作者机构：
[Liu, Weijiao; He, Qing; Li, Dantong; Zou, Yawen; Yu, Beilei; Zhan, Yang; Wu, Jing; Cao, Siyu; Li, Zhoumian; Jiang, You; Yang, Yi] Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics (LFP) & Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, China;[Yu, Wanting] College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China;[Tian, Chuanwen] Key Laboratory of Animal Biosafety Risk Prevention and Control (North), P.R. China, Shanghai Veterinary Research Institute, Biosafety Research Center, Chinese Academy of Agricultural Sciences, Shanghai, China;[Wang, Naidong] Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics (LFP) & Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. Electronic address: naidongwang@hunau.edu.cn
通讯机构：
[Wang, Naidong] H;Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics (LFP) & Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. Electronic address:
关键词：
Cap;HnRNP K;Mitochondrial apoptosis;Porcine circovirus type 3
摘要：
Porcine circovirus type 3 (PCV3) is a globally emerging circovirus affecting pigs and other animals. The capsid protein (Cap) is the sole structural protein of PCV, with a crucial role in virus evolution and pathogenesis. Through interactions with host factors, Cap enables viral entry, transport, and replication while modifying various cellular processes. Cap protein-induced apoptosis has important implications for viral pathogenesis, but remains poorly defined. Herein, we demonstrated for the first time that PCV3 Cap induced cell cycle arrest of PK-15 cells in S-phase and initiated apoptosis via a mitochondrial Caspase-9-dependent pathway. Truncation analysis localized the apoptotic determinant to the N-terminal 1-34 aa of PCV3 Cap and heterogeneous nuclear ribonucleoprotein K (hnRNP K) was identified as a host protein that binds to PCV3 Cap. Overexpression of hnRNP K reduced PCV3 Cap-induced release of Cyt-c into the cytoplasm, implying a regulatory role in apoptosis. Based on structural modelling and molecular docking, amino acids at sites 24 and 27 of Cap from PCV3 variants, which define genotypes (PCV3a/b/c), affected binding with hnRNP K. Specifically, PCV3c Cap (V24/K27 and V24/R27) had higher affinity than PCV3a Cap (A24/R27) or PCV3b Cap (A24/K27), consistent with its superior apoptosis-inducing capacity compared to PCV3a/b variants, highlighting the importance of Cap interactions with hnRNP K. In summary, we identified novel molecular determinants of PCV3 pathogenesis that will inform development of vaccines and diagnostics.
Porcine circovirus type 3 (PCV3) is a globally emerging circovirus affecting pigs and other animals. The capsid protein (Cap) is the sole structural protein of PCV, with a crucial role in virus evolution and pathogenesis. Through interactions with host factors, Cap enables viral entry, transport, and replication while modifying various cellular processes. Cap protein-induced apoptosis has important implications for viral pathogenesis, but remains poorly defined. Herein, we demonstrated for the first time that PCV3 Cap induced cell cycle arrest of PK-15 cells in S-phase and initiated apoptosis via a mitochondrial Caspase-9-dependent pathway. Truncation analysis localized the apoptotic determinant to the N-terminal 1-34 aa of PCV3 Cap and heterogeneous nuclear ribonucleoprotein K (hnRNP K) was identified as a host protein that binds to PCV3 Cap. Overexpression of hnRNP K reduced PCV3 Cap-induced release of Cyt-c into the cytoplasm, implying a regulatory role in apoptosis. Based on structural modelling and molecular docking, amino acids at sites 24 and 27 of Cap from PCV3 variants, which define genotypes (PCV3a/b/c), affected binding with hnRNP K. Specifically, PCV3c Cap (V24/K27 and V24/R27) had higher affinity than PCV3a Cap (A24/R27) or PCV3b Cap (A24/K27), consistent with its superior apoptosis-inducing capacity compared to PCV3a/b variants, highlighting the importance of Cap interactions with hnRNP K. In summary, we identified novel molecular determinants of PCV3 pathogenesis that will inform development of vaccines and diagnostics.
作者机构：
[Wu, Simin; Fang, Xinyu; Zhao, Jinfeng] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, 410128, China;[Wu, Simin; Fang, Xinyu; Zhao, Jinfeng] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China;[Liao, Peng] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China;[Liao, Peng] Hunan Provincial Key Laboratory of the TCM Agricultural Biogenomics, Changsha Medical University, Changsha, Hunan, 410219, China. Electronic address: liaopeng@isa.ac.cn;[Guan, Guiping] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, 410128, China. Electronic address: guanguiping@hunau.edu.cn
通讯机构：
[Guan, Guiping] C;[Liao, Peng] H;Hunan Provincial Key Laboratory of the TCM Agricultural Biogenomics, Changsha Medical University, Changsha, Hunan, 410219, China. Electronic address:;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, 410128, China. Electronic address:
摘要：
Triclosan (TCS) and triclocarban (TCC) are widely used as antimicrobial agents in personal care products. Their widespread use has become a potential environmental contaminant. This review reviews the mechanisms of intestinal toxicity of TCS and TCC and their potential nutritional intervention strategies. TCS and TCC can be metabolized to glucuronic acid conjugates in the host and subsequently uncoupled by microorganisms in the intestine to regenerate free forms of TCS and TCC. TCS and TCC are unique metabolic pathways that lead to accumulation in the gut, altering the structure of intestinal flora, increasing the relative abundance of pathogenic bacteria, while reducing the abundance of beneficial bacteria, thereby disrupting the balance of intestinal flora. In addition, they can interfere with the self-renewal and differentiation of ISCs, thereby weakening intestinal barrier function. TCS and TCC can also activate the TLR4-NFκB signaling pathway, inducing and exacerbating inflammatory responses. These mechanisms together lead to intestinal toxicity and have a significant negative impact on intestinal health. In order to cope with the intestinal toxicity caused by these mechanisms of action, this paper believes that prebiotics, probiotics, vitamins, minerals and herbal extracts can be used as potential nutritional interventions to reduce the intestinal toxicity of TCS and TCC by regulating intestinal microbiota, enhancing intestinal barrier function and inhibiting inflammatory response. Although preliminary studies have shown the potential benefits of these interventions, their specific efficacy and safety still need further study.
Triclosan (TCS) and triclocarban (TCC) are widely used as antimicrobial agents in personal care products. Their widespread use has become a potential environmental contaminant. This review reviews the mechanisms of intestinal toxicity of TCS and TCC and their potential nutritional intervention strategies. TCS and TCC can be metabolized to glucuronic acid conjugates in the host and subsequently uncoupled by microorganisms in the intestine to regenerate free forms of TCS and TCC. TCS and TCC are unique metabolic pathways that lead to accumulation in the gut, altering the structure of intestinal flora, increasing the relative abundance of pathogenic bacteria, while reducing the abundance of beneficial bacteria, thereby disrupting the balance of intestinal flora. In addition, they can interfere with the self-renewal and differentiation of ISCs, thereby weakening intestinal barrier function. TCS and TCC can also activate the TLR4-NFκB signaling pathway, inducing and exacerbating inflammatory responses. These mechanisms together lead to intestinal toxicity and have a significant negative impact on intestinal health. In order to cope with the intestinal toxicity caused by these mechanisms of action, this paper believes that prebiotics, probiotics, vitamins, minerals and herbal extracts can be used as potential nutritional interventions to reduce the intestinal toxicity of TCS and TCC by regulating intestinal microbiota, enhancing intestinal barrier function and inhibiting inflammatory response. Although preliminary studies have shown the potential benefits of these interventions, their specific efficacy and safety still need further study.
关键词：
auxin;flavanones;phytohormones;root zone chilling stress;terminal flowering formation
摘要：
A low-temperature condition in a root zone is a major abiotic stress that threatens cucumber (Cucumis sativus L.) growth and development, yet the molecular mechanism by which the leaf reacts to root zone chilling stress remains largely unknown. In this study, we applied three temperature treatments, including room temperature (20 degrees C-22 degrees C), suboptimal temperature (13 degrees C-15 degrees C), and low temperature (8 degrees C-10 degrees C), to investigate how root zone chilling affects hormone dynamics, metabolomics, and transcriptomics in the leaves of the cucumber variety "Jinyou 35", the main cultivar in northwest and southwest China. Through integrative physiological and biochemical analysis, auxin emerges as the most significant accumulated hormone, accounting for 88% in room temperature-treated leaves (RL), 99% in suboptimal temperature-treated leaves (SL), and 94% in low-temperature-treated leaves (LL). Under chilling stress, flavanones were the most abundant metabolite in cucumber leaves, constituting over 50% of total metabolites, while phenolic acids showed a marked decrease. Several differentially expressed transcription factors (DETFs), such as LOB (CsaV3_3G020650), MYB (CsaV3_3G043510), and bHLH (CsaV3_2G005070 and CsaV3_4G029740), were upregulated in SL and LL, potentially enhancing cucumber's defense against chilling injury. Additionally, terminal flower formation was observed under suboptimal and low-temperature conditions, with CsFT expression in SL and LL lower than in RL, and a significant negative correlation observed between CsFT and CsNAC6. These findings deepen our understanding of cucumber's resilience mechanisms to root zone chilling stress, shedding light on its cold tolerance strategies.
摘要：
This study investigated the release of taste-active peptides from soybean isolate catalyzed by proteases from Aspergillus flavus strains. The frequent presence of Leu at the C-terminus and the hydrophilic amino acids at the N-terminus related to bitterness and umami taste of hydrolysates, respectively. Detailly, H40650 demonstrating high umami, low bitterness, and a high peptide yield. During the hydrolysis of H40650, umami taste changes were associated with the content of peptides <1 kDa and 28 umami peptides, while bitterness was linked to the content of peptides <3 kDa and 30 bitter peptides. Additionally, most taste-active peptides were derived from the 7S globulin α subunit and 11S globulin G2. The protease produced by CGMCC 40650 has marked specificity for the region of 281–333 amino acids in protein P0D15, which contained 24.53 % umami amino acids. These findings could offer new insights into the preparation of taste-active peptides.
This study investigated the release of taste-active peptides from soybean isolate catalyzed by proteases from Aspergillus flavus strains. The frequent presence of Leu at the C-terminus and the hydrophilic amino acids at the N-terminus related to bitterness and umami taste of hydrolysates, respectively. Detailly, H40650 demonstrating high umami, low bitterness, and a high peptide yield. During the hydrolysis of H40650, umami taste changes were associated with the content of peptides <1 kDa and 28 umami peptides, while bitterness was linked to the content of peptides <3 kDa and 30 bitter peptides. Additionally, most taste-active peptides were derived from the 7S globulin α subunit and 11S globulin G2. The protease produced by CGMCC 40650 has marked specificity for the region of 281–333 amino acids in protein P0D15, which contained 24.53 % umami amino acids. These findings could offer new insights into the preparation of taste-active peptides.
摘要：
In humans, cadmium (Cd) toxicity caused by contaminated environments is associated with numerous chronic diseases. Breeding rice with low Cd accumulation is now deemed critical for sustainable agriculture development. Here, we elucidate the crucial functions of UCLACYANIN 23 (UCL23), a small copper protein, in Cd absorption, tolerance, and accumulation through modulation of reactive oxygen signals in rice. Additionally, we demonstrate that WRKY51 binds to promoters of UCL23 and miR528, a post-transcriptional regulator of UCL23 , thereby contributing to Cd regulation in a dual-modulatory manner. Furthermore, we show that the natural variation of UCL23 is important for the differential accumulation of Cd in rice grains. Finally, we reveal that Indica rice harboring the major Japonica haplotype of UCL23 significantly reduces Cd uptake in roots and Cd accumulation in grains. Together, our study not only reveals a regulatory cascade in Cd regulation but also provides valuable resources for breeding low-Cd rice cultivars.
In humans, cadmium (Cd) toxicity caused by contaminated environments is associated with numerous chronic diseases. Breeding rice with low Cd accumulation is now deemed critical for sustainable agriculture development. Here, we elucidate the crucial functions of UCLACYANIN 23 (UCL23), a small copper protein, in Cd absorption, tolerance, and accumulation through modulation of reactive oxygen signals in rice. Additionally, we demonstrate that WRKY51 binds to promoters of UCL23 and miR528, a post-transcriptional regulator of UCL23 , thereby contributing to Cd regulation in a dual-modulatory manner. Furthermore, we show that the natural variation of UCL23 is important for the differential accumulation of Cd in rice grains. Finally, we reveal that Indica rice harboring the major Japonica haplotype of UCL23 significantly reduces Cd uptake in roots and Cd accumulation in grains. Together, our study not only reveals a regulatory cascade in Cd regulation but also provides valuable resources for breeding low-Cd rice cultivars.
摘要：
A novel enzyme-modified cheddar cheese was prepared and the molecular mechanism of cheese flavor compensation by synergistic action of cell-free extracts and enzyme systems was investigated. By comparing five different protease-peptidase combinations, the group of neutral protease and flavor protease was found to increase the total leucine, valine, and isoleucine content (17.056 ± 0.136 g/kg) and the soluble nitrogen content was up to the level of a 12-month-matured cheese. Molecular docking and molecular dynamics simulations demonstrated their mode of action on four monomeric caseins. Adding a cell-free extract resulted in volatile flavor substances in the enzyme-modified cheese that were closest to those in the 12-month-matured cheese. This might be due to the flavor compensation effect of the conversion of leucine to 3-methylbutyraldehyde by transaminases and decarboxylases, and the conversion of 3-methylbutyric acid to 3-methylbutyraldehyde by ketoacid dehydrogenase and aldehyde dehydrogenase. This is essential for the enzyme modified cheddar cheese preparation.
A novel enzyme-modified cheddar cheese was prepared and the molecular mechanism of cheese flavor compensation by synergistic action of cell-free extracts and enzyme systems was investigated. By comparing five different protease-peptidase combinations, the group of neutral protease and flavor protease was found to increase the total leucine, valine, and isoleucine content (17.056 ± 0.136 g/kg) and the soluble nitrogen content was up to the level of a 12-month-matured cheese. Molecular docking and molecular dynamics simulations demonstrated their mode of action on four monomeric caseins. Adding a cell-free extract resulted in volatile flavor substances in the enzyme-modified cheese that were closest to those in the 12-month-matured cheese. This might be due to the flavor compensation effect of the conversion of leucine to 3-methylbutyraldehyde by transaminases and decarboxylases, and the conversion of 3-methylbutyric acid to 3-methylbutyraldehyde by ketoacid dehydrogenase and aldehyde dehydrogenase. This is essential for the enzyme modified cheddar cheese preparation.
摘要：
The influence of the airflow field around the conditioning roll of a mower conditioner on the forage harvesting process cannot be ignored. Full factorial simulation tests of the airflow field of a single conditioning roll and double conditioning roll were carried out, respectively, by taking the roll type, roll rotational velocity, and roll clearance as factors. The results show that the average airflow velocity around the conditioning rolls has a linear positive correlation with the roll velocity and an exponential negative correlation with the distance from the centre of the conditioning rolls in the single roll case. In the case of double rolls, the airflow around the conditioning rolls increase with the velocity of the rolls and decrease with the increase of the clearance between the rolls. The results of the field verification test show that, compared with the measured values for the prototype, the average error of the average velocity of the airflow around the single roll, predicted by the regression model, is 6.35%. The average error of the predicted velocity of the back-feeding airflow of the double roll is 5.86%. The results are reliable and could provide a reference for the optimised design of the conditioning roll.
作者机构：
[Wang, Yu; Cai, Jiazhen; Lin, Hao] School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China;Tea industry Research Institute, Fujian Eight Horses Tea Co., Ltd, Quanzhou 362442, PR China;[Liu, Zhonghua] National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, PR China;[Ouyang, Qin] School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China<&wdkj&>Tea industry Research Institute, Fujian Eight Horses Tea Co., Ltd, Quanzhou 362442, PR China
通讯机构：
[Qin Ouyang] S;School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China<&wdkj&>Tea industry Research Institute, Fujian Eight Horses Tea Co., Ltd, Quanzhou 362442, PR China
摘要：
The withering process is a critical stage in developing the aroma profile of black tea. In this study, we presented an eco-friendly cellulose film-based colorimetric sensor array (CSA) for detecting volatile organic compounds (VOCs) and assessing withering stages using deep learning. TiO 2 was attached to the cellulose film surface, resulting in a self-cleaning TiO 2 -cellulose film. Functionalized cellulose film featuring hydrophobic non-sensing areas were fabricated via site-specific deposition of octadecyltrichlorosilane (OTS). The OTS/TiO 2 -CSA was prepared by drop-coating multiple dyes onto the hydrophilic sensing area of the functionalized cellulose film, exhibiting improved humidity resistance. By assisting with a deep learning model (Long Short-Term Memory), the OTS/TiO 2 -CSA achieved 90 % accuracy in identifying withering stages. Notably, dyes on the OTS/TiO 2 -CSA surface degraded under limited UV exposure, most exceeding 70 % degradation. This study introduces a fabrication strategy for a smart, eco-friendly OTS/TiO 2 -CSA, while demonstrating its potential as a sustainable tool for monitoring tea withering stages.
The withering process is a critical stage in developing the aroma profile of black tea. In this study, we presented an eco-friendly cellulose film-based colorimetric sensor array (CSA) for detecting volatile organic compounds (VOCs) and assessing withering stages using deep learning. TiO 2 was attached to the cellulose film surface, resulting in a self-cleaning TiO 2 -cellulose film. Functionalized cellulose film featuring hydrophobic non-sensing areas were fabricated via site-specific deposition of octadecyltrichlorosilane (OTS). The OTS/TiO 2 -CSA was prepared by drop-coating multiple dyes onto the hydrophilic sensing area of the functionalized cellulose film, exhibiting improved humidity resistance. By assisting with a deep learning model (Long Short-Term Memory), the OTS/TiO 2 -CSA achieved 90 % accuracy in identifying withering stages. Notably, dyes on the OTS/TiO 2 -CSA surface degraded under limited UV exposure, most exceeding 70 % degradation. This study introduces a fabrication strategy for a smart, eco-friendly OTS/TiO 2 -CSA, while demonstrating its potential as a sustainable tool for monitoring tea withering stages.
期刊：
Food Research International,2025年:116555 ISSN：0963-9969
通讯作者：
Lizheng Xiao<&wdkj&>Zhonghua Liu<&wdkj&>Wei Quan
作者机构：
[Silin Xie; Jiahui Bo] National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China;[Xirui Zhou; Jie Li] Key Laboratory of Tea Science of Ministry of Education and Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China;Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China;[Maiquan Li] College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China;[Yong Lin; Zhonghua Liu] National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education and Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Lizheng Xiao; Zhonghua Liu; Wei Quan] N;National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education and Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education and Co-Innovation Centre of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China
摘要：
White tea (WT) has been reported to confer various health benefits, but its role in inflammatory bowel disease (IBD) has not been fully investigated. Jinhua white tea (JWT), produced using the unique “flowering” technology by Eurotium cristatum , enhancing the flavor and quality of WT. Whether this new technology provides better bioactive benefits also worth further exploring. The present study investigated the protective effects of WT and JWT against dextran sodium sulfate (DSS)-induced IBD. The results showed WT and JWT mitigated oxidative stress and colonic inflammation via NF- κ B and MAPK signaling pathways, upregulating intestinal tight junction protein expression and immune cell counts. These effects were evidenced by improvements in pathological phenotypes, disease activity index, and colon length in IBD mice, with JWT showing superior results. Both WT and JWT restored microbial diversity and improved gut microbiota composition in IBD mice. JWT showed a more pronounced effect on correcting microbial imbalances and abnormal host metabolism by stimulating metabolic pathways associated with amino acid metabolism, energy metabolism, and secondary metabolite biosynthesis. Therefore, WT, particularly JWT, shows potential for adjuvant treatment of colitis, which might be attributed to the enrichments in oxidation products of catechins such as theabrownins and some secondary metabolites including alkaloids, flavonoids and gallic acid obtained through the “flowering” technology. Further identifying and isolating key compounds and clinical trials are required to assess the therapeutic effects and mechanisms of JWT on IBD in humans.
White tea (WT) has been reported to confer various health benefits, but its role in inflammatory bowel disease (IBD) has not been fully investigated. Jinhua white tea (JWT), produced using the unique “flowering” technology by Eurotium cristatum , enhancing the flavor and quality of WT. Whether this new technology provides better bioactive benefits also worth further exploring. The present study investigated the protective effects of WT and JWT against dextran sodium sulfate (DSS)-induced IBD. The results showed WT and JWT mitigated oxidative stress and colonic inflammation via NF- κ B and MAPK signaling pathways, upregulating intestinal tight junction protein expression and immune cell counts. These effects were evidenced by improvements in pathological phenotypes, disease activity index, and colon length in IBD mice, with JWT showing superior results. Both WT and JWT restored microbial diversity and improved gut microbiota composition in IBD mice. JWT showed a more pronounced effect on correcting microbial imbalances and abnormal host metabolism by stimulating metabolic pathways associated with amino acid metabolism, energy metabolism, and secondary metabolite biosynthesis. Therefore, WT, particularly JWT, shows potential for adjuvant treatment of colitis, which might be attributed to the enrichments in oxidation products of catechins such as theabrownins and some secondary metabolites including alkaloids, flavonoids and gallic acid obtained through the “flowering” technology. Further identifying and isolating key compounds and clinical trials are required to assess the therapeutic effects and mechanisms of JWT on IBD in humans.
关键词：
key taxa;microbial community;pepper growth;synthetic microbial community
摘要：
Synthetic microbial community (SynCom) application is efficient in promoting crop yield and soil health. However, few studies have been conducted to enhance pepper growth via modulating rhizosphere microbial communities by SynCom application. This study aimed to investigate how SynCom inoculation at the seedling stage impacts pepper growth by modulating the rhizosphere microbiome using high-throughput sequencing technology. SynCom inoculation significantly increased shoot height, stem diameter, fresh weight, dry weight, chlorophyll content, leaf number, root vigor, root tips, total root length, and root-specific surface area of pepper by 20.9%, 36.33%, 68.84%, 64.34%, 29.65%, 27.78%, 117.42%, 35.4%, 21.52%, and 39.76%, respectively, relative to the control. The Chao index of the rhizosphere microbial community and Bray-Curtis dissimilarity of the fungal community significantly increased, while Bray-Curtis dissimilarity of the bacterial community significantly decreased by SynCom inoculation. The abundances of key taxa such as Scedosporium, Sordariomycetes, Pseudarthrobacter, norankSBR1031, and norankA4b significantly increased with SynCom inoculation, and positively correlated with indices of pepper growth. Our findings suggest that SynCom inoculation can effectively enhance pepper growth and regulate root morphology by regulating rhizosphere microbial communities and increasing key taxa abundance like Sordariomycetes and Pseudarthrobacter, thereby benefiting nutrient acquisition, resistance improvement, and pathogen resistance of crops to ensure sustainability.
期刊：
Journal of Advanced Research,2025年70:323-338 ISSN：2090-1232
通讯作者：
Xuan Zhao<&wdkj&>Miaomiao Wu<&wdkj&>Yaoyao Xia
作者机构：
[Li, Yikun; Chen, Yating; "Liu, Yun] College of Animal Science and Technology, Southwest University, Chongqing 400715, China;[Wu, Zifang] College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China;[Li, Yikun] College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China;[Zhao, Xuan] College of Animal Science and Technology, Southwest University, Chongqing 400715, China. Electronic address: zhaoxuann@swu.edu.cn;[Wu, Miaomiao] Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China. Electronic address: miaomiaowu0316@hunau.edu.cn
通讯机构：
[Xuan Zhao; Yaoyao Xia] C;[Miaomiao Wu] A;College of Animal Science and Technology, Southwest University, Chongqing 400715, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
摘要：
Background Endothelial cell (EC) metabolism plays a crucial role in the process of angiogenesis. Intrinsic metabolic events such as glycolysis, fatty acid oxidation, and glutamine metabolism, support secure vascular migration and proliferation, energy and biomass production, as well as redox homeostasis maintenance during vessel formation. Nevertheless, perturbation of EC metabolism instigates vascular dysregulation-associated diseases, especially cancer.
Endothelial cell (EC) metabolism plays a crucial role in the process of angiogenesis. Intrinsic metabolic events such as glycolysis, fatty acid oxidation, and glutamine metabolism, support secure vascular migration and proliferation, energy and biomass production, as well as redox homeostasis maintenance during vessel formation. Nevertheless, perturbation of EC metabolism instigates vascular dysregulation-associated diseases, especially cancer.
Aim of review In this review, we aim to discuss the metabolic regulation of angiogenesis by EC metabolites and metabolic enzymes, as well as prospect the possible therapeutic opportunities and strategies targeting EC metabolism.
In this review, we aim to discuss the metabolic regulation of angiogenesis by EC metabolites and metabolic enzymes, as well as prospect the possible therapeutic opportunities and strategies targeting EC metabolism.
Key scientific concepts of review In this work, we discuss various aspects of EC metabolism considering normal and diseased vasculature. Of relevance, we highlight that the implications of EC metabolism-targeted intervention (chiefly by metabolic enzymes or metabolites) could be harnessed in orchestrating a spectrum of pathological angiogenesis-associated diseases.
In this work, we discuss various aspects of EC metabolism considering normal and diseased vasculature. Of relevance, we highlight that the implications of EC metabolism-targeted intervention (chiefly by metabolic enzymes or metabolites) could be harnessed in orchestrating a spectrum of pathological angiogenesis-associated diseases.
摘要：
This work focused on the mechanism of photogenerated hole (h+) mediating the generation of active species in visible-light-assisted photocatalytic peroxymonosulfate (PMS) activation. Ferrous gluconate derived iron-doped carbon dots (Fe-CDs) were synthesized to investigate the vital role of h+ in the process of photocatalytic PMS activation for acetaminophen degradation by quenching experiments, ESR spectra, and DFT calculations. Within 20 minutes, Fe-CDs/PMS/Vis system can achieve a 100 % degradation of acetaminophen (15 μM) with low-addition of PMS (0.2 mM) and Fe-CDs (12 μg/L). The Fe3+ sites on Fe-CDs reduce the activation energy for the generation of SO5−• from PMS, increase the concentration and utilization of h+ in the system, and make the direct activation of PMS by h+ be the dominant pathway to mediate the generation of •OH and 1O2. This work provides mechanistic insights for further research on h+ mediated regulation of active species in photocatalytic PMS activation process for micropollutants removal.
This work focused on the mechanism of photogenerated hole (h+) mediating the generation of active species in visible-light-assisted photocatalytic peroxymonosulfate (PMS) activation. Ferrous gluconate derived iron-doped carbon dots (Fe-CDs) were synthesized to investigate the vital role of h+ in the process of photocatalytic PMS activation for acetaminophen degradation by quenching experiments, ESR spectra, and DFT calculations. Within 20 minutes, Fe-CDs/PMS/Vis system can achieve a 100 % degradation of acetaminophen (15 μM) with low-addition of PMS (0.2 mM) and Fe-CDs (12 μg/L). The Fe3+ sites on Fe-CDs reduce the activation energy for the generation of SO5−• from PMS, increase the concentration and utilization of h+ in the system, and make the direct activation of PMS by h+ be the dominant pathway to mediate the generation of •OH and 1O2. This work provides mechanistic insights for further research on h+ mediated regulation of active species in photocatalytic PMS activation process for micropollutants removal.
