期刊：
Journal of Hazardous Materials,2023年454:131535 ISSN：0304-3894
通讯作者：
Zou, Q;Zou, Qi;Wu, C
作者机构：
[Pan, Wei-song; Xiong, Xiao-ran] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Zou, Qi] MEE, South China Inst Environm Sci, Guangzhou 510535, Peoples R China.;[Wu, Chuan; Wu, C; Qi, Yan-ting; Zou, Qi] Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.;[Hu, Min] Guangdong Acad Sci, Inst Eco Environm & Soil Sci, Guangdong Key Lab Integrated Agro Environm Pollut, Guangzhou 510520, Peoples R China.;[Li, Wai-chin] Educ Univ Hong Kong, Dept Sci & Environm Studies, Hong Kong, Peoples R China.
通讯机构：
[Zou, Q ] M;[Wu, C ; Zou, Q] C;MEE, South China Inst Environm Sci, Guangzhou 510535, Peoples R China.;Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.
摘要：
In the current study, a typical Sb mine was selected to explore the microbial community composition and assembly driven by the cocontamination of As/Sb with geographic distance. Our results showed that environmental parameters, especially pH, TOC, nitrate, total and bioavailable As/Sb contents largely affected the microbial community diversity and composition. The total and bioavailable As/Sb levels were significantly positively correlated with the relative abundance of Zavarzinella, Thermosporothrix and Holophaga, while the pH presented a significant negative correlation with the three genera, potentially implying they are important taxonomic groups in acid-mining soils. The cooccurrence network analysis indicated the environmental stress dominated by pH and As/Sb co-contamination affected the microbial modularity and interaction. Meanwhile, Homogeneous selection (HoS, 26.4-49.3%), and drift and others (DR, 27.1∼40.2%) were the most important assembly processes for soil bacterial, and the importance of HoS decreased and the DR increased with geographic distance to the contamination source respectively. Soil pH, nutrient availability, total and bioavailable As/Sb contents significantly affected the HoS and DR processes. This study provides theoretical support for microbial remediation in metal(loid)-contaminated soils.
通讯机构：
[Wang, Zheng; Kang, Jinhe] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan, China<&wdkj&>CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Hunan, China
摘要：
This study was conducted to determine the response of the reproductive hormones and the mTOR/AKT/PI3K pathway in the ovaries of postpartum dairy cows with dietary rumen-protected glucose (RPG). Twelve Holstein cows were randomly assigned to two groups (n = 6/group): the control group (CT) and the RPG group. Blood samples were collected on d 1, 7, and 14 after calving for the gonadal hormone assay. The expression of the gonadal hormones receptors and PI3K/mTOR/AKT pathways were detected using RT-PCR and Western blot. The RPG addition increased the plasma LH, E2, and P4 concentrations on d 14 after calving and upregulated the mRNA and protein expressions of the ER alpha, ER beta, 17 beta-HSD, FSHR, LHR, and CYP17A1 but downregulated StAR expression. Immunohistochemical analysis identified higher expressions of the FSHR and LHR in the ovaries of RPG-fed cows compared to CT cows. Furthermore, the protein expressions of p-AKT/AKT and p-mTOR/mTOR were significantly increased in the ovaries of RPG-fed cows compared to the CT group, but the addition of RPG did not alter the protein expression of p-PI3K/PI3K. In conclusion, the current results indicated that dietary RPG supplementation regulated gonadotropin secretion and stimulated expression of hormone receptors and the mTOR/AKT pathway in the ovaries of early postpartum dairy cows. RPG may be beneficial for the recovery of ovarian activity in post-calving dairy cows.
摘要：
The bacteria pathogen Xanthomonas oryzae pv. oryzae (X-oo) infects rice and causes the severe disease of rice bacteria blight. As the central regulator of the salic acid (SA) signaling pathway, NPR1 is responsible for sensing SA and inducing the expression of pathogen-related (PR) genes in plants. Overexpression of OsNPR1 significantly increases rice resistance to X-oo. Although some downstream rice genes were found to be regulated by OsNPR1, how OsNPR1 affects the interaction of rice-X-oo and alters X-oo gene expression remains unknown. In this study, we challenged the wild-type and OsNPR1-OE rice materials with X-oo and performed dual RNA-seq analyses for the rice and Xoo genomes simultaneously. In X-oo-infected OsNPR1-OE plants, rice genes involved in cell wall biosynthesis and SA signaling pathways, as well as PR genes and nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes, were significantly upregulated compared to rice variety TP309. On the other hand, X-oo genes involved in energy metabolism, oxidative phosphorylation, biosynthesis of primary and secondary metabolism, and transportation were repressed. Many virulence genes of Xoo, including genes encoding components of type III and other secretion systems, were downregulated by OsNPR1 overexpression. Our results suggest that OsNPR1 enhances rice resistance to Xoo by bidirectionally regulating gene expression in rice and X-oo.
摘要：
BACKGROUND AND PURPOSE: The P2X3 receptor, a trimeric ionotropic purinergic receptor, has emerged as a potential therapeutic target for refractory chronic cough (RCC). Nevertheless, Gefapixant/AF-219, the only marketed P2X3 antagonist, might lead taste disorders by modulating the human P2X2/3 (hP2X2/3) heterotrimer. Hence, in RCC drug development, compounds with a strong binding affinity for the hP2X3 homotrimer and a weak binding affinity for the hP2X2/3 heterotrimer hold promise. An example of such a molecule is Sivopixant/S-600918, a clinical phase II RCC candidate with a reduced incidence of taste disturbance compared to Gefapixant. Sivopixant and its analogue, namely (3-(4-((3-chloro-4-isopropoxyphenyl)amino)-3-(4-methylbenzyl)-2,6-dioxo-3,6-dihydro-1,3,5-triazin-1(2H)-yl)propanoic acid (DDTPA), exhibit both high affinity and high selectivity for hP2X3 homotrimers compared to hP2X2/3 heterotrimers. The mechanism underlying the druggable site and its high selectivity remains unclear. EXPERIMENTAL APPROACH: Here, we reveal an allosteric mechanism that distinguishes this drug candidate from other inhibitors of the P2X3 receptors through a combination of chimera construction, site covalent occupation, metadynamics, mutagenesis, and whole-cell recording. KEY RESULTS: Our suggestion is that the high affinity and selectivity of Sivopixant/DDTPA for hP2X3 is determined by the tri-symmetric site located close to the upper vestibule. The substitution of only four amino acids inside the upper body domain of hP2X2 with those of hP2X3 enables the hP2X2/3 heterotrimer to exhibit a similar level of apparent affinity for Sivopixant/DDTPA as the hP2X3 homotrimer. CONCLUSION AND IMPLICATIONS: Thus, from the receptor-ligand recognition perspective, we have elucidated the molecular underpinnings of novel RCC clinical candidates' cough-suppressing properties and reduced side effects, offering a promising avenue for the discovery of novel drugs that specifically target P2X3.
通讯机构：
[Li, H ] U;[Yang, H ; Xiong, XY ; Yang, H] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Crop Utili, Changsha 410128, Peoples R China.;Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518000, Peoples R China.
关键词：
freezing;inhibiting;thereby
摘要：
Cold acclimation is a complex biological process leading to the development of freezing tolerance in plants. In this study, we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative species kumquat [Fortunella margarita (Lour.) Swingle] contributes to its freezing tolerance by minimizing protein degradation. Firstly, we found that only cold-acclimated kumquat plants, despite extensive leaf cellular damage during freezing, were able to resume their normal growth upon stress relief. To dissect the impact of cold acclimation on this anti-freezing performance, we conducted protein abundance assays and quantitative proteomic analysis of kumquat leaves subjected to cold acclimation (4 degrees C), freezing treatment (-10 degrees C) and post-freezing recovery (25 degrees C). FmASP (Against Serine Protease) and several non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of low-temperature treatment. FmASP was further characterized as a robust inhibitor of multiple proteases. In addition, heterogeneous expression of FmASP in Arabidopsis confirmed its positive role in freezing tolerance. Finally, we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation, thereby maintaining essential cellular function for post-freezing recovery. These findings revealed the important role of protease inhibition in freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.
通讯机构：
[Tiean Zhou] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Engineering Technology Research Center for Cell Mechanics and Function Analysis, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
The main sensing techniques used to study myocardial pulsation are electrical impedance sensing (EIS) and by quartz crystal microbalance (QCM). While electrical impedance technology is the gold standard for the study of myocardial pulsation, the clinical application of drugs is being followed up in real time additionally, thus, QCM technology needs to be further developed as a very important class of quality sensor technology. Moreover, the application of EIS, in combination with the QCM, for monitoring myocardial pulsation, has been rarely reported. In this paper, a series of cell growth and adhesion conditions were optimized using rat primary cardiomyocytes, and QCM was used in combination with EIS to monitor the adhesion and the myocardial pulsation ability of the cells in real time. Furthermore, cardiomyocytes that adhered to the QCM and EIS were treated with isoprenaline (ISO), a positive inotropic drug, and verapamil (VRP), a negative inotropic drug. Next, the cell index (CI)-time (T) plots, beating amplitude (BA) and beating rate (BR) of the cardiomyocytes were calculated and changes in these parameters, before and after, dosing were evaluated. The results showed that the QCM technique results were not only consistent with the results obtained with EIS, but also that the QCM technique had a certain degree of sensitivity for the calculation of cardiomyocyte beating. Thus, our findings validate the reliability and validity of the QCM technique for measuring cardiomyocyte beating and drug testing. We hope that further studies would evaluate the application of the QCM technology for clinical use.
关键词：
fermented digestion-promoting Chinese herbs;growing pig;growth properties;serum indicators;gut microbiota
摘要：
Simple Summary Developing new antibiotic substitutes to promote pig growth and health, as well as new feed to improve feed utilization and reduce manure production during the breeding process, have become important ways to solve the current dilemma and promote the pig industry. In this study, we have documented that adding a 2% and 3% digestion-promoting fermented Chinese herbal formula to feed effectively promoted the growth of growing pigs, improved serum immune and antioxidant activities, ameliorated the structure of colonic microbiota, and altered the composition of metabolites in colon contents. In particular, adding a 2% digestion-promoting fermented Chinese herbal formula to feed effectively reduced opportunistic pathogens and increased potential probiotics in the colonic microbiota. This study provides experimental data for the application and feed development of digestion-promoting fermented Chinese herbs for pig production and expands the understanding of the impact of fermented Chinese herbs on the host's gut microbiota and metabolism.Abstract (1) Background: the development of new antibiotic substitutes to promote pig growth and health has become an important way to solve the current dilemma and promote the pig industry. (2) Methods: to assess the effects of a fermented Chinese herbal (FCH) formula on the growth and immunity of growing pigs, 100 Duroc x Landrace x Yorshire three-way crossed growing pigs were randomly divided into control and treatment groups that were fed a basal diet, and a basal diet with 1% (group A), 2% (group B), and 3% (group C) FCH formulas, respectively. A sixty-day formal experiment was conducted, and their growth and serum indices, colonic microbiota, and metabolites were analyzed. (3) Results: the daily gain of growing pigs in groups A, B, and C increased by 7.93%, 17.68%, and 19.61%, respectively, and the feed-to-gain ratios decreased by 8.33%, 15.00%, and 14.58%, respectively. Serum immunity and antioxidant activities were significantly increased in all treatment groups. Particularly, adding a 2% FCH formula significantly changed the colon's microbial structure; the Proteobacteria significantly increased and Firmicutes significantly decreased, and the metabolite composition in the colon's contents significantly changed. (4) Conclusions: these results indicate that the FCH formula is a good feed additive for growing pigs, and the recommended addition ratio was 3%.
作者机构：
[Zongyan Li] School of Biological Science and Technology, Hunan Agricultural University, Changsha, Hunan, China;[Huahai Chen; Aijie Hou; Yeshi Yin] Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, Hunan, China
通讯机构：
[Yeshi Yin] K;Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, Hunan, China
关键词：
Salmonella pullorum;Sanguinarine
摘要：
This work was performed on commercially purchased Salmonella pullorum CVCC519 originally isolated from chicken intestinal content. The Sanguinarine-resistant strain XM3104 was isolated from Sanguinarine-induced CVCC519. To identify possible mechanisms underlying resistance, the complete genomes of CVCC519 and XM3104 were sequenced using PromethION and next-generation sequencing.
作者：
Su, Guirong;Li, Shuzhen;He, Zhiguo;Zhong, Hui;Sun, Wei
期刊：
Journal of Environmental Chemical Engineering,2023年11(6):111509 ISSN：2213-3437
通讯作者：
He, ZG;Zhong, H
作者机构：
[Sun, Wei; Li, Shuzhen; He, ZG; Su, Guirong; He, Zhiguo] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Su, Guirong] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[He, Zhiguo] Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;[Zhong, Hui; Zhong, H] Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
通讯机构：
[He, ZG ; Zhong, H ] C;Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
关键词：
Chalcopyrite;Ethyl xanthate;Adhesion;Biofilm formation;Atomic force microscopy
摘要：
Adhesion and biofilm formation play important roles in the bio-dissolution of metal sulfides, which can initiate dissolution and enhance bioleaching. Ethyl xanthate, a commonly used flotation reagent, remained in flotation concentrates of metal sulfides and may have an impact on the bioleaching of metal sulfides. Therefore, it is important to analyze the effect of ethyl xanthate on microbial initial adhesion and biofilm formation. The results showed that the adhesion percentage of Acidianus manzaensis on chalcopyrite decreased with increasing ethyl xanthate concentration after adsorption equilibrium, due to the toxicity of ethyl xanthate to A. manzaensis. However, a low concentration (0.01 mg/L) of ethyl xanthate had little effect on the growth of A. manzaensis. The hydrophobicity of chalcopyrite increased after the addition of ethyl xanthate, leading to an increase in total interaction energy and adhesion force. The presence of ethyl xanthate inhibited the early growth of biofilm and delayed the formation of microcolonies on chalcopyrite. While biofilm coverage was larger with the addition of ethyl xanthate than that of the control when biofilm was mature. Furthermore, in the presence of ethyl xanthate, more and larger microcolonies were observed on the chalcopyrite surface when biofilm was matured, resulting in an increased corrosion degree and more jarosite produced on the chalcopyrite surface. This study suggested that a low concentration (0.01 mg/L) of ethyl xanthate could promote the dissolution of chalcopyrite, which could be significant for bioleaching of flotation reagent-bearing flotation concentrates.
期刊：
Science of The Total Environment,2023年881:163465 ISSN：0048-9697
通讯作者：
Zhi Liu<&wdkj&>Junhua Chen
作者机构：
[Liu, Zhi; Pan, Jiafeng; Deng, Fang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Chen, Manjia; Pan, Jiafeng; Deng, Fang; Chen, Junhua] Guangdong Acad Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut C, Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Peoples R China.;[Liu, Chengshuai; Chen, Manjia] Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China.;[Liu, Chengshuai] Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Peoples R China.
通讯机构：
[Zhi Liu] C;[Junhua Chen] N;National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
摘要：
Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that cause harmful effects on environmental safety and human health. There is an urgent need to develop an intelligent method for PCBs sensing. In this work, we proposed a logic gate biosensing platform for simultaneous detection of multiple PCBs. 2,3',5,5'-tetrachlorobiphenyl (PCB72) and 3,3',4,4'-tetrachlorobiphenyl (PCB77) were used as the two inputs to construct biocomputing logic gates. We used 0 and 1 to encode the inputs and outputs. The aptamer was used to recognize the inputs and release the trigger DNA. A catalytic hairpin assembly (CHA) module is designed to convert and amplify each trigger DNA into multiple programmable DNA duplexes, which initiate the trans-cleavage activity of CRISPR/Cas12a for the signal output. The activated Cas12 cleaves the BHQ-Cy5 modified single-stranded DNA (ssDNA) to yield the fluorescence reporting signals. In the YES logic gate, PCB72 was used as the only input to carry out the logic operation. In the OR, AND, and INHIBIT logic gates, PCB72 and PCB77 were used as the two inputs. The output signals can be visualized by the naked eye under UV light transilluminators or quantified by a microplate reader. Our constructed biosensing platform possesses the merits of multiple combinations of inputs, intuitive digital output, and high flexibility and scalability, which holds great promise for the intelligent detection of different PCBs.
关键词：
Antioxidant activity;Astilbin;Bioaccessibility;Enzyme inhibition;Molecular dynamic simulation;Natural deep eutectic solvent
摘要：
Astilbin, a natural flavonoid, possesses multiple functionalities, while the poor bioavailability seriously restricts its application in functional food and medicine. Therefore, in this study, a natural deep eutectic solvent (NaDES) with choline chloride: lactic acid (CHCL-LAC) is selected to deliver astilbin by evaluating the bioaccessibility and antioxidant capacity during in vitro gastrointestinal digestion, and the inhibitory effect with underlying mechanism of astilbin-CHCL-LAC against & alpha;-amylase/& alpha;-glucosidase were investigated. The CHCL-LAC showed significant high astilbin bioaccessibility (84.1% bioaccessible) and DPPH and ORAC antioxidant capacity with 75.7% and 57.7% respectively after 3 h in vitro digestion, which may be attributed by hydrogen bond based supramolecule formed between astilbin and CHCL-LAC. Moreover, significant inhibitions of astilbin-CHCL-LAC on & alpha;-amylase (IC50 of 0.67 g/L) and & alpha;-glucosidase (IC50 of 0.64 g/L) were observed in mixed competitive and noncompetitive manners. The dominant binding force between enzymes and astilbin were the hydrogen and hydrophobic interaction. This is the first time that the underlying mechanisms for astilbin delivered by NaDESs were revealed, suggesting that CHCL-LAC-based NaDESs are promising ready-to-use vehicles of natural inhibitors for carbohydrate-hydrolyzing enzymes.
期刊：
FRONTIERS IN NUTRITION,2023年10:1209613 ISSN：2296-861X
通讯作者：
Li, S.
作者机构：
[Li, Siying] College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, Changsha, China
通讯机构：
[Li, S.] C;College of Bioscience and Biotechnology, China
