Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350108, China
语种：
英文
关键词：
Cell viability;Grid-patterned DNA-protein hybrid (GDPH);Prevention of cancer metastasis;Three-dimensionally (3D) fluorescence counts;miR-21
National Natural Science Foundation of China (NSFC) [22174020, 21775024]; Key Project of Natural Science Foundation of Fujian Province [2019J02005]
机构署名：
本校为其他机构
院系归属：
生物科学技术学院
摘要：
Introduction: MicroRNAs (miRNAs) have been revealed to be critical genetic regulators in various physiological processes and thus quantitative information on the expression level of critical miRNAs has important implications for the initiation and development of human diseases, including cancers.Objectives: We herein develop three-dimensionally (3D) counting of intracellular fluorescent spots for accurately evaluating microRNA-21 (miRNA-21) expression in individual HeLa cells based on stimuli activated in situ growth of optical DNA flares, grid-patterned DNA-protein hybrids (GDPHs). Methods: T...
