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Effects of calcitonin on porcine intestinal epithelial cells proliferation, phosphorus absorption, and NaPi-IIb expression

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成果类型:
期刊论文
作者:
Tang, Xiaopeng;Su, Wen-qin;Fang, Re-jun*
通讯作者:
Fang, Re-jun
作者机构:
[Tang, Xiaopeng; Su, Wen-qin; Fang, Re-jun] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China
[Fang, Re-jun] Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China
通讯机构:
[Fang, Re-jun] H
Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China. Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
absorption;biological development;cell;gene expression;hormone;laboratory method;protein;sodium;Sus
期刊:
PAKISTAN JOURNAL OF ZOOLOGY
ISSN:
0030-9923
年:
2019
卷:
51
期:
6
页码:
2167-2174
基金类别:
This research was supported by grants from the Natural Science Foundation of Hunan Province (No. 2018JJ2163), and the National Natural Science Foundation of China (No. 31572419). We also thank Professor Olayiwola Adeola from Purdue University of America for his kindness revised of this manuscript.
机构署名:
本校为第一且通讯机构
院系归属:
动物科学技术学院
摘要:
To investigate the effects of calcitonin (CT) on cell proliferation, NaPi-IIb expression, and the absorption of phosphorus in porcine intestinal epithelial cells (IPEC-1). The experiment consisted of 1 control treatment and 5 levels of CT treatments (1×10-12 to 1×10-8 mol/L), and each treatment has 6 replications. Cell proliferation was determined by the MTT method. Cell morphology and phosphorus concentration in the cell supernatant were measured after 24 h of CT treatment. The NaPi-IIb protein expression was determined by Western Blot, and ...

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