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Ultrasensitive aptamer biosensor for arsenic (III) detection based on label-free triple-helix molecular switch and fluorescence sensing platform

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成果类型:
期刊论文
作者:
Pan, Jiafeng;Li, Qiong;Zhou, Danhua;Chen, Junhua*
通讯作者:
Chen, Junhua
作者机构:
[Zhou, Danhua; Pan, Jiafeng; Chen, Junhua; Li, Qiong] Guangdong Inst Ecoenvironm Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China.
[Li, Qiong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
[Zhou, Danhua] South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构:
[Chen, Junhua] G
Guangdong Inst Ecoenvironm Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China.
语种:
英文
关键词:
2-amino-5,6,7-trimethyl-1,8-naphthyridine;Aptamer biosensor;Exonuclease III-assisted signal amplification;Label-free;Triple-helix molecular switch
期刊:
Talanta
ISSN:
0039-9140
年:
2018
卷:
189
页码:
370-376
基金类别:
Guangdong Natural Science Funds for distinguished Young Scholar [2016A030306012]; Special Support Program for Young Talent Scholar of Guangdong Province [2015TQ01Z092]; GDAS' Special Project of Science and Technology Development [2017GDASCX-0405]; Science and Technology Program of Guangdong Province [2016B070701015, 2016B020204003]; SPICC Program [2016GDASPT-0105]
机构署名:
本校为其他机构
院系归属:
生物科学技术学院
摘要:
Arsenic ion is a well-known harmful heavy element widely existing in the environment. Arsenic pollution occurring frequently has become increasing a serious worldwide threat to human health and the environment. The development of sensitive and reliable methods to detect As3+ in water is of great importance to biochemical research and monitoring applications. Herein, a label-free fluorescence sensing platform was elaborately designed for As3+ monitoring using exonuclease III (Exo III)-assisted cascade target recycling amplification strategy. The triple-helix molecular switch was employed as the...

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