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In Vitro Screening of Lactic Acid Bacteria with RAW264.7 Macrophages and the Immunoregulatory Mechanism

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成果类型:
期刊论文
作者:
Lu, Yuemei;Wu, Yanyang;Pan, Lina;Wang, Jiaqi;Tang, Rongxue;...
通讯作者:
Zhao, LY;Kang, WL
作者机构:
[Deng, Fangming; Zhao, Lingyan; Lu, Yuemei; Wu, Yanyang] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410127, Peoples R China.
[Kang, Wenli; Kang, WL; Tang, Rongxue; Wang, Jiaqi; Pan, Lina] Ausnutria Dairy China Co Ltd, Changsha 410200, Peoples R China.
[Kang, Wenli; Kang, WL; Wang, Jiaqi; Pan, Lina] Hunan Engn Res Ctr Human Microecol Prod, Changsha 410001, Peoples R China.
通讯机构:
[Kang, WL ] A
[Zhao, LY ] H
Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410127, Peoples R China.
Ausnutria Dairy China Co Ltd, Changsha 410200, Peoples R China.
Hunan Engn Res Ctr Human Microecol Prod, Changsha 410001, Peoples R China.
语种:
英文
关键词:
RAW264.7macrophages;immunomodulatory activity;LAB;NF-kappa B;in vitro
期刊:
Processes
ISSN:
2227-9717
年:
2024
卷:
12
期:
5
页码:
903-
基金类别:
This study was financially supported by the Science and Technology Innovation Program of Hunan Province (2022 RC1063); the National Natural Science Foundation of China (Project No. 31401675), the Hunan Provincial Department of Science and Technology Support Program (Project No. 2015NK3011 and 2016NK2110).
机构署名:
本校为第一且通讯机构
院系归属:
食品科学技术学院
摘要:
Lactic acid bacteria (LAB) are commonly consumed as probiotics to improve gut barrier function and boost the immune system. This study aimed to screen LAB with high immunomodulatory activity using RAW264.7 macrophages. According to the results, Limosilactobacillus reuteri AUc2301 was selected from 84 screened strains that can stimulate RAW264.7 cell proliferation. Limosilactobacillus reuteri AUc2301 significantly enhanced the phagocytosis activity of RAW264.7 cells. In the ELISA test, Limosilactobacillus reuteri AUc2301 significantly promoted the release of interleukin-6, IL-1 beta, the tumor ...

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