea caused by gut microbiota disorders after the use of antibiotics, which seriously threatens the health of humans and animals. Therefore, it is necessary to find an effective therapy to treat AAD. This research aimed to explore the effects of Lactobacillus plantarum H-6 (L. plantarum H-6) and Weissella viridescens J-1 (W. viridescens J-1) on alleviating antibiotic-associated diarrhea induced by lincomycin hydrochloride (LH) in mice. The results show that L. plantarum H-6 could significantly reduce the expression of pro-inflammatory factors such as IL-1β and IL-6 in colon tissue. At the same time, L. plantarum H-6 significantly increased the abundance of Lactobacillus and Akkermansia, decreased the abundance of Bacteroides, and increased the contents of L-tryptophan, LysoPC (20:4 (8Z, 11Z, 14Z, 17Z)), reduced riboflavin, threoninyl-methionine, and N-palmitoyl in serum. However, W. viridescens J-1 had little effect on the treatment of AAD. It can be concluded that L. plantarum H-6 can regulate mice's colonic microbial composition, improve their serum metabolic process, and alleviate antibiotic-associated diarrhea. This research may provide a novel therapeutic option for AAD.
摘要：
The rapid development of nanocellulose and hydrogels has contributed to the development of renewable smart material technologies in recent years. Based on the excellent renewable and biodegradable properties of nanocellulose and the advantages of hydrogels with a high water content, porosity, excellent stretchability and comparable quality to living tissue, nanocellulose hydrogels, which combine the benefits of both, have received significant attention as pioneering materials in the green chemistry revolution. Herein, we present a comprehensive and systematic classification of the nanocellulose hydrogels reported to date in terms of their design-application target relationships. By revealing the intrinsic link between the inherent advantages of nanocellulose and hydrogels, we focus on the applications of nanocellulose hydrogels in medicine, electricity, sensor, environmental governance, food and agriculture and highlight their design innovation advantages. In addition, from the perspective of fundamental issues and practical applications, we provide an in-depth analysis of the shortcomings of the current nanocellulose hydrogels based on four major aspects, including their preparation, platform, application and safety and provide corresponding constructive comments to indicate the future direction of their development. By revealing the intrinsic link between the inherent advantages of nanocellulose and hydrogels, we highlight the applications of nanocellulose hydrogels in medical treatment, electricity, sensor, environmental governance, food, and agriculture.
