摘要：
Summer-autumn tea leaves are abundant, yet their utilization remains low. Our study enhances the quality and flavor of Golden Flower loose tea (GFLT) from fresh summer-autumn tea leaves by using a special "Golden Flower" fungus (strain ACF-2). The strain ACF-2, identified as Aspergillus cristatus with yellow cleistothecium and isolated from Fu Brick Tea, was characterized by colony morphology, microstructural analyses, and phylogenetic examination using a 3-gene dataset (BenA, CaM, RPB2). Inoculation of raw dark tea products with A. cristatus led to a significant increase in tea water extract and a reduction in tea polyphenols, soluble sugars, flavonoids, and total amino acids, thereby enhancing the quality and flavor of GFLT. HS-SPME-GC-MS analysis of GFLT aroma revealed that A. cristatus substantially improved the tea's aroma profile. 9 volatile compounds-(Z)-jasmone I, beta-cyclocitral, linalool oxide I, linalool, hexanal, 1-octen-3-ol, (Z)-citral, citral, and methyl salicylate, were found to be significantly elevated in GFLT compared to the controls. Our findings provide both theoretical and practical insights into optimizing the utilization of summer-autumn tea leaves, identifying the "Golden Flower" fungus, and understanding its impact on GFLT quality. In summary, fermenting A. cristatus to produce GFLT may be a new channel to utilize summer-autumn tea.
摘要：
Overexpression of OsSTP1 enhances the non-structural carbohydrate remobilization in the source, starch accumulation in grains, and the transportation of carbohydrates from source to sink during the filling stage. The sugar transporter protein (STP) is the best-characterized subfamily of the monosaccharide transporter (MST) family and plays critical roles in regulating plant stress tolerance, growth, and development. However, the role of STPs in regulating rice yield is poorly understood. In this study, we report that compared with Taipei 309, overexpression of OsSTP1 can achieve higher rice yield. We demonstrate that OsSTP1 mRNA levels are higher than those of the other seven STPs in mixed samples of leaf sheaths, stems, and nodes at 12days after pollination (DAP). OsSTP1 is prominently expressed in the leaf sheaths, stems, and nodes at the grain filling stage. Subcellular localization analysis revealed that OsSTP1 is localized in the plasma membrane. Overexpression of OsSTP1 increased the activities of amylase (AMY) and sucrose phosphate synthase (SPS) in mixed samples of leaf sheaths, stems, and nodes at 12 DAP, the sucrose content of the phloem exudate, and accumulation of soluble sugars and starch in panicles, ultimately increasing seed-setting rates and grain yields in the Taipei 309 cultivar. These findings indicate that overexpression of OsSTP1 can improve grain yield by synergistically promoting non-structural carbohydrate (NSC) remobilization and transportation.
作者机构：
[Liu, Zhi-Wei; Tang, Pan-Pan; Tan, Yi-Cheng; Liu, Tian-Le] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Liu, Xiu-Bin] College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Liu, Chang; Cheng, Jun-Hu] School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China;[Aadil, Rana Muhammad] National Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, Pakistan
通讯机构：
[Zhi-Wei Liu] C;College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China
摘要：
The potential of cold plasma (CP) treatment to promote the formation of amyloid fibrils (AFs) of ovalbumin (OVA) was evaluated relative to acidic heat fibrillation condition (pH = 2, 85 °C). Results indicated CP exhibited significant potential for promoting the formation of AFs of OVA, as evidenced by higher Thioflavin T (ThT) fluorescence intensity of AFs for CP-treated OVA (POVA) during the fibrillation process compared to AFs for native OVA under acidic heating conditions (NOVA). Long, curved and worm-like fibrils of CP-treated OVA with 2 min (POVA-2), which were thicker (3 nm in height) and longer (majority length ranging from 300 nm to 400 nm), were observed after 8 h of fibrillation, while irregular, short, worm-like fibrils of NOVA were detected. Unlike NOVA, which was only hydrolyzed into small peptides, the backbone of POVA-2 was cleaved into small peptide fragments, accompanied by the generation of dityrosine cross-linked aggregation/oligomer during fibrillation process, as evidenced by SDS-PAGE and dityrosine analysis. The result of hydrophobic, sulfhydryl and disulfide bonds, and dityrosine analysis showed hydrophobic interaction and dityrosine cross-links could be the main force driving the assembly and stacking of cross-β structures, leading to the formation of organized fibrillar structures for POVA-2, while only hydrophobic interaction was involved for NOVA. Additionally, analyses of emulsifying ability (EAI) and stability (ESI) of NOVA and POVA-2 displayed that both EAI and ESI of them were significantly improved, with POVA-2 exhibiting superior EAI compared to NOVA. Therefore, this study demonstrated CP is a promising technique to promote the generation of protein amyloid fibrils in a more efficient manner.
作者机构：
[Xiangyu Pi; Mingzhu Pan; Zisheng Wang; Zhitao Qi] Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng, China;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China;College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China;[Yang Xu] College of Fisheries, Hunan Agricultural University, Changsha, China;Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, China
通讯机构：
[Mingxian Chang] S;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China<&wdkj&>College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China<&wdkj&>Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, China
作者机构：
[Lu, Zhengji; Sheng, Zhaoyue; Zhou, Hui; Luo, Jie] College of Food Science and Technology, Hunan Agricultural University, Changsha 410114, China;[He, Jian; Zhang, Xiaoying; Wang, Caiyun] National Center of Technology Innovation for Dairy, Hohhot, China;[Zhang, Ying] Inner Mongolia Yijiahao Cheese Co., Ltd., Hohhot, China
通讯机构：
[Jie Luo] C;College of Food Science and Technology, Hunan Agricultural University, Changsha 410114, China
摘要：
Processed cheese analogues (PCA) are popular among consumers worldwide for their diverse flavors and forms. Casein/κ-carrageenan (CA/KC), as the main structural basis of common PCA, suffers from syneresis and thermal instability, which could be well ameliorated by locust bean gum (LBG) as a thickening, non-gelatinizing polysaccharide. However, the mechanism by which LBG improves the gel properties of CA/KC composites has not yet been clarified. In this study, the CA/KC/LBG ternary composite gels were constructed and the effect of LBG concentrations (0–0.5%) on the CA/KC binary gel properties were investigated. Compared with the control CA/KC gel, the addition of low LBG concentrations (0.1–0.3%) significantly increased the water-holding capacity, strength and thermal stability of gels (P < 0.05), while the addition of high LBG concentrations (0.4%–0.5%) weakened the gel properties. To reveal the influence mechanism of LBG, the water migration, molecular interactions and microstructure of gels were compared. The results confirmed that the addition of LBG induced the migration of water molecules from free state to bound state and enhanced the hydrogen bonding interactions between CA/KC gel molecules. In addition, scanning electron microscopy results showed that the addition of low concentrations of LBG induced the unfolding of KC macromolecules into chains, which optimized the gel network structure of KC. The formed CA/KC/LBG ternary composite gels showed more homogeneous and denser gel network structure than the control. In conclusion, the addition of an appropriate amount of LBG could well improve the thermal stability of CA/KC binary gel and the sense of storage stability of PCA products.
作者机构：
[Liu, Yuling; Ding, Siduo; Hu, Zhong; Tie, Baiqing; Luo, Si] College of Environment and Ecology, Hunan Agricultural University, Changsha, 410128, China;[Zeng, Haowei] School of Environment, South China Normal University, Guangzhou, 510006, China
通讯机构：
[Si Luo] C;College of Environment and Ecology, Hunan Agricultural University, Changsha 410128, China
作者：
Zeng, Wenqing;Yao, Bin;Zhou, Yaoyu;Yang, Jian;Zhi, Dan
期刊：
环境科学学报(英文版),2025年150:36-53 ISSN：1001-0742
通讯作者：
Yaoyu Zhou<&wdkj&>Dan Zhi
作者机构：
[Zeng, Wenqing; Yao, Bin; Zhou, Yaoyu; Yang, Jian; Zhi, Dan] College of the Environment and Ecology, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Yaoyu Zhou; Dan Zhi] C;College of the Environment and Ecology, Hunan Agricultural University, Changsha 410128, China
期刊：
Separation and Purification Technology,2025年356:129981 ISSN：1383-5866
通讯作者：
Nan Zhou
作者机构：
[Shi-Kai Li; Yu-Jiao Wen; Yi-Fan Wang; Meng Liu; Zheng-jie Peng; Le-zhu Su; Zhi Zhou; Nan Zhou] Hunan Engineering Research Center for Biochar, school of Chemistry and Materials Science, College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Nan Zhou] H;Hunan Engineering Research Center for Biochar, school of Chemistry and Materials Science, College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha 410128, China
作者机构：
[Qiaorong Li; Yao Liu; Ning Su; Lin Tan] Hunan Agricultural University, Changsha 410128, China;National Engineering Center for Efficient Utilization of Soil and Fertilizer Resources, Changsha, 410128, China;[Chang Tian; Yuping Zhang; Jianwei Peng; Xiangmin Rong; Gongwen Luo] Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Engineering Center for Efficient Utilization of Soil and Fertilizer Resources, Changsha, 410128, China
通讯机构：
[Lin Tan] H;Hunan Agricultural University, Changsha 410128, China
期刊：
Sensors and Actuators B: Chemical,2025年423:136709 ISSN：0925-4005
通讯作者：
Xingbo Shi<&wdkj&>Yan Lv<&wdkj&>Qian Zhao
作者机构：
Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Miao Zhang; Jixiang Zhou; Yiming Li; Qian Luo; Xiaoke Xiong; Xingbo Shi; Yan Lv; Qian Zhao] Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University,Changsha 410128, China
通讯机构：
[Xingbo Shi; Yan Lv; Qian Zhao] L;Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
关键词：
Forest succession;Community assembly;Co-occurrence;Deterministic process;Stochastic process;Karst region
摘要：
Understanding the assembly processes of soil microbial communities as secondary succession proceeds offers a critical insight into ecosystem recovery after disturbance. However, a comprehensive understanding of which ecological processes govern the assembly remains elusive. In this study, soil samples were sampled across four seasons (i.e., spring, summer, autumn, and winter) from various forest succession including shrubland, secondary forest, and primary forest, within a karst region in southwestern of China. The assembly of microbial communities was analyzed using the method of the null model, coupled with measurements of environmental variability. The results demonstrate that soil bacterial assembly is primarily dominated by the deterministic processes with their relative influence increases as karst forest proceeds; while soil fungal assembly is dominated by the stochastic processes, and the relative significance of stochasticity peaks in the secondary forest. Moreover, both soil bacterial and fungal communities' co-occurrence networks intensifies as forest succession. The shift in the balance between deterministic and stochastic across successional stages is predicted by factors such as plant DBH and soil nutrient availability. Specially, soil nitrate nitrogen (NO3 --N), along with plant diameter at breast height (DBH), available potassium (AK), available phosphorus (AP), and total phosphorus (TP), emerged as crucial determinants of soil microbial assembly as karst forest succeeds. Overall, our study provides the evidence that the bacterial and fungal communities' assembly vary within and across forest succession, and highlights the importance of plant properties and soil micro-environment for these community assembly in karst soil.
期刊：
Advanced Energy Materials,2024年14(3):2302712- ISSN：1614-6832
通讯作者：
Fu, Lijun;Wu, YP;Ozoemena, KI
作者机构：
[Ye, Jilei; Liu, Lili; Yuan, Xuelong; Fu, Lijun; Yuan, Xinhai; Zhang, Shuaishuai; Wu, Yuping; Fu, LJ] Nanjing Tech Univ, Coll Chem Engn, Sch Energy Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China.;[Chen, Peng] Northeastern Univ, Minist Educ, Key Lab Ecol Met Multimet Minerals, Sch Met, Shenyang 110819, Peoples R China.;[Wu, Xiongwei] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;[Wang, Tao; Wu, Yuping] Southeast Univ, Sch Energy & Environm, Confucius Energy Storage Lab, Nanjing 210096, Jiangsu, Peoples R China.;[Ozoemena, Kenneth I.; Ozoemena, KI] Univ Witwatersrand, Mol Sci Inst, Sch Chem, Private Bag 3, ZA-2050 Johannesburg, South Africa.
通讯机构：
[Wu, YP ; Fu, LJ] N;[Ozoemena, KI ] U;Nanjing Tech Univ, Coll Chem Engn, Sch Energy Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China.;Southeast Univ, Sch Energy & Environm, Confucius Energy Storage Lab, Nanjing 210096, Jiangsu, Peoples R China.;Univ Witwatersrand, Mol Sci Inst, Sch Chem, Private Bag 3, ZA-2050 Johannesburg, South Africa.
关键词：
Al metal;aqueous rechargeable Al-ion battery;cobalt hexacyanoferrate;high energy density
摘要：
An aqueous rechargeable Al‐ion battery (ARAIB) using a cubic phase cobalt hexacyanoferrate as positive electrode shows excellent rate and cycling performance together with an average discharge voltage of 1.56 V and an energy density of 155 Wh kg−1. Abstract Like other aqueous batteries, aqueous rechargeable Al‐ion batteries (ARAIBs) have attracted much attention due to their high safety and low cost. However, the low energy density of ARAIBs limits its popularization and application. In order to solve this problem, in addition to choosing Al metal as the negative electrode, it is also necessary to choose a suitable positive electrode material. Here, a cubic phase cobalt hexacyanoferrate (CoHCF) with excellent rate and cycling performance is used as the positive electrode material. Due to the reversible catalysis of the Cl−/Cl0 reaction at high potential in saturated AlCl3 solution, it has the characteristics of high capacity up to 103.5 mAh g−1. Combined with the Al metal as negative electrode, an ARAIB with an average discharge voltage of 1.56 V and an energy density of 155 Wh kg−1 is constructed, which shows outstanding cycling and rate performances.
摘要：
<jats:title>Abstract</jats:title>
<jats:p>The distribution of nutrients in sediments is the result of multiple factors, including hydrological conditions and vegetation regulation, and in wetlands with complex hydrological conditions, this distribution is uncertain. In this study, the spatial distribution patterns of nitrogen and phosphorus in sediments were studied in the riverside and lakeside wetlands of Dongting Lake, a typical river-connected lake. The results showed that the nitrogen and phosphorus concentrations in the surface sediments were higher than those in the subsurface sediments in both the riverside and lakeside wetlands. In addition, the concentration of total nitrogen (TN) of lakeside wetlands in the surface sediments was higher than that of riverside wetlands, whereas the concentration of total phosphorus (TP) did not differ between the two wetland types. In the surface sediments, there were significant positive correlations between the TN and TP concentrations in the riverside wetlands (<jats:italic>p</jats:italic> < 0.05), but no significant correlation in the lakeside wetlands (<jats:italic>p</jats:italic> > 0.05). In riverside wetlands, the nitrogen and phosphorus concentrations showed strong spatial dependence. However, in the lakeside wetlands, the spatial dependence of NO<jats:sub>3</jats:sub>
<jats:sup>−</jats:sup>-N was strong, that of NH<jats:sub>4</jats:sub>
<jats:sup>+</jats:sup>-N was moderate, and that of TN and TP was weak. This study shows that both hydrology and vegetation cause differences in the distribution of nutrients in the sediments. The results obtained from this investigation clarify the differences of sediment nitrogen and phosphorus distributions in the two types of wetlands and provide a technical reference for the management of different wetland types.</jats:p>
摘要：
The impact of global warming on plant abundance has been widely discussed, but it remains unclear how warming affects plant physiological traits, and how these traits contribute to the abundance of aquatic plants. We explored the adjustments in physiological traits of two common aquatic plant species (Potamogeton crispus L. and Elodea canadensis Michx.) and their links to plant abundance in three temperature treatments by determining twelve physiological traits and plant abundance over an 11-month period in outdoor mesocosms. This mesocosms facility has been running uninteruptedly for 16years, rendering the plants a unique opportunity to adapt to the warming differences. We found that 1) warming reduced the starch storage in winter for P. crispus and in summer for E. canadensis while increased the nitrogenous substances (e.g., TN, FAA, and proline) in winter for P. crispus. 2) For E. canadensis, TC, starch, SC, and sucrose contents were higher in summer than in winter regardless of warming, while TC, SC, and sucrose contents were lower in summer for P. crispus. 3) Warming decreased the association strength between physiological traits and plant abundance for P. crispus but enhanced it for E. canadensis. 4) E. canadensis showed increased interaction strength among physiological traits under warming, indicating increased metabolic exertion in the response to warming, which contributed to the reduction in abundance. Trait interaction strength of P. crispus was reduced under warming, but with less impact on plant abundance compared with E. canadensis. Our study emphasizes that warming alters the network of plant physiological traits and their contribution to abundance and that different strengths of susceptibility to warming of the various plant species may alter the composition of plant communities in freshwater ecosystems.
