Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

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成果类型：

期刊论文

作者：

Wei, Peng;Li, Jinhang;Liu, Xinyang;Nan, Can;Shi, Li;...

通讯作者：

He, Lin

作者机构：

[Li, Jinhang; He, Lin; Zhang, Yichao; Wei, Peng; Liu, Xinyang; Nan, Can; Li, Chuanzhen; Shi, Li] Southwest Univ, Acad Agr Sci, Chongqing 400716, Peoples R China.

[Li, Jinhang; He, Lin; Zhang, Yichao; Wei, Peng; Liu, Xinyang; Nan, Can; Li, Chuanzhen; Shi, Li] Southwest Univ, Coll Plant Protect, Chongqing 400716, Peoples R China.

[Shi, Li] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.

通讯机构：

[He, Lin] S

Southwest Univ, Acad Agr Sci, Chongqing 400716, Peoples R China.

Southwest Univ, Coll Plant Protect, Chongqing 400716, Peoples R China.

语种：

英文

关键词：

Tetranychus;pyrethroid resistance;carboxylesterase;RNAi;gene expression

期刊：

Pest Management Science

ISSN：

1526-498X

年：

2019

卷：

期：

页码：

252-261

DOI：

10.1002/ps.5109

基金类别：

This research was funded by Chongqing Research Program of Basic Research and Frontier Technology (No. cstc2017jcyiBx0061), the National Nature Sciences Foundation (31672085) and the China Scholarship Council (CSC).

机构署名：

本校为其他机构

院系归属：

植物保护学院

摘要：

BACKGROUND: Carboxylesterases (CarEs) are important in pesticide resistance. Four overexpressed CarE genes with inducible character were screened out in fenpropathrin-resistant Tetranychus cinnabarinus, but their functional roles remained to be further analyzed by RNAi and protein expression. RESULTS: Feeding a single double-stranded (ds)RNA of each of four genes led to gene-specific downregulation of mRNA, decreased esterase activity and diminished resistance in T. cinnabarinus. More interestingly, feeding four dsRNAs simultaneously led to a m...

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Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

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