Crop rotation is a common farmland management measure, which can effectively improve soil permeability and soil fertility, reduce crop diseases and pests, and affect microbial activity and community structure to a certain extent. However, the effects of long-term crop rotation pattern on nitrogen cycle microorganisms and crop yield are still unclear. Using monocrop rice as a control (CK), this study looked at the effects of different rotation years of tobacco rice (rotation years 10–20 (T10), 20–40 (T20), and >40 years of rotation) on nitroge...