通讯机构:
[Wang, RR ; Ding, SH ] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;Hunan Univ, Coll Biol, Changsha 410125, Peoples R China.
关键词:
Starch film;Janus structure;Rapid self-assembly;Fresh-cut fruits and vegetables preservation
摘要:
Starch-based films are promising biodegradable materials for food packaging but are limited by hydrophilicity and poor functional properties. Herein, this study proposed a citral nanoemulsion (NEs)-mediated interfacial engineering strategy to construct a self-assembled Janus starch film (NE-S/M) with dual hydrophobic-active functionality for fresh-cut fruits and vegetables preservation. Specifically, starch extracted from waste rice was composited with citral NEs to form bioactive films (NE-S), which were further rapidly self-assembled with myristic acid (MA) to create a Janus hydrophobic structure. Structural characterization and molecular dynamics simulation revealed that citral NEs acted as molecular bridges to enhance the MA adsorption energy barrier, enabling MA to self-assemble into a dense hydrophobic layer via free deposition. Especially, the 10 %NE-S/M Janus film exhibited exceptional hydrophobicity (water contact angle: 118.22°), UV-blocking (0 % transmittance at 200–400 nm), mechanical robustness (tensile strength enhanced by 180.75 %), and barrier properties (water vapor permeability reduced by 62.39 %). Furthermore, the Janus film synergized the outstanding antioxidant and antimicrobial properties of citral. Compared to starch film, the 10 %NE-S/M Janus film exhibited a three-fold increase in free radical scavenging capacity and notable inhibition on Staphylococcus aureus , Escherichia coli , and Botrytis cinerea with relative inhibition rates of 98.63 %, 91.56 %, and 42.53 %, respectively. Preservation tests demonstrated that 10 %NE-S/M film significantly reduced weight loss, suppressed microbial proliferation, and delayed quality deterioration in fresh-cut kiwifruit and chili peppers throughout storage. Crucially, this rapid self-assembly strategy successfully achieved hydrophobicity and bioactive properties in starch-based film and offered a sustainable solution for active food packaging with multifunctional performance.
Starch-based films are promising biodegradable materials for food packaging but are limited by hydrophilicity and poor functional properties. Herein, this study proposed a citral nanoemulsion (NEs)-mediated interfacial engineering strategy to construct a self-assembled Janus starch film (NE-S/M) with dual hydrophobic-active functionality for fresh-cut fruits and vegetables preservation. Specifically, starch extracted from waste rice was composited with citral NEs to form bioactive films (NE-S), which were further rapidly self-assembled with myristic acid (MA) to create a Janus hydrophobic structure. Structural characterization and molecular dynamics simulation revealed that citral NEs acted as molecular bridges to enhance the MA adsorption energy barrier, enabling MA to self-assemble into a dense hydrophobic layer via free deposition. Especially, the 10 %NE-S/M Janus film exhibited exceptional hydrophobicity (water contact angle: 118.22°), UV-blocking (0 % transmittance at 200–400 nm), mechanical robustness (tensile strength enhanced by 180.75 %), and barrier properties (water vapor permeability reduced by 62.39 %). Furthermore, the Janus film synergized the outstanding antioxidant and antimicrobial properties of citral. Compared to starch film, the 10 %NE-S/M Janus film exhibited a three-fold increase in free radical scavenging capacity and notable inhibition on Staphylococcus aureus , Escherichia coli , and Botrytis cinerea with relative inhibition rates of 98.63 %, 91.56 %, and 42.53 %, respectively. Preservation tests demonstrated that 10 %NE-S/M film significantly reduced weight loss, suppressed microbial proliferation, and delayed quality deterioration in fresh-cut kiwifruit and chili peppers throughout storage. Crucially, this rapid self-assembly strategy successfully achieved hydrophobicity and bioactive properties in starch-based film and offered a sustainable solution for active food packaging with multifunctional performance.
摘要:
Introduction: Impaired intestinal immune function is commonly observed in neonates with intrauterine growth retardation (IUGR), yet its underlying mechanisms and regulatory pathways remain poorly understood. Therefore, we aimed to investigate gene regulatory patterns and microbiota alterations in IUGR piglets.<&wdkj&>Methods: Three newborn IUGR piglets and three normal littermates were selected from the same sow and sacrificed at seven days of age. Ileal digesta was collected for 16S rRNA amplicon sequencing (16S-seq), and ileum segments were dissociated for single-cell RNA sequencing (scRNA-seq).<&wdkj&>Results: The scRNA-seq results revealed a reduced proportion of plasma B cells in IUGR piglets, along with alterations in the distribution of various T cell subsets. KEGG pathway analysis further indicated a downregulation of the B cell receptor signaling pathway in B cells from IUGR piglets. In contrast, both the T cell receptor signaling pathway and antigen processing and presentation were attenuated in T cells. Pseudotime trajectory analysis suggested that the differentiation of B cells was impaired in IUGR piglets. SCENIC analysis revealed that GATA3, IRF2, and BCL11A were downregulated in T cells of IUGR piglets. The 16S-seq results revealed that α-diversity was lower in IUGR piglets. At the genus level, the relative abundance of Prevotella was significantly lower in IUGR piglets.<&wdkj&>Discussion: Significant changes were identified in the proportions of B and T cells, their associated signaling pathways, and intestinal microbiota composition in IUGR piglets, suggesting underlying immune dysfunction and dysbiosis.<&wdkj&>Conclusion: We identified novel immune-related transcription factors and key microbes as potential therapeutic targets, shedding light on strategies for preventing and treating IUGR.
期刊:
Journal of Colloid and Interface Science,2026年702(Pt 1):138837 ISSN:0021-9797
通讯作者:
Gao, Zhongfeng
作者机构:
[Li, Xinyuan; Sui, Xin; Liu, Fanglan; Li, Yingqi; Li, Na; Mai, Xi; Wang, Cheng; Long, Qinghong] School of Pharmacy, Jiangxi Medical College, Nanchang University, Jiangxi Province Key Laboratory of New Drug Evaluation and Transformation, Nanchang 330031, PR China;[Li, Yanlei] Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China;[Liu, Shigang] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, PR China;[Gao, Zhongfeng] Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.. Electronic address: chm_gaozf@ujn.edu.cn;[Xia, Fan] Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, PR China
通讯机构:
[Gao, Zhongfeng] K;Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.. Electronic address:
摘要:
Employing antioxidant nanozymes to eliminate reactive oxygen species (ROS) is a promising strategy for alleviating oxidative stress. However, most current nanozymes struggle to balance catalytic efficacy with biosafety, limiting their clinical applicability. In this study, we introduce a novel platform: DNA nanoribbon-templated copper nanoclusters (DNR/Cu NCs), which harness dual antioxidative mechanisms (direct ROS scavenging and activation of nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway) to synergistically mitigate oxidative stress. Unlike conventional nanozymes that rely on a single mechanism, DNR/Cu NCs exhibit combined superoxide dismutase (SOD)/catalase (CAT)/glutathione peroxidase (GPx), thereby enhancing overall ROS elimination. The biocompatible DNR scaffold facilitates the formation of ultrasmall Cu NCs with high catalytic activity and promotes NRF2 nuclear translocation to transcriptionally upregulate HO-1, amplifying endogenous antioxidant defenses. In both hepatocyte and zebrafish models of oxidative injury, the DNR/Cu NCs effectively suppressed ROS accumulation, suppressed apoptosis, and restored redox balance while mitigating tissue damage in vivo. This study highlights a paradigm-shifting approach in nanozyme design, offering a promising therapeutic avenue for oxidative stress-related diseases.
Employing antioxidant nanozymes to eliminate reactive oxygen species (ROS) is a promising strategy for alleviating oxidative stress. However, most current nanozymes struggle to balance catalytic efficacy with biosafety, limiting their clinical applicability. In this study, we introduce a novel platform: DNA nanoribbon-templated copper nanoclusters (DNR/Cu NCs), which harness dual antioxidative mechanisms (direct ROS scavenging and activation of nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway) to synergistically mitigate oxidative stress. Unlike conventional nanozymes that rely on a single mechanism, DNR/Cu NCs exhibit combined superoxide dismutase (SOD)/catalase (CAT)/glutathione peroxidase (GPx), thereby enhancing overall ROS elimination. The biocompatible DNR scaffold facilitates the formation of ultrasmall Cu NCs with high catalytic activity and promotes NRF2 nuclear translocation to transcriptionally upregulate HO-1, amplifying endogenous antioxidant defenses. In both hepatocyte and zebrafish models of oxidative injury, the DNR/Cu NCs effectively suppressed ROS accumulation, suppressed apoptosis, and restored redox balance while mitigating tissue damage in vivo. This study highlights a paradigm-shifting approach in nanozyme design, offering a promising therapeutic avenue for oxidative stress-related diseases.
关键词:
Construction land use efficiency;Evolution mechanism;Social network analysis;Spatial correlation network;China
摘要:
Efficient construction land use is essential to alleviate land scarcity and foster regional coordination, aligning with China's sustainability goals. Despite extensive research on construction land use efficiency (CLUE) dynamics, its potential spatial interactions across regions are not fully understood. This study applies social network analysis to investigate the evolution and driving mechanisms of China's spatial correlation network of CLUE from 2005 to 2019, based on a multi-temporal assessment at the provincial level. Results reveal that although most provinces achieved improvements in CLUE over the study period, pervasive underutilization and severe spatial disparities persist across regions. The inter-provincial linkages of CLUE, particularly in central and eastern China, have clearly strengthened, transforming four local spatial networks into a single, integrated nationwide network. Beijing, Shanghai, and Tianjin, owing to their relatively high efficiency and external influence, play pivotal roles within the evolving network. The spatial patterns are primarily linked to rapid yet uneven growth in population, gross domestic product (GDP), and fiscal investment, along with the basic farmland protection policies. By elucidating the complex and heterogeneous interprovincial spatial linkages of CLUE in China, this study underscores the critical role of optimized network structure in systemically enhancing CLUE, while offering practical guidance for enhancing interregional land governance coordination and advancing sustainable land use in China.
Efficient construction land use is essential to alleviate land scarcity and foster regional coordination, aligning with China's sustainability goals. Despite extensive research on construction land use efficiency (CLUE) dynamics, its potential spatial interactions across regions are not fully understood. This study applies social network analysis to investigate the evolution and driving mechanisms of China's spatial correlation network of CLUE from 2005 to 2019, based on a multi-temporal assessment at the provincial level. Results reveal that although most provinces achieved improvements in CLUE over the study period, pervasive underutilization and severe spatial disparities persist across regions. The inter-provincial linkages of CLUE, particularly in central and eastern China, have clearly strengthened, transforming four local spatial networks into a single, integrated nationwide network. Beijing, Shanghai, and Tianjin, owing to their relatively high efficiency and external influence, play pivotal roles within the evolving network. The spatial patterns are primarily linked to rapid yet uneven growth in population, gross domestic product (GDP), and fiscal investment, along with the basic farmland protection policies. By elucidating the complex and heterogeneous interprovincial spatial linkages of CLUE in China, this study underscores the critical role of optimized network structure in systemically enhancing CLUE, while offering practical guidance for enhancing interregional land governance coordination and advancing sustainable land use in China.
期刊:
Sensors and Actuators B-Chemical,2026年448:138928 ISSN:0925-4005
通讯作者:
Zhihui Wu<&wdkj&>Shi Gang Liu
作者机构:
[Zhihui Wu; Xiangzhi Li; Yucong Ma; Fangze Qin; Shi Gang Liu] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China;Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China;[Haoyang He; Hongbin Pu] School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China<&wdkj&>Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China<&wdkj&>Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China
通讯机构:
[Zhihui Wu; Shi Gang Liu] H;Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要:
Aflatoxin B1 (AFB1) contamination in foods threatens human health worldwide, and accurate and rapid detection of AFB1 is essential. Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-mode aptasensor is constructed for sensitive detection of AFB1 based on ZnFe 2 O 4 magnetic nanoparticle@gold nanoparticles-silver shell nanostructures (MNP@AuNPs-Ag) and fluorescent N-doped carbon dots (N-CDs). Specifically, Raman beacon molecule cyanine 3-modified aptamer (Apt-Cy3) is coupled on the MNP@AuNPs-Ag surface, and complementary DNA (cDNA) is modified on the N-CDs surface (N-CDs-cDNA). In the absence of AFB1, thanks to the DNA hybridization, low fluorescence for the supernatant and low SERS for the precipitate appear after magnetic separation. However, in the presence of AFB1, most of N-CDs-cDNA are free as the aptamer preferentially binds to AFB1, resulting in a strong fluorescent signal in the supernatant, while the change in the secondary structure of the aptamer shortens the spatial distance between Cy3 and MNP@AuNPs-Ag, producing a strong SERS signal. Based on the principle, the dual-mode aptasensor is developed and quantifies AFB1 using SERS and fluorescence signals with the low detection limits of 0.88 and 9.74 pg/mL, respectively. What's more, the dual-mode aptasensor is applied to the detection of AFB1 in real food samples successfully. This work exploits magnetic plasmonic core-shell nanostructures and fluorescent N-CDs to obtain fluorescence and SERS signals from the supernatant and the precipitate respectively after magnetic separation. The strategy not only provides a robust method for detection of AFB1 but also offers new insights for the design of dual-mode aptasensor.
Aflatoxin B1 (AFB1) contamination in foods threatens human health worldwide, and accurate and rapid detection of AFB1 is essential. Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-mode aptasensor is constructed for sensitive detection of AFB1 based on ZnFe 2 O 4 magnetic nanoparticle@gold nanoparticles-silver shell nanostructures (MNP@AuNPs-Ag) and fluorescent N-doped carbon dots (N-CDs). Specifically, Raman beacon molecule cyanine 3-modified aptamer (Apt-Cy3) is coupled on the MNP@AuNPs-Ag surface, and complementary DNA (cDNA) is modified on the N-CDs surface (N-CDs-cDNA). In the absence of AFB1, thanks to the DNA hybridization, low fluorescence for the supernatant and low SERS for the precipitate appear after magnetic separation. However, in the presence of AFB1, most of N-CDs-cDNA are free as the aptamer preferentially binds to AFB1, resulting in a strong fluorescent signal in the supernatant, while the change in the secondary structure of the aptamer shortens the spatial distance between Cy3 and MNP@AuNPs-Ag, producing a strong SERS signal. Based on the principle, the dual-mode aptasensor is developed and quantifies AFB1 using SERS and fluorescence signals with the low detection limits of 0.88 and 9.74 pg/mL, respectively. What's more, the dual-mode aptasensor is applied to the detection of AFB1 in real food samples successfully. This work exploits magnetic plasmonic core-shell nanostructures and fluorescent N-CDs to obtain fluorescence and SERS signals from the supernatant and the precipitate respectively after magnetic separation. The strategy not only provides a robust method for detection of AFB1 but also offers new insights for the design of dual-mode aptasensor.
作者机构:
[Haodong Zhang; Ning Su; Zhi Peng; Xizhe Luo; Sihai Qin; Mengjiao Huang; Jun Xie] College of Resources, Hunan Agricultural University, Changsha 410128, China;Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Changsha 410128, China;National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, China;[Gongwen Luo; Chang Tian; Xiangmin Rong; Guixian Xie] College of Resources, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Changsha 410128, China<&wdkj&>National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, China
通讯机构:
[Guixian Xie] C;College of Resources, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Changsha 410128, China<&wdkj&>National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, China
关键词:
N and P fertilizer;Rice-oilseed rape rotation;Soil properties;Runoff loss;Grain yield
摘要:
To address environmental pollution from agricultural non-point sources, it is feasible to reduce nitrogen (N) or phosphorus (P) inputs in farmland ecosystems. However, the combined effects of simultaneously reducing both N and P fertilizers remain unclear. Hence, we carried out a two-year field experiment (2020–2022) in the Dongting Lake region, China, to examine the impact of decreasing N and P fertilizer inputs on the soil properties, N and P runoff losses, and grain yields under rice-oilseed rape rotation in southern China. Compared to traditional management (rice, 210 kg N ha −1 and 105 kg P 2 O 5 ha −1 ; oilseed rape, 180 kg N ha −1 and 90 kg P 2 O 5 ha −1 ), the 10–30 % reduction in N and P inputs decreased total N runoff losses by 19.2–48.7 % (rice seasons; 3.12–7.92 kg ha −1 ) and 26.1–58.1 % (oilseed rape seasons; 1.17–2.61 kg ha −1 ), and total P losses by 35.5–59.7 % (rice seasons; 0.95–1.59 kg ha −1 ) and 30.3–58.5 % (oilseed rape seasons; 0.20–0.38 kg ha −1 ). Moreover, grain yields were maintained despite nutrient input reductions. The partial least square structural equation model showed that the soil total N, soil available N, and soil available P positively affected the grain yields, while the N and P runoff losses had a significant negative effect on the grain yields. In conclusion, the reduction of N and P fertilizer by 30 % achieved optimal outcomes, effectively reducing environmental risks while maintaining agricultural productivity.
To address environmental pollution from agricultural non-point sources, it is feasible to reduce nitrogen (N) or phosphorus (P) inputs in farmland ecosystems. However, the combined effects of simultaneously reducing both N and P fertilizers remain unclear. Hence, we carried out a two-year field experiment (2020–2022) in the Dongting Lake region, China, to examine the impact of decreasing N and P fertilizer inputs on the soil properties, N and P runoff losses, and grain yields under rice-oilseed rape rotation in southern China. Compared to traditional management (rice, 210 kg N ha −1 and 105 kg P 2 O 5 ha −1 ; oilseed rape, 180 kg N ha −1 and 90 kg P 2 O 5 ha −1 ), the 10–30 % reduction in N and P inputs decreased total N runoff losses by 19.2–48.7 % (rice seasons; 3.12–7.92 kg ha −1 ) and 26.1–58.1 % (oilseed rape seasons; 1.17–2.61 kg ha −1 ), and total P losses by 35.5–59.7 % (rice seasons; 0.95–1.59 kg ha −1 ) and 30.3–58.5 % (oilseed rape seasons; 0.20–0.38 kg ha −1 ). Moreover, grain yields were maintained despite nutrient input reductions. The partial least square structural equation model showed that the soil total N, soil available N, and soil available P positively affected the grain yields, while the N and P runoff losses had a significant negative effect on the grain yields. In conclusion, the reduction of N and P fertilizer by 30 % achieved optimal outcomes, effectively reducing environmental risks while maintaining agricultural productivity.
期刊:
Electric Power Systems Research,2026年252:112355 ISSN:0378-7796
通讯作者:
Bo Liu
作者机构:
[Borui Zhang; Bo Liu] College of Information and Intelligence, Hunan Agricultural University, Changsha 410128, China
通讯机构:
[Bo Liu] C;College of Information and Intelligence, Hunan Agricultural University, Changsha 410128, China
摘要:
As a representative uncertain renewable energy source, photovoltaic (PV) power generation is highly sensitive to meteorological conditions, posing significant challenges for microgrid operation and scheduling. To address the limitations of traditional forecasting methods in simultaneously achieving high prediction accuracy and robust uncertainty quantification, this study proposes a multi-quantile forecasting model integrating a Bidirectional Temporal Convolutional Network (BiTCN), Efficient Channel Attention (ECA), and Quantile Regression (QR). The model unifies point and interval forecasting within a single framework, enhancing interval coverage and width control via an optimized quantile loss function. Experiments on real-world PV data from a Chinese power station demonstrate robust performance across sunny, cloudy, and rainy conditions.Compared with the best-performing baseline (BiLSTM+Attention), the proposed approach reduces MAE by 10.8% and RMSE by 5.2%. Besides,the model achieves a Prediction Interval Coverage Probability (PICP) exceeding 85% at an 80% confidence level, and maintains a Prediction Interval Normalized Average Width (PINAW) below 25%.
As a representative uncertain renewable energy source, photovoltaic (PV) power generation is highly sensitive to meteorological conditions, posing significant challenges for microgrid operation and scheduling. To address the limitations of traditional forecasting methods in simultaneously achieving high prediction accuracy and robust uncertainty quantification, this study proposes a multi-quantile forecasting model integrating a Bidirectional Temporal Convolutional Network (BiTCN), Efficient Channel Attention (ECA), and Quantile Regression (QR). The model unifies point and interval forecasting within a single framework, enhancing interval coverage and width control via an optimized quantile loss function. Experiments on real-world PV data from a Chinese power station demonstrate robust performance across sunny, cloudy, and rainy conditions.Compared with the best-performing baseline (BiLSTM+Attention), the proposed approach reduces MAE by 10.8% and RMSE by 5.2%. Besides,the model achieves a Prediction Interval Coverage Probability (PICP) exceeding 85% at an 80% confidence level, and maintains a Prediction Interval Normalized Average Width (PINAW) below 25%.
期刊:
Journal of Hydrology,2026年664:134412 ISSN:0022-1694
通讯作者:
Zhenwei Li<&wdkj&>Liangxia Duan
作者机构:
[Yiting Hong; Haoyang Xin; Fengjiao Pan; Hongxia Xie; Liangxia Duan] College of Resources, Hunan Agricultural University, Changsha 410128, China;[Zhenwei Li] Guangxi Key Laboratory of Karst Ecological Processes and Services, Huanjiang Observation and Research Station for Karst Ecosystem, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
通讯机构:
[Zhenwei Li] G;[Liangxia Duan] C;Guangxi Key Laboratory of Karst Ecological Processes and Services, Huanjiang Observation and Research Station for Karst Ecosystem, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China<&wdkj&>College of Resources, Hunan Agricultural University, Changsha 410128, China
摘要:
As a key factor in shaping channel morphology, effective discharge ( Q e ) can quantitatively link water discharge to geomorphic processes. However, a better understanding of Q e estimation and its geomorphic impacts is still challenged by the diversity of values identified for various catchments. To address this issue, the objectives of this study were to calculate Q e using both analytical and class-based approaches in 8 karst catchments during 2003–2023 based on monthly water discharge and sediment load, and further to evaluate the effect of Q e on channel adjustment. Results showed that water discharge and sediment load in each catchment exhibited significant seasonal variations, and annual sediment load was generally governed by the period from June to September. The Q e obtained by two methods differed considerably. The recurrence interval of Q e ranged from 1.37 to 4.07 and 1.04 to 10.15 years obtained by the analytical and class-based approaches, respectively. The Q e was also closely related to sediment transport and deposition processes, and thus the channel morphology. Specifically, the proportion of discharge required to transport 50 % of the suspended sediment ranged from 20 % to 67 %, while the proportion of duration required for this transport ranged from 22 % to 69 %. The decreased Q e could cause channel aggradation, while increased Q e led to channel incision. This study can provide a new perspective for better understanding the linkage between hydrology and channel morphology, and offer valuable information for effectively controlling sediment delivery in karst catchments.
As a key factor in shaping channel morphology, effective discharge ( Q e ) can quantitatively link water discharge to geomorphic processes. However, a better understanding of Q e estimation and its geomorphic impacts is still challenged by the diversity of values identified for various catchments. To address this issue, the objectives of this study were to calculate Q e using both analytical and class-based approaches in 8 karst catchments during 2003–2023 based on monthly water discharge and sediment load, and further to evaluate the effect of Q e on channel adjustment. Results showed that water discharge and sediment load in each catchment exhibited significant seasonal variations, and annual sediment load was generally governed by the period from June to September. The Q e obtained by two methods differed considerably. The recurrence interval of Q e ranged from 1.37 to 4.07 and 1.04 to 10.15 years obtained by the analytical and class-based approaches, respectively. The Q e was also closely related to sediment transport and deposition processes, and thus the channel morphology. Specifically, the proportion of discharge required to transport 50 % of the suspended sediment ranged from 20 % to 67 %, while the proportion of duration required for this transport ranged from 22 % to 69 %. The decreased Q e could cause channel aggradation, while increased Q e led to channel incision. This study can provide a new perspective for better understanding the linkage between hydrology and channel morphology, and offer valuable information for effectively controlling sediment delivery in karst catchments.
作者机构:
[Lingqiong Song; Zhihui Liu; Qiyuan Tang; Min Huang; Jiana Chen; Weiqing Wang; Huabin Zheng] College of Agronomy, Hunan agricultural University, Changsha 410128, China;[Yuanwei Chen] Crop Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China
通讯机构:
[Weiqing Wang; Huabin Zheng] C;College of Agronomy, Hunan agricultural University, Changsha 410128, China
关键词:
Carbon pool management index;Grain yield;Labile organic carbon;Ratoon rice
摘要:
Integrated crop and soil management measures have proven effective in enhancing crop yields and resource use efficiency, although the impacts on soil carbon pools and related microbial diversity remain unclear. In this study, we compared rice yield and soil properties between a local ratoon rice farming practice model without organic fertilizer application (FP) with an improved farming practice model (IFP) based on integrated management practices, including a > 20 % increase in planting density, 30 % increase in panicle fertilization, and application of 1.8 t/ha of organic fertilizer, in Hunan Province, China. The 5-year average annual rice grain yield was 32.0 % higher in the IFP (13.3 t/ha) than in the FP. Soil organic carbon (SOC) content was nonsignificantly higher by 9.2 % in the IFP (22.4 g/kg) than in the FP; furthermore, the labile organic carbon and dissolved organic carbon contents were 10.7 g/kg and 45.5 mg/kg, respectively, in the IFP, significantly higher than those of the FP and N-free control. The carbon pool management index in the IFP was 191.4 in 2022 and 132.9 in 2023, and was significantly higher by 95.0 % ( P < 0.05) than in the FP; the carbon pool activity and carbon pool activity index were similarly improved in the IFP. The average microbial biomass carbon in the IFP was 179.3 mg/kg, but did not differ significantly among the IFP, FP, and control. The bacterial and fungal Chao1 indexes were 5.6 % and 13.3 % higher, respectively, in the IFP (Chao1 for bacteria: 5252.5; fungi: 2291.5) than in the FP. The bacterial and fungal abundance-based coverage estimator (ACE) indexes were 7.2 % and 13.3 % higher, respectively, in the IFP (ACE for bacteria: 5871.5; fungi: 2530.4) than in the FP; however, there was no significant difference between the IFP and FP ( P > 0.05). These results support the efficacy of integrated agronomic measures in greatly increasing rice grain yield while supporting soil fertility through enhancing carbon pools and related microbial diversity. As modern ratoon rice production becomes increasingly mechanized, such measures will become easy to adopt.
Integrated crop and soil management measures have proven effective in enhancing crop yields and resource use efficiency, although the impacts on soil carbon pools and related microbial diversity remain unclear. In this study, we compared rice yield and soil properties between a local ratoon rice farming practice model without organic fertilizer application (FP) with an improved farming practice model (IFP) based on integrated management practices, including a > 20 % increase in planting density, 30 % increase in panicle fertilization, and application of 1.8 t/ha of organic fertilizer, in Hunan Province, China. The 5-year average annual rice grain yield was 32.0 % higher in the IFP (13.3 t/ha) than in the FP. Soil organic carbon (SOC) content was nonsignificantly higher by 9.2 % in the IFP (22.4 g/kg) than in the FP; furthermore, the labile organic carbon and dissolved organic carbon contents were 10.7 g/kg and 45.5 mg/kg, respectively, in the IFP, significantly higher than those of the FP and N-free control. The carbon pool management index in the IFP was 191.4 in 2022 and 132.9 in 2023, and was significantly higher by 95.0 % ( P < 0.05) than in the FP; the carbon pool activity and carbon pool activity index were similarly improved in the IFP. The average microbial biomass carbon in the IFP was 179.3 mg/kg, but did not differ significantly among the IFP, FP, and control. The bacterial and fungal Chao1 indexes were 5.6 % and 13.3 % higher, respectively, in the IFP (Chao1 for bacteria: 5252.5; fungi: 2291.5) than in the FP. The bacterial and fungal abundance-based coverage estimator (ACE) indexes were 7.2 % and 13.3 % higher, respectively, in the IFP (ACE for bacteria: 5871.5; fungi: 2530.4) than in the FP; however, there was no significant difference between the IFP and FP ( P > 0.05). These results support the efficacy of integrated agronomic measures in greatly increasing rice grain yield while supporting soil fertility through enhancing carbon pools and related microbial diversity. As modern ratoon rice production becomes increasingly mechanized, such measures will become easy to adopt.
作者:
Ding, Bonan;Liu, Jiaqi;Kasay, Igor Lumbeto Tenda;Konsue, Nattaya;Lin, Xuan;...
期刊:
Journal of Ethnopharmacology,2026年354:120519 ISSN:0378-8741
通讯作者:
Lv, Chenghao;Xia, Hongjuan;Qin, Si
作者机构:
[Kasay, Igor Lumbeto Tenda; Liu, Jiaqi; Qin, Si; Yan, Fangfang; Ding, Bonan] Lab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Konsue, Nattaya] Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai, 57100, Thailand;[Lin, Xuan] Department of Endocrinology, CR & WISCO General Hospital, Wuhan University of Science and Technology, Wuhan, 430080, China;[Wang, Dongsheng] Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China;[Lv, Chenghao] Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address: lvchenghao@xiangya.com.cn
通讯机构:
[Xia, Hongjuan] L;[Lv, Chenghao; Qin, Si] I;Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address:;Lab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China. Electronic address:;Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address:
摘要:
Ethnopharmacological relevance Corus officinalis Siebold & Zucc belongs to the genus Cornus in the Cornaceae family, and was first recorded in the “Shennong Herbal Classic”, now has been included in “according to the tradition of both food and Chinese herbal medicines”, consist of kidney and liver tonifying, antioxidant substances including cycloid glycosides, flavonoids, polyphenols, organic acids, etc.
Corus officinalis Siebold & Zucc belongs to the genus Cornus in the Cornaceae family, and was first recorded in the “Shennong Herbal Classic”, now has been included in “according to the tradition of both food and Chinese herbal medicines”, consist of kidney and liver tonifying, antioxidant substances including cycloid glycosides, flavonoids, polyphenols, organic acids, etc.
Aim of the study This study was aimed at discovering the mechanism underlying the anti-hyperemia effect of Cor in rats, particularly its protective effect against liver and kidney dysfunction caused by HUA.
This study was aimed at discovering the mechanism underlying the anti-hyperemia effect of Cor in rats, particularly its protective effect against liver and kidney dysfunction caused by HUA.
Materials and methods In this study, the effect of Cor extract against HUA was verified in rats, subsequently, network pharmacology combined with non-targeted metabolomic were performed to investigate its composition characteristics, and further multi-omics studies and molecular validation were performed to reveal molecular mechanism both in vivo and in vitro .
In this study, the effect of Cor extract against HUA was verified in rats, subsequently, network pharmacology combined with non-targeted metabolomic were performed to investigate its composition characteristics, and further multi-omics studies and molecular validation were performed to reveal molecular mechanism both in vivo and in vitro .
Results The results demonstrated that cornuside, hydroxygenkwanin and tetrahydroalstonine were the main bioactive compounds in Cor extract, which protected intestinal metabolism disorder by increasing relative abundances of Bacteroides, Lactobacillus Roseburia and Akkermansia , alleviated liver oxidative damage by activation of the Nrf2/HO-1(NQO1) antioxidant pathway, and reduced liver UA synthesis by inhibiting the expression of UA synthesis protein XOD in rat model. In addition, tetrahydroalstonine alleviated inflammation via inhibiting PI3K/Akt/NF-κB signaling pathway, with cornuside and hydroxygenkwanin enhanced renal tubule UA transport capacity by regulating the translations of XDH and HRP genes, all of which protected HUA-rat kidney from inflammatory infiltration damage, and reduced serum urea nitrogen (BUN), creatinine (CRE) and UA levels.
The results demonstrated that cornuside, hydroxygenkwanin and tetrahydroalstonine were the main bioactive compounds in Cor extract, which protected intestinal metabolism disorder by increasing relative abundances of Bacteroides, Lactobacillus Roseburia and Akkermansia , alleviated liver oxidative damage by activation of the Nrf2/HO-1(NQO1) antioxidant pathway, and reduced liver UA synthesis by inhibiting the expression of UA synthesis protein XOD in rat model. In addition, tetrahydroalstonine alleviated inflammation via inhibiting PI3K/Akt/NF-κB signaling pathway, with cornuside and hydroxygenkwanin enhanced renal tubule UA transport capacity by regulating the translations of XDH and HRP genes, all of which protected HUA-rat kidney from inflammatory infiltration damage, and reduced serum urea nitrogen (BUN), creatinine (CRE) and UA levels.
Conclusions These findings indicates that Cor can alleviate HUA by enhancing liver-renal-intestine UA metabolism, inhibiting inflammatory responses of liver-renal-intestine as well as providing hepatorenal protection.
These findings indicates that Cor can alleviate HUA by enhancing liver-renal-intestine UA metabolism, inhibiting inflammatory responses of liver-renal-intestine as well as providing hepatorenal protection.
作者机构:
[Yi Wang; Pengjie Wang; Peipei Wu] Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China;[Jinghua Yu] College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, 300457, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China;Mengniu Hi-Tech Dairy Products (Beijing) Co., Ltd, Beijing, 101100, China;[Menghui Wang; Jinhui Yang] Inner Mongolia Mengniu Dairy (Group) Co., Ltd, Hohhot, 011500, China
通讯机构:
[Jinghua Yu] C;College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, 300457, China
摘要:
Creaming is an issue in UHT milk due to extended storage periods and higher storage temperatures. Therefore, this study aims to unravel the key interfacial molecules and core structure behind the stability of UHT milk fat globules . Raw milk from Holstein, Jersey, and goat breeds, which differ in interfacial components, was selected. The fat globules were standardized for particle size using the UHT system, and stability analysis was performed using Turbiscan to establish high, medium, and low stability fat globule models. Omics analysis revealed that the abundance of 3 interfacial proteins (butyrophilin, adipophilin, and xanthine oxidoreductase) and 2 interfacial phospholipids (phosphatidylserine and sphingomyelin) significantly increased as the fat globule stability increased. Support vector machine was used to identify key interfacial molecules. The results showed that the high abundance of butyrophilin, adipophilin, phosphatidylserine , and sphingomyelin contributed to fat globule stability. Molecular docking and CHARMM-GUI further determined that butyrophilin interacted with adipophilin mainly through hydrogen bonds (ΔG = −47.8 kcal/mol and interaction area = 3088.4 Å 2 ). The abundance of this complex in high-stability fat globules was 2.2 times greater than that in medium-stability fat globules and 4.9 times greater than that in low-stability fat globules, respectively. We hypothesize that the butyrophilin-adipophilin complex may anchor both the inner monolayer and the outer bilayer, forming the backbone of the entire interface. Therefore, this study first reveals that the key molecules contributing to the stability of UHT milk fat globules are butyrophilin, adipophilin, phosphatidylserine, and sphingomyelin, with the butyrophilin-adipophilin complex serving as the core structure.
Creaming is an issue in UHT milk due to extended storage periods and higher storage temperatures. Therefore, this study aims to unravel the key interfacial molecules and core structure behind the stability of UHT milk fat globules . Raw milk from Holstein, Jersey, and goat breeds, which differ in interfacial components, was selected. The fat globules were standardized for particle size using the UHT system, and stability analysis was performed using Turbiscan to establish high, medium, and low stability fat globule models. Omics analysis revealed that the abundance of 3 interfacial proteins (butyrophilin, adipophilin, and xanthine oxidoreductase) and 2 interfacial phospholipids (phosphatidylserine and sphingomyelin) significantly increased as the fat globule stability increased. Support vector machine was used to identify key interfacial molecules. The results showed that the high abundance of butyrophilin, adipophilin, phosphatidylserine , and sphingomyelin contributed to fat globule stability. Molecular docking and CHARMM-GUI further determined that butyrophilin interacted with adipophilin mainly through hydrogen bonds (ΔG = −47.8 kcal/mol and interaction area = 3088.4 Å 2 ). The abundance of this complex in high-stability fat globules was 2.2 times greater than that in medium-stability fat globules and 4.9 times greater than that in low-stability fat globules, respectively. We hypothesize that the butyrophilin-adipophilin complex may anchor both the inner monolayer and the outer bilayer, forming the backbone of the entire interface. Therefore, this study first reveals that the key molecules contributing to the stability of UHT milk fat globules are butyrophilin, adipophilin, phosphatidylserine, and sphingomyelin, with the butyrophilin-adipophilin complex serving as the core structure.
作者机构:
[Quan Zhang; Bo Li; Qihong Zhu; Daoyou Huang; Chao Xu; Hanhua Zhu] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;University of Chinese Academy of Sciences, Beijing 100049, China;[Haifei Chen; Qiren Wen] School of Resources and Environment, Hunan Agricultural University, Changsha 410125, China;[Tianyi Yan; Jingheng Zhou] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China<&wdkj&>University of Chinese Academy of Sciences, Beijing 100049, China
通讯机构:
[Quan Zhang; Hanhua Zhu] I;Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
摘要:
Manganese (Mn) is widely used to control cadmium (Cd) and arsenic (As) uptake by rice, but the effects of different Mn forms and concentrations on Cd/As movement in the soil-rice system are unclear. The study investigated the mechanisms by which three Mn compounds affected the accumulation of Cd/As in rice under different application rates. MnO 2 , MnSO 4 , and MnCO 3 treatments significantly reduced grains Cd levels by 27.6 %, 30.2 %, and 28.1 %, respectively, while As levels were less consistently affected. Three forms of Mn fertilizers enhanced the conversion of exchangeable to carbonate bound-Cd, which closely related to the increase of soil pH. MnO 2 and MnCO 3 reduced Cd translocation by increasing Cd/As adsorption on iron plaques, and MnSO 4 and MnCO 3 decreased Cd translocation by boosting root SOD and Cys levels. Transcriptome analysis revealed that Mn 2+ upregulated genes involved in the antioxidant defense system, limited Cd transport by enhancing OsABCC1 and OsHMA3 expression, and promoted As translocation by increasing OsLsi2 expression. Overall, different forms of Mn fertilizers effectively reduced Cd toxicity by fixing Cd in soil carbonate and iron plaques, and restricting Cd transport. Although Mn fertilizers reduced As availability in soil and affected As absorption in rice, they have certain limitations and need to be further explored. These findings reveal the mechanism by which different forms of Mn regulate the fixation and migration behavior of Cd and As, providing new ideas and theoretical basis for reducing the environmental risk of Cd and As.
Manganese (Mn) is widely used to control cadmium (Cd) and arsenic (As) uptake by rice, but the effects of different Mn forms and concentrations on Cd/As movement in the soil-rice system are unclear. The study investigated the mechanisms by which three Mn compounds affected the accumulation of Cd/As in rice under different application rates. MnO 2 , MnSO 4 , and MnCO 3 treatments significantly reduced grains Cd levels by 27.6 %, 30.2 %, and 28.1 %, respectively, while As levels were less consistently affected. Three forms of Mn fertilizers enhanced the conversion of exchangeable to carbonate bound-Cd, which closely related to the increase of soil pH. MnO 2 and MnCO 3 reduced Cd translocation by increasing Cd/As adsorption on iron plaques, and MnSO 4 and MnCO 3 decreased Cd translocation by boosting root SOD and Cys levels. Transcriptome analysis revealed that Mn 2+ upregulated genes involved in the antioxidant defense system, limited Cd transport by enhancing OsABCC1 and OsHMA3 expression, and promoted As translocation by increasing OsLsi2 expression. Overall, different forms of Mn fertilizers effectively reduced Cd toxicity by fixing Cd in soil carbonate and iron plaques, and restricting Cd transport. Although Mn fertilizers reduced As availability in soil and affected As absorption in rice, they have certain limitations and need to be further explored. These findings reveal the mechanism by which different forms of Mn regulate the fixation and migration behavior of Cd and As, providing new ideas and theoretical basis for reducing the environmental risk of Cd and As.
摘要:
To investigate whether patterns of ploidy state variation known to occur in macrophytes at broad global scales can be detected at finer site scale, we examined macrophyte assemblages present in 1239 individual inland lentic and lotic waterbodies sampled from 2000 onwards. The sites include lakes and reservoirs, rivers and streams, slow-flowing or static water bodies associated with rivers (such as oxbows), man-made channels, and temporary or ephemeral lentic waterbodies in 22 countries worldwide. The latitude range for these sites was 10.58–68.40° N and from 0.01 to 54.88° S, covering climatic conditions ranging from tropical to temperate/Arctic. We examined the influence of geospatial variables, current or historic climate variables, and additional local water physical and chemical variables measured for each site, as potential predictors of the incidence of ploidy state (diploidy, polyploidy, and mixed-cytotype) in the macrophyte species assemblage. At fine scales (individual sites), we observed the same latitudinal and climatic patterns influencing all macrophyte ploidy states, especially diploid species, compared to findings at a broad spatial resolution of 10° × 10° latitude-longitude. Ploidy state of macrophyte assemblages slightly, but significantly, differs between lentic and lotic environments. Along with geospatial and climate variables, local physical and chemical variables also helped predict the occurrence of polyploid and mixed-ploidy species. Our results support previous findings on ploidy state distribution and drivers at broader scales but also unravel new information on key drivers for the distribution of polyploid and mixed-ploidy species, relevant to understanding macrophyte adaptation mechanisms and evolutionary processes worldwide.
To investigate whether patterns of ploidy state variation known to occur in macrophytes at broad global scales can be detected at finer site scale, we examined macrophyte assemblages present in 1239 individual inland lentic and lotic waterbodies sampled from 2000 onwards. The sites include lakes and reservoirs, rivers and streams, slow-flowing or static water bodies associated with rivers (such as oxbows), man-made channels, and temporary or ephemeral lentic waterbodies in 22 countries worldwide. The latitude range for these sites was 10.58–68.40° N and from 0.01 to 54.88° S, covering climatic conditions ranging from tropical to temperate/Arctic. We examined the influence of geospatial variables, current or historic climate variables, and additional local water physical and chemical variables measured for each site, as potential predictors of the incidence of ploidy state (diploidy, polyploidy, and mixed-cytotype) in the macrophyte species assemblage. At fine scales (individual sites), we observed the same latitudinal and climatic patterns influencing all macrophyte ploidy states, especially diploid species, compared to findings at a broad spatial resolution of 10° × 10° latitude-longitude. Ploidy state of macrophyte assemblages slightly, but significantly, differs between lentic and lotic environments. Along with geospatial and climate variables, local physical and chemical variables also helped predict the occurrence of polyploid and mixed-ploidy species. Our results support previous findings on ploidy state distribution and drivers at broader scales but also unravel new information on key drivers for the distribution of polyploid and mixed-ploidy species, relevant to understanding macrophyte adaptation mechanisms and evolutionary processes worldwide.
摘要:
Electrocatalytic nitrate reduction reaction (NO(3)RR) represents a sustainable and environmentally benign route for ammonia (NH(3)) synthesis. However, NO(3)RR is still limited by the competition from hydrogen evolution reaction (HER) and the high energy barrier in the hydrogenation step of nitrogen-containing intermediates. Here, we report a selective etching strategy to construct RuM nanoalloys (M = Fe, Co, Ni, Cu) uniformly dispersed on porous nitrogen-doped carbon substrates for efficient neutral NH(3) electrosynthesis. Density functional theory calculations confirm that the synergic effect between Ru and transition metal M modulates the electronic structure of the alloy, significantly lowering the energy barrier for the conversion of *NO(2) to *HNO(2). Experimentally, the optimized RuFe-NC catalyst achieves 100% Faraday efficiency with a high yield rate of 0.83mgh(-1) mg(cat)(-1) at a low potential of - 0.1V vs. RHE, outperforming most reported catalysts. In situ spectroscopic analyses further demonstrate that the RuM-NC effectively promotes the hydrogenation of nitrogen intermediates while inhibiting the formation of hydrogen radicals, thereby reducing HER competition. The RuFe-NC assembled Zn-NO(3)(-) battery achieved a high open-circuit voltage and an outstanding power density and capacity, which drive selective NO(3)(-) conversion to NH(3). This work provides a powerful synergistic design strategy for efficient NH(3) electrosynthesis and a general framework for the development of advanced multi-component catalysts for sustainable nitrogen conversion.
期刊:
Postharvest Biology and Technology,2026年232:113965 ISSN:0925-5214
通讯作者:
Chen Zhang
作者机构:
[Xiaoyu Tan; Quanzhi Wang; Enmei Hu] School of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang, Jiangsu 212400, China;National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China;[Yuhua Xie] School of Food and Biological Engineering, Hezhou University, Hezhou, Guangxi 542899, China;[Xuehui Li] College of Life Science, Nanyang Normal University, Nanyang, Henan 473061, China
通讯机构:
[Chen Zhang] C;College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
摘要:
Chilling exposure of tomato, especially at early ripening stage, would impact flavor quality in the red fruit; nevertheless, the molecular mechanism beneath such phenomenon has not been fully clarified until recently. In this study, ethylene evolution and thus ripening process of ‘FL 47’ tomato was suppressed after 5 ℃ exposure at mature green stage for 4 d. And the suppressed ethylene abundance was associated with the downregulated gene ( SlACO1 , SlACS2 / 4 , SlETR3/4/7 , SlEIN2 , SlEIL1 , SlEBF1/2 , and SlERF1 ) expression profiles, enzyme (ACO and ACS) activities, and ACC production in its biosynthesis & signaling transduction pathways. Additionally, the formation of TSS, glucose, fructose, and 13 key volatiles were suppressed by low-temperature treatment, while sucrose, TA, malate, citrate, and MeSA were enhanced, causing the negative impact on flavor profile. And the these quality changes were associated with the alternations of substrate (linoleic and linolenic acids, phytoene, phytofluene, trans -lycopene, γ-carotenoid, β-carotenoid, (iso)leucine, phenylalanine, salicylate (SA)) production, enzyme (SPS, A/NI, VI, PEPCK, IDH, NADP-ME, NADP-MDH, PEPC, LOX, HPL, PAL) activities, and gene ( SlSPSA2 / B / C , SlSUS4 /6, SlA/NI1 , SlVI , SlVIF , SlIDH3 , SlNADP-MDH , SlPEPC3 , SlNADP-ME2 , SlPEPCK1 , SlLOXC , Sl13-HPL , SlPSY1 , SlCCD1A/B , SlBCAT1 , SlAADC1A / B , SlAADC2 , SlPAR1/2 , SlICS , SlPAL4 , SlSAMT1 ) expression profiles in their metabolic pathways. Further study explored that ethylene and SAs played an antagonistic role in fruit ripening and then flavor metabolism. Taken together, our study implied that chilling exposure at mature green stage impacted flavor quality in the red fruit via regulating the crosstalk between ethylene and SAs metabolism.
Chilling exposure of tomato, especially at early ripening stage, would impact flavor quality in the red fruit; nevertheless, the molecular mechanism beneath such phenomenon has not been fully clarified until recently. In this study, ethylene evolution and thus ripening process of ‘FL 47’ tomato was suppressed after 5 ℃ exposure at mature green stage for 4 d. And the suppressed ethylene abundance was associated with the downregulated gene ( SlACO1 , SlACS2 / 4 , SlETR3/4/7 , SlEIN2 , SlEIL1 , SlEBF1/2 , and SlERF1 ) expression profiles, enzyme (ACO and ACS) activities, and ACC production in its biosynthesis & signaling transduction pathways. Additionally, the formation of TSS, glucose, fructose, and 13 key volatiles were suppressed by low-temperature treatment, while sucrose, TA, malate, citrate, and MeSA were enhanced, causing the negative impact on flavor profile. And the these quality changes were associated with the alternations of substrate (linoleic and linolenic acids, phytoene, phytofluene, trans -lycopene, γ-carotenoid, β-carotenoid, (iso)leucine, phenylalanine, salicylate (SA)) production, enzyme (SPS, A/NI, VI, PEPCK, IDH, NADP-ME, NADP-MDH, PEPC, LOX, HPL, PAL) activities, and gene ( SlSPSA2 / B / C , SlSUS4 /6, SlA/NI1 , SlVI , SlVIF , SlIDH3 , SlNADP-MDH , SlPEPC3 , SlNADP-ME2 , SlPEPCK1 , SlLOXC , Sl13-HPL , SlPSY1 , SlCCD1A/B , SlBCAT1 , SlAADC1A / B , SlAADC2 , SlPAR1/2 , SlICS , SlPAL4 , SlSAMT1 ) expression profiles in their metabolic pathways. Further study explored that ethylene and SAs played an antagonistic role in fruit ripening and then flavor metabolism. Taken together, our study implied that chilling exposure at mature green stage impacted flavor quality in the red fruit via regulating the crosstalk between ethylene and SAs metabolism.
期刊:
Water Research,2026年288(Pt B):124758 ISSN:0043-1354
通讯作者:
Liao, Peng;Liao, Wenjuan
作者机构:
[Cui, Haojie; Liao, Wenjuan; Peng, Wei; Wei, Minli] Yuelushan Laboratory, College of Resources, Hunan Agricultural University, Changsha, 410128, China;[Peng, Wei] State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, PR China;[Yuan, Songhu] State Key Laboratory of Geomicrobiology and Environmental Changes, China University of Geosciences, NO. 68 Jincheng Street, East Lake High-Tech Development Zone, Wuhan, 430074, PR China;[Liao, Peng] State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, PR China. Electronic address: liaopeng@mail.gyig.ac.cn;[Zhang, Xingming] School of Chemistry and Materials of Science, Hunan Agricultural University, Changsha, 410128, China
通讯机构:
[Peng Liao] S;[Wenjuan Liao] Y;State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, PR China<&wdkj&>Yuelushan Laboratory, College of Resources, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, PR China
摘要:
Interactions between iron (Fe) mineral colloids and phytic acid (myo-inositol hexaphosphate, IHP)—a widely distributed organic phosphorus compound—can strongly influence cycling of carbon and coupled elements in aquatic systems. While mechanisms of IHP interacting with Fe(III) minerals are understood, its role in modulating Fe(II)-sulfide (FeS) oxygenation and hydroxyl radical (•OH) generation at anoxic-oxic interfaces remains unresolved. Here, we demonstrate that IHP significantly enhances •OH production during FeS oxygenation at neutral pH in a concentration-dependent manner. Density functional theory calculations reveal that IHP preferentially coordinates with Top-Fe compared to Top-S sites on FeS surfaces, forming Fe(II)-IHP complexes that suppress structural Fe(II) oxidation. This shifts the oxygen reduction pathway from a two-electron to sequential single-electron pathway, increasing •OH yield by 2.0–8.9 fold. Spectroscopic and microscopic analyses show that IHP favors the formation of nanosized ferrihydrite over crystalline lepidocrocite, further enhancing •OH production. Additionally, IHP facilitates the release of surface-associated •OH into the aqueous phase, increasing availability to react with bulk constituents. Mineralization experiments show that 250 μM IHP enables FeS oxygenation to mineralize 7.4–41.9 % of dissolved organic matter (DOM, 1–15 mg C/L) to CO 2 , while no mineralization occurs in IHP-free systems. Taken together, our results unveil previously unrecognized mechanisms by which IHP enhances •OH production during FeS oxygenation and provide new insights into organic carbon dynamics in IHP(-FeS) rich, redox-dynamic aquatic environments.
Interactions between iron (Fe) mineral colloids and phytic acid (myo-inositol hexaphosphate, IHP)—a widely distributed organic phosphorus compound—can strongly influence cycling of carbon and coupled elements in aquatic systems. While mechanisms of IHP interacting with Fe(III) minerals are understood, its role in modulating Fe(II)-sulfide (FeS) oxygenation and hydroxyl radical (•OH) generation at anoxic-oxic interfaces remains unresolved. Here, we demonstrate that IHP significantly enhances •OH production during FeS oxygenation at neutral pH in a concentration-dependent manner. Density functional theory calculations reveal that IHP preferentially coordinates with Top-Fe compared to Top-S sites on FeS surfaces, forming Fe(II)-IHP complexes that suppress structural Fe(II) oxidation. This shifts the oxygen reduction pathway from a two-electron to sequential single-electron pathway, increasing •OH yield by 2.0–8.9 fold. Spectroscopic and microscopic analyses show that IHP favors the formation of nanosized ferrihydrite over crystalline lepidocrocite, further enhancing •OH production. Additionally, IHP facilitates the release of surface-associated •OH into the aqueous phase, increasing availability to react with bulk constituents. Mineralization experiments show that 250 μM IHP enables FeS oxygenation to mineralize 7.4–41.9 % of dissolved organic matter (DOM, 1–15 mg C/L) to CO 2 , while no mineralization occurs in IHP-free systems. Taken together, our results unveil previously unrecognized mechanisms by which IHP enhances •OH production during FeS oxygenation and provide new insights into organic carbon dynamics in IHP(-FeS) rich, redox-dynamic aquatic environments.
摘要:
In the context of high-quality agricultural development, farmers increasingly engage in agricultural land use rights transfer(ALURT) to achieve large-scale operations and improve agricultural production efficiency. However, large-scale agricultural operations often lead to mechanized production, which may cause higher carbon emissions, contradicting the principles of green agricultural development. This study aimed to assess the actual impact of ALURT on the carbon emission intensity (CEI) of croplands and explore the role of agricultural large-scale operations in this relationship. To achieve this, the CEI of arable land across 30 provinces in China from 2014 to 2022 was measured, and the impact of ALURT on the CEI was analyzed using a two-way fixed-effects model, a mediated-effects model, and a threshold-effects model. These findings suggested that the total carbon emissions and CEI of arable land in China have declined annually since 2015. Southeastern coastal provinces, including Shanghai and Zhejiang, have the highest CEI of croplands. ALURT significantly reduced the CEI for arable land. Moreover, mechanism testing revealed that large-scale operations did not have a mediating effect but instead exhibited a threshold effect. When the scale of agricultural operations grew to the threshold, the inhibition of ALURT on CEI could be amplified.
通讯机构:
[Li, W ] H;Hunan Acad Agr Sci, Tea Res Inst, Changsha 410125, Peoples R China.;Minist Agr, Hunan Tea Plant & Tea Proc Sci Observat Expt Stn, Changsha 410125, Peoples R China.;Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.
摘要:
Theanine is a crucial indicator of tea quality, and its significance is closely tied to the economic value of tea. There have been many reports on the regulation mechanism of theanine synthesis and accumulation, but the mechanism by which gibberellin regulates theanine synthesis in tea plants is poorly understood. Previous studies have shown that the content of theanine experiences significant changes in the growth stages of tea shoots, displaying a strong correlation with gibberellin. This study confirmed that gibberellin significantly promoted the expression of the major gene of theanine synthesis, known as CsTSI. Additionally, the study identified CsWRKY71 as a transcription factor that mediated the regulation by gibberellin of theanine synthesis in tea plants. CsWRKY71 was localized in the nucleus and had a typical WRKY domain. It was a member of subclass IIC and its expression was significantly suppressed following exogenous GA3 treatment. Further assays, such as the electrophoretic mobility shift assay, dual luciferase and asODN (antisense oligodeoxynucleotide) interference, demonstrated that CsWRKY71 significantly interacted with the promoter of CsTSI, which inhibited theanine synthesis by binding to the cis-acting element (C/T)TGAC(T/C) of the CsTSI promoter. Overall, the addition of exogenous gibberellin alleviated the inhibition of CsTSI by down-regulating the expression of CsWRKY71, ultimately facilitating the rapid biosynthesis of theanine. This study elucidated the molecular mechanism of CsWRKY71-mediated gibberellin regulation of theanine synthesis in tea plant. The findings not only enhance our understanding of the regulatory processes involved in theanine synthesis in tea plants, but also provide important references for maintaining the characteristics of high theanine in the tea plant.
关键词:
Black soldier fly manure;Composting;Heavy metal;Pathogen;Pollution reduction
摘要:
The bioconversion of black soldier fly (BSF) is a new model of livestock manure resourcing. However, the biochemical properties of BSF manure are unstable and direct application can be harmful to crops. Therefore, the effect of additives (biochar, humic acid and tea residue) on the removal of heavy metals and pathogens from BSF compost was investigated. Biochar inhibited the availability of Zn (58.9 %) and As (51.7 %) more significantly. Humic acid and tea residue significantly reduced the availability of Zn (60.8 %) and As (42.5 %) respectively. Humic acid and tea residue inhibited the bioavailability index of heavy metals more than biochar. At the end of composting, the total number of pathogenic bacteria was reduced by 80.1–96.0 % and pathogenic fungi by 41.4–99.9 %. Humic acid and biochar are more helpful in inhibiting the growth of pathogens. The abundance of dominant pathogenic genera was reduced by additive modulation. OM, EC, and temperature were the most key factors affecting the pathogenic bacteria. OM, pH, EC, Cu, Zn, and Cr also responded significantly to the pathogenic fungi. This study promotes the efficient conversion of livestock manure via BSF and provided theoretical guidance for the removal of pollutants in compost.
The bioconversion of black soldier fly (BSF) is a new model of livestock manure resourcing. However, the biochemical properties of BSF manure are unstable and direct application can be harmful to crops. Therefore, the effect of additives (biochar, humic acid and tea residue) on the removal of heavy metals and pathogens from BSF compost was investigated. Biochar inhibited the availability of Zn (58.9 %) and As (51.7 %) more significantly. Humic acid and tea residue significantly reduced the availability of Zn (60.8 %) and As (42.5 %) respectively. Humic acid and tea residue inhibited the bioavailability index of heavy metals more than biochar. At the end of composting, the total number of pathogenic bacteria was reduced by 80.1–96.0 % and pathogenic fungi by 41.4–99.9 %. Humic acid and biochar are more helpful in inhibiting the growth of pathogens. The abundance of dominant pathogenic genera was reduced by additive modulation. OM, EC, and temperature were the most key factors affecting the pathogenic bacteria. OM, pH, EC, Cu, Zn, and Cr also responded significantly to the pathogenic fungi. This study promotes the efficient conversion of livestock manure via BSF and provided theoretical guidance for the removal of pollutants in compost.
摘要:
At present, there are few studies on seasonal differences in the aroma quality and volatile components of Rucheng Baimao (Camellia pubescens) black tea. In this study, sensory evaluation and volatile component analysis were carried out on one sample of Rucheng Baimao black tea corresponding to spring, summer, and autumn, respectively. The results of sensory evaluation showed that the black teas of all three seasons had floral aromas. However, the aroma quality of spring black tea was the best, followed by that of autumn black tea, and summer black tea was the worst. The analysis of volatile components showed that alcohols, esters, and alkanes were the main substance categories. In addition, the results of the aroma index were consistent with those of the sensory evaluation, indicating that spring black tea had the best aroma quality, followed by autumn black tea and then summer black tea. Eleven key differential volatile components were screened by combining PLS-DA analysis (VIP > 1, p < 0.05) and rOAV > 1. Among them, geraniol, methyl salicylate, nonanal, and (E)-citral accumulated the most in spring black tea, linalool, phenylacetaldehyde, benzaldehyde, phenethyl alcohol, benzyl alcohol, and β-ionone accumulated the most in summer black tea, and trans-nerolidol accumulated the most in autumn black tea. This study aims to provide a theoretical reference for the regulation of the aroma quality of Rucheng Baimao black tea.